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bt toxin protein     
相关语句
  bt毒蛋白
     Transgenic Corn with Bt Toxin Protein Gene and Insect-resistance
     转Bt毒蛋白基因玉米及其抗虫性研究进展
短句来源
     With Bt transgenic cotton Kemian 1 as the testing material, the content of free amino acid , soluble protein and Bt toxin protein in fruit branch leaves were studied under the heat stress (40 ℃) which lasted for 48 h.
     以科棉1号为试验材料,研究连续48 h的40℃高温胁迫对棉花果枝叶片游离氨基酸、可溶性蛋白和Bt毒蛋白含量的影响。
短句来源
     Bt toxin protein determine the nature to soil at present and for analytic approach of a trace method that ration measure (Western-blotting), SDS-PAGE law, spot trace enzyme unite the immune adsorption method (dot-blot ELISA), flowing type cell’s appearance law (FCM), ELISA dull and stereotyped reagent box and reagent article and living beings determine the law.
     目前对土壤中Bt毒蛋白定性和定量检测的方法主要有印迹分析法(Western-blotting)、SDS-PAGE法、斑点印迹酶联免疫吸附法(dot-blotELISA)、流式细胞仪法(Flowcytometer,FCM)、ELISA平板试剂盒及试剂条和生物测定法。
短句来源
     In recent years significant progress has been made on Bt genetics and molecular biology, especially the Bt toxin protein gene in engineered bacterium.
     近年来,有关Bt毒蛋白基因遗传学和分子生物学的研究已取得显著进展,特别是用该基因构建工程菌成为研究的热点。
短句来源
     But in a word,if plant and transfer to the gene crop of Bt for a long time, will possibly cause the accumulation in the soil of Bt toxin protein, and threaten the balance of the whole soil ecosystem finally.
     但是总之,如果长期种植转Bt基因作物,很可能会造成Bt毒蛋白在土壤中的积累,并最终威胁到整个土壤生态系统的平衡。
短句来源
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  bt杀虫蛋白
     Comparison among Three ELISA Methods for Bt Toxin Protein
     3种ELISA法检测Bt杀虫蛋白的比较研究
短句来源
     The antigen and antiserum of crystal toxin protein of Bacillus thuringiensis srtains HD-1 and HD-68 which were soluble in alkali were prepared. A Dot-ELISA for Bt toxin protein had been developed. The detectable level of Bt toxin protein by Dot-ELISA was 87. 5ng/ml.
     用苏云金芽孢杆菌HD-1和HD-68晶体杀虫蛋白的碱溶产物作为抗原,制备相应的 抗血清,建立了检测 Bt杀虫蛋白的 Dot-ELISA,可检测 Bt杀虫蛋白的最低水平为 87.5ng/ml。
短句来源
     The results showed that the two assays could be used for detecting Bt toxin protein in transgenic cotton, but some problems (such as interference of sample green color) need to be solved.
     通过检测方法研究,明确了影响检测的一些因素并初步建立起了检测棉叶样本中Bt杀虫蛋白的相关技术。
短句来源
     The results indicated that the time point of when the expression of Bt toxin protein in transgenic insect-resistant cotton plants became lower during the development was affected because different thermal treatments had great impact on the development of transgenic plants and their Bt toxin gene expression level.
     结果表明,温度变化影响抗虫棉早期的生长发育和Bt杀虫基因的表达,从而影响了抗虫棉生长发育过程中Bt杀虫蛋白含量降低的时间。
短句来源
     Three ELISA methods were experimented to assay purified Bt toxin protein. The rested result indicated that the minimal detectable value from both direct ELISA and double antibody sandwich ELISA was 0.94 ng /well. But there appeared very significant linear correlation between quantity of Bt toxin protein and their OD value in direct ELISA and there exited significant linear correlation between quantity of Bt toxin protein and in double antibody sandwich ELISA.
     运用3种ELISA法对提纯的Bt杀虫蛋白进行检测,结果表明:直接法ELISA和夹心法ELISA的灵敏度相同,都为0.94 ng/孔,但直接法ELISA中Bt蛋白量与OD值间呈极显著的线性关系,夹心法ELISA中Bt蛋白量与OD值间呈显著的线性关系;
短句来源
  bt毒素
     And,as the development of detecting techniques for Bt toxin protein and soil were made by oversea researchers.
     加之Bt毒素蛋白检测技术的发展 ,国外学者们围绕纯化毒素蛋白与土壤的相互关系等方面开展了大量研究 ,并取得了一些结果 .
短句来源
     The soil is a main place that the material circulation and energy transform course in the ecosystem, transfer to Bt other source Bt toxin protein of gene expression, gene of plant can enter the soil ecosystem through plant incomplete body, root, root exudates and way of disseminating etc. of pollen, once Bt toxin protein that height take specially the accumulate among soil, cause soil peculiar biological function group and soil variety change, even produce the effect of one grade of antithetical couplets.
     土壤是生态系统中物质循环和能量转化过程的主要场所,转Bt基因植物的外源基因表达的Bt毒蛋白可以通过植株残体、根及根系分泌物和花粉的散播等途径进入土壤生态系统,这些高度特化了的Bt毒素蛋白一旦在土壤中积累,将会导致土壤特异生物功能类群以及土壤多样性发生改变,甚至产生级联效应。
短句来源
  bt蛋白
     Improved histochemical staining for GUS activity,PCR and Western blotting were used to detect the population of Bt rice crossed to conventional rice varieties A total of 392 plants expressing Bt toxin protein were found in 394 GUS positive plants.
     利用PCR、GUS染色和Western印迹杂交技术检测了Bt水稻杂交后代群体,发现在394株GUS阳性株中,共有392株表达Bt蛋白,协同表达株率达99.49%。
短句来源
     Improved histochemical staining for GUS activity, PCR and Western blotting were used to analyse the progeny population of Bt rice crossed with conventional rice varieties. A total of 392 plants expressing Bt toxin protein were found in 394 GUS postive plants.
     用PCR、GUS染色和Western点杂交技术检测了Bt水稻杂交后代群体,在394株GUS阳性 株中,有392株表达Bt蛋白,协同表达株率达99.49%。
短句来源
     Three ELISA methods were experimented to assay purified Bt toxin protein. The rested result indicated that the minimal detectable value from both direct ELISA and double antibody sandwich ELISA was 0.94 ng /well. But there appeared very significant linear correlation between quantity of Bt toxin protein and their OD value in direct ELISA and there exited significant linear correlation between quantity of Bt toxin protein and in double antibody sandwich ELISA.
     运用3种ELISA法对提纯的Bt杀虫蛋白进行检测,结果表明:直接法ELISA和夹心法ELISA的灵敏度相同,都为0.94 ng/孔,但直接法ELISA中Bt蛋白量与OD值间呈极显著的线性关系,夹心法ELISA中Bt蛋白量与OD值间呈显著的线性关系;
短句来源
     The minimal detectable value by indirect ELISA was lower. When HRP-IgG was used,the minimal detectable value was 15 ng/well and there appeared significant linear correlation between quantity of Bt toxin protein and their OD value. When AKP-IgG was used,the minimal detectable value was 3.75 ng/well and there appeared very significant linear correlation between quantity of Bt toxin protein and their OD value.
     间接法ELISA的灵敏度较前两者低,使用HRP-IgG的灵敏度为15 ng/孔,Bt蛋白量与OD值间呈显著的线性关系,使用AKP-IgG的灵敏度为3.75ng/孔,Bt蛋白量与OD值间呈极显著的线性关系。
短句来源

 

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      bt toxin protein
    Double-antibody sandwich ELISA analysis revealed Bt toxin protein expression in the transgenic plants.
          


    In this study,an insecticidal crystal protein gene cryIA(c)of Bacillus thuringiensis via the broad host range plasmids pUCP18 and pUCP19 was transformed into biocontrol bacterium Pseudomonas fluorescens P303 by electroporation.southern blotting and Western blotting assays respectively verified the existence of cryIA(c)gene and expression of Bt toxin protein in the transformation.Results of bioassay showed that these new genetically engineered strains were not only inhibitory to the plant Pathogen...

    In this study,an insecticidal crystal protein gene cryIA(c)of Bacillus thuringiensis via the broad host range plasmids pUCP18 and pUCP19 was transformed into biocontrol bacterium Pseudomonas fluorescens P303 by electroporation.southern blotting and Western blotting assays respectively verified the existence of cryIA(c)gene and expression of Bt toxin protein in the transformation.Results of bioassay showed that these new genetically engineered strains were not only inhibitory to the plant Pathogen Geumannomyces graminis var tritici, but also insecticidal to the corn borer and diamond back moth.

    以广寄主范围质粒pUCP18和pUCP19为载体,用电激转化法,将苏云金杆菌杀虫蛋白基因crylA(c)片段导入了荧光假单胞菌P303菌株。Southern印迹分析和Western印迹分析分别证实crylA(c)基因的导入和晶体蛋白在P303中的表达。生物活性测定结果表明,新构建的荧光假单胞工程菌株不仅保持了野生型自然菌株对小麦全蚀病有良好的抑菌活性,而且表现出对小菜蛾、玉米螟有显著的毒杀作用。

    Maize embryogenic calli were bombarded by high- velocity microprojectiles coated withplasmid pB48.4l5 DNA, which contained Bt-toxin protein gene and hygromycin phosphotransferase gene.After being selected on medium with hygromycin the resistant embryogenic calli were transferred to regen-eration medium on which embroids formed and germinated.The results of dot blotting and Southern hy-bridization certified the integration of Bt-gene into maize genome. The prelirninary results of insect...

    Maize embryogenic calli were bombarded by high- velocity microprojectiles coated withplasmid pB48.4l5 DNA, which contained Bt-toxin protein gene and hygromycin phosphotransferase gene.After being selected on medium with hygromycin the resistant embryogenic calli were transferred to regen-eration medium on which embroids formed and germinated.The results of dot blotting and Southern hy-bridization certified the integration of Bt-gene into maize genome. The prelirninary results of insect resis-tance evaluation on the progenies from one Bt-transgenic plant showed that the ratio of resistant and sus-ceptible plants was near to l:l.

    用基因枪直接轰击玉米胚性愈伤组织,经潮霉素抗性选择平均每皿可得到0.7~2.0块抗性愈伤组织,且大部分可再生植株。对这些从抗性愈伤组织分化的植株进行点杂交和Southern吸印杂交的结果也证明Bt基因已插入玉米染色体组。对一个转Bt基因植株的测交后代进行了玉米螟抗性的田间鉴定,抗虫和感虫植株的比例接近1:1,符合孟德尔单显性基因的遗传规律。

    Studies on maize transformation have been carried out through bombarding by particle gun, ultrasonicating in a DNA buffer or ovary injecting by a self-made mi-croinjector. The plasmid pB48.415, which carries a 3'-end truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation. Transgenic maize plants were obtained from immature embryos and embryogenic calli bombarded with particle gun, embryogenic calli ultrasonicated under different conditions...

    Studies on maize transformation have been carried out through bombarding by particle gun, ultrasonicating in a DNA buffer or ovary injecting by a self-made mi-croinjector. The plasmid pB48.415, which carries a 3'-end truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation. Transgenic maize plants were obtained from immature embryos and embryogenic calli bombarded with particle gun, embryogenic calli ultrasonicated under different conditions or ovaries injected after 10-20h of pollination. The results of Dot blotting and Southern blotting analysis certified the integration of Bt gene into maize genome.

    用基因枪、超声波和子房注射法转化玉米,所用质粒pB48.415带有3'端截短的Bt毒蛋白基因和潮霉素磷酸转移酶(hpt)基因。用基因枪轰击玉米胚性愈伤组织和幼胚,超声波处理玉米胚性愈伤组织,用自制的微玻针注射授粉后10~20h的玉米子房,均已成功地获得了转Bt基因的玉米植株。点杂交和Southern吸印杂交的结果都证明在转基因玉米植株中存在Bt毒蛋白基因。

     
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