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   insecticidal protein 在 农业基础科学 分类中 的翻译结果: 查询用时:0.174秒
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insecticidal protein
相关语句
  杀虫蛋白
    Studies on the Enzyme-linked Immunosorbent Assay for Detection of Bacillus thuringiensis Insecticidal Protein Expressed in Transgenic Plants Ⅲ.Detection of Bt Insecticidal Protein by Indirect Enzyme-linked Immunosorbent Assay
    转Bt抗虫基因植物中杀虫蛋白的酶联免疫检测技术研究 Ⅲ.酶联免疫(ELISA)间接法检测Bt杀虫蛋白研究
短句来源
    Studies on the Enzyme-linked Immunosorbent Assay for Detection of Bacillus thuringiensis Insecticidal Protein Expressed in Transgenic Plants Ⅰ.Preparation of Bt Strain HD-1 Insecticidal Protein (Antigen)
    转Bt基因植物中杀虫蛋白的酶联免疫检测技术研究Ⅰ.Bt菌HD-1的杀虫晶体蛋白抗原制备
短句来源
    The antibody of Bt insecticidal protein prepared in this study is high specific.
    本研究所制备的Bt杀虫蛋白抗体具有高的特异性。
短句来源
    Bacillus thuringiensis Insecticidal Protein Levels in Different Tissue and Growing Period of Transgenic Cotton Determination Using ELISA
    转Bt棉不同生长期及不同器官杀虫蛋白表达量的免疫学方法测定
短句来源
    The goal of this research was to study on the preparation of Antigen of Bt insecticidal protein, production of antibody, and the test method technique for assay of the Bt toxin protein in transgenic cotton and other crop plants by using ELISA.
    本研究以检测目前抗虫转基因育种中研究最多、获得成效最大的转Bt抗虫基因的表达产物(杀虫蛋白)为检测目标。 为此研究了影响抗原和抗体制备的主要因素,制备出灵敏度高的特异抗体,在此基础上开展检测方法研究,掌握影响检测效果的有关因素,从而初步建立起灵敏度高的ELISA检测技术。
短句来源
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  杀虫晶体蛋白
    1. Bt insecticidal protein which was of high purity with a molecular weight of 130KD was prepared by using several biochemical techniques. 2. specific antibody was acquired by immunizing white rabbits with purified Bt insecticidal protein as antigen.
    首先用碱液(50mM Na_2CO_3,50mM EDTA,3%巯基乙醇)裂解孢晶混合物,调节PH至等电点沉淀出晶体杀虫蛋白,再用聚丙烯酰胺凝胶制备电泳进一步纯化粗提的杀虫晶体蛋白,经SDS-PAGE电泳分析显示只有一条带,说明获得的分子量约为130KD的杀虫晶体蛋白达到了电泳纯,为制备特异性抗体提供了条件保障。
短句来源
    The effects of cultural time, lysis solution and lytic conditions on production and purity of insecticidal protein from Bacillus thuringiensis strain HD-1 were investigated to get high quality insecticidal protein as a antigen.
    研究了培养时间对苏云金杆菌(Bacillusthuringiensis)HD-1菌株孢晶混合物的产量、杀虫晶体蛋白裂解液的种类和处理温度与时间对杀虫晶体蛋白提取率及纯度的影响。
短句来源
    The best lysis solution used to treat the complex of endospore and crystal protein is 50? mmol/L Na_2CO_3. There are lots of complex protein in insecticidal protein when 40? mmol/L NaOH lytic solution is used to lytic complex of endospore and crystal protein.
    采用50mmol/LNa2CO3裂解液处理孢晶混合物,可获得纯度高的130kD杀虫晶体蛋白,而用40mmol/LNaOH提取的杀虫晶体蛋白中含有较多杂蛋白。
短句来源
    The results show that when the pure Bt insecticidal protein is assayed by a indirect ELISA, there are no significant differences in sensitivity.
    结果表明,在采用纯的杀虫晶体蛋白进行灵敏度检测时,2种酶标抗体的测定结果非常相近,无明显差别,灵敏度可达7·8~15·6ng。 但在检测植物样本时,不同酶标抗体则存在差异。
短句来源
  “insecticidal protein”译为未确定词的双语例句
    Purification of antigen, preparation of specific antibody and enzyme-labelled antibody and ELISA method were studied for detection of Bt insecticidal protein expressed in Bt transgenic plants.
    本研究以目前抗虫转基因育种中研究最多的Bt CryIA(C)基因的表达产物为检测目标,在提取出高纯度抗原并制备出高特异性抗体的基础上,系统研究了两种载体上三种酶联免疫吸附测定方法的检测灵敏度,初步建立起快速、准确的酶联免疫检测技术。
短句来源
    1. GFMcry3Bb insecticidal protein gene was designed and synthesized.
    1.设计并人工合成Bt GFMcry3Bb杀虫基因。
短句来源
    Preliminary Study on Insecticidal Activity of Transgenic Corn Expressing Bt Insecticidal Protein Gene to Helicoverpa armigera Hübner
    转Bt基因抗虫玉米对棉铃虫杀虫活性的初步评价
短句来源
    Studies on the Enzyme-linked Immunosorbent Assay for Detection of Bacillus Thuringiensis Insecticidal Protein Expressed in Transgenic Plants
    转Bt抗虫基因植物的酶联免疫检测技术研究
短句来源
    Study on ELISA for Detection of Bacillus Thuringiensis Insecticidal Protein Expressed in Transgenic Plants
    转Bt抗虫基因植物的酶联免疫检测技术研究
短句来源
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  insecticidal protein
The diversity of Bt strains facilitates isolation of new types of cry and vip (vegetative insecticidal protein) genes.
      
Transfer of an insecticidal protein gene ofBacillus thuringiensis into plant-colonizingAzospirillum
      
In accordance, the Cry9Aa2 insecticidal protein accumulated to high levels, ~10% of the total soluble cellular protein and ~20% in the membrane fraction.
      
Transgenic rice (Oryza sativa L.) plants generated through particle bombardment expressed high levels of an insecticidal protein (the snowdrop lectin, GNA) directed against sap-sucking insects.
      
Agrobacterium-mediated genetic transformation of Elymus breviaristatus with Pseudomonas pseudoalcaligenes insecticidal protein g
      
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Three bioassay methods for monitoring the resistance of Helicoverpa armigera Hübner to Bacillus Thuringiensis Insecticidal Proteins were evaluated, ie dose response and growth inhibition assays with the test material incorporatied in diet and leaf dipping method. The results showed that the mortality from diet incorporation mothod using second instar larva is more suitable for resistance monitoring,and the best time for morality investigation was 5 days after Bt formulation treatment and 14 days after...

Three bioassay methods for monitoring the resistance of Helicoverpa armigera Hübner to Bacillus Thuringiensis Insecticidal Proteins were evaluated, ie dose response and growth inhibition assays with the test material incorporatied in diet and leaf dipping method. The results showed that the mortality from diet incorporation mothod using second instar larva is more suitable for resistance monitoring,and the best time for morality investigation was 5 days after Bt formulation treatment and 14 days after Cry1Ac treatment. The mortality of untreated larvae can be controlled below 20% by using improved artificial diet. Resistance Ratios were 4.6,3.9 and 3.8 folds to Cry1Ac and 1.1,1.4 and 1.6 folds to Bt formulation with Qiuxian,Jizhou and Gaomi populations in 1998 compared with a laboratory population using the diet incorporation mortality method.

对饲料混合法 ,体重抑制率法 ,浸叶接虫法进行比较研究 ,结果表明 ,饲料混合法比较稳定 ,比较适宜作为抗性测定的方法。用饲料混合法测定比较不同龄期幼虫的测定结果 ,确定 2龄作供试幼虫为宜。比较不同处理天数下的生测结果 ,确定 Bt粉剂对 2龄幼虫的适宜处理时间为 5d,Cry1Ac为 14d。用改进稀饲料隔离饲养供试幼虫的方法后 ,得到理想的生测数据 ,生测时间延长到 14d,对照死亡率也能控制在 2 0 %以下。从我们 1998年对棉铃虫的抗性监测结果说明 ,尽管田间棉铃虫对 Bt ICP未达抗性水平 ,但对 Cry1Ac型蛋白敏感性已有降低 ,值得引起重视

The genetic engineering of plant proteinase inhibitor (PI) provides a novel approach, in addition to that of microbial toxin protein genes, for improving plant resistance to insect pests. In camparison with other insecticidal proteins wildly used in the world, especially Bt endotoxins, the plant PI possesses some distinctive advantages such as hard to produce tolerance, broad target spectrum , inductive enhancement, and not so much gene modification, etc. While the resistance mechanism of Pis to...

The genetic engineering of plant proteinase inhibitor (PI) provides a novel approach, in addition to that of microbial toxin protein genes, for improving plant resistance to insect pests. In camparison with other insecticidal proteins wildly used in the world, especially Bt endotoxins, the plant PI possesses some distinctive advantages such as hard to produce tolerance, broad target spectrum , inductive enhancement, and not so much gene modification, etc. While the resistance mechanism of Pis to insect pests is not so clear so far, and there are a number of factors affecting on their insecticidal actions. In this paper, the recent progress of the studies on insect resistance functions and mechanisms was cited and analyzed, and the problems and solutions in plant PI genetic engineering were discussed.

蛋白酶抑制剂抗虫基因工程为提高植物的抗虫能力提供了一种新的策略。与其它来源的抗虫蛋白相比,植物蛋白酶抑制剂有很多独特的优点。蛋白酶抑制剂的抗虫机理尚不完全清楚,影响其抗虫效果的因素也很多。文中列举并分析了PI抗虫作用的机理以及目前研究的新进展,提出植物蛋白酶抑制剂抗虫基因工程中存在的问题和进一步解决的办法。

Many important crops have been genetically engineered to produce various proteins, such as Bacillus thuringiensis endotoxin, to increase their resistance to pests. The fate and persistence of insecticidal protein in transgenetic plants in the soil is an important issue because of possible direct and indirect effects of these insecticidal proteins on soil organisms. In addition, there is the potential for prolonged exposure of soil organisms to the insecticidal protein because of possible plant...

Many important crops have been genetically engineered to produce various proteins, such as Bacillus thuringiensis endotoxin, to increase their resistance to pests. The fate and persistence of insecticidal protein in transgenetic plants in the soil is an important issue because of possible direct and indirect effects of these insecticidal proteins on soil organisms. In addition, there is the potential for prolonged exposure of soil organisms to the insecticidal protein because of possible plant residue remaining in the soil post-harvest and post tillage. Furthermore, genetic engineering and tissue culturing of plants occasionally have caused unexpected changes in plant characteristics, including nutrition quality. Such changes could affect plant decomposition rate and carbon and nitrogen levels which in turn could impact soil fertility, processes and organisms. Rice is an agronomically important crop in China. In practice, rice straws are usually embedded into the soil to enhance the soil fertility. As a result, the toxins as well as the other expressed products may accumulate in the soil and could pose a hazard to the non-target soil microorganisms. To investigated the potential ecological impact of straw decomposition of transgenic plant on microbial flora, we conducted an experiment of influences of the straw decomposition of Bt transgenic rice (Bt-rice) and its parent (non-Bt-rice) on microbial flora under the laboratory conditions during 2001~2002. Transgenic and parental rice straw and soil were collected from the field. Straw and soil were air-dried in room temperature and ground into powder. The powder of straw and soil were mixed at the rate of 4∶100. The mixture was dispensed into a series of 250ml flasks with 250g per flask. The moisture of the medium was adjusted to 100 and was cultivated in 25℃. The Bt content, the population of bacteria, actinomyces, fungi and the activity of denitrifying bacteria and phosphate solubilizing microorganism were determined. The results showed as follows: (1) The concentration of Bt protein in the mixture of straw and soil decreased rapidly within 0~17 days (15.86ng/g for the third day and 6.864 ng/g for the twelfth day),then the decomposition became slower and mainteined at the level of 6.728~6.196ng/g. The changing trend of Bt was similar to the result of Palm's(1996). (2) There was a similar trend of bacterial number (CFU) during decomposition between Bt-rice, non-Bt rice and control, viz.arise at first stage and descend subsequently. Significant difference was found in the CFU of bacteria between Bt-rice and non-Bt rice, the CFU of non-Bt rice was higher than that of Bt rice (P<0.05). (3) Except the third day and the sixth day of the initial decomposition, the CFU of fungi in Bt-rice was significantly higher than that of non-Bt rice and control, the result was similar to that Katherine reported (1996). (4) No significant regular was found in the CFU of actinomyces during the decomposition, there was a undulation in the CFU in non-Bt rice. Except the sixth day, ninth day,twenty-sixth day and thirty-fifth day, the CFU of non-Bt rice was significantly higher than that of Bt-rice (P<0.05). (5) The activity of denitrifying bacteria in non-Bt-rice was significantly higher than that of Bt-rice (P<0.05) at some sampling stage, no significant difference was found in the CFU and activity of phosphate solubilizing microorganisms between Bt-rice and non-Bt rice.Studies showed that the transgenic plants could cause the changes of microbial flora in soil ecosystem by remaining the straw and leaves in soil. So the influence of transgenic plants on soil micro-ecosystem should be clarified before commerical application. The nutrients released from straw decomposition can provide nature substrate for micro-organism in soil. In this study, no matter Bt-rice or non-Bt rice, the CFU of microbe in soil which contained straw was significantly higher than that of control without any straw. Significant difference was found in CFU of bacteria and fungi between Bt-rice and non-Bt rice, the CFU of

以高抗螟虫、已释放应用的 Bt水稻 (克螟稻 1号 )及其亲本 (非 Bt水稻 )为材料 ,在实验室条件下研究了转 Bt基因水稻及其亲本秸秆在降解过程中对土壤微生物主要类群的影响 ,这些类群包括细菌、真菌、放线菌、反硝化细菌、解磷微生物。结果表明 :(1)降解过程中 Bt蛋白浓度在前两周内迅速下降 ,随后降解速度变慢 ,17d以后至 5 3d,Bt蛋白浓度基本上保持在 6 .72 8~6 .196 ng/ g的水平。 (2 )秸秆降解过程中 ,不同处理细菌数量的变化趋势相似 ,转基因水稻与其亲本之间差异显著 ,非转基因细菌数量高于转基因细菌数量。(3)除降解初期第 3天、第 6天之外 ,其他取样时期的转基因水稻真菌数量要显著高于非转基因和对照。(4)放线菌数量没有明显变化规律 ,除第 6、9、2 6、35天外 ,非转基因数量显著高于转基因。(5 )非转基因秸秆降解反硝化细菌活性高于转基因 ,而解磷微生物活性处理之间无明显差异。

 
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