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rabies virus glycoprotein
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  狂犬病毒糖蛋白
     Isolation of Vaccinia Virus Recombinants Expressing Rabies Virus Glycoprotein by Lac Gene
     用Lac基因筛选表达狂犬病毒糖蛋白的重组痘苗病毒
短句来源
     he recombinant vaccinia virus(VVM11KRG strain)contains rabies virus glycoprotein gene(RVGG) which was inserted into the Hind ⅢM fragment of vaccinia virus(V.V)genome.
     对表达狂犬病毒糖蛋白的重组痘苗病毒VVM11KRG株生物学性质进行了研究,该重组病毒的特点是:(1)糖蛋白基因插入到痘苗病毒天坛株基因组HindⅢM片段中;
短句来源
     Methods According to the strategy of transient dominant selection, the rabies virus nucleoprotein (RN) gene was introduced into recombinant vaccinia virus(VTKRG) which expresses rabies virus glycoprotein (RG) gene in the TK locus by homologous recombinant.
     方法 利用TK区表达狂犬病毒糖蛋白 (RG)的重组痘苗病毒作为亲本株 ,通过两步同源重组 ,删除痘苗病毒基因组CK片段间与毒力和宿主范围相关的核酸片段 ,同时将狂犬病毒核蛋白 (RN)基因插入C K片段之间 ,获得了含有RG基因和RN基因的非复制型重组痘苗病毒VTKRGΔCKLacZRN。
短句来源
     Construction of recombinant plasmid carrier including the principal immunocompetent domains of rabies virus glycoprotein and nucleoprotein
     狂犬病毒糖蛋白和核蛋白主要免疫活性位点的质粒载体构建
短句来源
     GOMPARATIVE STUDIES OF TWO METHODS TRANSFECTING RABIES VIRUS GLYCOPROTEIN GENE
     两种方法转染狂犬病毒糖蛋白基因的比较研究
短句来源
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  狂犬病病毒糖蛋白
     The rabies virus glycoprotein gene was cloned into the Bam HI site ofhigh level expressing vectors-pET-17b and pET-17b2. And pET-17b2 was reconstructed from pET-17b by Nde Ⅰ- Sac Ⅱ Ⅰ. Two recombinant plasmids,pET-17RVgpand pET-17b2RVgp were gotten. They were transferred into expressing host cells E。
     将狂犬病病毒糖蛋白(RVgp)基因BglⅡ片段(1675bp)分别正向插入到原核高效表达载体pET-17b和pET-17b2(用SacⅠ-NdeⅠ缺失掉pET-17b60bp含起始密码子ATG小片段)的BamHⅠ切点,构建重组质粒pET-17bRVgp和pET-17b2RVgp。
短句来源
     Transgenic Mice Expressing Rabies Virus Glycoprotein Developed Immune Tolerance to Rabies Viruses
     狂犬病病毒糖蛋白转基因小鼠对该病毒形成免疫耐受
短句来源
     Expression of Rabies Virus Glycoprotein Gene in E. coli
     狂犬病病毒糖蛋白基因在原核细胞中的表达
短句来源
     Cloning of Enrich Epitope of Rabies Virus Glycoprotein Gene and Its Expression in Prokaryotic Cells
     狂犬病病毒糖蛋白富集表位基因克隆及其在原核系统中的表达
短句来源
     Construction of Recombinant Canine Adenovirus Type-2 Expressing Rabies Virus Glycoprotein
     表达狂犬病病毒糖蛋白的重组犬2型腺病毒的构建
短句来源
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  狂犬病病毒糖蛋白基因
     Expression of Rabies Virus Glycoprotein Gene in E. coli
     狂犬病病毒糖蛋白基因在原核细胞中的表达
短句来源
     With flank sequence of HA gene of vaccinia virus, four eucaryotic expression plasmids containing cDNA of rabies virus glycoprotein gene under the control of different hybrid promoters of pox virus were constructed and transfected into BHK 21 cells infected with wild type vaccinia virus.
     以血凝素(HA)基因为侧翼,将狂犬病病毒糖蛋白基因cDNA置于不同类型的痘苗病毒复合启动子下游,构建了4种表达质粒。
短句来源
  “rabies virus glycoprotein”译为未确定词的双语例句
     A recombinant plasmid comprising adenovirus genome with rabies virus glycoprotein gene in E1 region was constructed by homologous recombination.
     利用大肠杆菌内质粒间同源重组的方法,将狂犬病病毒G基因插入腺病毒基因组的E1基因区,构建了带有狂犬病病毒G基因的重组腺病毒质粒。
短句来源
     To assay the immunological effect of rabies virus glycoprotein and nucleoprotein bivalent DNA vaccine and IL 18 on dogs.
     目的 检测狂犬病病毒糖 /核蛋白“二价”基因疫苗及IL - 18在犬体内的免疫效果 ,并确定不同包裹剂对基因疫苗免疫效果的影响。
短句来源
     Evaluation on the biosafety for the rabies virus glycoprotein/nucleoprotein DNA vaccine in dogs
     狂犬病病毒糖/核蛋白基因疫苗中间试验生物安全评价
短句来源
     Immunological experiment on dogs by Rabies virus glycoprotein/ nucleoprotein DNA vaccine and IL-18
     狂犬病病毒糖/核蛋白基因疫苗和IL-18在犬的免疫试验研究
短句来源
     HA gene of vaccinia virus is an unnecessary region for virus replication It was used as a flank to construct 12 recombinant vaccinia viruses,in which HIV-1 gag gene,HIV-1  gag-env  chimeric gene and Rabies virus glycoprotein gene were inserted into the downsteam of various types of pox virus promoter.
     以痘苗病毒HA基因为侧翼,在多种类型痘病毒)启动子的下游,插入HIV-1的gag基因及其上游序列、或HIV-1gag-env嵌合基因、或狂犬病病毒的糖蛋白基因,从而构建了12株重组痘苗病毒。
短句来源
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  rabies virus glycoprotein
Inhibition of rabies virus replication was detected in cell culture using an ELISA for detection of rabies virus glycoprotein expression on the cell surface and immunofluorescence for detection of intracellular rabies virus N expression.
      
Twenty sheep were divided into groups and inoculated by various routes with recombinant raccoon poxvirus expressing the CVS rabies virus glycoprotein (rRCNV-G) or with raccoon poxvirus (RCNV).
      
Raccoon poxvirus rabies virus glycoprotein recombinant vaccine in sheep
      
Sequence homology between HIV gp120, rabies virus glycoprotein, and snake venom neurotoxins
      
A region of the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp120 has been claimed previously to be homologous to parts of snake venom neurotoxins and rabies virus glycoprotein ("the neurotoxic loop").
      
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in this paper, we reported the results obtained from two trans feetingmethods of Rabies Virus glycoprotein. Comparative studies apparently showed thatLipofectingTM Reagent Method is superior to Calsium Phosphate PrecipitateMethod.

两种方法转染狂犬病毒糖蛋白基因的比较研究李萍萍,吴柏春(武汉生物制品研究所)(华中师范大学昆虫学研究所)关键词转染;Lipofectin ̄TMReagent;重组病毒中图分类号Q785转染是分子生物学中常用的重要技术之一.目前较为普遍使用的转染方法有...

A vaccinia recombinant containing the glycoprotein (G) gene of Chinese Rabies Strain (5aG), which was controlled under the vaccinia virus promoter p11, was constructed by inserting the G gene into the TK region of vaccinia virus.Through indirect immunofluorescence and western blot assays, it was shown that the recombinant VVaG can successfully express the rabies virus glycoprotein, and its molecular weight is was shown that the recombinant VVaG can induce rabies virus neutralizing antibodies...

A vaccinia recombinant containing the glycoprotein (G) gene of Chinese Rabies Strain (5aG), which was controlled under the vaccinia virus promoter p11, was constructed by inserting the G gene into the TK region of vaccinia virus.Through indirect immunofluorescence and western blot assays, it was shown that the recombinant VVaG can successfully express the rabies virus glycoprotein, and its molecular weight is was shown that the recombinant VVaG can induce rabies virus neutralizing antibodies (VNA) in all immunized mice and protect them against lethal challege of rabies virus aG and CVS strain intracerebally,and the highest titer of rabies VNA Can reach to 4169 on the 21th day post immunization.

将克隆到的中国狂犬病毒疫苗株(5aG)的糖蛋白基因重组到痘苗病毒TK区,并在痘苗病毒P11启动子的控制下,构建了狂犬-痘苗重组病毒(VVaG)。经间接免疫荧光和Western免疫印染证明,重组病毒VVaG能良好地表达狂犬病毒糖蛋白,其分子量约为6600。用VVaG免疫小鼠,7d便可诱生较高的狂犬病毒中和抗体,21d达4169,并能100%保护狂犬病毒本毒株和国际标准攻击毒(CVS)的致死量攻击。

Expressing vector, pMT010/A+ -Rgp, was constructed by cloning rabies virus glycoprotein( Rgp)cDNA into downstream of the metallothionein promoter of pMT010/A+(ovine).The 2.76 kb Eco R I +Sal I fragments containing Rgp cDNA of pMT010/A+-Rgp were recovered and purified for microinjecting into male pronuclei of fertilized mouse eggs.Of 44 mice developed from these eggs, nine carried the gene of in- terest,which was identified by PCR, Southern hybridization and in situ hybridization. All of transgenic mice...

Expressing vector, pMT010/A+ -Rgp, was constructed by cloning rabies virus glycoprotein( Rgp)cDNA into downstream of the metallothionein promoter of pMT010/A+(ovine).The 2.76 kb Eco R I +Sal I fragments containing Rgp cDNA of pMT010/A+-Rgp were recovered and purified for microinjecting into male pronuclei of fertilized mouse eggs.Of 44 mice developed from these eggs, nine carried the gene of in- terest,which was identified by PCR, Southern hybridization and in situ hybridization. All of transgenic mice transmit their transgene to 50%of their offsprings indicative of Mendelian inheritance, Six out of 9 lines were designated as TgN (oMT- Rgp)1 Lge,TgN(oMT-Rgp)2Lge…TgN(oMT-Rgp)6Lge.

将狂犬病病毒糖蛋白cDVABglⅡ(1.67kb)片段正向插入pMT010/A+BamHⅠ切点,构建重组质粒pMT010/A+-Rgp,用EcoRⅠ+SalⅠ对pMT010/A+-Rgp进行双酶切后,回收含有狂犬病病毒糖蛋白cDNA和绵羊MT启动子的2.76kb片段,通过显微注射技术将该片段注入小鼠单细胞受精卵雄前核内,在进行胚胎移植后,获得44只小鼠,经PCR、South-ern杂交及原位杂交检测,证明有9只携带有目的基因。目的基因(即转基因)按孟德尔特性遗传给其后代,由此相应建立了9个转基因鼠系,对其中6个鼠系分别命名为TgN(oMT-Rgp)ILge、TgN(oMT-Rgp)2Lge……TgN(oMT-Rgp)6Lge。

 
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