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insecticidal protein
相关语句
  杀虫蛋白
    The Acquirement of Transgenic Bollworm Resistant Xinjiang Cotton Plants with the Synthetic Gene Coding Bacillus Thuringiensis Insecticidal Protein
    Bt杀虫蛋白基因导入新疆棉花获得抗虫转基因植株
短句来源
    The Study of Bacillus Thuringiensis Insecticidal Protein Expressed in the Tissue of Xinjiang Transgenic Insect Resistant Cotton
    新疆转基因抗虫棉组织中Bt杀虫蛋白表达的研究
短句来源
    DYNAMICS OF EXPRESSION OF Bt INSECTICIDAL PROTEIN GENE IN THE TRANSGENIC RAPESEED AND ITS INSECT-RESISTANCE ACTIVITY
    Bt杀虫蛋白基因在转基因油菜中的动态表达与其抗虫性研究
短句来源
    Stable Heredity and Efficient Expression of Bt Insecticidal Protein Gene in the Transgenic Rapeseed and Its Insect-resistant Activity
    转基因抗虫油菜中Bt杀虫蛋白基因稳定遗传和高效表达及抗虫性研究
短句来源
    Degradation Dynamics of Cry1Ac Insecticidal Protein in Leaves of Bt Cotton under Different Environments
    转Bt-cry1Ac基因棉花叶片中杀虫蛋白在环境中的降解动态
短句来源
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  杀虫晶体蛋白
    Acording to the amino acids sequence of Bacillus thuringiensis insecticidal protein Cry1A(b) and Cry1A(c), A fusion insecticidal gene GFM Cry1A with 1824bp has been synthesized. Plant expression vector pGB I 1214AB containing multi regulation elements has been constructed. In addition, another plant expression vector pGB I 1214ABC carrying bivalant insecticidal genes has been constructed, which harboring modified CpT I and GFM CrylA gene.
    根据苏云金芽胞杆菌杀虫晶体蛋白CrylA(b)和CrylA(c)的氨基酸序列,采用植物偏爱密码子,人工合成了全长1824bp的融合GFM CrylA Bt杀虫基因,并构建了带有多个表达调控元件的该基因高效植物表达载体pGBI1214AB,以及该基因和修饰豇豆胰蛋白酶抑制剂(CpTI)基因的双价杀虫基因植物表达载体pGBI1214ABC.
短句来源
  杀虫蛋白基因
    The Acquirement of Transgenic Bollworm Resistant Xinjiang Cotton Plants with the Synthetic Gene Coding Bacillus Thuringiensis Insecticidal Protein
    Bt杀虫蛋白基因导入新疆棉花获得抗虫转基因植株
短句来源
    DYNAMICS OF EXPRESSION OF Bt INSECTICIDAL PROTEIN GENE IN THE TRANSGENIC RAPESEED AND ITS INSECT-RESISTANCE ACTIVITY
    Bt杀虫蛋白基因在转基因油菜中的动态表达与其抗虫性研究
短句来源
    Stable Heredity and Efficient Expression of Bt Insecticidal Protein Gene in the Transgenic Rapeseed and Its Insect-resistant Activity
    转基因抗虫油菜中Bt杀虫蛋白基因稳定遗传和高效表达及抗虫性研究
短句来源
    The results show that single copy of Bt insecticidal protein gene has been integrated into the genome of T 0 transgenic plants and stably inherited.
    连续三代的研究结果表明 :Bt杀虫蛋白基因以单拷贝、杂合地整合到转基因植株 (T0 1,T0 2 ,T0 3,T0 5 ,T0 6 ,T0 7,T0 8)的基因组中 ,并稳定地遗传给后代 .
短句来源
    By analysing F 2 of hybrid combination of homozygous transgenic plants, Bt insecticidal protein gene has been integrated into different locus of homologous chromosome of T 01 and T 05 or T 03 and T 08 transgenic rapeseed plants,but has been integrated into heterologous chromosome.
    纯合转基因株系杂交分析表明 ,在不同 T0 转基因植株中 Bt杀虫蛋白基因整合位点是不同的 ,T0 1和 T0 5或 T0 3和 T0 8的 Bt杀虫蛋白基因整合位点是同源染色体的不同座位 ,而其他转基因植株的 Bt杀虫蛋白基因整合位点是在非同源染色体上
短句来源
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  “insecticidal protein”译为未确定词的双语例句
    Establishment of Agrobacterium-Mediated Genetic Transformation Systems in Forage Plants with Insecticidal Protein Gene
    几种禾本科牧草遗传转化体系的建立和抗虫转基因研究
短句来源
    Genetic Mode of Bt Insecticidal Protein Gene in the Insect-Resistant Transgenic Rapeseed
    外源基因在转基因抗虫油菜中的遗传行为
短句来源
    Content and Expression of Bacillus thuringiensis Insecticidal Protein in Transgenic Cotton
    转Bt抗虫棉各器官毒蛋白的含量及表达
短句来源
    Expression of Insecticidal Protein of Bacillus Thuringiensis in Bt Transgenic Soybean
    转Bt基因大豆植株中Bt毒蛋白的表达
短句来源
    Study on the Expression of Bt Insecticidal Protein of the Transgenic Insect-resistant Corn
    转基因抗虫玉米Bt蛋白表达量的研究
短句来源
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  insecticidal protein
The diversity of Bt strains facilitates isolation of new types of cry and vip (vegetative insecticidal protein) genes.
      
Transfer of an insecticidal protein gene ofBacillus thuringiensis into plant-colonizingAzospirillum
      
In accordance, the Cry9Aa2 insecticidal protein accumulated to high levels, ~10% of the total soluble cellular protein and ~20% in the membrane fraction.
      
Transgenic rice (Oryza sativa L.) plants generated through particle bombardment expressed high levels of an insecticidal protein (the snowdrop lectin, GNA) directed against sap-sucking insects.
      
Agrobacterium-mediated genetic transformation of Elymus breviaristatus with Pseudomonas pseudoalcaligenes insecticidal protein g
      
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Based on the reaction among antibody, antigen and enzyme antibody, an indirect enzyme linked immunosorbent assay (ELISA) method was developed to detect and quantitate the Bacillus thuringiensis (Bt) insecticidal protein expressed in the tissue of Bt transgenic insect resistant cotton. Young leaves from transgenic insect resistant cotton of strain 310 at initial squaring stage were analyzed by ELISA, and showed that the content of...

Based on the reaction among antibody, antigen and enzyme antibody, an indirect enzyme linked immunosorbent assay (ELISA) method was developed to detect and quantitate the Bacillus thuringiensis (Bt) insecticidal protein expressed in the tissue of Bt transgenic insect resistant cotton. Young leaves from transgenic insect resistant cotton of strain 310 at initial squaring stage were analyzed by ELISA, and showed that the content of Bt insecticidal protein in the leaves averaged up to 0 126% of the total soluble protein. This explains the insect resistance of the cotton strain 310 which has been developed by introduction of Bt insecticidal protein gene through the pollen tube pathway into a common cotton cultivar. The minimal detectable value of purified Bt insecticidal protein was 20 ng/ml by this method.

利用抗体-抗原-酶标抗体反应,建立了间接酶联免疫吸附测定法(ELISA),以检测转基因抗虫棉品系310幼叶Bt毒蛋白含量。初步测定结果:转基因抗虫棉幼叶中Bt毒蛋白含量为总可溶性蛋白质的(0.126±0.008)%。提纯的伴胞晶体样品经ELISA法检测,其Bt毒蛋白最低可检值为20ng/ml。这一检测技术既可对转基因抗虫棉组织中Bt毒蛋白表达量进行定性、定量测定,也为阐明转基因抗虫棉的抗虫特性提供了理论依据。

By the pollon tube pathway, the artificially synthesized Bacillus thuringiensis insecticidal protein gene was transformed into the elite Xinjiang cotton( Gossypium hirsutum L.) cultivars namely Xinluzao No.4 and C6524, and 20 thousand injected cotton seeds were obtained. After the biological inspection by raised bollworm ( Helicoverpa armigera ), 18 Xinluzao No.4 plants and 36 C6524 plants resisting to bollworms were got. With a pair of primers of Bt gene, the results of PCR analysis indicated...

By the pollon tube pathway, the artificially synthesized Bacillus thuringiensis insecticidal protein gene was transformed into the elite Xinjiang cotton( Gossypium hirsutum L.) cultivars namely Xinluzao No.4 and C6524, and 20 thousand injected cotton seeds were obtained. After the biological inspection by raised bollworm ( Helicoverpa armigera ), 18 Xinluzao No.4 plants and 36 C6524 plants resisting to bollworms were got. With a pair of primers of Bt gene, the results of PCR analysis indicated that the Bt gene did integrate within the genenome of the total 54 transformed bollworm resistant cotton plants. We primarily got the transgenic bollworm resistant Xinjiang cotton plant.

通过花粉管通道法将人工全序合成的Bt杀虫蛋白基因导入新陆早4号和C6524两品种中,收获注射过Bt杀虫蛋白基因的棉花种子2万余粒。经棉铃虫饲喂的抗虫性生物检测,得到高抗虫的18株新陆早4号转化苗和36株C6524转化苗;以Bt基因的一对引物进行PCR分析,54株抗棉铃虫的转化棉苗均扩增出部分Bt杀虫蛋白基因的目标带。未转化的对照棉苗则无此条带,证实Bt杀虫蛋白基因已整合到转化抗虫棉株的染色体上,首次获得了新疆转目的基因的高效抗虫棉。

An antibody sandwich enzyme linked immunosorbent assay (ELISA) was developed to detect and quantitate the Bacillus thuringiensis (Bt) insecticidal protein expressed in transgenic insect resistant cotton using a purified Bt toxin (67kDa) as standard protein and antigen. The minimal detectable concentration of purified Bt toxin was 1μg·L -1 and the linear assay range of the method was 1μg·L -1 to 15μg·L -1 .Both the errors of intra and interplate(s) were <5%,and the mean recovery...

An antibody sandwich enzyme linked immunosorbent assay (ELISA) was developed to detect and quantitate the Bacillus thuringiensis (Bt) insecticidal protein expressed in transgenic insect resistant cotton using a purified Bt toxin (67kDa) as standard protein and antigen. The minimal detectable concentration of purified Bt toxin was 1μg·L -1 and the linear assay range of the method was 1μg·L -1 to 15μg·L -1 .Both the errors of intra and interplate(s) were <5%,and the mean recovery rate was 94.94%.It was indicated by quality controal experiments that this ELISA method was stable and reliable ,and it can be used in the detectiomn of Bt toxin content expressed in transgenic cotto.

利用纯化的Bt毒素蛋白(67kDa)作为标准蛋白和免疫抗原,建立了检测转基因抗虫棉植株内Bt毒蛋白含量的抗体夹心ELISA方法。检测极限1μg·L- 1,线性范围1- 15μg·L- 1,板内误差C.V.< 5% ,板间误差C.V.< 5% ,平均样品回收率94.94% 。经稀释度试验等质量控制试验,表明所建立的ELISA方法是可靠的,可用于转基因棉花植株中Bt毒素蛋白含量的测定。

 
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