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insecticidal protein
相关语句
  杀虫蛋白
    The leaves from 12 transgenic insect-resistant poplars were analyzed by ELISA and showed that the expressing quantity of Bt insecticidal protein exist distinction . The Bt insecticidal protein of D18, FB51 and FB56 exceed 6700 ng /g fresh leaf, which is 0.090%, 0.036% and 0.034% of plant soluble protein respectively.
    ELISA分析表明,所检测的转基因106杨树无性系中Bt毒蛋白表达存在着差异,其中转基因植株D18、FB51、FB56叶片中杀虫蛋白含量超过6700ng/g鲜叶,约分别占植物叶片可溶性总蛋白的0.090%、0.036%、0.034%。
短句来源
    The leaves from 12 transgenic insect-resistant poplars were analyzed by ELISA and showed that the expressing quantity of Bt insecticidal protein in D18 is 6 754 ng /g fresh leaf (0.090% of plant soluble protein), which is the highest in the transgenic clones with two-insect-resistant genes.
    在检测的 12个转基因杨树无性系中 ,ELISA结果表明 ,转双价基因杨树无性系中以D18的Bt杀虫蛋白表达量最高 ,Bt杀虫蛋白含量达 6 75 4ng/g鲜叶 ,约占植物可溶性蛋白的 0 0 90 % ;
短句来源
    The insecticidal mechanism of transgenic poplars was discussed by determining the influence of transgenic poplars on detoxification enzyme and protective enzyme in the midgut and haemolymph of larvae. Moreover, the expression of insecticidal protein in the transgenic poplars was also examined in this paper. Main results are listed as following:
    通过检测转基因杨树对幼虫中肠和血淋巴解毒酶、保护酶活性影响以及杀虫蛋白在转基因杨树大田移植植株中的表达情况,研究转基因杨树对杨小舟蛾的杀虫机理。
短句来源
  “insecticidal protein”译为未确定词的双语例句
    This is the first report on insect resistant transgenic hybrid poplar 741 that expresses two insecticidal protein genes.
    这是国内外首次报道用双抗虫基因获得的抗虫 741毛白杨植株。
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  insecticidal protein
The diversity of Bt strains facilitates isolation of new types of cry and vip (vegetative insecticidal protein) genes.
      
Transfer of an insecticidal protein gene ofBacillus thuringiensis into plant-colonizingAzospirillum
      
In accordance, the Cry9Aa2 insecticidal protein accumulated to high levels, ~10% of the total soluble cellular protein and ~20% in the membrane fraction.
      
Transgenic rice (Oryza sativa L.) plants generated through particle bombardment expressed high levels of an insecticidal protein (the snowdrop lectin, GNA) directed against sap-sucking insects.
      
Agrobacterium-mediated genetic transformation of Elymus breviaristatus with Pseudomonas pseudoalcaligenes insecticidal protein g
      
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Partially modified Bt Cry1Ac gene and the arrowhead proteinase inhibitor (API) gene were used to construct a plant transformation vector pBtiA and this construct was transferred into the genome of the hybrid poplar 741 [ Populus alba L.×( P. davidiana Dode+ P. simonii Carr.)× P. tomentosa Carr.] by Agrobacterium _ mediated transformation. Ten kanamycin resistant plants have been regenerated. Upon insect bioassay using Clostera anachoreta (Fabricius), three of the examined plants were...

Partially modified Bt Cry1Ac gene and the arrowhead proteinase inhibitor (API) gene were used to construct a plant transformation vector pBtiA and this construct was transferred into the genome of the hybrid poplar 741 [ Populus alba L.×( P. davidiana Dode+ P. simonii Carr.)× P. tomentosa Carr.] by Agrobacterium _ mediated transformation. Ten kanamycin resistant plants have been regenerated. Upon insect bioassay using Clostera anachoreta (Fabricius), three of the examined plants were demonstrated to be highly resistant to the testing insects. The mortality of insect larvae on one plant was higher than 90% in 6 days after infestation and the growth of the survival larvae were seriously inhibited. Results of PCR and Southern blot analysis indicated that both Bt Cry1Ac gene and API gene were integrated as a single copy into the genomes of these three plants when Cry1Ac gene fragment was used as the probe. Protein dot blot immunoassay and ELISA analysis revealed that at least the Cry1Ac protein was produced in these three transgenic plants and the expression levels were estimated to be approximately 0.015% of the leaf total soluble protein. This is the first report on insect resistant transgenic hybrid poplar 741 that expresses two insecticidal protein genes.

用部分改造后的苏云金芽孢杆菌 (Bt)杀虫蛋白基因和慈菇蛋白酶抑制剂 (API)基因A构建了植物表达载体。然后通过根癌土壤杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn .)介导将此表达载体上的双抗虫基因转入杂种 741毛白杨 [PopulusalbaL .× (P .davidianaDode +P .simoniiCarr.)×P .tomentosaCarr.]获得了一批抗卡那霉素的转化再生植株。用杨扇舟蛾 (Closteraanachoreta (Fabricius) )进行虫试的结果表明有 3株抗虫杨树 ,其中有1株杨树的叶片可使试虫在 6天内的死亡率达 90 %以上 ,而且存活幼虫的生长发育受到了明显的抑制。PCR检测及基因组DNASouthern杂交分析的结果都表明Bt杀虫蛋白基因和API基因已整合到以上 3株抗虫杨树的基因组中 ,而且表现为单拷贝整合。用Bt毒蛋白抗血清进行滤膜免疫反应及ELISA检测结果表明 3株转基因杨树都有Bt杀虫蛋白的表达 ,表达量约占叶总可溶性蛋白的 0 .0 15 %。这是国内外首次报道用双抗虫基因获得的抗虫 7...

用部分改造后的苏云金芽孢杆菌 (Bt)杀虫蛋白基因和慈菇蛋白酶抑制剂 (API)基因A构建了植物表达载体。然后通过根癌土壤杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn .)介导将此表达载体上的双抗虫基因转入杂种 741毛白杨 [PopulusalbaL .× (P .davidianaDode +P .simoniiCarr.)×P .tomentosaCarr.]获得了一批抗卡那霉素的转化再生植株。用杨扇舟蛾 (Closteraanachoreta (Fabricius) )进行虫试的结果表明有 3株抗虫杨树 ,其中有1株杨树的叶片可使试虫在 6天内的死亡率达 90 %以上 ,而且存活幼虫的生长发育受到了明显的抑制。PCR检测及基因组DNASouthern杂交分析的结果都表明Bt杀虫蛋白基因和API基因已整合到以上 3株抗虫杨树的基因组中 ,而且表现为单拷贝整合。用Bt毒蛋白抗血清进行滤膜免疫反应及ELISA检测结果表明 3株转基因杨树都有Bt杀虫蛋白的表达 ,表达量约占叶总可溶性蛋白的 0 .0 15 %。这是国内外首次报道用双抗虫基因获得的抗虫 741毛白杨植株。

Transgenic poplars NL-80106 with Bt genes planted in the field were detected by PCR. The results showed that there were the special fragments of Bt genes in 5 transgenic clones with single Bt gene (FB40、FB45、FB56、FB59、FB60) and 4 transgenic clones with two insect-resistant (Bt + CpTI)genes (D9、D18、D27、D32). The leaves from 12 transgenic insect-resistant poplars were analyzed by ELISA and showed that the expressing quantity of Bt insecticidal protein in D18 is 6 754 ng /g fresh leaf (0.090% of plant soluble...

Transgenic poplars NL-80106 with Bt genes planted in the field were detected by PCR. The results showed that there were the special fragments of Bt genes in 5 transgenic clones with single Bt gene (FB40、FB45、FB56、FB59、FB60) and 4 transgenic clones with two insect-resistant (Bt + CpTI)genes (D9、D18、D27、D32). The leaves from 12 transgenic insect-resistant poplars were analyzed by ELISA and showed that the expressing quantity of Bt insecticidal protein in D18 is 6 754 ng /g fresh leaf (0.090% of plant soluble protein), which is the highest in the transgenic clones with two-insect-resistant genes. In the transgenic clones with single Bt gene, the expressing quantity of FB51 is 6 796 ng/g fresh leaf and it amounts to 0.036% of plant soluble protein.

对大田移植的转基因杨树的PCR分析表明 :5个转Bt单基因杨树无性系 (FB4 0、FB4 5、FB5 6、FB5 9、FB6 0 )和 4个转双价基因 (Bt和CpTI)杨树无性系 (D9、D18、D2 7、D32 )均检测到了Bt基因特异片段。在检测的 12个转基因杨树无性系中 ,ELISA结果表明 ,转双价基因杨树无性系中以D18的Bt杀虫蛋白表达量最高 ,Bt杀虫蛋白含量达 6 75 4ng/g鲜叶 ,约占植物可溶性蛋白的 0 0 90 % ;在转Bt单基因杨树无性系中以FB5 1表达量最高 ,达 6 796ng/g鲜叶 ,占植物可溶性蛋白的 0 0 36 %。

 
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