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   restriction-analysis 的翻译结果: 查询用时:0.142秒
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内分泌腺及全身性疾病
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restriction-analysis
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  酶切分析
     Methods:We screen the codon 935 of exon 12 in ATP7B gene of 48 WD patients and 30 controls by PCR-augmentation and restriction-analysis with restriction enzyme Mae Ⅱ.
     方法 :用限制性内切酶MaeⅡ对 48例患者及 30例正常人的ATP7B基因第 12外显子 935密码子PCR扩增后进行酶切分析
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  “restriction-analysis”译为未确定词的双语例句
     Object:To detect high freqency mutation pint in Chinese patients with Wilson disease (WD)by PCR-augmentation and restriction-analysis and to establish a fast gene diagnosis method for WD.
     目的 :应用PCR 酶切法对中国人WD患者ATP7B基因的高频突变点进行检测 ,企图建立一种能应用于临床的中国人WD快速基因诊断方法。
短句来源
     Conclusion:The codon 935 of exon 12 in ATP7B gene is one of the high frequency of mutation points in Chinese WD patients,PCR-augmentation and restriction-analysis is a fast gene diagnosis method for WD.
     PCR 酶切法可作为WD常用的基因诊断方法应用于症状前期患者的筛选及产前诊断。
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  相似匹配句对
     Promotion and Restriction
     发展与制约
短句来源
     On the Power Restriction
     权力制约初探
短句来源
     Power Restriction and Anti-corruption
     权力制衡与反腐败
短句来源
     Restriction Enzyme-Mediated Integration
     限制性内切酶介导的整合技术
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     C -- M. S analysis.
     —M. S.
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The 2.17 kb EcoRI/BamHI restriction fragment of the mtDNA from Beijing duck liver was cloned, using plasmid pAT153 as a vector and E. coli SK1592 as recipient cells. The recombinants have been identified by restriction analysis and Southern blot analysis. Moreover, the stability of the chimeric plasmids in recipient cells has been studied and the question of nonsuccessful cloning of another EcoRI/BamHI fragment (2.81 kb) of duck liver mtDNA is also discussed.

本文以质粒pAT153为载体,以E.coli SK1592为受体菌,将北京鸭肝脏mtDNA的2.17kb的EcoRI/BamHI限制片段进行了克隆,并对重组质粒作了限制性内切酶分析、Southern吸印与杂交分析。此外,还对重组体的一些性质作了初步研究,并对鸭肝mtDNA的另一个EeoRI/BamHI片段(2.81kb)不能被克隆的原因进行了讨论。

SalI restriction fragments of the spinach chlorplast DNA were cloned into the Sail site of pBR322 DNA. Altogether 52 recombinant transferments (Ap'Tc") were selected and each of them was screened by restriction analysis. It was proved by Southern blot and probe hybridization that the recombinant plasmid pSS104 contains the 4.1 kb SalI fragment of the spinach chloroplast genes have been mapped, on this fragment. The transcription products of the cloned fragment have not been discovered with E. coli in vivo system....

SalI restriction fragments of the spinach chlorplast DNA were cloned into the Sail site of pBR322 DNA. Altogether 52 recombinant transferments (Ap'Tc") were selected and each of them was screened by restriction analysis. It was proved by Southern blot and probe hybridization that the recombinant plasmid pSS104 contains the 4.1 kb SalI fragment of the spinach chloroplast genes have been mapped, on this fragment. The transcription products of the cloned fragment have not been discovered with E. coli in vivo system.

利用pBR 322衍生质粒DNA为载体,将菠菜叶绿体DNA的SalⅠ限制性片段插入质粒的Sal Ⅰ位点,获得52个含重组质粒的菌落(Ap~rTc~5)。并对每个克隆的质粒进行限制性内切酶分析,通过Southern吸印与探针杂交,证明了重组质粒pSS104含有的插入DNA是菠菜叶绿体DNA 4.1kb的SalⅠ片段。迄今在该片段尚未定位任何已知的叶绿体基因,用大肠杆菌的活体系统也未能发现这段DNA的转录产物,本文对此进行了讨论。

In this investigation, 17 mtDNAs of Chinese Han people were used. By restriction analysis, cleavage fragments of Hinc Ⅱ, BamHI and Pvu Ⅱ showed RFLP. In assessment of hereditary similarities among mtDNAs, the mean value of nucleotide diversity obtained was 0.0064. This is reasonably agreeable with 0.0036 (Brown, 1980), 0.004 (Cann, 1982) and 0.00417 (Horai, 1984), but is quite different from 0.02 obtained by Aquado (1983) who analysed the 900 bp of D-loop in the mtDNAs of 7 people. In the study of mutation mechanism,...

In this investigation, 17 mtDNAs of Chinese Han people were used. By restriction analysis, cleavage fragments of Hinc Ⅱ, BamHI and Pvu Ⅱ showed RFLP. In assessment of hereditary similarities among mtDNAs, the mean value of nucleotide diversity obtained was 0.0064. This is reasonably agreeable with 0.0036 (Brown, 1980), 0.004 (Cann, 1982) and 0.00417 (Horai, 1984), but is quite different from 0.02 obtained by Aquado (1983) who analysed the 900 bp of D-loop in the mtDNAs of 7 people. In the study of mutation mechanism, it was found in 6 cases that there were gains and losses of sites due to nucleo- tide substitution. 3 eases of restriction site gains caused by point mutation were discussed,

实验研究对象为17例中国汉族人的mtDNA。通过限制性分析发现3种限制酶(HincⅡ、BamHI和PvuⅡ)酶解片段有RFLP现象,在估计mtDNA之间遗传相似性时,所得到的核苷酸歧异频率的平均值为0.0064。这个值与Brown的0.0036、Cann的0.004和Hotrai的0.00417的值接近,而与Aquado对7个人mtDNA D-环区900个碱基对分析得到的0.02相差较大。在研究突变机制时,发现有6例由于核苷酸的替换引起了限制性位点的增减。文中对其中因点突变引起限制性位点增加的3例进行了讨论。

 
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