ResultsThe optimized nutrient medium composition is 20% corn steep liquor 300 ml/L,beef extract 55 g/L,and glutamic sodium 5 g/L,and initial pH 7.2.The seed culture was inoculated into flasks in a 2% ratio,incubated at 200 r/min,30℃ for 7 h and induced the target protein expression at 42℃ for 6 h.
colli expressing GST-Melittin-88ArgIL-2.MethodsThe range of medium pH,induction temperature,induction time and revulsivum concentration and expression of the recombinant protein were analyzed and optimized with fermentation bottle.
Methods Investigating the effect of parameters on the growth of pichia pastoris and the expression of aim protein, including kinds of culture media, density of seed cells, concentration of glycerol and methanol, pH, flow speed and inducing time of methanol, To detect rhsTRAIL's biological activities of inducing apoptosis by transmission electron microscope.
The 1 M urea modification gave 11S soy protein the highest adhesive strength in all the wood specimens. 7S soy protein modified with 3 M urea had higher adhesive strength with hardwood than US soy protein modified with 3 M urea.