助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   hepatitis delta virus 的翻译结果: 查询用时:0.158秒
图标索引 在分类学科中查询
所有学科
基础医学
生物学
感染性疾病及传染病
临床医学
消化系统疾病
更多类别查询

图标索引 历史查询
 

hepatitis delta virus     
相关语句
  丁型肝炎病毒
     Methods Hepatitis delta virus' sequence was obtained from Inner Mongolian patient by using RT-PCR and PCR methods, PET43a was used and His-tag was added at the HDV L-Ag 5′ and 3′ to construct the recombinant expression plasmid, transform the plasmid into host bacterium BL21 and induce it with IPTG.
     方法从内蒙古某丁肝患者血清中提取丁肝病毒,经RT-PCR与PCR,使用PET43a表达质粒及HDVL-Ag5′和3′端引入His标签获得丁型肝炎病毒L-Ag重组表达质粒,转化BL21(Rosetta)宿主菌,IPTG诱导表达。
短句来源
     The serum of 151 cases of gravidas with positive Hepatitis B Virus Marker (HBV -M )and unchangable liver function was detected for Hepatitis Delta Virus Marker (HDV-M ) byEnzyme Immune Assay(ElA). Theresults demonstrated that 24 cases showed HDV-M (+)(24/151, 15. 89% ).
     本文采用酶免疫测定法(EIA),检测长春地区151例乙肝病毒标志物(HBV-M)阳性,肝功能无改变的孕妇血清中丁型肝炎病毒标志物(HDV-M),结果HDV-M(+)共24例,阳性率15.89%(24/151)。
短句来源
     Study on the Inhibition of Hepatitis B Virus (HBV) by Hepatitis Delta Virus (HDV) Ribozyme in Cells
     丁型肝炎病毒(HDV)核酶对乙型肝炎病毒(HBV)抑制作用的研究
短句来源
     EXPERIMENTAL STUDIES OF EASTERN WOODCHUCK INFECTED WITH HEPATITIS DELTA VIRUS
     丁型肝炎病毒感染东方土拔鼠的实验研究
短句来源
     In Vitro Transcription of cDNA of Hepatitis Delta Virus Genomic RNA Ribozyme
     丁型肝炎病毒基因组RNA中核酶区的cDNA的体外转录
短句来源
更多       
  “hepatitis delta virus”译为未确定词的双语例句
     ESTABLISHMENT AND APPLICATION OF DETECTIVE METHOD OF IgM ANTIBODY TO HEPATITIS DELTA VIRUS
     丁型肝炎IgM抗体检测方法的建立和应用
短句来源
     The plasmid was constructed containing full-length antigenome cDNA of CC-47 that was under control of T7 promoter and joined with hepatitis delta virus ribozyme sequence.
     克隆麻疹病毒CC-47株蛋白N、P、L编码区质粒并置于T7启动子控制下,用4个质粒共转染哺乳动物细胞,在表达T7RNA聚合酶的重组痘苗病毒VTF7-3的作用下进行病毒拯救。
短句来源
     The antibody to hepatitis delta virus (Anti-HD) from the serum positive for HBsAg and Anti-HD was fractionated with ion-exchange chromatography of DE-52 and labelled with horseradish peroxidase (HRP).
     取乙型肝炎患者Delta抗体(抗—HD)阳性血清,经硫酸铵盐析和DE—52柱层析,得到纯化的抗—HD抗体,用辣根过氧化物酶标记。
短句来源
     [Objective] In order to study quality of recombinant antigen of hepatitis delta virus (HDV) and its difference from other HDV antigens.
     [目的]为了研究重组HDV抗原的性质及与其它HDV抗原的差别。
短句来源
     Basing on the mechanism of capture ELISA,we developed a specific andsensitive method for detecting IgM antibody to hepatitis delta virus ( IgM anti-HD ) withmouse monoclone antibody to IgM μchain and antigen of hepatitis delta virus (HDAg),etc.
     本文根据ELISA抗体捕获法的原理,采用抗人IgM单克隆抗体和丁型肝炎抗原等,建立了特异性强、灵敏度高的IgM抗-HD检测方法。
短句来源
更多       
  相似匹配句对
     STUDIES ON CELLULAR IMMUNITY OF DELTA HEPATITIS
     HDV感染者细胞免疫状态的研究
短句来源
     The Molecular Biology of Hepatitis delta virus
     丁型肝炎病毒的分子生物学研究进展
短句来源
     EXPRESSION OF CHINESE HEPATITIS DELTA ANTIGEN IN E.COLI
     中国丁型肝炎病毒四川株的抗原表达及其某些特征的研究
短句来源
     PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO HEPATITIS DELTA VIRUS
     抗丁型肝炎病毒单克隆抗体的制备及其生物学特性
短句来源
     Experimental studies of pockmark ducks infected by hepatitis delta virus
     HDV感染麻鸭的实验研究
短句来源
查询“hepatitis delta virus”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  hepatitis delta virus
The objective of this study was to estimate the presence of hepatitis delta virus RNA in chronically HBV-infected patients from northern Poland.
      
Hepatitis delta virus infection in chronically HBV-infected patients from northern Poland
      
Serum samples obtained from 289 first-time and 114 repeat donors at the Blood Center of Mongolia (MBC) were tested for serological and molecular markers of hepatitis B virus (HBV), hepatitis C virus (HCV), and hepatitis delta virus (HDV) infections.
      
The delta antigen of hepatitis delta virus exhibits sequence specific binding to its own RNA and is essential for viral replication.
      
Development and Characterisation of Recombinant Hepatitis Delta Virus-like Particles
      
更多          


Anti-hepatitis delta virus(HDV)antibody in various HBsAg positive population has been investigated with Abbott EIA assay.Eighteen of 1004 sera were anti-HDV positive,with a percentage of 1.8.The anti-HDV positive samples of 622 asympt-omatic HBsAg carriers,58 acute hepatitis B patients, 241 chronis hepatitisB patients,and 83 primary liver cancer patients were 4(0.6%),0,4(1.7%),and 10(12.1%)respectively.Detection rate of anti- HDV was not related to the age and sex of the HBsAg positive population....

Anti-hepatitis delta virus(HDV)antibody in various HBsAg positive population has been investigated with Abbott EIA assay.Eighteen of 1004 sera were anti-HDV positive,with a percentage of 1.8.The anti-HDV positive samples of 622 asympt-omatic HBsAg carriers,58 acute hepatitis B patients, 241 chronis hepatitisB patients,and 83 primary liver cancer patients were 4(0.6%),0,4(1.7%),and 10(12.1%)respectively.Detection rate of anti- HDV was not related to the age and sex of the HBsAg positive population. The titers of these anti-HDV antibody in the sera were less than 1:10.The sera reexamination 2 after to 6 months were turned into negative anti-HDV results.The studysuggests that the incidence of hepatitis delta infection is very low in China.

采用Abbott EIA法对各种HBsAg阳性人群血清抗HDV抗体进行了调查,结果在1004份血清标本中检出有18份标本抗HDV抗体阳性,阳性率为1.8%。抗HDV抗体检出率与年龄、性别无关。但在无症状HBsAg携带者、急性乙型肝炎、慢性乙型肝炎(包括慢性活动性肝炎、慢性迁延性肝炎、肝硬化)、肝癌患者血清中,抗HDV抗体检出率有显著性差别,分别为0.6%、0%、1.7%、和12.1%。这些阳性血清中抗HDV抗体滴度<1:10,间隔2~6个月后对部份患者重新采血复查时,抗HDV抗体转为阴性。研究结果提示了低感染率的D型肝炎在我国的存在。

Hepatitis delta virus(HDV) is a defective single-strand RNA virus that requires hepatitis B virus for its replication and occurs in patient with acute and chronic HBV infection.We have established six hybri doma cell lines by fusion immunized mouse spleen cells with SP2/0 myeloma cell. All of them secret monoclonal antibody against HDAg that can be used to develop diagnostic reagents and analyse the anti genie domains of HDV.The titer of McAb in culture fluid and ascites fluids were 1:50...

Hepatitis delta virus(HDV) is a defective single-strand RNA virus that requires hepatitis B virus for its replication and occurs in patient with acute and chronic HBV infection.We have established six hybri doma cell lines by fusion immunized mouse spleen cells with SP2/0 myeloma cell. All of them secret monoclonal antibody against HDAg that can be used to develop diagnostic reagents and analyse the anti genie domains of HDV.The titer of McAb in culture fluid and ascites fluids were 1:50 to 1: 1,000 and 1:1,000 to 1 : 40,000 respectively. Four of them were IgG1, one was IgG2a and another was IgG mouse immu noglobulin. McAbs can react with HDAg in serum ( S-HDAg ) or expressed in E.coli(E-HDAg)but did not react with extract of E.coli,HB cAg and HBsAg expressed in E.coli, HBsAg positive serum and normal human serum.The reaction of McAbs with HDAg can be blocked by anti-HD positive human serum. This result shows that these McAbs were specific for HDAg. McAbs were used as a coating antibody for the detection of HDAg and Anti-HD in sera and compared with the results using polyclonal antibody. The coincident rate were 94.44% and 98.68% respectively.The result of detecting E-HDAg by McAb-EIA was much better than PcAb-EIA. We have found at least three antigenic determinatants on E-HDAg by McAbs to HDAg.The results also showed that the conformational structure of E-HDAg may be different from that of S-HDAg by analysis of antigenic domains with McAbs.

应用杂交瘤技术,以大肠杆菌基因工程表达的丁型肝炎病毒抗原(HDAg)免疫Balb/C小鼠,首次建立了6株分泌抗丁型肝炎病毒(HDV)单克隆抗体细胞株。它们的培养上清和腹水的抗体滴度分别是50~1000和1000~40000。Ig亚类4株为IgG1,1株IgG2a,1株IgG。这些单克隆抗体能和HDAg结合,而不与大肠杆菌裂解液,大肠杆菌表达的HBcAg、HBeAg,以及HBsAg阳性血清和正常人血清发生反应。它们与HDAg的结合能被抗HD阳性血清所阻断,说明这种结合是特异的。这些McAb的特异性和亲和性与多克隆抗体相同。以McAb做包被抗体和抗HDV多克隆酶标抗体配对制成EIA试剂,检测血清标本中的HDAg和抗-HD,与PcAbEIA的符合率分别为94.44%和98.68%。以PcAb包被,配以酶标记的McAb;或以McAb包被,配以酶标记的PcAb均可检测大肠杆菌表达的HDAg,而且效果都优于PcAb-EIA。用这些McAb对国外基因工程表达的HDAg进行的抗原位点分析首次证明,在这些HDAg上至少有3个不同的抗原位点。通过对基因工程表达的HDAg和我国人血清中HDAg的抗原位点的分析,认为两者至少在空间构...

应用杂交瘤技术,以大肠杆菌基因工程表达的丁型肝炎病毒抗原(HDAg)免疫Balb/C小鼠,首次建立了6株分泌抗丁型肝炎病毒(HDV)单克隆抗体细胞株。它们的培养上清和腹水的抗体滴度分别是50~1000和1000~40000。Ig亚类4株为IgG1,1株IgG2a,1株IgG。这些单克隆抗体能和HDAg结合,而不与大肠杆菌裂解液,大肠杆菌表达的HBcAg、HBeAg,以及HBsAg阳性血清和正常人血清发生反应。它们与HDAg的结合能被抗HD阳性血清所阻断,说明这种结合是特异的。这些McAb的特异性和亲和性与多克隆抗体相同。以McAb做包被抗体和抗HDV多克隆酶标抗体配对制成EIA试剂,检测血清标本中的HDAg和抗-HD,与PcAbEIA的符合率分别为94.44%和98.68%。以PcAb包被,配以酶标记的McAb;或以McAb包被,配以酶标记的PcAb均可检测大肠杆菌表达的HDAg,而且效果都优于PcAb-EIA。用这些McAb对国外基因工程表达的HDAg进行的抗原位点分析首次证明,在这些HDAg上至少有3个不同的抗原位点。通过对基因工程表达的HDAg和我国人血清中HDAg的抗原位点的分析,认为两者至少在空间构型上有不同。

The antibody to hepatitis delta virus (Anti-HD) from the serum positive for HBsAg and Anti-HD was fractionated with ion-exchange chromatography of DE-52 and labelled with horseradish peroxidase (HRP). Hepatitis delta antigen (HDAg) from the liver of the woodchuck hepatitis virus (WHV) carrier (woodchuck infected with HDV) was purified through Sepharose 4B column and affinity chromatography column. Its titre was 10~(-6) determined with double antibody sandwich method. Anti-HD in...

The antibody to hepatitis delta virus (Anti-HD) from the serum positive for HBsAg and Anti-HD was fractionated with ion-exchange chromatography of DE-52 and labelled with horseradish peroxidase (HRP). Hepatitis delta antigen (HDAg) from the liver of the woodchuck hepatitis virus (WHV) carrier (woodchuck infected with HDV) was purified through Sepharose 4B column and affinity chromatography column. Its titre was 10~(-6) determined with double antibody sandwich method. Anti-HD in serum could be detected with ELISA competitive method. The results of specific, sensitive and repeating tests of the reagent were identical with those of Delta assay Ab (Noctech Co., Ltd. Ireland)

取乙型肝炎患者Delta抗体(抗—HD)阳性血清,经硫酸铵盐析和DE—52柱层析,得到纯化的抗—HD抗体,用辣根过氧化物酶标记。用丁型肝炎病毒(HDV)感染慢性携带土拨鼠肝炎病毒的东方土拨鼠(Woodchuck)后,取其肝组织制成匀浆,离心后经Sepharose 4B柱,免疫亲和层析柱,获得高丰度的HDAg。双抗体夹心法测定其滴度高达1∶10~6。制备成ELISA竞争抑制法检测抗HD试剂盒,其特异性、敏感性、重复性与爱尔兰同种试剂比较结果一致。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关hepatitis delta virus的内容
在知识搜索中查有关hepatitis delta virus的内容
在数字搜索中查有关hepatitis delta virus的内容
在概念知识元中查有关hepatitis delta virus的内容
在学术趋势中查有关hepatitis delta virus的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社