Objective To evaluate the protective effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) on acute hepatic failure induced by galactosamine (D-GalN) and lipopolysaccharide (LPS) in mice, and to explore its mechanism.
目的:观察重组人粒细胞集落刺激因子(recombinant human granulocyte colony stimulating factor;rhG-CSF)对D-氨基半乳糖(D-galactosamine,D-GalN)加脂多糖(lipopolysaccharide,LPS)联合诱导的小鼠急性肝衰竭的保护作用,并探讨其机制。
Objctive:It is surveyed whether the recombinant adenovirus vector pAdeno-X-siFas 1+siFas 2 expressing two Fas-targeted short hairpin RNAs(shRNA) could supress the overexpression of Fas induced by LPS/D-GalN in BALB/c mouse liver.
Methods: Mice were pretreated with LMFO(50?100?150 mg/kg ig respectively, 10 days),and then 0.2 % CCl 4 10 ml/kg ig and D-GalN(600 mg/kg)+LPS(lipopolysaccharide,1 μg/kg) ig respectively in two model groups to induce liver injury.
Methods: by means of intravenous injection of endotoxin 1.5 mg/kg and abdominal injection of D-GaLN 100 mg/kg, models of endotoxic shock were made, and INOS expression in brain was detected by immunohistochemical method.
AIM: To study the effects of matrine (Mat) on lipopolysaccharides (LPS)-induced fatal hepatitis in D-galactosamine (D-GalN)-sensitized mice and tumor necrosis factor ( TNF ) release from peritoneal macrophages (PM0).
Objective To investigate the effect of allogeneic hepatocyte transplantation (HcT) plus augmenter of liver regenration (ALR) intraperitoneally on acute hepatic failure (AHF) induced by D-galactosamine (D-gal) in rats.
目的探讨同种异体肝细胞腹腔移植联合肝再生增强因子(augmenter of liver regenration,ALR)对D-氨基半乳糖(D-gal)诱导的急性肝功能衰竭大鼠的治疗作用。
Aim To explore the possibility of LALF in the improvement of the survival of D galactosamine sensitized mice challenged by LPS and the role of LALF on the production of NO and IL 10 of murine peritoneal macrophages stimulated by LPS.
Methods Hepatic injury model were made by having injected D galactosamine(D GaLN) into mouse abdominal cavity ,then to observe a series of effect of normal bacteria in the intestinal tract, the activity of organize diamine oxidase(DAO),the content of blood endotoxin(LPS) and liver pathology brought by chinese herbs.
方法 小白鼠腹腔注射D-氨基半乳糖( D- Ga L N) ,制备肝损伤模型,观察中药对肠道正常细菌,组织二胺氧化酶( DAO)活性,血内毒素( L PS)含量的影响及肝脏病理学影响。
In mice sensitized withd-galactosamine (d-GalN), high doses of bacterial DNA from either gram-positive or gram-negative pathogens induce a lethal cytokine syndrome (lethal shock).
Acute liver injury was induced by a single administration of D-galactosamine (D-Galn) and lipopolysaccharide (LPS) or carbon tetrachloride (CCl4).
Non-parenchymal liver cells from rats with D-Galn/LPS-induced acute liver injury produced prominantly leukotriene B4 and 5-hydroxy-arachidonic acid which were hardly synthesized by the normal rat liver.
The hepatoprotective effect of LMWF was studied using two acute liver injury mice models induced by carbon tetrachloride (CCl4) and D-galactosamine (D-GalN).
Moreover, administration of LMWF significantly inhibited the elevation of glutamate pyruvate transaminase induced by CCl4 and D-GalN in mice.