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insecticidal protein
相关语句
  杀虫蛋白
    Studies on Purification, Gene Cloning and Expression of Insecticidal Protein
    粘质沙雷氏菌杀虫蛋白的分离纯化及其基因的克隆与表达研究
短句来源
    The Spatio-Temporal Distribution of Bt (Bacillus thuringiensis) Insecticidal Protein and the Effect of Transgenic Bt Cotton on Bollworm Resistance
    转Bt基因棉杀虫蛋白含量时空分布及对棉铃虫产生抗性的影响
短句来源
    Identification of Bacillus thuringiensis vip3A Gene of the Vegetative Insecticidal Protein
    苏云金芽孢杆菌新型杀虫蛋白vip3A基因的鉴定
短句来源
    Characteristic Analysis of Vegetative Insecticidal Protein Gene from Bacillus thuringiensis
    苏云金芽胞杆菌营养期杀虫蛋白基因特性分析
短句来源
    Residues of Cry1Ab Insecticidal Protein in Water Released from Bt Rice Tissue Litter and Surveying of Three Aquatic Insect Groups in Bt Rice Fields
    Bt稻Cry1Ab杀虫蛋白在水体中的残留和Bt稻田三类水生昆虫数量调查
短句来源
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  杀虫晶体蛋白
    UV-Protection of Bt Melanin to Insecticidal Protein
    Bt黑色素对杀虫晶体蛋白的紫外保护
    A cryllel gene cloned from a Bacillus thuringiensis isolate in China and designated as by International Bt insecticidal Protein Gene Nomenclatural Committee was silent in host cell and encoded a protein consisting of 719 amino acids which was highly toxic to Plutella xylostella, Ostrinia furnacalis and Leguminivora glycinivorella larvae.
    cry1Ie1基因是我国鉴定分离并经国际命名的一种新型的苏云金芽孢杆菌(Bacillus thuringiensis,Bt)杀虫晶体蛋白基因。 该基因在Bt菌株中沉默,编码719个氨基酸组成的蛋白,对小菜蛾(Plutella xylostella)、玉米螟(Ostrinia furnacalis)和大豆食心虫(Leguminivora glycinivorella)等害虫具有较高的杀虫活性。
短句来源
    The crySCa gene from HBF-1 strain was expressed in Bt HD-73". The expressed product showed toxic to Ahomala corpulenta larvae with 68.3% corrected mortality in 160pig/g soil concentration. A novel cryS gene was cloned from Bt strain 185. The homology between the sequence of it and known cry8 genes was less than 78%, so it was designed as crySEal gene (AY329081) by International Bt insecticidal Protein Gene Nomenclatural Committee.
    该基因与已知cry8基因的同源性均低于78%,已在GenBank登录(AY329081),并被国际Bt杀虫晶体蛋白基因命名委员会命名为cry8Ea1基因。 这是我国发现的第一个第二等级的Bt杀虫基因,杀虫活性的研究正在进行。
短句来源
  杀虫蛋白基因
    Characteristic Analysis of Vegetative Insecticidal Protein Gene from Bacillus thuringiensis
    苏云金芽胞杆菌营养期杀虫蛋白基因特性分析
短句来源
    Using Southern blot of gene localization, vegetative insecticidal protein gene was determined neither on chromosome nor circle plasmids.
    通过Southern杂交的基因定位实验 ,证实了营养期杀虫蛋白基因并非位于染色体和环型质粒上 ;
短句来源
    In the conclusion, these results indidate that vegetative insecticidal protein gene is highly conserved and is distributed broader in the natural strains of Bacillus thuringiensis, and the specific structure of its coded sequence implied potential function,which contributed to further making vegetative insecticidal protein clear.
    上述结果提示 ,营养期杀虫蛋白基因在苏云金芽胞杆菌天然菌株中高度保守并较为广泛分布 ,其编码蛋白的特殊结构提示了营养期杀虫蛋白潜在的功能特性 ,为进一步研究营养期杀虫蛋白奠定了基础 .
短句来源
  “insecticidal protein”译为未确定词的双语例句
    P_2 INSECTICIDAL PROTEIN FROM PARASPORAL CRYSTAL OF BACILLUS THURINGIENSIS
    苏芸金杆菌伴孢晶体中P_2毒素蛋白的研究
短句来源
    Studies on Spatial-temporal Dynamics of Insecticidal Protein Expression of Bt Corn and Its Degradation in Soil
    Bt玉米抗虫蛋白表达的时空动态及其土壤降解研究
短句来源
    Detection of the Vegetative Insecticidal Protein (Vip3A) Gene in Bacillus Thuringiensis Strains and Cloning of Vip3A Gene from Bt Strain WY-197
    苏云金芽孢杆菌Vip3A基因检测及BtWY-197菌株Vip3A基因克隆
短句来源
    The contents ofBt insecticidal protein and gossypol in the Fi generation of GK12crossed with high-gossypol cotton line HG-BR-8 and with low-gossypolcotton line 471219 were utilized to analyse the inner relationship withtheir bollworm resistance.
    对GK12分别和高酚棉HG-BR-8、低酚棉471219的杂交F_1代进行Bt毒蛋白和棉酚含量测定,分析它们对转Bt基因棉的抗性影响;
短句来源
    1. GFMcryllB insecticidal protein gene was designed and synthesized.
    1.设计并人工合成Bt GFMcryllB杀虫基因。
短句来源
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  insecticidal protein
The diversity of Bt strains facilitates isolation of new types of cry and vip (vegetative insecticidal protein) genes.
      
Transfer of an insecticidal protein gene ofBacillus thuringiensis into plant-colonizingAzospirillum
      
In accordance, the Cry9Aa2 insecticidal protein accumulated to high levels, ~10% of the total soluble cellular protein and ~20% in the membrane fraction.
      
Transgenic rice (Oryza sativa L.) plants generated through particle bombardment expressed high levels of an insecticidal protein (the snowdrop lectin, GNA) directed against sap-sucking insects.
      
Agrobacterium-mediated genetic transformation of Elymus breviaristatus with Pseudomonas pseudoalcaligenes insecticidal protein g
      
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P_2 insecticidal protein was isolated from the parasporal crystal of Bacillus thuringiemis var. kurstaki (HD-1) by DEAE-Sepharose column chromatography. It was first found out that the isolated P_2 insecticidal protein did not kill Spodoptera litura, but had low toxicity to Plutella xylostella, Manduca sexta and Bombyx mori, and high toxicity to Adoxophyes orana with LC_(50) up to 2.4ng/cm~2. The results also showed that the broad-spectrum insecticidal activity of crystal protein of HD-1...

P_2 insecticidal protein was isolated from the parasporal crystal of Bacillus thuringiemis var. kurstaki (HD-1) by DEAE-Sepharose column chromatography. It was first found out that the isolated P_2 insecticidal protein did not kill Spodoptera litura, but had low toxicity to Plutella xylostella, Manduca sexta and Bombyx mori, and high toxicity to Adoxophyes orana with LC_(50) up to 2.4ng/cm~2. The results also showed that the broad-spectrum insecticidal activity of crystal protein of HD-1 was mainly from P_1 insecticidal protein.

该研究采用DEAE-Sepharose柱层析的方法,从苏芸金杆菌HD-1菌株伴孢晶体中分离纯化得到P_2毒素蛋白。用5种鳞翅目昆虫分别测定了P_2毒素蛋白的杀虫活性,发现P_2毒素蛋白对斜纹夜蛾无效,对小菜蛾、烟草天蛾和家蚕的毒力也较低。但对苹果小卷叶蛾的毒力很高,半致死剂量仅为2.4ng/cm~2。实验结果还证明,HD-1菌株伴孢晶体的广谱杀虫性是P_1毒素蛋白起主要作用。

The Plasmids pGWFA11 and pGWFT18 containing modified insecticidal protein genes of Bacillus thuringiensis aizawal 7-29, were transformed into E. coli JM103. The insecticidal proteins determined by Western blotting, showed the expression of the modified strncturni genes in E. coil JM103. The bionssay of insecticidal proteins showed the 3′and 5′truncated insecticidal gene were better than the 3′truncated one in E. coli JM 103, and the expression of the genes promoted with tac prmootor was...

The Plasmids pGWFA11 and pGWFT18 containing modified insecticidal protein genes of Bacillus thuringiensis aizawal 7-29, were transformed into E. coli JM103. The insecticidal proteins determined by Western blotting, showed the expression of the modified strncturni genes in E. coil JM103. The bionssay of insecticidal proteins showed the 3′and 5′truncated insecticidal gene were better than the 3′truncated one in E. coli JM 103, and the expression of the genes promoted with tac prmootor was higher than those of the others. It is the first report that the modified insecticidal gene products gave follow-up deadly effects in the emergence of the fifth-instar larvae of Pieris rapae.

带有改造后的Rt.aizawai7-29杀虫基因的质粒pGWFT18、pGW FA11和pGHtA_2,转入E.coli JM103中,表达后,经生物定性杀虫试验,3′端和5′端全改造的杀虫基因比只改造3′端的杀虫基因具有更好的杀幼虫活性。而且在tac启动子启动下杀虫活性更强。本文首次报道改造后的杀虫基因产物对5龄菜青虫成蛹后羽化的成蝶具有较高的后致死效应作用。

Three bioassay methods for monitoring the resistance of Helicoverpa armigera Hübner to Bacillus Thuringiensis Insecticidal Proteins were evaluated, ie dose response and growth inhibition assays with the test material incorporatied in diet and leaf dipping method. The results showed that the mortality from diet incorporation mothod using second instar larva is more suitable for resistance monitoring,and the best time for morality investigation was 5 days after Bt formulation treatment and 14 days after...

Three bioassay methods for monitoring the resistance of Helicoverpa armigera Hübner to Bacillus Thuringiensis Insecticidal Proteins were evaluated, ie dose response and growth inhibition assays with the test material incorporatied in diet and leaf dipping method. The results showed that the mortality from diet incorporation mothod using second instar larva is more suitable for resistance monitoring,and the best time for morality investigation was 5 days after Bt formulation treatment and 14 days after Cry1Ac treatment. The mortality of untreated larvae can be controlled below 20% by using improved artificial diet. Resistance Ratios were 4.6,3.9 and 3.8 folds to Cry1Ac and 1.1,1.4 and 1.6 folds to Bt formulation with Qiuxian,Jizhou and Gaomi populations in 1998 compared with a laboratory population using the diet incorporation mortality method.

对饲料混合法 ,体重抑制率法 ,浸叶接虫法进行比较研究 ,结果表明 ,饲料混合法比较稳定 ,比较适宜作为抗性测定的方法。用饲料混合法测定比较不同龄期幼虫的测定结果 ,确定 2龄作供试幼虫为宜。比较不同处理天数下的生测结果 ,确定 Bt粉剂对 2龄幼虫的适宜处理时间为 5d,Cry1Ac为 14d。用改进稀饲料隔离饲养供试幼虫的方法后 ,得到理想的生测数据 ,生测时间延长到 14d,对照死亡率也能控制在 2 0 %以下。从我们 1998年对棉铃虫的抗性监测结果说明 ,尽管田间棉铃虫对 Bt ICP未达抗性水平 ,但对 Cry1Ac型蛋白敏感性已有降低 ,值得引起重视

 
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