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  chenopodium
This review summarizes the long-term research of photoperiodic flower induction in two Chenopodium species, one of which, C.
      
Flowering of Cultivated Green and SAN 9789-Treated Chenopodium rubrum Plants Exposed to White, Blue, and Red Light
      
Temporal dynamics of soil nematode community structure under invasive Ambrosia trifida and native Chenopodium serotinum
      
Temporal dynamics of soil nematode community structure at the depth of 0 - 30 cm was compared under invasive Ambrosia trifida and native Chenopodium serotinum in an abandoned cropland in Northeast China.
      
Gynomonoecy in Chenopodium quinoa (Chenopodiaceae): variation in inflorescence and floral types in some accessions
      
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A virus was isolated from the orchid Cattleya in Hainan,through sap inoculiation on Chenopodium amaranticolor, an indicator plant,Single lesion inoculation was made on Tetragonia expansa more than three times,Thevirus was purified with the procedure including PEG precipitation and diff-erential centrifugation. Its rod-shape particles could be specifically decor-dted in IEM test by the antiserum against Odontoglossum ringspot virus(ORSV), ELISA and Ouchterlony double diffusion assay reveal that thevirus...

A virus was isolated from the orchid Cattleya in Hainan,through sap inoculiation on Chenopodium amaranticolor, an indicator plant,Single lesion inoculation was made on Tetragonia expansa more than three times,Thevirus was purified with the procedure including PEG precipitation and diff-erential centrifugation. Its rod-shape particles could be specifically decor-dted in IEM test by the antiserum against Odontoglossum ringspot virus(ORSV), ELISA and Ouchterlony double diffusion assay reveal that thevirus isolated from the orchid was serologically closely related to ORSVand homologously partially related to Tobacco mosaic virus(TMV),SDS PAGE shows that the coat protein of the virion consisted of only one sub-unit with the molecular weight of 17.3KD,and nucleic acid electrophoresison agarose gel displayed one band which possessed similar molecular weightto the RNA of TMy. These biological,seroiogical and biochemical propertiesof the virus isolated suggested that it should be ORSV belonging to Toba-moviruses. ORSV has been one of the two most prevalent viruses infecting nearly all kinds of orchid cultivars and vanilla worldwide. For the purposeof providing a virus detection method for orchid growers,we prepared a specific antiserum by immunizing rabbits with the purified virus and madedetection on some diseased orchids by using SPA ELISA.

从叶片表现褪绿、褐色坏死斑的卡特兰病株中获得一个病毒分离物,利用番杏进行多次单斑分离纯化,并用其做繁殖寄主,来用PEG沉淀和差速离心等方法获得提纯病毒。该病毒分离物提纯液具有典型的核蛋白紫外吸收特征,电镇观察病毒粒体呈直杆状,可被齿兰环斑病毒抗血清特异装饰,琼脂免疫双扩散和ELISA实验表明,读分离物和ORSV血清学关系密切,和TMV有部分同源关系,SDS—PAGE表明病毒外壳蛋白由一种分子量为17.3KD亚基构成,琼脂糖电泳显示单—RNA组份。根据鉴别寄主症状、位体形态组成等理化特征,以及血清学特性,鉴定该分离物属于烟草花叶病毒组,和齿兰环斑病毒已报导的一些分离物比较接近。用纯化病毒免疫家兔,成功地制备出特异性抗血清,并用SPA—ELISA方法对一些兰花病株进行了检测研究。

Citrus tatter leaf virus(CTLV)was mechanically transmitted to 23 herbaceousspecies from eight families. It induced local lesion on Vigna sinensis, systemic yellow spotand distortion on Chenopodium quinoa and C. amaranticolor. local spot or Celosia cristataand systemic light mottle on Nicotiana celevilandii. It was also back transmitted fromN. celevilandii to Citrus reficulata,C, citrange and caused typical "tatter leaf "symptoms.DEP for this virus was 10-5, when tested from infected leaf sap of C. reticulata....

Citrus tatter leaf virus(CTLV)was mechanically transmitted to 23 herbaceousspecies from eight families. It induced local lesion on Vigna sinensis, systemic yellow spotand distortion on Chenopodium quinoa and C. amaranticolor. local spot or Celosia cristataand systemic light mottle on Nicotiana celevilandii. It was also back transmitted fromN. celevilandii to Citrus reficulata,C, citrange and caused typical "tatter leaf "symptoms.DEP for this virus was 10-5, when tested from infected leaf sap of C. reticulata. Virusparticles that characterized as Capiloviruses were detected under electron microscopefrom leaf-sap of diseased hosts above. Herbaceous species can be used as indicators forthis virus. Virus crystallized bodies were found in phloem cells of C. reticulata anc C.citrange infected with CTLV, the same structures were also observed in mesophyll cellsof Chenopodium quinoa and N. clevilandii infected with CTLV. but the crystallizationbodies were looser aggregated and smaller. Degradation of reticulum and accumulating ofstarch bodies was observed on chloroplasts of infected mesophyll cells of above hosts.This is the first report of cytopathological characteristics of this virus disease. Positiveserological relationship was found between CTLV and apple stem grooving virus( ASGV) by agar double diffusion test and ISEM. This indicated the possibility for usingASGV antibodies to detect CTLV in plant materials.

用汁液摩擦接种方法对柑桔碎叶病毒(Citrus tatter leaf virus,CTLV)进行了进一步的生物学鉴定.结果表明,该病毒除在豇豆上引起枯斑外,还侵染克里芙兰烟(Nicotianaclevilandii)产生系统斑驳和轻花叶,侵染昆诺藜(Chenopodium quinoa)和苋色藜(C.amaranticolor)引起系统坏死和花叶,侵染鸡冠花(Celosia cristata)引起局部环斑.这些草本寄主均可作为CTLV的指示植物.在发病的柑桔叶汁液中,测得CTLV的稀释限点为10~(-5).经汁液摩擦接种,可以将CTLV从克里芙兰烟传播到木本指示植物柑桔和枳橙上.感病植物材料的组织超微结构观察结果表明:CTLV感染柑桔、枳橙、克里芙兰烟和昆诺藜均使其叶肉薄壁细胞的叶绿体出现淀粉沉积、叶绿体片层结构解体和消失;病毒在受感染的植株叶脉韧皮部细胞中紧密聚集,形成病毒结晶体;这种结构也出现在叶肉薄壁细胞中.这是关于该病毒组织病变的首次报道.用直接负染方法从感染CTLV的柑桔(枳橙)、克里芙兰烟和昆诺藜病叶中均能检查到线状病毒粒子,用改良的Deriick’s免疫电镜方法和琼脂双扩散方法测定均显示该病毒...

用汁液摩擦接种方法对柑桔碎叶病毒(Citrus tatter leaf virus,CTLV)进行了进一步的生物学鉴定.结果表明,该病毒除在豇豆上引起枯斑外,还侵染克里芙兰烟(Nicotianaclevilandii)产生系统斑驳和轻花叶,侵染昆诺藜(Chenopodium quinoa)和苋色藜(C.amaranticolor)引起系统坏死和花叶,侵染鸡冠花(Celosia cristata)引起局部环斑.这些草本寄主均可作为CTLV的指示植物.在发病的柑桔叶汁液中,测得CTLV的稀释限点为10~(-5).经汁液摩擦接种,可以将CTLV从克里芙兰烟传播到木本指示植物柑桔和枳橙上.感病植物材料的组织超微结构观察结果表明:CTLV感染柑桔、枳橙、克里芙兰烟和昆诺藜均使其叶肉薄壁细胞的叶绿体出现淀粉沉积、叶绿体片层结构解体和消失;病毒在受感染的植株叶脉韧皮部细胞中紧密聚集,形成病毒结晶体;这种结构也出现在叶肉薄壁细胞中.这是关于该病毒组织病变的首次报道.用直接负染方法从感染CTLV的柑桔(枳橙)、克里芙兰烟和昆诺藜病叶中均能检查到线状病毒粒子,用改良的Deriick’s免疫电镜方法和琼脂双扩散方法测定均显示该病毒与苹果茎沟病毒(Apple stem grooving virus,ASGV)有强阳性反应,用ASGV抗血清可以进行植物材料中CTLV的检测.

Two virus, isolates,P-935 and B-934 ,were obtained from diseased Pisum sativum and Vicia faba,and 14 plant species in 6 families were mechanically inoculated by the isolates.Among the tested plants,P-935 could infect 13 species and B-934 infected 14 species. The two isolates pro-duced similar symptoms on these plants,except for symptoms on Phaseolus vulgaris.The two Iso-lates were easily transmitted by Myzus persicae in a nonpersistent manner.P-935 and B-934 were purified form Chenopodium quinoa by a method...

Two virus, isolates,P-935 and B-934 ,were obtained from diseased Pisum sativum and Vicia faba,and 14 plant species in 6 families were mechanically inoculated by the isolates.Among the tested plants,P-935 could infect 13 species and B-934 infected 14 species. The two isolates pro-duced similar symptoms on these plants,except for symptoms on Phaseolus vulgaris.The two Iso-lates were easily transmitted by Myzus persicae in a nonpersistent manner.P-935 and B-934 were purified form Chenopodium quinoa by a method that yielded up to 147 mg/kg and 1 28 mg/kg tis-sue,respectively. Purified preparation of two isolates contained isometric particles ca. 25 nm in di-ameter,The capsid protein contained two polypeptides with molecular weight of 42.5 kD and 21.2 kD. Amorphous inclusions with membrane accumulations were found in the cytoplasm of infected Pisum sativum leaf tissue. In agarose gel diffusion tests,P-935 and B-934 strongly reacted with the antisera of BBWV serotype Ⅱ.On the bases of these characteristics,P-935 and B-934 were identi-fied as broad bean wilt virus(serotype Ⅱ).Field survey in Hangzhou surburb showed viral dis-eases were severe in Pisum sativum and Vicia faba.

从浙江省豌豆和蚕豆病株上获得两个病毒分离物,编号为P-935和B-934.人工摩擦接种6科19种植物,P-935能侵染4科13种植物,B-934能侵染4科14种植物,除菜豆外,它们在这些植物上的症状十分相似。P-935和B-934的钝化温度为50-55℃,稀释限点为10 ̄(-3)-10 ̄(-4),体外保毒期分别为5天和4天。两个分离物均能由桃蚜以非持久性方式传毒,用昆诺藜作繁殖寄主均提取到了大量病毒粒子,提纯病毒粒子球形,平均直径25nm。病毒外壳蛋白均由两种亚基组成,分子量分别为42.5kD和21.2kD,感病叶片组织中观察到了无定形的泡状内含体,在琼脂双扩散试验中,P-935和B-934均能与蚕豆萎蔫病毒(BBWV)抗血清(血清型Ⅱ)形成清晰沉淀线。根据以上特性,P-935和B-934均被鉴定为蚕豆萎蔫病毒,且都属血清型Ⅱ.初步调查表明杭州郊区豌豆病毒病均由BBWV引起,蚕豆病毒病主要有BBWV和黄瓜花叶病毒(CMV)。

 
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