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   fluorescent quantitative polymerase chain reaction 在 临床医学 分类中 的翻译结果: 查询用时:0.857秒
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fluorescent quantitative polymerase chain reaction    
相关语句
  荧光定量pcr
    Detection of Mycoplasma genitalium infection by fluorescent quantitative polymerase chain reaction in pelvic inflammatory disease
    荧光定量PCR检测盆腔炎性疾病生殖道支原体感染的研究
短句来源
    Detection of the ipaH Gene of Shigella Using Molecular Beacon Probe-based Real-time Fluorescent Quantitative Polymerase Chain Reaction
    分子信标荧光定量PCR技术检测志贺菌ipaH基因的研究
短句来源
    Methods A plasmid pHaMDR1/A with mdr1 cDNA was used as a template. Primer Express 2.0 was used for designing of primers and Taq Man-MGB probe for the real-time fluorescent quantitative polymerase chain reaction(FQ-PCR).
    方法以含有目的基因mdr1cDNA的质粒pHaMDR1/A为阳性模板,用Primerexpress2.0引物设计软件设计引物和TaqMan-MGB探针,建立荧光定量PCR检测方法。
短句来源
    Objective : At present, the Taqman fluorescent quantitative polymerase chain reaction is the most wide used clinical gene diagnosis quantitative assay for mycobacterium tuberculosis but it is still an important issue that how we could increase positive rate, reduce the cost and make the reaction time short.
    目的:Taqman探针荧光定量PCR是目前检测结核分枝杆菌应用广泛的临床基因诊断定量方法,虽然该技术已经比较成熟,但是如何提高阳性率,降低成本,缩短反应时间,仍是亟待解决的一个重要问题。
短句来源
    Objective: To detect the ipaH gene of Shigella using molecular beacon probe-based real-time fluorescent quantitative polymerase chain reaction (PCR).
    目的:用分子信标荧光定量PCR技术检测志贺菌ipaH基因。
短句来源
更多       
  荧光定量聚合酶链反应
    Application research on the real-time fluorescent quantitative polymerase chain reaction in human papillomavirus detection
    实时荧光定量聚合酶链反应技术在人乳头状瘤病毒检测中的应用研究
短句来源
    Objective:To evaluate the clinical efficacy of real-time fluorescent quantitative polymerase chain reaction(FQ-PCR)on female low genital human papillomavirus(HPV)detection.
    目的:评价实时荧光定量聚合酶链反应(FQ-PCR)技术在女性生殖道人乳头状瘤病毒(HPV)检测中的临床效用。
短句来源
    A novel fluorescent quantitative polymerase chain reaction method of Mycobacterium tuberculosis DNA using duplex scorpion probe
    新型二聚体蝎型探针技术在结核分枝杆菌荧光定量聚合酶链反应中的应用
短句来源
    Objective To investigate a method and it’s clinical significant of fluorescent quantitative polymerase chain reaction (FQ-PCR) assay to detect Mycoplasma genitalium (MG) infection in patients with pelvic inflammatory disease (PID).
    目的探讨荧光定量聚合酶链反应(fluorescentquantitativepolymerasechainreaction,FQ-PCR)检测盆腔炎性疾病(PID)生殖道支原体(Mycoplasmagenitalium,MG)的临床意义。
短句来源
    Methods A total of 586 cases of postpartum women with Hepatitis B were selected to determine HBV-DNA level in their milk with fluorescent quantitative polymerase chain reaction FQ-PCR.
    方法选择经血清学检测诊断为乙肝携带者的产妇586例(HBV-DNA定量分析呈阳性,免疫学指标提示为大三阳或小三阳),采用荧光定量聚合酶链反应(FQ-PCR)技术测定其乳汁中HBV-DNA含量,并对所有检测指标进行相关性分析。
短句来源
  荧光定量聚合酶链反应
    Application research on the real-time fluorescent quantitative polymerase chain reaction in human papillomavirus detection
    实时荧光定量聚合酶链反应技术在人乳头状瘤病毒检测中的应用研究
短句来源
    Objective:To evaluate the clinical efficacy of real-time fluorescent quantitative polymerase chain reaction(FQ-PCR)on female low genital human papillomavirus(HPV)detection.
    目的:评价实时荧光定量聚合酶链反应(FQ-PCR)技术在女性生殖道人乳头状瘤病毒(HPV)检测中的临床效用。
短句来源
    A novel fluorescent quantitative polymerase chain reaction method of Mycobacterium tuberculosis DNA using duplex scorpion probe
    新型二聚体蝎型探针技术在结核分枝杆菌荧光定量聚合酶链反应中的应用
短句来源
    Objective To investigate a method and it’s clinical significant of fluorescent quantitative polymerase chain reaction (FQ-PCR) assay to detect Mycoplasma genitalium (MG) infection in patients with pelvic inflammatory disease (PID).
    目的探讨荧光定量聚合酶链反应(fluorescentquantitativepolymerasechainreaction,FQ-PCR)检测盆腔炎性疾病(PID)生殖道支原体(Mycoplasmagenitalium,MG)的临床意义。
短句来源
    Methods A total of 586 cases of postpartum women with Hepatitis B were selected to determine HBV-DNA level in their milk with fluorescent quantitative polymerase chain reaction FQ-PCR.
    方法选择经血清学检测诊断为乙肝携带者的产妇586例(HBV-DNA定量分析呈阳性,免疫学指标提示为大三阳或小三阳),采用荧光定量聚合酶链反应(FQ-PCR)技术测定其乳汁中HBV-DNA含量,并对所有检测指标进行相关性分析。
短句来源
  荧光定量聚合酶链反应
    Application research on the real-time fluorescent quantitative polymerase chain reaction in human papillomavirus detection
    实时荧光定量聚合酶链反应技术在人乳头状瘤病毒检测中的应用研究
短句来源
    Objective:To evaluate the clinical efficacy of real-time fluorescent quantitative polymerase chain reaction(FQ-PCR)on female low genital human papillomavirus(HPV)detection.
    目的:评价实时荧光定量聚合酶链反应(FQ-PCR)技术在女性生殖道人乳头状瘤病毒(HPV)检测中的临床效用。
短句来源
    A novel fluorescent quantitative polymerase chain reaction method of Mycobacterium tuberculosis DNA using duplex scorpion probe
    新型二聚体蝎型探针技术在结核分枝杆菌荧光定量聚合酶链反应中的应用
短句来源
    Objective To investigate a method and it’s clinical significant of fluorescent quantitative polymerase chain reaction (FQ-PCR) assay to detect Mycoplasma genitalium (MG) infection in patients with pelvic inflammatory disease (PID).
    目的探讨荧光定量聚合酶链反应(fluorescentquantitativepolymerasechainreaction,FQ-PCR)检测盆腔炎性疾病(PID)生殖道支原体(Mycoplasmagenitalium,MG)的临床意义。
短句来源
    Methods A total of 586 cases of postpartum women with Hepatitis B were selected to determine HBV-DNA level in their milk with fluorescent quantitative polymerase chain reaction FQ-PCR.
    方法选择经血清学检测诊断为乙肝携带者的产妇586例(HBV-DNA定量分析呈阳性,免疫学指标提示为大三阳或小三阳),采用荧光定量聚合酶链反应(FQ-PCR)技术测定其乳汁中HBV-DNA含量,并对所有检测指标进行相关性分析。
短句来源

 

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Objective: To detect hepatitis B virus (HBV) in semen and to find if sperm washing removes HBV DNA. Methods: Amplification by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect viral DNA in semen samples of 57 HBsAg-seropositive patients.Twenty-one semen samples were washed,and spermatozoa were separated by discontinuous Percoll gradient centrifugation and the `swim-up' method.The HBV DNA in post-washing samples was measured by FQ-PCR. Results: HBV DNA was detected...

Objective: To detect hepatitis B virus (HBV) in semen and to find if sperm washing removes HBV DNA. Methods: Amplification by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect viral DNA in semen samples of 57 HBsAg-seropositive patients.Twenty-one semen samples were washed,and spermatozoa were separated by discontinuous Percoll gradient centrifugation and the `swim-up' method.The HBV DNA in post-washing samples was measured by FQ-PCR. Results: HBV DNA was detected in 15 of 62 semen samples,the positive rate was 24.19%.There were 7 positive cases in 21 samples before washing,the 6 cases except one were positive after washing. Conclusion: Part of semen of HBV infected patients is infective,and sperm washing does not remove HBV.It is cautious to use semen of the HBV infected patients in IVI or IVF.

目的 :检查HBV感染者精液是否存在乙型肝炎病毒 (HBV) ,探讨精子洗涤是否能去除其中的HBVDNA。方法 :用实时荧光定量PCR(FQ -PCR)法检测 5 7名血清HBsAg阳性患者精液中HBVDNA ,采用Percoll非连续梯度离心及上游法分离其中 2 1份精液 ,并对洗涤后标本进行HBVDNA检测。结果 :6 2份精液标本中有 15份HBVDNA阳性 ,阳性率为2 4 .19% ;2 1份用于洗涤的精液标本中 ,有 7份HBVDNA阳性 ,洗涤后仍有 6份标本阳性。结论 :部分HBV感染者精液具有传染性 ,精子洗涤不能完全去除精液中的乙肝病毒。将HBV感染者的精液用于人工授精或体外受精应慎重。

Objective To investigate excretion of severe acute respiratory syndrome coronavirus RNA (SARS CoV) in stool of SARS patients Methods SARS CoV RNA was detected in stool specimens with fluorescent quantitative polymerase chain reactions (FQ PCR) in 101 SARS patients on the 10 to 55 days after onset, 27 non SARS patients and 400 individuals with health check up Results SARS CoV RNA was positive in stool specimens by FQ PCR in 58 of 101 SARS patients (57 4%), and all negative...

Objective To investigate excretion of severe acute respiratory syndrome coronavirus RNA (SARS CoV) in stool of SARS patients Methods SARS CoV RNA was detected in stool specimens with fluorescent quantitative polymerase chain reactions (FQ PCR) in 101 SARS patients on the 10 to 55 days after onset, 27 non SARS patients and 400 individuals with health check up Results SARS CoV RNA was positive in stool specimens by FQ PCR in 58 of 101 SARS patients (57 4%), and all negative in 27 non SARS patients and 400 healthy individuals Positive rate of SARS CoV RNA was 100%(8/8), 67 7%(21/31), 47 4%(27/57) and 40.0% (2/5) on the 10~19, 20~29, 30~39 and 40~55 days after onset of fever, respectively, with values of logarithm of SARS CoV RNA load of 6 06 ±2 05, 4 51 ±1 23, 3 82 ±1 44 and 3 57 ±1 25, respectively Conclusion Positive rate and load of SARS CoV RNA in stool of SARS patients was the highest at their acute phase, and decreased with the extension of its course

目的 研究严重急性呼吸综合征 (SARS)患者粪便中排出SARS冠状病毒 (SARS CoV)RNA的情况。方法 采用荧光定量聚合酶链反应法 (FQ PCR)检测 10 1例病程为 10~ 5 5d的SARS患者、2 7例非SARS患者和 4 0 0名健康体检人群粪便中SARS CoVRNA。结果  10 1例SARS患者的粪便标本中 5 8例 (5 7 4 % )SARS CoVRNA阳性 ;2 7例非SARS患者和 4 0 0名健康体检人群的粪便标本均为阴性。SARS患者发病后 10~ 19、2 0~ 2 9、30~ 39和 4 0~ 5 5d ,粪便中SARS CoVRNA阳性率分别为 10 0 % (8/ 8)、6 7 7% (2 1/ 31)、4 7 4 % (2 7/ 5 7)和 4 0 0 % (2 / 5 )。粪便中SARS CoVRNA载量对数值分别为 6 0 6± 2 0 5、4 5 1± 1 2 3、3 82± 1 4 4和 3 5 7± 1 2 5。结论 SARS患者急性期粪便中SARS CoVRNA阳性率和载量对数值最高 ,且随病程延长而下降

To develop and compare several methods for detection of SARS-associated with Coronavirus, the specimens from identified patients with SARS, suspect patients and autopsy were collected respectively. Methods including cell culture with three different cell, fluorescent quantitative polymerase chain reaction, and indirect immunofluorescent assay were applied. The results showed that in 57 specimens detected by cell culture method, 5 from throat-swab and throat-washing showed positive, and 7 specimens from...

To develop and compare several methods for detection of SARS-associated with Coronavirus, the specimens from identified patients with SARS, suspect patients and autopsy were collected respectively. Methods including cell culture with three different cell, fluorescent quantitative polymerase chain reaction, and indirect immunofluorescent assay were applied. The results showed that in 57 specimens detected by cell culture method, 5 from throat-swab and throat-washing showed positive, and 7 specimens from autopsy showed positive. In 130 specimens detected by fluorescent quantitative polymearase chain reaction method, 38 in 57 specimens from cases of identified SARS patients, 7 in 14 specimens from the case of closely contact, and 12 in 59 specimens from the cases of suspect SARS patients showed positive. It concludes that the development of rapid, accurate and efficient methods is important for epidemical investigation and clinical diagnosis of SARS.

目的 在实验室建立SARS病原学检测方法。方法 采集临床诊断为SARS患者、疑似病人漱口液、用细胞培养分离,荧光定量PCR方法检测。病原体用间接免疫荧光鉴定。结果 漱口液、咽拭子液样本57份作病原体分离,阳性数5份,阳性率8.8%尸解标本7份,其中肺部组织3份、气管黏液、淋巴液、肺积液、心包液各1份。结果细胞分离到7株病原体。阳性率100%。荧光定量PCR方法检测临床确诊SARS患者标本57份,检出阳性结果38份,阳性率为66.7%。密切接触者标本14份,检出阳性结果7份,阳性率为50.0%。疑似患者标本59份中检出阳性12份,阳性率为20.3%。两种方法的比较,统计学上有显著性差异(P<0.001)。结论用荧光定量PCR方法检测SARS患者病原体敏感性高,可靠性和重复性好,优于细胞病毒分离的方法,可以作为SARS临床早期诊断和流行病学调查的重要指标之一。

 
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