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insecticidal protein
相关语句
  杀虫蛋白
     Identification of Bacillus thuringiensis vip3A Gene of the Vegetative Insecticidal Protein
     苏云金芽孢杆菌新型杀虫蛋白vip3A基因的鉴定
短句来源
     Studies on Purification, Gene Cloning and Expression of Insecticidal Protein
     粘质沙雷氏菌杀虫蛋白的分离纯化及其基因的克隆与表达研究
短句来源
     Degradation Dynamics of Cry1Ac Insecticidal Protein in Leaves of Bt Cotton under Different Environments
     转Bt-cry1Ac基因棉花叶片中杀虫蛋白在环境中的降解动态
短句来源
     Novel vegetative insecticidal protein Vip3A is a non-crystal and extracellular toxic protein secreted outside the cell during Bacillus thuringiensis mid-logarithmic growth.
     营养期杀虫蛋白(Vegetative Insecticidal Protein,Vip)Vip3A是苏云金芽孢杆菌(Bacillus thuringiensis,Bt)在营养生长对数中期分泌产生的一类新型杀虫蛋白
短句来源
     Residues of Cry1Ab Insecticidal Protein in Water Released from Bt Rice Tissue Litter and Surveying of Three Aquatic Insect Groups in Bt Rice Fields
     Bt稻Cry1Ab杀虫蛋白在水体中的残留和Bt稻田三类水生昆虫数量调查
短句来源
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  杀虫晶体蛋白
     Studies on the Enzyme-linked Immunosorbent Assay for Detection of Bacillus thuringiensis Insecticidal Protein Expressed in Transgenic Plants Ⅱ.Preparation of Polyclonal Antibody to Bt Insecticidal Protein
     转Bt基因植物中杀虫蛋白的酶联免疫检测技术研究Ⅱ.Bt杀虫晶体蛋白抗体的制备
短句来源
     In this paper, vegetative( vip83 ) and crystal(cry1Ac10 and cry1Ca) insecticidal protein genes from Bacillus thuri ngiensis were simultaneously electrospored into the plasmid-free strain BMB17 1. By the means of the specific P CR detection, the recombinant strains BMB2830-171 contained cry 1Ac10 and vip83, and BMB2 882-171 had cry1Ca and vip83 , were obtained respectively.
     将构建的营养期杀虫蛋白基因vip83表达质粒pBMB2 32 8和含杀虫晶体蛋白基因(cry1Ac1 0或cry1Ca)质粒同时电转化无质粒突变株BMB1 71并双抗筛选。 经PCR特异引物扩增验证 ,分别得到含cry1Ac1 0和vip83、cry1Ca和vip83的双基因重组菌BMB2 830 1 71和BMB2 882 1 71。
短句来源
     UV-Protection of Bt Melanin to Insecticidal Protein
     Bt黑色素对杀虫晶体蛋白的紫外保护
     A cryllel gene cloned from a Bacillus thuringiensis isolate in China and designated as by International Bt insecticidal Protein Gene Nomenclatural Committee was silent in host cell and encoded a protein consisting of 719 amino acids which was highly toxic to Plutella xylostella, Ostrinia furnacalis and Leguminivora glycinivorella larvae.
     cry1Ie1基因是我国鉴定分离并经国际命名的一种新型的苏云金芽孢杆菌(Bacillus thuringiensis,Bt)杀虫晶体蛋白基因。 该基因在Bt菌株中沉默,编码719个氨基酸组成的蛋白,对小菜蛾(Plutella xylostella)、玉米螟(Ostrinia furnacalis)和大豆食心虫(Leguminivora glycinivorella)等害虫具有较高的杀虫活性。
短句来源
     Acording to the amino acids sequence of Bacillus thuringiensis insecticidal protein Cry1A(b) and Cry1A(c), A fusion insecticidal gene GFM Cry1A with 1824bp has been synthesized. Plant expression vector pGB I 1214AB containing multi regulation elements has been constructed. In addition, another plant expression vector pGB I 1214ABC carrying bivalant insecticidal genes has been constructed, which harboring modified CpT I and GFM CrylA gene.
     根据苏云金芽胞杆菌杀虫晶体蛋白CrylA(b)和CrylA(c)的氨基酸序列,采用植物偏爱密码子,人工合成了全长1824bp的融合GFM CrylA Bt杀虫基因,并构建了带有多个表达调控元件的该基因高效植物表达载体pGBI1214AB,以及该基因和修饰豇豆胰蛋白酶抑制剂(CpTI)基因的双价杀虫基因植物表达载体pGBI1214ABC.
短句来源
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  杀虫蛋白基因
     The results show that single copy of Bt insecticidal protein gene has been integrated into the genome of T 0 transgenic plants and stably inherited.
     连续三代的研究结果表明 :Bt杀虫蛋白基因以单拷贝、杂合地整合到转基因植株 (T0 1,T0 2 ,T0 3,T0 5 ,T0 6 ,T0 7,T0 8)的基因组中 ,并稳定地遗传给后代 .
短句来源
     The progeny of transgenic rapeseed plants with Bt insecticidal protein gene was detected by kana mycin sifting and PCR. Results showed that single copy of Bt insecticidal protein gene has been integrated into the genome of T 0 transgenic plants and stably inherited.
     通过对转 Bt杀虫蛋白基因油菜植株的后代进行卡那霉素抗性分析和 PCR技术检测 ,结果表明 :Bt杀虫蛋白基因是以单拷贝、杂合地整合到转基因植株 (T0 1、T0 2、T0 3、T0 5、T0 6、T0 7、T0 8)的基因组中 ,并稳定地遗传。
短句来源
     In this paper, vegetative( vip83 ) and crystal(cry1Ac10 and cry1Ca) insecticidal protein genes from Bacillus thuri ngiensis were simultaneously electrospored into the plasmid-free strain BMB17 1. By the means of the specific P CR detection, the recombinant strains BMB2830-171 contained cry 1Ac10 and vip83, and BMB2 882-171 had cry1Ca and vip83 , were obtained respectively.
     将构建的营养期杀虫蛋白基因vip83表达质粒pBMB2 32 8和含杀虫晶体蛋白基因(cry1Ac1 0或cry1Ca)质粒同时电转化无质粒突变株BMB1 71并双抗筛选。 经PCR特异引物扩增验证 ,分别得到含cry1Ac1 0和vip83、cry1Ca和vip83的双基因重组菌BMB2 830 1 71和BMB2 882 1 71。
短句来源
     DYNAMICS OF EXPRESSION OF Bt INSECTICIDAL PROTEIN GENE IN THE TRANSGENIC RAPESEED AND ITS INSECT-RESISTANCE ACTIVITY
     Bt杀虫蛋白基因在转基因油菜中的动态表达与其抗虫性研究
短句来源
     Stable Heredity and Efficient Expression of Bt Insecticidal Protein Gene in the Transgenic Rapeseed and Its Insect-resistant Activity
     转基因抗虫油菜中Bt杀虫蛋白基因稳定遗传和高效表达及抗虫性研究
短句来源
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  “insecticidal protein”译为未确定词的双语例句
     The antifeeding was 68.39% and 74.75% while the insecticidal protein was 0.32mg/mL.
     当蛋白浓度为0.32mg/mL 时,24h 选择性拒食和非选择性拒食率分别为68.39%和74.75%。
短句来源
     1. GFMcry3Bb insecticidal protein gene was designed and synthesized.
     1.设计并人工合成Bt GFMcry3Bb杀虫基因。
短句来源
     Detection of the Vegetative Insecticidal Protein (Vip3A) Gene in Bacillus Thuringiensis Strains and Cloning of Vip3A Gene from Bt Strain WY-197
     苏云金芽孢杆菌Vip3A基因检测及BtWY-197菌株Vip3A基因克隆
短句来源
     receptors BMB171 and 4Q7 to obtain four engineered strains BMB8901-171, BMB8902-171, BMB8901-4Q7 and BMB8902-4Q7. SDS-PAGE results indicated that all recombinant strains express 88kD vegetative insecticidal protein.
     受体菌BMB171和 4Q7,获得了相应的工程菌BMB890 1 171,BMB890 2 171,BMB890 1 4Q7和BMB890 2 4Q7。 SDS PAGE电泳检测均有 88kD大小的蛋白表达。
短句来源
     Study on the Expression of Bt Insecticidal Protein of the Transgenic Insect-resistant Corn
     转基因抗虫玉米Bt蛋白表达量的研究
短句来源
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  insecticidal protein
The diversity of Bt strains facilitates isolation of new types of cry and vip (vegetative insecticidal protein) genes.
      
Transfer of an insecticidal protein gene ofBacillus thuringiensis into plant-colonizingAzospirillum
      
In accordance, the Cry9Aa2 insecticidal protein accumulated to high levels, ~10% of the total soluble cellular protein and ~20% in the membrane fraction.
      
Transgenic rice (Oryza sativa L.) plants generated through particle bombardment expressed high levels of an insecticidal protein (the snowdrop lectin, GNA) directed against sap-sucking insects.
      
Agrobacterium-mediated genetic transformation of Elymus breviaristatus with Pseudomonas pseudoalcaligenes insecticidal protein g
      
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P_2 insecticidal protein was isolated from the parasporal crystal of Bacillus thuringiemis var. kurstaki (HD-1) by DEAE-Sepharose column chromatography. It was first found out that the isolated P_2 insecticidal protein did not kill Spodoptera litura, but had low toxicity to Plutella xylostella, Manduca sexta and Bombyx mori, and high toxicity to Adoxophyes orana with LC_(50) up to 2.4ng/cm~2. The results also showed that the broad-spectrum insecticidal activity of crystal protein of HD-1...

P_2 insecticidal protein was isolated from the parasporal crystal of Bacillus thuringiemis var. kurstaki (HD-1) by DEAE-Sepharose column chromatography. It was first found out that the isolated P_2 insecticidal protein did not kill Spodoptera litura, but had low toxicity to Plutella xylostella, Manduca sexta and Bombyx mori, and high toxicity to Adoxophyes orana with LC_(50) up to 2.4ng/cm~2. The results also showed that the broad-spectrum insecticidal activity of crystal protein of HD-1 was mainly from P_1 insecticidal protein.

该研究采用DEAE-Sepharose柱层析的方法,从苏芸金杆菌HD-1菌株伴孢晶体中分离纯化得到P_2毒素蛋白。用5种鳞翅目昆虫分别测定了P_2毒素蛋白的杀虫活性,发现P_2毒素蛋白对斜纹夜蛾无效,对小菜蛾、烟草天蛾和家蚕的毒力也较低。但对苹果小卷叶蛾的毒力很高,半致死剂量仅为2.4ng/cm~2。实验结果还证明,HD-1菌株伴孢晶体的广谱杀虫性是P_1毒素蛋白起主要作用。

The Plasmids pGWFA11 and pGWFT18 containing modified insecticidal protein genes of Bacillus thuringiensis aizawal 7-29, were transformed into E. coli JM103. The insecticidal proteins determined by Western blotting, showed the expression of the modified strncturni genes in E. coil JM103. The bionssay of insecticidal proteins showed the 3′and 5′truncated insecticidal gene were better than the 3′truncated one in E. coli JM 103, and the expression of the genes promoted with tac prmootor was...

The Plasmids pGWFA11 and pGWFT18 containing modified insecticidal protein genes of Bacillus thuringiensis aizawal 7-29, were transformed into E. coli JM103. The insecticidal proteins determined by Western blotting, showed the expression of the modified strncturni genes in E. coil JM103. The bionssay of insecticidal proteins showed the 3′and 5′truncated insecticidal gene were better than the 3′truncated one in E. coli JM 103, and the expression of the genes promoted with tac prmootor was higher than those of the others. It is the first report that the modified insecticidal gene products gave follow-up deadly effects in the emergence of the fifth-instar larvae of Pieris rapae.

带有改造后的Rt.aizawai7-29杀虫基因的质粒pGWFT18、pGW FA11和pGHtA_2,转入E.coli JM103中,表达后,经生物定性杀虫试验,3′端和5′端全改造的杀虫基因比只改造3′端的杀虫基因具有更好的杀幼虫活性。而且在tac启动子启动下杀虫活性更强。本文首次报道改造后的杀虫基因产物对5龄菜青虫成蛹后羽化的成蝶具有较高的后致死效应作用。

Authors had sucessfully isolated and purified pseudomones pseudoalcaligenes insecticidal protein.The insecticidal protein is sensitive to heat and inactivited in 2 minutes at 80℃,it is stable at pH6.6~10.It has not haemdytic activity.

类产碱假单胞菌杀虫蛋白对蝗虫具有较强的杀伤力,在pH6.0~10及40℃以下,杀虫蛋白的活性是稳定的.该蛋白对胃蛋白酶敏感,对胰蛋白酶不敏感,不具溶血性

 
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