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plaque forming
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  空斑形成
     Methods SD rats were inoculated intranasally and intraperitoneally with 6×106 plaque forming unit(PFU) RSV and LMWH 400 IU/kg.
     方法以6×106空斑形成单位(PFU)RSV鼻腔滴注及腹腔注射,LMWH400IU/kg腹腔注射接种SD大鼠。
短句来源
     Forty BALB/c nude mice were inoculated with Her2 high expressing oophoroma cells of SK OV 3 line and were randomized into 4 equal groups: group A injected with Ad SG Her at the dosage of:2×10 9 plaque forming unit (pfu) through the caudal vein, group B injected with Ad SG Her at the dosage of 1×10 9 pfu, group C injected with Ad SG Her at the dosage of 5×10 8 pfu, and control group.
     治疗组裸鼠尾静脉分别注射不同滴度的Ad SG Her :治疗A组注射 2× 10 9空斑形成单位 ( pfu) /只 ,B组注射 1× 10 9pfu/只 ,C组注射 5× 10 8pfu/只。
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     Results Both CPE and plaque forming of CVB3 were significantly inhibited by GTN and ISDN ( P <0.05).
     结果GTN、ISDN可以明显抑制病毒的细胞病变效应以及空斑形成(P<0.05)。
短句来源
     Replication deficient adenovirus with IFN γ gene (AdCMVIFNγ) 5×10 8 plaque forming unit (pfu) was administrated intratracheally in murine asthmatic model 48 hours before OVA challenging. BAL and lungs were recovered after 48 hour OVA post challenge.
     哮喘模型在OVA激发前 48h ,在其气道内给予带有γ 干扰素的复制缺陷腺病毒 (replication deficientadenoviruswithIFN γgene,AdCMVmIFNγ) 5× 10 8空斑形成单位 (pfu) ,同上在OVA激发 48h后收获其肺泡灌洗液和肺。
短句来源
     The mouse tolerance test showed that the tolerance limit was at least 5×10 11 plaque forming units/kg weight, which was 2500 times higher than the supposed clinical dose.
     同时,小鼠对H1的最大耐受量大于5×1011空斑形成单位kg体重,相当于假定的人的临床剂量的2500倍。
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  空斑形成细胞
     Ceiastrol ip 1, 3mg/kg×5d caused marked decreases of the weights of thymus and of spleen plaque forming cell (PFC) in the mice, caused marked increase in serum complement C3 level, but the level of serum hemolysin and IgG in the mice was not significantly affected.
     雷公藤红素(Celastrol)ip1,3mg/kg×5d能明显减轻小鼠胸腺的重量,降低小鼠脾脏空斑形成细胞数,同时提高血清补体C_3含量,而对小鼠血清溶血素及IgG水平无明显影响。
短句来源
     Results Qubi granule (1 2, 2 4 g·kg -1 , ig, bid×10) improved the phagocytic function of mononuclear phagocytes, inhibited DTH, and increased the number of specific rosette forming cells (SRFC) and plaque forming cells (PFC).
     结果祛痹颗粒剂(12,24g·kg-1,ig,bid×10)能增强小鼠单核吞噬细胞的吞噬功能,抑制DTH,增加脾脏特异性玫瑰花结形成细胞和溶血空斑形成细胞数。
短句来源
     In this report, we observed the effects of Formica fusca L on phagocyte function of peritoneal, macrophage,plaque forming cell of spleen and T lymphocyte subsets of lymphatic node in mice The results showed- the phagocyte function of macrophage, the number of plaque forming, cell, the L3T4 cell, the ratio of L3T4 and Lyt2 cell in the mice of cell the experimental group were increased ( P<0.01~0.001 ) .
     本文观察了黑蚂蚁对小鼠巨噬细胞吞噬功能、空斑形成细胞数及T细胞亚群的影响。 结果表明,实验组小鼠经胃饲蚁粉液(1g/kg)10天后,巨噬细胞吞噬功能、空斑形成细胞数、L_3T_4细胞及L_3T_4/L_(yt_2)比值明显增高,与对照组相比P<0.01~0.001。
短句来源
     At the dose of 50 mg/kg·d ig×5d, AP also caused a significant increase of spleen plaque forming cells ( PFC ) in normal mice.
     当剂量为50mg/kg·d×5d,ig时,它还能显著增加正常小鼠的空斑形成细胞数。
短句来源
     At lower doses, CuB increased the number of peripheral blood T lymphocytes(0.1 mg/kg),the rate of PHA-induced lymphocyte transformation(0.2 mg/kg),the number of plaque forming cells(PFC)of the spleen(0.2 mg/kg)and the level of serum hemolysin(0.4 mg/kg).
     0.2 mg/kg 能提高 PHA 诱导的 T 淋巴细胞转化率和脾脏空斑形成细胞数; 0.4 mg/kg 时血清溶血素水平也明显增加。
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  “plaque forming”译为未确定词的双语例句
     Results Pulmonary titer of mice treated with 0.2, 0.4 mg AS-ODN or 0.8 mg Ribavirin decreased as compared with that of infected control mice, and the plaque forming inhibition ratio was 34.48%, 46.75% and 23.02% respectively.
     结果0.2、0·4mgAS-ODN和0.8mg利巴韦林能降低RSV感染小鼠肺组织病毒滴度,空斑抑制率分别为34.48%、46.75%和23.02%,与感染对照组比较差异显著(P<0·05)。
短句来源
     Virus titer was about 1.4×10 14 pfu/L as determined by plaque forming assay.
     经点杂交筛选阳性空斑 ,纯化后病毒效价达 1.4× 10 14 pfu/L。
短句来源
     Plaque forming cells (PFC) were studied in spleen of mice immunized with sheep red blood cells (SRBC) after X-irradiation with doses of 0.25, 0.50, 1.0,2.0,3.0 and 4.0Gy.
     本实验观察了0. 25、0. 5、1. 0、2. 0、3. 0和4. 0Gy全身照射后3至6 h羊红细胞(SRBC)免疫对小鼠脾脏抗体形成细胞(PFC)的影响。
短句来源
     The plaque forming unit(pfu)of the genomie library is 6×10~4 pfu/ml and has been expanded to 2.6×10~8 pfu/ml,as a permanent source of genomic library of M.leprae.
     重组效率检测表明,阳性重组噬菌体颗粒浓度为6×10~4pfu/ml(噬斑形成单位/ml),且扩增为2.6×10~9pfu/ml,建立了高重组效率和高浓度的 ML DNA 重组噬菌体颗粒悬液,成为 ML 基因组DNA 库的永久来源。
短句来源
     Results:(1)50% inhibitory concentration of glycyrrhizin was 0.56mM by means of plaque forming units.
     结果:(1)空斑减少试验结果显示IC50为0.56mM。
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  plaque forming
BoHV-1 induced expression of major viral glycoproteins at the surface of these cells and produced progeny virus up to 105 plaque forming units per ml.
      
We exposed murine epidermis to 5×104 plaque forming units of HSV-1 and -2, which by day 8 produced inoculation site (primary site) disease scores of 27 and 12.4 respectively, and secondary site disease scores of 29 and 30 respectively.
      
We have evaluated the use of a range of protocols and demonstrate that relatively high titres of 19,000 plaque forming units per millilitre may be recovered after freezing in PBS-sucrose-glutamate-serum buffer (PSGC).
      
For the invasive UC-8 strain, 50-175 plaque forming units of virus per ml was found associated with the blood cells and no virus was detected in the plasma.
      
Inoculation of 2.0×104 plaque forming units (PFU) of wt intraperitoneally into mice resulted in lethal infection accompanying intraperitoneal hemorrhage and pathognomonic changes in the target organs.
      
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A modified method of Jerne's assay was used to determine haemolytic plaque-forming cells (PFC) in mouse spleen. By means of this method we measured the dynamic changes of the immune status in tumor-bearing mice. The tumors used were solid Sarcoma 180 maintained in Tian-jin albino mice, four ascitic tumors including Sarcoma 180 maintained in Tian-jin albino and 615 strain mice, and Hepatoma and Ehrlich carcinoma maintained in C57BL mice. The results. showed that spleen PFC decreased as the tumors...

A modified method of Jerne's assay was used to determine haemolytic plaque-forming cells (PFC) in mouse spleen. By means of this method we measured the dynamic changes of the immune status in tumor-bearing mice. The tumors used were solid Sarcoma 180 maintained in Tian-jin albino mice, four ascitic tumors including Sarcoma 180 maintained in Tian-jin albino and 615 strain mice, and Hepatoma and Ehrlich carcinoma maintained in C57BL mice. The results. showed that spleen PFC decreased as the tumors progressed, and was hardly detected when the tumors were far advanced. The tumor-bearing mice commonly had striking splenomegaly with. decrease in number of small lymphocytes and. increase of 'immature' plasma cells, which might explain the poor immune responses of tumor-bearing mice.

本文应用改良的Jerne氏方法测定小鼠脾脏溶血空斑形成细胞。用此方法测定一株实体瘤(在津白小鼠上移植之肉瘤180)和四株腹水瘤(津白小鼠上移植之肉瘤180,615小鼠上移植之肉瘤180,以及C57BL小鼠上移植之肝癌和艾氏癌)带瘤动物免疫状态的动态变化。结果表明,随着肿瘤进行性生长,小鼠脾脏溶血空斑形成细胞逐渐减少,到晚期几乎不能测得。带瘤小鼠的脾脏普遍增大,病理组织学观察提示带瘤小鼠溶血空斑反应之低下与其脾脏的病理学改变相关。

It has been shown by haemolytic plaque-forming cell assay that there is an immunosuppressive factor(s) with high activity in the cell-free aseitic fluid of Sarcoma 130 maintained in 615 strain mice. The suppression is dose-dependent and becomes apparent only when the aseitic fluid is administered before immunization with sheep red blood cells. The immunosuppressive factor (s) seems partially heat-labile. The serum of the same tumor-bearing mice also possesses a strong immunosuppressive activity....

It has been shown by haemolytic plaque-forming cell assay that there is an immunosuppressive factor(s) with high activity in the cell-free aseitic fluid of Sarcoma 130 maintained in 615 strain mice. The suppression is dose-dependent and becomes apparent only when the aseitic fluid is administered before immunization with sheep red blood cells. The immunosuppressive factor (s) seems partially heat-labile. The serum of the same tumor-bearing mice also possesses a strong immunosuppressive activity. Furthermore, similar immunosuppressions were observed in normal recipient mice treated with cell-free aseitic fluids and sera of mice bearing some other strains of tumor including Hepatoma and Ehrlich carcinoma maintained in C57BL mice and Sarcoma 180 maintained in Tian-jin albino mice. Histopathologic changes of spleens induced by the cell-free aseitic fluid resembled those observed in spleens of tumor-bearing mice.

用溶血空斑试验证明,在615小鼠上移植的肉瘤180的无细胞腹水液中存在着高活性的免疫抑制因子。腹水液的免疫抑制作用与剂量相关,且仅在绵羊红血球抗原致敏前注射才能显示。此免疫抑制因子具有部分热不稳定的性质。肉瘤180带瘤小鼠的血清也有强免疫抑制活性。在C57BL小鼠上移植的肝癌和艾氏癌,在津白Ⅰ号小鼠上移植的另一株肉瘤180的腹水液和带瘤动物血清也有类似的免疫抑制作用。肿瘤无细胞腹水液引起的小鼠脾脏病理组织学变化与带瘤动物脾脏所见相类似。

An improved plaque-forming assay for the detection of antibody-forming cells was reported in this paper.Onthe basis of Cunningham's slide cham-ber,improvements have been made inthe making of the chambers and thecounting of the plaques.Cover glassstripes in place of double sided self-adhesive tape were put between twoslides.The edges were sealed withparaffin (Fig.1),The chambers thusformed were filled up with a mixture,including spleen cells,SRBC,comple-ments,etc.On counting,a cover glasswith...

An improved plaque-forming assay for the detection of antibody-forming cells was reported in this paper.Onthe basis of Cunningham's slide cham-ber,improvements have been made inthe making of the chambers and thecounting of the plaques.Cover glassstripes in place of double sided self-adhesive tape were put between twoslides.The edges were sealed withparaffin (Fig.1),The chambers thusformed were filled up with a mixture,including spleen cells,SRBC,comple-ments,etc.On counting,a cover glasswith counting grid was put on thesurface of the chamber.According tothe area of the cover glass,the heightof the chamber,and the concentrationof spleen cells used,the number ofPFC in spleens can be calculated.Through the study of the kineticsof anti-DNP antibody-forming cells ofrats,it was shown that the methoddescribed here was simple and reliable.Methods of coupling hapten to SRBCand some influences of complementsand anti-IgG antiserum in this exper-iment were discussed.

本文报道了一种改良的检出抗体生成细胞的溶血空斑技术。在Cunningham 玻片小室法基础上,对玻片小室的制作,空斑的计数等方面进行了改进。通过对大鼠抗DNP 半抗原的抗体生成细胞的动力学检测,表明本文所述方法是简便可行的。此外对半抗原与SRBC 的连结和影响实验的一些因素——补体、抗TgG 抗血清等进行了探讨。

 
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