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two copies
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  双拷贝
     Results Two copies of APC were shown in 48% interphase nuclei and 3 copies in 22%;
     结果 APC 基因双拷贝的核为48 % ,三拷贝的核占22 % ;
短句来源
     The Immune Responses of the Fusion Protein Consisted of Two Copies of T-cell and B-cell Epitopes of Food and-mouth Disease Virus VP1 Type O and LTB and STⅠ Enterotoxins of Escherichia coli
     O型口蹄疫病毒VP1 T细胞、B细胞表位双拷贝基因与大肠杆菌LTB、STⅠ肠毒素基因融合表达产物的免疫应答
短句来源
     A new recombinant plasmid named pSV2 DHBR1-32 containing an Xho I -Bgl II fragment of S gene of hepatitis B virus was constructed by deletion of a 2.8 kb fragment of HBV DNA from the pSV2DHBR2-32 which is a pSV2 based plasmid containing two copies of the adr subtype of HB-sAg gene, previously reported by our laboratory.
     使用本研究室以前构造的双拷贝乙肝病毒重组DNA质粒pSV_2DHBR2-32经改造构成pSV_2DHBR1-32。
短句来源
     The transcription profile between two copies of the duplicates is quite different, which demonstrates the existence of the functional difference between them, just like the human trs20 duplicates—SEDL and MIP-2A.
     其中双拷贝的4对基因,其两个拷贝均位于不同的染色体上,且在同种组织中的转录强度往往有很大的差异,因此不排除它们还具有其它功能的可能性,正如人的trs20的两个拷贝SEDL基因和MIP-2A基因那样。
短句来源
     Since in our experiments, cotransformation was successful with a single copied HBV-DNA, it seems to indicate that two copies of HBV-DNA in tandem are not essential.
     单拷贝HBV-DNA转化成功,似说明首尾相连的双拷贝HBV-DNA并非必需。
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  “two copies”译为未确定词的双语例句
     In eukaryotic cells, the fundamental unit of chromatin is nucleosome, which is composed of a histone octamer with two copies of H2A, H2B, H3 and H4 and 146 base pairs of DNA.
     真核细胞中,核小体是染色质的基本单位,它由两个拷贝H2A、H2B、H3和H4所组成的核心组蛋白八聚体,及缠绕于其上的146bp DNA构成。
短句来源
     Thurmulus has two copies of tatC genes and one copy of tatA gene.
     嗜热杆菌具有2个拷贝的tatC基因和一个拷贝的tatA基因,且其中一个tatC基因和tatA基因紧密相连。
短句来源
     The interaction of genetic polymorphisms of CYP2E1 and GSTM1 on susceptibility to chronic benzene poisoning was analyzed and our finding suggested that the individuals carrying both one or two copies of CYP2E1 96bp insertion and GSTM1 null genotype could be more susceptible to benzene toxicity( OR=3 75,95%CT:0 86~17 27,P <0 05).
     将CYP2E1和GSTM1进行联合作用分析 ,结果提示在CYP2E1基因 96bp插入阳性型的个体中 ,同时携带GSTM1缺失型的个体更易发生慢性苯中毒 (OR =3 75 ,95 %CI :0 86~ 17 2 7,P <0 0 5 )
短句来源
     Gene immunization experiments in dogs preimmunized with an inactivated vaccine showed that the pcDNAK-VP2C2 vector containing two copies of canine-specific CpG induced higher secondary immune response than that of the pcDNA3-VP2C1 vector.
     对于经CPV灭活苗初次免疫的毕格犬,用pcDNAK-VP2C2(含2个拷贝CpG基序)质粒免疫产生的再次免疫应答优于pcDNA3-VP2C1。
短句来源
     Southern blot analysis showed that there were two copies of the gene in the three accessions.
     Southern blot分析结果表明,三种材料中该基因均含有两个拷贝.
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  相似匹配句对
     Two.
     二、末日意识
短句来源
     Two.
     二.
短句来源
     One Story, Two Different Copies
     一种故事 两种说法
短句来源
     Southern blot analysis indicated that the gene has two copies in the genome of cotton.
     Southern杂交结果表明 :该基因在棉花基因组中有两个拷贝。
短句来源
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  two copies
B) (i) IfG1=G2=L is a polycyclic group andG=G1*HG2 is the amalgamated product of two copies of the groupL by a normal subgroupH, thenG is finitely approximable with respect to conjugacy.
      
Bacteria were isolated from the nasopharynx of BALB/c mice and electroporated with pUR290(NS1)2 containing two copies of tick-borne encephalitis virus (TBEV, strain Sofjin) NS1 under the control of the lac promoter.
      
Type IIE REs have provided an example of a new type of DNA-protein recognition: two copies of one recognition sequence interact specifically with two different amino acid sequences and two different structural motifs of one polypeptide chain.
      
Two copies of the 16S rRNA gene, rrnAand rrnB, of the type strain 17Tof the thermophilic sulfate-reducing bacterium Desulfotomaculum kuznetsoviiwere cloned and completely sequenced.
      
melanogaster isolated from the Uman' natural population can be, according to our data, explained by repeated inversions and reinversions of the gene regulatory region located between the two copies of the hobo transposon.
      
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There are two different ways for construction of ways for walsh functions ,i.e.shift and symmetry copies.In this paper,an interrelationship between these two copies is derived and an illustrative example is given So these two copies can be united.

沃尔什(Walsh)函数有平移复制和对称复制这两种不用的生成方式,本文导出了这两种生成方式的相互转换关系,从而可将其完全统一起来。文中并给出例子加以说明。

Two plasmids containing a single copy of hepatitis B virus (HBV) genome (ayw or adw) have been introduced into mouse Ltk-cells by calcium precepitation and cotransformation with cloned herpes simplex virus thymidine kinase (tk) gene. Under the selective pressure of HAT medium, 6 cloned tk~+ cell lines were found to excrete specific HBsAg in cell culture medium. Two cell lines, pA-3 and pC-3 were selected for further studies. These cell lines were found to be composed of HBsAg positive and HBsAg negative cells...

Two plasmids containing a single copy of hepatitis B virus (HBV) genome (ayw or adw) have been introduced into mouse Ltk-cells by calcium precepitation and cotransformation with cloned herpes simplex virus thymidine kinase (tk) gene. Under the selective pressure of HAT medium, 6 cloned tk~+ cell lines were found to excrete specific HBsAg in cell culture medium. Two cell lines, pA-3 and pC-3 were selected for further studies. These cell lines were found to be composed of HBsAg positive and HBsAg negative cells on subcloning. The pC-3-1 subline cells obtained from pC-3 cell line by subcloning retained their ability to excrete HBsAg after 16o days passage. This phenomenon shows. that the subcloning procedure is essential for obtaining stable cell lines continously excreting HBsAg. The HBsAg excreted into the cell culture medium was shown by immunoelectromicroscopy to be composed of particles about 22nm diameters, being similar to those found in hu- man serum. Since in our experiments, cotransformation was successful with a single copied HBV-DNA, it seems to indicate that two copies of HBV-DNA in tandem are not essential.

用共转化技术分别将两种亚型的单拷贝HBV-DNA重组质粒与HSV-tk基因一起导入Ltk细胞获得成功。6系克隆化的tk~+细胞分泌HBsAg。分泌量较高的pA-3及PC-3两系细胞分泌物为特异性HBsAg,并含有约22nm大小的颗粒性HBsAg。初次细胞克隆HBsAg表达不稳定,再次克隆获得PC-3-1亚系传代160天仍表达HBsAg,说明再次克隆是选择HBsAg表达较稳定的细胞系的必要手段。单拷贝HBV-DNA转化成功,似说明首尾相连的双拷贝HBV-DNA并非必需。

Using a simple, quick and efficient method, namely, partially digesting of pLJ3 DNA with EcoRI, converting protruding termini of DNA to blunt ends, joining blunt cnds; cutting with EcoRI again, and joining together DNA moleculet with compatible cohesive termini, we eliminated a EcoRI site upstream from lacuv5 promotor of pLJ3 and got a new plasmid pFG1. pFG1 contains two copies of lacuv 5 promoter (including Lacuv5 promotor, operator gene and the SD sequence of LacZ gene). In pFG1 there is a EcoRI site...

Using a simple, quick and efficient method, namely, partially digesting of pLJ3 DNA with EcoRI, converting protruding termini of DNA to blunt ends, joining blunt cnds; cutting with EcoRI again, and joining together DNA moleculet with compatible cohesive termini, we eliminated a EcoRI site upstream from lacuv5 promotor of pLJ3 and got a new plasmid pFG1. pFG1 contains two copies of lacuv 5 promoter (including Lacuv5 promotor, operator gene and the SD sequence of LacZ gene). In pFG1 there is a EcoRI site at the downstream of Lacuv 5 promotor and just two bases before the point of translation of the lacz gene, so that we could insert exogenous gene at the EcoRI site conveniently. The PFGI provides efficient promotors and SD sequence for exogenous gene expression in E. coli.

用EcoRI部分酶切质粒pLJ3,将其粘末端变成平末端,进行平末端连接;再用EcoRI完全酶切,进行粘末端连接,最后我们消去了pLJ3质粒Lacuv5启动子上游的EcoRI酶切位点,得到了一种新质粒pFGI。pFGI含有两个串联的Lacuv5启动子片段,其中包括Lacuv5启动子,操纵基因及LacZ基因的SD序列和一个四环素抗性基因,紧挨LacZ基因的SD序列后,有一EcoRI酶切位点,可方便地插入外源基因,为外源基因在大肠杆菌中的表达提供有效的启动子和核糖体结合所需的SD序列。

 
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