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primer extension preamplification
相关语句
  引物延伸预扩增
     Methods:Primer extension preamplification method was applied to preamplify the genome and PCR method was used to detect HCMV-DNA after single fetal nucleated red blood cells were isolated from 273 maternal blood samples.
     方法:从273例母体血标本中分离出单个胎儿有核红细胞,用引物延伸预扩增方法对其基因组预扩增,再用PCR方法检测HCMV-DNA。
短句来源
     Methods Single lymphocyte was isolated from the peripheral or umbilical cord blood samples of 22 clinically diagnosed Down's syndrome patients or fetus and 40 normal controls by micromanipulation techniques. Primer extension preamplification (PEP) and real-time FQ-PCR were used to amplify the S100B and DCSR1 located on chromosome 21, and GAPDH located on chromosome 12. Two pairs of ΔCT values were compared between the two groups.
     方法22例唐氏综合征患者及胎儿、40名正常对照外周血或脐血经梯度离心、显微操作分离单个淋巴细胞,应用引物延伸预扩增及实时荧光定量PCR技术扩增基因组12号染色体上GAPDH基因、21号染色体上S100B基因和DSCR1基因。
短句来源
     Methods The DNA template was extracted by QIAamp Blood DNA midi kit from 42 maternal (14~40 weeks) plasma. A Y-chromosome-specific repeat sequence DYZ3 gene was chosen to be amplified after primer extension preamplification (PEP) based-PCR.
     方法提取42例孕14~40周的产妇血浆中游离胎儿DNA,采用引物延伸预扩增(primerextensionpreamplification,PEP)后行PCR,特异性扩增其Y染色体重复序列(DYZ3)基因。
短句来源
     Methods Using primer extension preamplification (PEP) and nested polymerase chain reaction (PCR), a normal male SRY gene in maternal plasma DNA was detected in 65 gravidas.
     方法 采用引物延伸预扩增 (PEP)法及巢式PCR技术对 65例孕妇外周血血浆DNA进行正常男性SRY基因检测。
短句来源
     To determine the origin of the NRBC , the single NRBC was analysed by primer extension preamplification (PEP) and polymerase chain reaction (PCR).
     显微操作法获取 5例单个 NRBC行引物延伸预扩增 (PEP)和聚合酶链反应 (PCR) ,验证其胎儿细胞来源。
短句来源
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  扩增前引物延伸
     Detection of multiple loci in single cell by primer extension preamplification and nest PCR
     单细胞扩增前引物延伸多基因位点检测研究
短句来源
     Objective To search for the value of preliminary matching of Human leukocyte antigen (HLA) in single-cell by Primer extension preamplification (PEP) followed by multiplex nested polymerase chain reaction (MN-PCR).
     目的 探索单细胞扩增前引物延伸法 (PEP)全基因组扩增 ,后续多重巢式聚合酶链反应 (MN PCR)同步扩增人类白细胞抗原 (HLA) A、B和DR基因位点检测技术 ,进行配型预选研究的价值。
短句来源
     Objective To study the effect of modified improved primer extension preamplification (IPEP) on STR analysis of trace DNA.
     目的探讨改良扩增前引物延伸(IPEP)法对痕量DNA样本STR检测分型的效果。
短句来源
  “primer extension preamplification”译为未确定词的双语例句
     Genotyping analysis of multiple loci in single cell by primer extension preamplification and degenerate oligonucleotide primed-PCR
     PEP、DOP-PCR单细胞多基因位点检测研究
短句来源
     We have established a method of primer extension preamplification (PEP) method and studied its practicability 15 oligonucleotides were selected as PEP primer and was amplified under low strength,followed by specifically amplification with loci specific primers Through PEP PCR,about 75% of whole human genomic DNA could be obtained.
     提高微量检材的利用率 ,建立PEP方法并对其法医学应用进行可行性研究。 用 15个随机寡核苷酸的序列作为引物 ,低特异性下扩增出微量DNA ,以此扩增物作为模扳 ,进行特异基因座扩增。
短句来源
     Methods:Whole genome of single cell were amplified using 15-base random primers (Primer extension preamplification,PEP),then a small aliquot of PEP product were analyzed by locus-specific nest PCR amplification. The procedure was e-valuated by the detection of dystrophin exons 8,17,19,44,45,48 and human testis-determining gene or detecting (SRY)in single lymphocyte from known sources and single blastomer from the couples with no family history of DMD.
     方法:获取单个正常男女淋巴细胞和无杜氏肌营养不良(DMD)家族史的单个卵裂球,15碱基随机引物PEP扩增单细胞全基因组,再以巢式PCR技术检测PEP反应产物中DMD基因外显子8,17,19,44,45,48及Y染色体上的睾丸决定基因SRY。
短句来源
     Plasma DNA in blood samples of 65 pregnant women with the gestational period of 5~40 weeks were extracted and primer extension preamplification (PEP) and nested polymerase chain reaction were employed to amplify the SRY gene.
     (2 )提取 65例孕妇 (5~ 40孕周 )外周血血浆DNA。
短句来源
     Primer extension preamplification(PEP) and ploymerase chain reaction(PCR) based on single cell were adopted to detect SRY gene. Fluorescence in situ hybridization was also applied.
     采用单细胞PEP-PCR技术检测SRY基因,并进行荧光原位杂交分析。
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  primer extension preamplification
Primer Extension Preamplification (PEP) methods that can amplify the entire genome 100-fold or more offer a potential solution to this problem.
      
Primer extension preamplification (PEP) methods that can amplify the entire genome 100-fold or more, offer a potential solution to this problem.
      
A recently developed method, primer extension preamplification (PEP), was used to amplify the bovine sperm genome prior to amplification of specific loci by the polymerase chain reaction (PCR).
      
The entire genome of a single NRBC was amplified by primer extension preamplification (PEP).
      
Sex was determined after primer extension preamplification (PEP) of the entire genome of a single cell, and human leukocyte antigen (HLA)-DQ α type was determined after further amplification of this gene.
      
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Haplotype of porcine single sperm was analyzed by PCR method through using primer extension preamplification and heminesting primer design strategy. The PCR products were run on 8% polyacrylamide gel, and visualized by silver staining method.The results showed that the haplotypes of individual sperm can be unequivocally demonstrated by these methods. The application of single sperm typing provides a unique tool for the construction of high resolution genetic map and the study of genetic phenomena...

Haplotype of porcine single sperm was analyzed by PCR method through using primer extension preamplification and heminesting primer design strategy. The PCR products were run on 8% polyacrylamide gel, and visualized by silver staining method.The results showed that the haplotypes of individual sperm can be unequivocally demonstrated by these methods. The application of single sperm typing provides a unique tool for the construction of high resolution genetic map and the study of genetic phenomena requiring large sample size.

应用引物延伸预扩增、内嵌引物设计策略进行PCR扩增并结合聚丙烯酰胺凝胶电泳及银染技术,对猪单个精子12号染色体上4个微卫星位点进行了PCR扩增。结果表明,上述技术的应用可以清晰地鉴定出每个精子的单倍型,单精子分型技术的应用为猪高精度遗传作图及需要样本量足够大的遗传现象研究提供了独特的工具。

In oder to study the feasibility of prenatal genetic analysis of single fetal nucleated red blood cell (NRBC) from maternal blood, peripheral blood from 45 pregnant women was subjected to discontinuous density gradient centrifugation to obtain single NRBC by using a micromanipulator. Primer extension preamplification (PEP) and polymerase chain reaction (PCR) of a Y chromosome specific repeat sequence were performed in 24 patients (group A). Only PCR of a Y chromosome specific repeat sequence was performed...

In oder to study the feasibility of prenatal genetic analysis of single fetal nucleated red blood cell (NRBC) from maternal blood, peripheral blood from 45 pregnant women was subjected to discontinuous density gradient centrifugation to obtain single NRBC by using a micromanipulator. Primer extension preamplification (PEP) and polymerase chain reaction (PCR) of a Y chromosome specific repeat sequence were performed in 24 patients (group A). Only PCR of a Y chromosome specific repeat sequence was performed in the remaining 21 cases (group B). It was found that NRBCs were detectable in 24 out of 45 pregnant women. In group A, male pregnancy was correctly predicted in 70 %. The overall agreement of the diagnosis was 83 3 %. In group B, the Y specific DNA sequence was not amplified. It was suggested that the prenatal genetic analysis of single fetal NRBC by using PEP PCR method is feasible.

为探讨母血中单个胎儿有核红细胞 (NRBC)行产前基因分析的可行性 ,对 45名孕妇外周血行不连续密度梯度离心 ,显微操作获取单个 NRBC,A组 2 4例进行引物延伸预扩增 (PEP) ,后行 Y染色体特异性 DYZ1基因的聚合酶链反应 (PCR) ,B组 2 1例直接进行 Y染色体特异性 DYZ1基因的 PCR。发现 45名孕妇中有 2 4名检出 NRBC,A组中男胎符合率为 70 % ,总符合率为 83.3% ,B组则均未扩增到 PCR产物。提示用母血中单个胎儿 NRBC经 PEP- PCR法行产前基因分析是可行的。

We have established a method of primer extension preamplification (PEP) method and studied its practicability 15 oligonucleotides were selected as PEP primer and was amplified under low strength,followed by specifically amplification with loci specific primers Through PEP PCR,about 75% of whole human genomic DNA could be obtained. The results of PCR analyses of 20 loci from PEP products were consistent with those of PCR analyses from the original DNA samples Good results can be obtained...

We have established a method of primer extension preamplification (PEP) method and studied its practicability 15 oligonucleotides were selected as PEP primer and was amplified under low strength,followed by specifically amplification with loci specific primers Through PEP PCR,about 75% of whole human genomic DNA could be obtained. The results of PCR analyses of 20 loci from PEP products were consistent with those of PCR analyses from the original DNA samples Good results can be obtained from 1ng template DNA It showed that the method of PEP PCR can be applied in the forensic science practice It is important for the increasing usage of the trace evidence and providing nuclei genetic information by PEP PCR method

提高微量检材的利用率 ,建立PEP方法并对其法医学应用进行可行性研究。用 15个随机寡核苷酸的序列作为引物 ,低特异性下扩增出微量DNA ,以此扩增物作为模扳 ,进行特异基因座扩增。经PMCT118,GABAR ,DYS19,D18S849,D3S1744,D12S10 90 ,D8S1179,D2 1S11,D18S5 1,D5S818,D13S3 17,D7S82 0 ,D3S13 5 8,vWA ,FGA ,TH0 1,CSF1PO ,TPOX ,D16S5 3 9及Amelogene等 2 0个基因座的PEP PCR与直接的PCR分析比较 ,二者分型结果一致 ,即使 1ngDNA也能得到正确分型。PEP方法可用于法医学检验 ,有利于微量物证的利用 ,使物证检材提供尽可能多的信息

 
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