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copper binding protein
相关语句
  铜结合蛋白质
     1. We isolated . a novel copper binding protein ( 58 KD).
     1.经上述的处理和分析,大鼠肝脏溶酶体中铜结合蛋白质(分子量58KD)与哺乳动物蛋白质无同源性。
短句来源
     Objective: To isolate copper binding protein from rat liver lysosomal fraction, and to analyze its amino acid sequences.
     目的:分离大鼠肝脏溶酶体中铜结合蛋白质,并分析其氨基酸序列。
短句来源
  结合蛋白
     Isolation of a Novel Copper Binding Protein from Rat Lysosomal Fraction
     大鼠肝脏溶酶体中新的铜结合蛋白的分离和纯化
短句来源
  铜结合蛋白
     Isolation of a Novel Copper Binding Protein from Rat Lysosomal Fraction
     大鼠肝脏溶酶体中新的铜结合蛋白的分离和纯化
短句来源
  “copper binding protein”译为未确定词的双语例句
     2. Purification copper binding protein using G - 25 column and copper chelating column.
     2.通过G-25层析和铜结合层析纯化大鼠肝脏溶酶体中与铜具有亲和性的蛋白质。
短句来源
     Plastocyanin is a copper binding protein, which play an important role in electron transmission.
     质体蓝素是一种含铜离子的蛋白,其在植物的电子传递过程中起着重要作用。
短句来源
     3. Analysis of copper binding protein by SDS - PAGE, three main protein bands observed. The main bands were digested by lysyl endopeptidase and isolate different peptides by HPLC.
     3.SDS-PAGE分析得到3条主要蛋白带,剪下这三条带进行胶内赖氨酸内切酶的消化,通过高效液相色谱分离肽段,选择性进行肽段的氨基酸序列测定。
短句来源
     2. We use the novel copper binding protein as antigen, prepared rabbit anti rat copper binding protein polyclonal antibody.
     2.免疫家免得到了兔抗大鼠钢结合蛋白质的多克隆抗体。 讨 论
短句来源
     Results:We isolated copper binding protein from rat liver lysomal fraction;
     结果:G25和铜结合液相层析得到溶酶体中蛋白质成分。
短句来源

 

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      copper binding protein
    Indeed, S100A13 is a copper binding protein able to enhance the export of FGF1 in response to stress in vitro and to induce the formation of a multiprotein aggregate responsible for FGF1 release.
          
    To identify the copper binding protein, we developed a fractionation procedure which included solubilisation of PS II particles followed by precipitation with polyethylene glycol.
          
    The humanPKR gene was fused to the promoter of theArabidopsis blue copper binding protein gene(BCB) that is induced rapidly in response to wounding.
          
    Reversion of hypertrophic cardiomyopathy in a patient with deficiency of the mitochondrial copper binding protein Sco2: Is there
          
    Characterization of the copper(II) binding site in the pink copper binding protein CusF by electron paramagnetic resonance spect
          
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    Ssveral experiments have been done to demonstrate molybdate sulphur and their compound-trithiomolybdate could affect the metabolism of copper bound proteins in liver cytosol, especially copper on the metallothioncin. A possibility is that molybdate strip copper from metallothionein in the cytosol of liver of cattle. Copper stripped either moved out of cells as a small molecullar compound in vivo, or combined to a large molecullar proteins in cytosol in vitro. Meanwhile, metallothionein...

    Ssveral experiments have been done to demonstrate molybdate sulphur and their compound-trithiomolybdate could affect the metabolism of copper bound proteins in liver cytosol, especially copper on the metallothioncin. A possibility is that molybdate strip copper from metallothionein in the cytosol of liver of cattle. Copper stripped either moved out of cells as a small molecullar compound in vivo, or combined to a large molecullar proteins in cytosol in vitro. Meanwhile, metallothionein may capture copper from other source, for example, a large molecullar copper bound proteins. The peak of metallothionein appcars when thiomolybdate excreted from cells. Dithiothretol can stablize the combination of copper to metallothionein. There is no obvious effect on the combination of copper to the proteins when samples were stored in -20℃ for five weeks.

    以8头公犊为材料,通过体内和体外试验证明硫、钼及其化合物三硫钼酸盐被吸收或直接注入血液进入肝脏后,对肝内铜代谢的干扰作用主要是促使铜与咪噻宁蛋白质分离。分离后的铜成为小分子化合物离开肝细胞,而在体外试验中,还有部分铜转移到分子量较大的蛋白质上。一旦硫钼酸盐从肝内排泄后,体内铜很快与咪噻宁重新结合。缓冲液中二硫苏糖醇可使铜与咪噻宁结合保持恒定,样品在冰冻情况下保存5周,对咪噻宁与铜结合作用无明显影响。

    To inquire into the relationship between iron,copper,HBV and hepatocelluar carcinoma(HCC),we collected 70 storing Paraffin blocks of patients with cirrhosis and HCC and 10 normal controlblocks.and surveyed them with methods of cytochemistry and image analysis technique (IAT).The relativecontent of hepatic iron was significantly higher in positive HBsAg group than in negative group (P<0. 05). Itindicated that there was a close relationship between HBV and iron. Distribution of iron granule.was even innormal...

    To inquire into the relationship between iron,copper,HBV and hepatocelluar carcinoma(HCC),we collected 70 storing Paraffin blocks of patients with cirrhosis and HCC and 10 normal controlblocks.and surveyed them with methods of cytochemistry and image analysis technique (IAT).The relativecontent of hepatic iron was significantly higher in positive HBsAg group than in negative group (P<0. 05). Itindicated that there was a close relationship between HBV and iron. Distribution of iron granule.was even innormal liver. exttemely uneven in cirrhosis and always negative in HCC.We found a siderotic macroregenerative nodule(MRN) within which there was an iron-negative lesion of HCC. We considered that the appearance of "nodule within nodule" was the early stage of canceration and iron acted as an improving factor toHCC. In addition. we found that granules of copper and copper-binding protein (CBP) were related to inflammation and fibrosis and appeared mainly in well-differentiated HCC. It is concluded that the effects ofcopper were different from those of iron to HCC.

    为探讨铁、铜、乙肝病毒与肝癌的关系,利用组化染色及图像分析技术对贮存的70例肝癌、肝硬化标本蜡块及10例正常对照标本进行了观测分析.HBsAg阳性组肝铁含量显著高于阴性组(P<0.05).提示HBV与铁关系密切.正常肝铁分布均匀;肝硬化铁分布参差不一,与肝细胞增生有关;肝癌细胞内无铁颗粒.与周围肝细胞形成了明显的界线.有铁聚集的肝再生巨结节(MRN)中出现的“灶中灶”结构可能为肝硬化癌变的初始阶段表现,铁可能有促癌作用.铜颗粒的出现与炎性反应、纤维组织增生等有关,常出现于分化较好的肝癌细胞内,其作用不同于铁.

    Objective: To isolate copper binding protein from rat liver lysosomal fraction, and to analyze its amino acid sequences. Methods: G25-chromatography, copper-chelating chromatography, high performance liquid chromatography, amino acid sequencing were used. Results:We isolated copper binding protein from rat liver lysomal fraction; Two bands of protein of 58kDa and 40kDa were observed. Amino acid sequence was A-F-Q-I-L-Y-L-T-H-N-L. Homology search showed the protein was a novel protein....

    Objective: To isolate copper binding protein from rat liver lysosomal fraction, and to analyze its amino acid sequences. Methods: G25-chromatography, copper-chelating chromatography, high performance liquid chromatography, amino acid sequencing were used. Results:We isolated copper binding protein from rat liver lysomal fraction; Two bands of protein of 58kDa and 40kDa were observed. Amino acid sequence was A-F-Q-I-L-Y-L-T-H-N-L. Homology search showed the protein was a novel protein. Conclusion: We isolated a novel copper binding protein(58kDa).

    目的:分离大鼠肝脏溶酶体中铜结合蛋白质,并分析其氨基酸序列。方法:G25液相层析,铜结合层析,高效液相色谱,氨基酸序列测定。结果:G25和铜结合液相层析得到溶酶体中蛋白质成分。SDS-PAGE电泳分析铜结合蛋白质,得到两条主要蛋白带,这些蛋白质的分子量分别为 58kDa, 40kDa。两种主要蛋白质的氨基酸序列测定Band1(分子量 58kDa):AFQILYLTHNL。同源性分析表明蛋白质与哺乳动物蛋白质无同源性。结论:从大鼠肝脏溶酶体中分离纯化得到的蛋白质是一种新的蛋白质。

     
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