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toxin protein
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  毒素蛋白
     Comparative study of two purification methods of Bacillus thuringiensis HD-73 toxin protein
     两种纯化苏云金杆菌HD-73毒素蛋白方法的比较研究
短句来源
     And,as the development of detecting techniques for Bt toxin protein and soil were made by oversea researchers.
     加之Bt毒素蛋白检测技术的发展 ,国外学者们围绕纯化毒素蛋白与土壤的相互关系等方面开展了大量研究 ,并取得了一些结果 .
短句来源
     Methods such as amplified ELISA, amplified BA ELISA and amplified Dig ELISA using several kinds of amplified immunoreagents prepared by ourselves were established. When these methods were used for measurement of SEA, SEB, SEC and TSST of 4 kinds of staphylococcal enterotoxins, the sensitivity could reach the level of 63 pg/ml of toxin protein.
     使用自制的几种放大免疫试剂,先后建立了放大ELISA、放大BA-ELISA及放大Dig-ELISA等方法,在应用于四种葡萄球菌肠毒素(SE)的SEA、SEB、SEC及TSST的测定中,灵敏度均能达到63pg/ml的毒素蛋白水平
短句来源
     In this study, a DNA fragment encoding the Domain Ⅱ andⅢ of Cry1Aa toxin was fused with Cry3A toxin protein. An expression plasmid with the fusion protein was also created for further research relating to genetic engineered Bt and transgenic crops.
     利用基因重组技术对鳞翅目昆虫具专一活性的毒素蛋白Cry1Aa与专一性有关的结构域编码区与对鞘翅目昆虫具专一活性的毒素蛋白Cry3A编码基因融合,构建成融合表达载体,为进一步构建Bt工程菌和Bt转基因植物奠定基础。
短句来源
     8. The primary result of insect bioassay on eight transformants of Nan 160 and two transformants of Xue60 showed that toxin protein was produced from some of them and the expression levels of Bt gene were different.
     8、对8株Nan160和2株Xue60花椰菜T_0代转基因植株进行了初步室内抗小菜蛾幼虫鉴定。 根据结果我们可以推断,毒素蛋白在转基因植株中已经表达,但表达量在不同的植株里存在着一定的差异。
短句来源
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  杀虫蛋白
     Degradation of Cry1Ab Toxin Protein Expressed by Bt Transgenic Rice in Paddy Soils
     转Bt基因水稻Cry1Ab杀虫蛋白在水稻土中的降解
短句来源
     The antigen and antiserum of crystal toxin protein of Bacillus thuringiensis srtains HD-1 and HD-68 which were soluble in alkali were prepared. A Dot-ELISA for Bt toxin protein had been developed. The detectable level of Bt toxin protein by Dot-ELISA was 87. 5ng/ml.
     用苏云金芽孢杆菌HD-1和HD-68晶体杀虫蛋白的碱溶产物作为抗原,制备相应的 抗血清,建立了检测 Bt杀虫蛋白的 Dot-ELISA,可检测 Bt杀虫蛋白的最低水平为 87.5ng/ml。
短句来源
     Detection of Bt Toxin Protein in Transgenic Cotton by Dot-ELISA
     利用Dot- ELIS A检测Bt棉杀虫蛋白的研究
短句来源
     The inheritance of Bt gene in Bt transgenic cotton has b een studied b y determining the Bt crystal toxin protein of the populations, strains and lines from F 1 to F 5 and BC 1,BC 2 generations derived from the crosses of tran sgenic cotton line R55 with a lot of normal parents. ELISA(Enzyme-linked Immuno sorbent Assay) method was used for the determination of Bt crystal protein and c ompared with the biological identification in the field.
     以转 Bt基因抗虫棉 R55为材料 ,利用ELISA( Enzyme-linked Immunosorbent Assay)检测方法 ,通过对不同亲本与抗虫亲本 R55杂交F1~ F5、BC1、BC2 世代材料 Bt晶体杀虫蛋白的定性、定量测定 ,结合大田自然感虫条件下的抗棉铃虫鉴定 ,研究了转 Bt基因抗虫棉 Bt基因的遗传规律。
短句来源
     Comparison among Three ELISA Methods for Bt Toxin Protein
     3种ELISA法检测Bt杀虫蛋白的比较研究
短句来源
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  毒蛋白
     Expression and Root Exudation of Cry1Ab Toxin Protein in cry1Ab Transgenic Rice and Its Residue in Rhizosphere Soil
     转cry1 Ab基因水稻中毒蛋白的表达、分泌及其在土壤中的残留
短句来源
     The proteins from endosperm, root, stem, leaf, leafstalk of Jatropha curcas L. and their calli were hybridized the seed toxin protein (curcin) of Jatropha curcas L. by Western blot.
     然后我们以麻疯树(Jatropha curcas L)的胚乳、根、茎、叶、叶柄及其诱导出的愈伤组织为材料,对麻疯树种子毒蛋白(curcin)作Western-blot杂交。
短句来源
     Ribosome-inactivating protein (RIP) is a sort of toxin protein existing widely in higher plant which can inhibit the synthesis of protein.
     核糖体失活蛋白(ribosome-inactivating protein,RIP)是一种广泛存在于植物中的可抑制蛋白质生物合成的毒蛋白
短句来源
     The results showed that content of Cry1Ab toxin protein in the shoot and root of KMD were 3 23~8 22 and 0 68~0 89μg/g from early tillering to maturing stage, respectively.
     结果表明 ,分蘖始期至成熟期 ,克螟稻地上部和根部中的Cry1Ab毒蛋白的表达量 (FW)分别为 3 2 3~ 8 2 2 μg/ g和 0 6 8~ 0 89μg/g .
短句来源
     The expression and root exudation of Cry1Ab toxin protein in cry1Ab transgenic rice (KMD) and its residue in rhizosphere soil were investigated.
     研究了转cry1Ab基因水稻 (克螟稻 )中Cry1Ab毒蛋白的表达、根系分泌及其在土壤中的残留规律 .
短句来源
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  “toxin protein”译为未确定词的双语例句
     The serrtype H5a5b was classifledfrom the surburb, Yangzhou in 1982. It is similar to the strains 1593, 2362 recomended by WHO in the characteric of physiology and biochemistry But its toxicity is 2.78—4.2 times than the strain 1593 2.78 times more than the strain 2362. The molecular weight of toxin protein of BS 10 is 45KD), the proteinmainly exists in the sporange andspore cell of BS-10 with a square parasporal cristaline.
     1982年从江苏扬州市郊区孳生地分离到,经鉴定属血清5_a5b型,其生理生化特性与世界卫生组织(WHO)推荐的1593、2362菌株相似,但其毒力比1593菌株高1.78—3.2倍,比2362的发酵毒力提高1.79倍。
短句来源
     In tobacco capsula, the average Bt toxin protein expression level of A line and B line was 1.5 times and 2.4 times than CK respectively. In tobacco capsular hull, the toxin expression level of A and B was 1.5 times and 1.9 times compared with CK respectively;
     在烟草的蒴果中,A系的表达量是CK系的1.5倍,B系的表达量是CK系的2.4倍:在烟草的蒴果壳中,A系的表达量是CK系的1.5倍,B系的表达量是CK系的1.9倍;
短句来源
     This 33-residue toxin with molecular weight of 3853.69 Da shows 60% and 48.5% sequence identity with lectin-like peptide SHL-I previously isolated from the venom of the same spider and the Toxin Protein 5 purified from Mexican red knee tarantula (Brachypelma smithii), respectively.
     该毒素含有六个半胱氨,与同种蜘蛛毒液中纯化的凝集活性肽SHL-Ⅰ及墨西哥红膝Tarantula蜘蛛(Brachypelma smithii)毒液中纯化的TxP5具有较高的同源性,分别为60%和48.5%。
短句来源
     Improved histochemical staining for GUS activity,PCR and Western blotting were used to detect the population of Bt rice crossed to conventional rice varieties A total of 392 plants expressing Bt toxin protein were found in 394 GUS positive plants.
     利用PCR、GUS染色和Western印迹杂交技术检测了Bt水稻杂交后代群体,发现在394株GUS阳性株中,共有392株表达Bt蛋白,协同表达株率达99.49%。
短句来源
     The reactivity of the 12 recombinant fusion proteins of cholera toxin protein and 4 HCV epitopes to human and- HCV positive sera was evaluated in this work.
     系统研究了通过霍乱毒素B亚基与HCV的4个抗原决定簇进行基因融合所表达的12种融合蛋白中HCV抗原决定簇的反应原性,探索了以融合蛋白为抗原,进行抗-HCV检测的途径。
短句来源
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  toxin protein
It was suggested that production of Cry4Ba protein and binary toxin protein interacted synergistically, thereby increasing their mosquito-larvicidal toxicity.
      
sphaericus than the transformed strains expressing Cry4Ba protein or binary toxin protein independently.
      
Transformed strain Bt-BW611, expressing both Cry4Ba protein and binary toxin protein, was more than 40-fold more toxic to Culex pipiens larvae resistant to B.
      
The 3.7 kb XbaI fragment harbouring the cryIVB gene which encoded a 130 kDa mosquitocidal toxin protein from Bacillus thuringiensis subsp.
      
Double-antibody sandwich ELISA analysis revealed Bt toxin protein expression in the transgenic plants.
      
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Enzyme-Linked immunosorbent assay was used to detect and quantitate the insecticidal crystal toxin proteins of B. thuringiensis HD-1 and commercial preparations. The minimal detectable value by indirect method of ELISA was 7.79 ng/0.15ml. The total crystal protein in standard sample HD-1-S-1980 and a commercial Bt emulsion (produced by Hubei Academy of Agricultural Sciences) was measured to be 108.06 mg per gram of dry powder and 4.57-6.17 mg per ml of emulsion, respectively. With these two values,...

Enzyme-Linked immunosorbent assay was used to detect and quantitate the insecticidal crystal toxin proteins of B. thuringiensis HD-1 and commercial preparations. The minimal detectable value by indirect method of ELISA was 7.79 ng/0.15ml. The total crystal protein in standard sample HD-1-S-1980 and a commercial Bt emulsion (produced by Hubei Academy of Agricultural Sciences) was measured to be 108.06 mg per gram of dry powder and 4.57-6.17 mg per ml of emulsion, respectively. With these two values, the relative toxicity potency of a commercial emulsion can then be calculated.

用酶联免疫吸附测定(ELISA)检测Bt伴孢晶体毒素蛋白质,最低可检值7.79ng/0.15ml,灵敏、精确,具高度专化性。测定标准品HI—1—S—1980和Bt乳剂产品中伴孢晶体蛋白质含量,分别为108.06mg/g粉剂和4.57—6.17mg/ml乳剂。用标准品与待测样品(Bt乳剂)的晶体蛋白质含量,就可计算出待测样品的相对毒力效价。ELISA与昆虫生测之间有良好的相关性。

The delta-endotoxin protein of Bacillus thuringiensis subsp. kurstaki HD-1 was isola-ted from parasporal crystals. The purified toxin protein had a molecular weight of 68000showed on SDS-PAGE gel. Monoclonal antibody against delta-endotoxin protein was ra-ised by the hybridoma technique and detected by an indirect enzyme. linked immunosorb-ent assay (ELISA).High title of the monoclonal antibodylin supernatant of cell culture hadbeen detected after neatly half year culture in vitro. The high...

The delta-endotoxin protein of Bacillus thuringiensis subsp. kurstaki HD-1 was isola-ted from parasporal crystals. The purified toxin protein had a molecular weight of 68000showed on SDS-PAGE gel. Monoclonal antibody against delta-endotoxin protein was ra-ised by the hybridoma technique and detected by an indirect enzyme. linked immunosorb-ent assay (ELISA).High title of the monoclonal antibodylin supernatant of cell culture hadbeen detected after neatly half year culture in vitro. The high specificity of the antibodycould be used for detection of delta-endotoxin gene expression in genetic engineeredproducts.

从苏云金芽孢杆菌HD-1伴胞晶体中分离得到的毒素蛋白经过Sepharose 6B和DEAE-Sephadex DE-52得到了纯化,纯化后的蛋白在SDS-PAGE上呈现一条带,其分子量约为68 000。用纯化蛋白作为抗原免疫小鼠,通过杂交瘤技术建立了抗HD-1 δ-内毒素蛋白的单克隆抗体杂交瘤细胞株。经过半年的体外培养,大多数细胞株仍能持续分泌高滴度的单克隆抗体。文中还讨论了抗体的特异性及理化特性。此单克隆抗体细胞株的建立将为植物基因工程产物的检测提供有力工具。

Bacillus Sphaericus-10 (BS-10) is a pathogenic bacterium on the larvae of mosquito.It can kill the Culex pipiens pallens Coquillett highly. The serrtype H5a5b was classifledfrom the surburb, Yangzhou in 1982. It is similar to the strains 1593, 2362 recomended by WHO in the characteric of physiology and biochemistry But its toxicity is 2.78—4.2 times than the strain 1593 2.78 times more than the strain 2362. The molecular weight of toxin protein of BS 10 is 45KD), the proteinmainly exists in the sporange...

Bacillus Sphaericus-10 (BS-10) is a pathogenic bacterium on the larvae of mosquito.It can kill the Culex pipiens pallens Coquillett highly. The serrtype H5a5b was classifledfrom the surburb, Yangzhou in 1982. It is similar to the strains 1593, 2362 recomended by WHO in the characteric of physiology and biochemistry But its toxicity is 2.78—4.2 times than the strain 1593 2.78 times more than the strain 2362. The molecular weight of toxin protein of BS 10 is 45KD), the proteinmainly exists in the sporange andspore cell of BS-10 with a square parasporal cristaline. When the larve of mosquito ate some spores, at most, 6 hr. later it would die, and the death is right proportion to the quantity of diet, and the younger the more sensltive. Its control spectrumas follows: Culex pipiens Coquillett C. pipiens quinuefascitatus Say C.trilaencorhynchus Giles Anopheles sin ensis Wiedean Ae. aegypti Linnaeus. Usage of BS-10 is 3-5ml/m~2, 80% of the death after 24 hr.; 95-100% after 48 hr., generaly 15-25Days of persistence. What's more, the product of BS-10 is no harm to the human, beast, poultry, and aquaticlife, and no pollution of environment. Key words: Bacillus sphaericus-10 larvae of mosquito BioIogical characteristic Product of BS-10

球形芽孢杆菌BS-10(Bacillus Sphaericus-10)是蚊幼虫的一种病原体,对库蚊等蚊幼虫具有极高的毒杀作用。1982年从江苏扬州市郊区孳生地分离到,经鉴定属血清5_a5b型,其生理生化特性与世界卫生组织(WHO)推荐的1593、2362菌株相似,但其毒力比1593菌株高1.78—3.2倍,比2362的发酵毒力提高1.79倍。BS-10的杀蚊毒素蛋白的分子量为45KD,主要分布于孢子囊、芽孢壁内,并有菱形的伴孢晶体毒素。当蚊幼吞食一定数量的芽孢后即中毒死亡,吞食数与死亡速度成正相关。最快6h可毒杀,但蚊幼的龄期越小越敏感。它的杀蚊谱为:淡色库蚊>致乏库蚊>凶小库蚊>三带喙库蚊>中华按蚊>埃及伊蚊。BS-10灭幼剂使用量为每m~2投药3~5ml,24h蚊幼死亡率为80%左右,48h为95~100%;随着用药量增加,持效期延长,一般为15~25天。BS-10对人畜、禽及水生生物安全无毒,不污染环境。

 
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