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hepatitis
相关语句
  肝炎
    Study of Genes Expressed Differentially in Immunocyte of Patients with Fulminant Hepatitis B
    重型乙型肝炎免疫细胞差异表达基因研究
短句来源
    1. The Experimental Study of rALR on Immunologic Response Induced by Exogenous Antigen in Rat 2. Analysis on Cause of Death in 115 Patients with Hepatic Failure Induced by Viral Hepatitis 3. The Primary Clinical Study of Peginterferon Alfa-2a in the Treatment the Patients with HBeAg-Positive Chronic Hepatitis B and with Drug Fast to Lamivudine
    1.肝再生增强因子对外原性抗原引起机体免疫应答影响的初步研究 2.病毒性肝炎肝衰竭患者死亡原因分析 3.聚乙二醇化干扰素α-2a治疗HBeAg阳性的慢性乙型肝炎及其应用于拉米夫定耐药的初步临床观察
短句来源
    ASSESSMENT OF NK ACTIVITY IN PERIPHERAL BLOOD WITH ~(125)I-UdR LABELLED K 562 CELLS IN 29 CASES OF VIRAL HEPATITIS
    用~(125)IUdR标记K562细胞法检测乙型肝炎患者外周血中的NK细胞活性
短句来源
    A Study of Sister Chromatid Exchange (SCE) in Peripheral Lymphocytes of Hepatitis B Patients with HBsAg Positive and Their Children
    HBsAg阳性乙型肝炎患者及其子女外周血淋巴细胞姐妹染色单体互换的研究
短句来源
    PRELIMINARY STUDY OF T CELL SUBSETS AND T4/T8 DOUBLE LABELED CELLS IN CHRONIC HEPATITIS B PATIENTS
    慢性乙型肝炎患者外周血T细胞亚群及T_4/T_8双标志细胞的初步研究
短句来源
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  病毒性肝炎
    The Diagnostic Technique and Clinical Application of Gene Chip of Hepatitis Virus
    病毒性肝炎基因芯片诊断技术研究和临床应用
短句来源
    1. The Experimental Study of rALR on Immunologic Response Induced by Exogenous Antigen in Rat 2. Analysis on Cause of Death in 115 Patients with Hepatic Failure Induced by Viral Hepatitis 3. The Primary Clinical Study of Peginterferon Alfa-2a in the Treatment the Patients with HBeAg-Positive Chronic Hepatitis B and with Drug Fast to Lamivudine
    1.肝再生增强因子对外原性抗原引起机体免疫应答影响的初步研究 2.病毒性肝炎肝衰竭患者死亡原因分析 3.聚乙二醇化干扰素α-2a治疗HBeAg阳性的慢性乙型肝炎及其应用于拉米夫定耐药的初步临床观察
短句来源
    Serological and Histopathological Studies on Viral Hepatitis of the Citellus dauricus
    达乌尔黄鼠病毒性肝炎的检测及组织病理学研究
短句来源
    Advances in the Research on Animal Models of Human Hepatitis
    病毒性肝炎动物模型的研究
短句来源
    Fas antigen and fas ligand expression in liver tissues in patients with acute hepatitis B
    急性乙型病毒性肝炎肝组织中Fas抗原和Fas配体的表达
短句来源
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  “hepatitis”译为未确定词的双语例句
    Studies on Establishment and Characteristics of Hepatitis G Virus Transgenic Mouse Models
    庚型肝炎病毒转基因小鼠模型的建立及其特性研究
短句来源
    Study on the Relationship between Hepatitis B Virus and Hepatocyte Apoptosis
    乙型肝炎病毒感染与肝细胞凋亡的关系研究
短句来源
    Expression and Immunoactivity of HBsAg (hepatitis B Virus Surface Antigen) and HEV-NE2 (hepatitis E Virus ORF2 Gene) in Transgenic Tomato
    乙肝病毒表面抗原(HBsAg)、戊肝病毒ORF2基因片段NE_2在番茄中的表达及免疫活性
短句来源
    Studies on Relationship between Pathogenicity and Intracellular Signaling Initiated by Hepatitis C Virus E2 Protein
    丙型肝炎病毒E2蛋白引发细胞跨膜信号转导异常与致病性研究
短句来源
    Expression, Purification, and Applications of Hepatitis C Virus (HCV) Envelope Proteins
    丙型肝炎病毒被膜蛋白的表达、纯化与应用研究
短句来源
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  hepatitis
HIV is the most significant risk factor for many opportunistic infections like tuberculosis, hepatitis, bacterial infections etc.
      
Fulminant hepatitis is fatal in most cases and timely liver transplantation is the only effective treatment.
      
This study evaluates the survival outcomes of patients who underwent living-donor liver transplantation (LDLT) using right lobe liver grafts for fulminant liver failure due to hepatitis B infection.
      
In general, it is extremely difficult to treat fulminant hepatitis by conservative regimen, particularly, in cases with rapid progression.
      
Emergency adult living-donor liver transplantation is an effective treatment for fulminant hepatitis patients and is relatively safe for donors.
      
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PLO/PRF/5 hepatoma cell line (PLO) adapted to Eagles' medium supplemented with 10% of inactivated calf serum in our laboratory was successfully passaged, and confluent growth was generally observed on the third day after seeding. The production of hepatitis B surface antigen(HBsAg)in tissue culture fluid was serially determined by solid phase radioimmunoassay. Prolonged incubation of culture in growth or maintenance media resulted in a progressive increase in HBsAg, which was maximum around 18 days, and...

PLO/PRF/5 hepatoma cell line (PLO) adapted to Eagles' medium supplemented with 10% of inactivated calf serum in our laboratory was successfully passaged, and confluent growth was generally observed on the third day after seeding. The production of hepatitis B surface antigen(HBsAg)in tissue culture fluid was serially determined by solid phase radioimmunoassay. Prolonged incubation of culture in growth or maintenance media resulted in a progressive increase in HBsAg, which was maximum around 18 days, and then declined gradually. Compared with highly purified HBsAg prepared from sera of carriers, maximum production of HBsAg from PLO cultures collected between 14-18 days was about 200ng/ml/105 cells. The increase of HBsAg production was paralleled by cytological changes, in which there were degenerations in lysosomes and mitochondria, fusion of cells, and increase in the number of detached and non-viable rounded cells. These were in agreement with the suggestion that HBsAg production is cell cycle related to and representing a terminal event. Depletion of calf serum from media within four days of culture had no significant effect on the production of HBsAg, but after four days cells were found to be detached from flaska, followed by decreased production of HBsAg. When polysaooharides of Eadix Astragali Seu Hedysari (BAH), a Chinese herb which has been shown to prolong the life-span of human embryo lung diploid cell strain was added to culture media, the life-span of PLC, however, was not prolonged, nor was the production of HBsAg enhanced significantly.

PLC/PRF/5肝癌细胞系(PLC)经适应于本实验室含10%小牛血清的Eagles’营养液(csEagles’液),传代3天后即可长成单层。应用固相放射免疫测定法(SPRIA)动态检测产生乙型肝炎表面抗原(HBsAg)量,发观随培养天数延长,HBsAg产量增多,到18天左右达高峰。与纯化HBsAg此较,最高产量(14~18天)约为200ng/ml/10~5细胞。HBsAg产量的增多与PLC细胞出现溶酶体及线粒体退变、细胞融合及脱落死亡圆细胞数增多呈平行关系。这些现象与认为产生HBsAg是PLC生长周期末期活动的看法相符。自营养液中去除小牛血清,则细胞仅能短期维持,第4天起开始脱落,但4天内对HBsAg产量无显著影响。加入黄芪多糖培养,未能延长细胞生长寿命或明显提高HBsAg产量。

Two plasmids containing a single copy of hepatitis B virus (HBV) genome (ayw or adw) have been introduced into mouse Ltk-cells by calcium precepitation and cotransformation with cloned herpes simplex virus thymidine kinase (tk) gene. Under the selective pressure of HAT medium, 6 cloned tk~+ cell lines were found to excrete specific HBsAg in cell culture medium. Two cell lines, pA-3 and pC-3 were selected for further studies. These cell lines were found to be composed of HBsAg positive and HBsAg negative...

Two plasmids containing a single copy of hepatitis B virus (HBV) genome (ayw or adw) have been introduced into mouse Ltk-cells by calcium precepitation and cotransformation with cloned herpes simplex virus thymidine kinase (tk) gene. Under the selective pressure of HAT medium, 6 cloned tk~+ cell lines were found to excrete specific HBsAg in cell culture medium. Two cell lines, pA-3 and pC-3 were selected for further studies. These cell lines were found to be composed of HBsAg positive and HBsAg negative cells on subcloning. The pC-3-1 subline cells obtained from pC-3 cell line by subcloning retained their ability to excrete HBsAg after 16o days passage. This phenomenon shows. that the subcloning procedure is essential for obtaining stable cell lines continously excreting HBsAg. The HBsAg excreted into the cell culture medium was shown by immunoelectromicroscopy to be composed of particles about 22nm diameters, being similar to those found in hu- man serum. Since in our experiments, cotransformation was successful with a single copied HBV-DNA, it seems to indicate that two copies of HBV-DNA in tandem are not essential.

用共转化技术分别将两种亚型的单拷贝HBV-DNA重组质粒与HSV-tk基因一起导入Ltk细胞获得成功。6系克隆化的tk~+细胞分泌HBsAg。分泌量较高的pA-3及PC-3两系细胞分泌物为特异性HBsAg,并含有约22nm大小的颗粒性HBsAg。初次细胞克隆HBsAg表达不稳定,再次克隆获得PC-3-1亚系传代160天仍表达HBsAg,说明再次克隆是选择HBsAg表达较稳定的细胞系的必要手段。单拷贝HBV-DNA转化成功,似说明首尾相连的双拷贝HBV-DNA并非必需。

The entire observation of biological characteristics on the cultured cells of five human hepatoma (hepatocellular carcinoma) cell lines (PLC/PRF/5, MAH, BEL-7402, QGY-7703, SMMC-7721) was completed. The scope of observation included cell morphology, growth, frozen, preservation in liquid nitrogen and recovery, chromosome counts, and detection of special material (e.g. AFP, HBsAg). The results of our observation confirmed that the cells are of epithelial origin malignant in nature and are able to produce and...

The entire observation of biological characteristics on the cultured cells of five human hepatoma (hepatocellular carcinoma) cell lines (PLC/PRF/5, MAH, BEL-7402, QGY-7703, SMMC-7721) was completed. The scope of observation included cell morphology, growth, frozen, preservation in liquid nitrogen and recovery, chromosome counts, and detection of special material (e.g. AFP, HBsAg). The results of our observation confirmed that the cells are of epithelial origin malignant in nature and are able to produce and secrete AFP. Therefore, they are useful materials for further research on human hepatoma. The PLC/PRF/5 cells preserve the capabilities of producing and secreting HBsAg which existed at the time of establishment of the cell line, and remain to be a special experimental model for the research work on the relationship between hepatitis B virus and hepatocellular carcinoma. The success of introducing cultured cells to consequent transplantation in the nude mice and re-establishing the culture from four different kinds of cell lines provides very useful models for cancer research in the future.

对五株人体肝癌细胞(PLC/PRF/5、MAH、BEL-7402、QGY-7703、SMMC-7721)作了多方面的生物学特性观察,包括形态、生长、冻存与复苏后钓变化、染色体计数、特异物质测定等,证明其为上皮来源,并有恶性特征,能产生与分泌甲胎蛋白,为肝癌研究提供了有用的资料。其中PLC/PRF/5细胞尚保留其建株时原有的产生与分泌乙型肝炎表面抗原(HBsAg)的特性,可继续作为研究乙型肝炎病毒与肝细胞癌关系的一个特殊实验模型。另外四株细胞的裸鼠接种致瘤、连续移植及重建培养的成功,为今后肿瘤研究开创了有利的条件。

 
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