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capsid-targeted viral inactivation
相关语句
  核衣壳导向的病毒灭活
     The fusion protein gene of HBV core protein and human eosinophil derived neurotoxin, hereafter called HBV targeted ribonuclease gene(HBV-TR gene), was constructed based on the theory of capsid-targeted viral inactivation(CTVI ) .
     乙型肝炎病毒核心蛋白和人嗜酸性粒细胞来源神经毒素融合蛋白基因,或称为乙型肝炎病毒靶向核糖核酸酶基因(HBV targeted ribonuclease gene,以下简称HBV-TR基因)是我室刘军博士等根据核衣壳导向的病毒灭活原理(capsid-targeted viral inactivation,CTVI)构建的融合基因。
短句来源
  “capsid-targeted viral inactivation”译为未确定词的双语例句
     Capsid-targeted viral inactivation for dengue virus infection
     衣壳蛋白靶向灭活策略应用于抗登革病毒感染的研究
短句来源
     To explore the feasibility of capsid-targeted viral inactivation for dengue virus infection, a newly-discovered antiviral strategy, a mammalian cell line stably expressing staphylococcal nuclease fused to the capsid protein of dengue 2 virus was established and the effects on the production of infectious virus particles were examined.
     衣壳蛋白靶向病毒灭活是近年来新兴的抗病毒策略。 为探索该策略在抗登革病毒感染中的应用 ,首先建立了稳定表达登革 2型病毒衣壳蛋白 (D2C)与葡萄球菌核酸酶 (SN)融合蛋白D2C_SN的哺乳动物细胞系 ,然后以登革病毒攻击上述细胞系 ,研究表达的融合蛋白D2C_SN对产生的子代病毒颗粒感染性的影响。
短句来源
     Capsid-targeted Viral Inactivation:A New Antiviral Strategy
     衣壳蛋白靶向灭活——一种新型抗病毒策略
短句来源
     Capsid-targeted viral inactivation (CTVI) is a new antiviral strategy, in which nucleases fused to viral coat proteins are expressed in infected cells and become incorporated during virion assembly, and degrade viral nucleic acids. The nucleases commonly used in CTVI are SN nuclease from Staphylococcus, Barnase from Bacillus amyloliquefaciens and RNase HI from E. coli.
     衣壳蛋白靶向灭活(CTVI)是一种新型抗病毒策略,它是通过将病毒衣壳蛋白与核酸酶(金黄色葡萄球菌核酸酶、核糖核酸酶Barnase、大肠杆菌RNase HI等)的融合蛋白装配到病毒粒子中,使核酸酶接触并降解病毒核酸,从而达到抑制病毒复制的目的。
短句来源
  相似匹配句对
     Capsid-targeted viral inactivation for dengue virus infection
     衣壳蛋白靶向灭活策略应用于抗登革病毒感染的研究
短句来源
     Study of Dengue Virus Capsid Targeted Viral Inactivation
     登革病毒衣壳蛋白靶向抗病毒作用的研究
短句来源
     Capsid-targeted Viral Inactivation:A New Antiviral Strategy
     衣壳蛋白靶向灭活——一种新型抗病毒策略
短句来源
     The results paved exploring the feasibility of capsid targeted viral inactivation strategy in anti-dengue infection.
     这些结果为进一步将衣壳蛋白靶向病毒灭活策略应用于人类抗登革病毒感染奠定了基础
短句来源
     Viral inactivation of split influenza virus vaccine
     流行性感冒病毒裂解疫苗灭活试验探讨
短句来源
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  capsid-targeted viral inactivation
Capsid-targeted viral inactivation (CTVI) has emerged as a conceptually powerful antiviral strategy that exploits viral structural proteins to target a destructive enzyme specifically into progeny virions.
      
Capsid-targeted viral inactivation can destroy dengue 2 virus from within in vitro
      


Based on the nucleotide sequence of the dengue 2 virus capsid protein C gene and staphylococcal nuclease(SN)gene,a pair of primers was designed to amplify the desired fusion gene CSN from the constructed plasmid pLEX/D2C-SN.The fusion gene encoding dengue C gene and SN gene was then cloned into eukaryotic vector pcDNA6/V5-His to generate the desired recombinant plasmid pcDNA/D2C-SN. After transfected into the BHK cells by electroporation,the resistant clones were screened by RT-PCR. The transfected cells were...

Based on the nucleotide sequence of the dengue 2 virus capsid protein C gene and staphylococcal nuclease(SN)gene,a pair of primers was designed to amplify the desired fusion gene CSN from the constructed plasmid pLEX/D2C-SN.The fusion gene encoding dengue C gene and SN gene was then cloned into eukaryotic vector pcDNA6/V5-His to generate the desired recombinant plasmid pcDNA/D2C-SN. After transfected into the BHK cells by electroporation,the resistant clones were screened by RT-PCR. The transfected cells were selected by 5 mg/L blasticidin and analyzed by indirected immunofluorescence and Western blot. The activity of nuclease of expressed fusion protein D2C-SN was analyzed in an in vitro DNA digestion assay.It was confirmed that a BHK cell line expressing the fusion protein D2C-SN was obtained. The fusion protein has ideal nuclease activity and no cytotoxicity on host cell. The results paved exploring the feasibility of capsid targeted viral inactivation strategy in anti-dengue infection.

根据登革 2型病毒衣壳蛋白C基因和葡萄球菌核酸酶SN基因序列设计引物 ,从构建的原核表达载体pLEX D2C SN中扩增获得编码登革病毒衣壳蛋白和葡萄球菌核酸酶的融合基因D2C SN ,将其插入到真核表达载体pcDNA6 V5 His中 ,筛选获得重组质粒pcDNA D2C SN .电穿孔转染BHK细胞后 ,5mg Lblasticidin压力筛选 ,通过RT PCR、间接免疫荧光和免疫印迹鉴定表达的蛋白 ,体外DNA消化试验检测核酸酶活性 .结果表明 ,融合蛋白D2C SN在BHK细胞中获得了稳定表达 ,表达的融合蛋白能够被抗登革病毒衣壳蛋白的单克隆抗体特异识别 ,并具有良好的核酸酶活性 ,能够对DNA进行切割 .同时 ,BHK细胞中稳定表达的融合蛋白D2C SN能够有效抑制登革病毒的增殖 ,使其感染性降低 10 3 ~ 10 4倍 .这些结果为进一步将衣壳蛋白靶向病毒灭活策略应用于人类抗登革病毒感染奠定了基础

To explore the feasibility of capsid-targeted viral inactivation for dengue virus infection, a newly-discovered antiviral strategy, a mammalian cell line stably expressing staphylococcal nuclease fused to the capsid protein of dengue 2 virus was established and the effects on the production of infectious virus particles were examined. The results presented evidence that the enzymatically active staphylococcal nuclease fused to capsid protein could be incorporated into the nascent virions during wild virus...

To explore the feasibility of capsid-targeted viral inactivation for dengue virus infection, a newly-discovered antiviral strategy, a mammalian cell line stably expressing staphylococcal nuclease fused to the capsid protein of dengue 2 virus was established and the effects on the production of infectious virus particles were examined. The results presented evidence that the enzymatically active staphylococcal nuclease fused to capsid protein could be incorporated into the nascent virions during wild virus assembly, resulting in degradation of viral genomic RNA and decrease in infectivity. Comparing the effects of incorporated SN and SN *, an enzymatically inactive missense mutant form of SN, on the infectivity of progeny virions, nucleolytic activity of incorporated SN was responsible for the major antiviral effects. These results paved the road of developing capsid-targeted viral inactivation as a new antiviral strategy against dengue.

衣壳蛋白靶向病毒灭活是近年来新兴的抗病毒策略。为探索该策略在抗登革病毒感染中的应用 ,首先建立了稳定表达登革 2型病毒衣壳蛋白 (D2C)与葡萄球菌核酸酶 (SN)融合蛋白D2C_SN的哺乳动物细胞系 ,然后以登革病毒攻击上述细胞系 ,研究表达的融合蛋白D2C_SN对产生的子代病毒颗粒感染性的影响。结果表明融合蛋白D2C_SN能够在病毒装配过程中与野生型衣壳蛋白共组装入子代病毒颗粒内部 ,并导致病毒基因组的降解。与正常BHK细胞相比较 ,融合蛋白D2C_SN可导致产生的子代病毒感染性滴度降低 10 3~ 10 4 ,显示出很强的抗病毒效果

Capsid-targeted viral inactivation (CTVI) is a new antiviral strategy, in which nucleases fused to viral coat proteins are expressed in infected cells and become incorporated during virion assembly, and degrade viral nucleic acids. The nucleases commonly used in CTVI are SN nuclease from Staphylococcus, Barnase from Bacillus amyloliquefaciens and RNase HI from E. coli. CTVI has successful applications in HIV-1 hepatitis B virus, murine leukaemia virus, Dengue virus, etc.The principle, advantages,...

Capsid-targeted viral inactivation (CTVI) is a new antiviral strategy, in which nucleases fused to viral coat proteins are expressed in infected cells and become incorporated during virion assembly, and degrade viral nucleic acids. The nucleases commonly used in CTVI are SN nuclease from Staphylococcus, Barnase from Bacillus amyloliquefaciens and RNase HI from E. coli. CTVI has successful applications in HIV-1 hepatitis B virus, murine leukaemia virus, Dengue virus, etc.The principle, advantages, applications and prospect of CTVI were mainly described.

衣壳蛋白靶向灭活(CTVI)是一种新型抗病毒策略,它是通过将病毒衣壳蛋白与核酸酶(金黄色葡萄球菌核酸酶、核糖核酸酶Barnase、大肠杆菌RNase HI等)的融合蛋白装配到病毒粒子中,使核酸酶接触并降解病毒核酸,从而达到抑制病毒复制的目的。该策略已经在人免疫缺陷病毒、鼠白血病病毒、乙肝病毒、登革病毒等抗病毒研究中取得良好效果,展示了广阔的应用前景。

 
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