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   cute hepatitis 在 消化系统疾病 分类中 的翻译结果: 查询用时:0.545秒
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Cytomegalovirus(CMV) DNA in serum samples of 159 patients with hepatitis B was detected by polymerase chain reaction(PCR).Simultaneously,CMVIgM was also determined by enzyme linked immunosorbent assay(ELISA)Positive CMVDNA and(or) CMVIgM were used as diagnostic criteria for active CMV infectionResults:the positive rates of CMVDNA and CMVIgM were 3019% and 1447% respectivelyThe rate of active infection for these patients was 3370%,which was significantly higher than that(543%) of the healthy controlsAmong...

Cytomegalovirus(CMV) DNA in serum samples of 159 patients with hepatitis B was detected by polymerase chain reaction(PCR).Simultaneously,CMVIgM was also determined by enzyme linked immunosorbent assay(ELISA)Positive CMVDNA and(or) CMVIgM were used as diagnostic criteria for active CMV infectionResults:the positive rates of CMVDNA and CMVIgM were 3019% and 1447% respectivelyThe rate of active infection for these patients was 3370%,which was significantly higher than that(543%) of the healthy controlsAmong these 159 patients,the active infection rates of cute hepatitis,mild chronic hepatitis,moderate chronic hepatitis,severe chronic hepatitis and liver cirrhosis were 1786%,1667%,4286%,5000% and 3478% respectivelyIn the peripheral blood of patients with active infection,the percentage of CD4 was significantly lower,and the persentage of CD8 higher than those of the controlsHence,the ratio of CD4/CD8 was significantly lowered in active CMV infectionOur results suggest that the sensitivity of PCR is higher than ELISAAnd early,prompt and correct diagnosis of active CMV infection of patients with hepatitis B can be achieved by the combined use of these two assaysThe superinfection of CMV and HBV may futher aggravate the impairment of the cellular immune function,worsen the disease and prolong its course

应用聚合酶链反应(PCR)技术检测159例乙型肝炎患者血清中巨细胞病毒脱氧核糖核酸(CMV-DNA),结合酶联免疫吸附法(ELISA)检测血清中CMV-IgM,以CMV-DNA和(或)CMV-IgM阳性为活动性CMV感染诊断标志。结果显示:CMV-DNA和CMV-IgM阳性率分别为30.19%、14.47%。乙肝患者活动性CMV感染率为33.70%,明显高于健康对照5.43%。其中急性肝炎、慢性轻度肝炎、慢性中度肝炎、慢性重度肝炎及肝硬化感染率分别为17.86%、16.67%、42.86%、50.00%、34.78%。与对照组相比,合并活动性CMV感染的乙肝患者外周血CD4百分率降低而CD8百分率升高,导致CD4/CD8比值明显下降。提示:PCR技术敏感性明显高于ELISA法,两法联合使用可早期、快速、准确诊断乙肝患者活动性CMV感染。CMV与HBV重合感染可进一步抑制乙肝患者机体细胞免疫功能,使患者病情加重或迁延不愈。

Aim:This study was aimed at investigating the epidemiologic and clinical characteristics. Methods:28 cases wsa hospitalized in our hospital of the 28 patients 16 were male and 12 female, aged 23--69 years. Of them, 8 were acute hepatitis, 4 were mild (CH), 4 moderate (CH), 3 chronic severe hepatitis, 9 active hepatocirrhosis. HGV RNA was detected by RT-PCR. Result; 15 patients had received blood or plasma transfusion (53. 6%), 1 was an intravenous drug abuser (3. 6% ). The other 12 had no direct...

Aim:This study was aimed at investigating the epidemiologic and clinical characteristics. Methods:28 cases wsa hospitalized in our hospital of the 28 patients 16 were male and 12 female, aged 23--69 years. Of them, 8 were acute hepatitis, 4 were mild (CH), 4 moderate (CH), 3 chronic severe hepatitis, 9 active hepatocirrhosis. HGV RNA was detected by RT-PCR. Result; 15 patients had received blood or plasma transfusion (53. 6%), 1 was an intravenous drug abuser (3. 6% ). The other 12 had no direct cause (42. 6% ). The main clinical manifestation was loss of appetite (100%), fatigue (92. 9%), greenish -brown colour urine (75% ) , nausea (53. 6%). Virus marks tests showed 4patients were infected HGV only. 13 HBV coinfection HGV, 6 HCV coinfection HGV, 3 HCV, HBV, HGV coinfection. Sera tests of hepato-function showed: TBil 45. 08±22.48μmol/L, ALT 318. 64±359. 95U/L Sera tests of fibrosis marks showed: HA 515. 39±800. 79μg/L,LN 323±578. 13μg/L,PCⅡ151. 71±47. 34μ/L,CLIV 67. 35±15. 27ng/L. Conclusion: ①HGV infections is a parenteral transmission route of viruses (blood transfusion) . ② HGV infection can cause a-cute hepatitis and develop a chronic course. ③ HGV infection occurs frequently in coinfection with HBV or/and HCV. ④ Hepatitis G seems to have a mild course as ALT values are concerned.

目的:调查庚型肝炎的流行病学及临床特点。方法:28例患者系我院住院病人,男16例,女12例,年龄23~69岁,其中急性肝炎8例,慢性肝炎轻度4例,慢性肝炎中度4例,慢性重型肝炎3例,活动性肝硬变9例;检测患者HGV-RNA采用RT-PCR法。结果:28例患者中有输血史者15例(53.6%),有静脉注射毒品史者1例(3.6%),不明原因者12例(42.6%);临床症状以纳差(100%),乏力(92.9%),尿色加深(75%)及恶心(53.6%)为主;病毒标志物检洲单纯庚型肝炎病毒感染4例(14.3%),乙、庚重叠感染13例(46.4%),丙、庚重叠感染6例(21.4%),乙、丙、庚重叠感染3例(10.7%);肝功能检测:TBil:45.08±22.48μmol/L,ALT 318.64±359.95U/L;纤维化指标:HA 515.39±800.79μg/L,LN 323±578.13μg/L,PCⅢ151.71±47.34μg/L,C Ⅳ 67.35±15.27ng//L。结论:①HGV主要经输血途径传播。同时存在其他肠道外途径传播如静脉注射毒品等。②HGV可引起急性、慢性、重型肝炎、肝硬变,易复发,易致慢性...

目的:调查庚型肝炎的流行病学及临床特点。方法:28例患者系我院住院病人,男16例,女12例,年龄23~69岁,其中急性肝炎8例,慢性肝炎轻度4例,慢性肝炎中度4例,慢性重型肝炎3例,活动性肝硬变9例;检测患者HGV-RNA采用RT-PCR法。结果:28例患者中有输血史者15例(53.6%),有静脉注射毒品史者1例(3.6%),不明原因者12例(42.6%);临床症状以纳差(100%),乏力(92.9%),尿色加深(75%)及恶心(53.6%)为主;病毒标志物检洲单纯庚型肝炎病毒感染4例(14.3%),乙、庚重叠感染13例(46.4%),丙、庚重叠感染6例(21.4%),乙、丙、庚重叠感染3例(10.7%);肝功能检测:TBil:45.08±22.48μmol/L,ALT 318.64±359.95U/L;纤维化指标:HA 515.39±800.79μg/L,LN 323±578.13μg/L,PCⅢ151.71±47.34μg/L,C Ⅳ 67.35±15.27ng//L。结论:①HGV主要经输血途径传播。同时存在其他肠道外途径传播如静脉注射毒品等。②HGV可引起急性、慢性、重型肝炎、肝硬变,易复发,易致慢性化。③HGV可与HGV和/或HCV同时或重叠感染。④HGV一般临床症状及肝功能损害较轻。

Objective: To study the changes of granulocyte - macrophage colony - stimulating factor (GM-CSF), the count of write blood cells and HBV DNA of Pallets with different type of hepatitis B. Methods: Sera obtained from 60 patients in 4 groups respectively with a-cute hepatitis B, chronic hepatitis B, gravity hepatitis B and posthepatitis cirrhosis were examined using GM-CSF kit. The count of WBC and HBV DNA were detected by rutine. 15 healthy donors were used as control. Results: Compared with...

Objective: To study the changes of granulocyte - macrophage colony - stimulating factor (GM-CSF), the count of write blood cells and HBV DNA of Pallets with different type of hepatitis B. Methods: Sera obtained from 60 patients in 4 groups respectively with a-cute hepatitis B, chronic hepatitis B, gravity hepatitis B and posthepatitis cirrhosis were examined using GM-CSF kit. The count of WBC and HBV DNA were detected by rutine. 15 healthy donors were used as control. Results: Compared with healthy, HBV DNA level was medium, the concentration of GM-CSF and WBC had no changes in acute hepatitis; HBV DNA was high, the concentration of GM-CSF and WBC decreased obviously in chronic hepatitis and posthepatitis cirrhosis; HBV DNA was high, the concentration of GM - CSF decreased obviously, the level of WBC increased obviously in gravity hepatitis B. The concentration of GM - CSF was parallel to the level of WBC but to HBV DNA. Conclusions: The concentration of GM-CSF can not only affect the count of WBC but also resist the virus.

目的:探讨血清粒细胞-巨噬细胞集落刺激因子(GM-CSF)对乙型肝炎患者的临床意义。方法:采用放射免疫法检测各型乙型肝炎和肝炎后肝硬化患者与健康受试者血清GM-CSF浓度,常规检测外周血白细胞(WBC)计数,聚合酶链反应(PCR)法检测外周血乙肝病毒基因组(HBV DNA)含量。结果:与正常人相比,慢性肝炎、肝炎后肝硬化患者血清GM-CSF水平、WBC水平下降(P<0.05);重型肝炎患者血清GM-CSF水平显著下降(P<0.01),WBC水平显著升高(P<0.01)。HBV DNA含量在急性肝炎、肝炎后肝硬化、慢性重型肝炎、慢性肝炎中依次升高。血清GM-CSF浓度基本与WBC的计数量变化一致。结论:乙型肝炎患者血清GM-CSF水平对WBC水平的影响强于乙肝病毒,且有抗病毒作用。

 
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