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restriction enzymes analysis
相关语句
  酶切分析
     RESTRICTION ENZYMES ANALYSIS OF IBV GUANGDONG LOGICAL STRAIN D41 S1 GENE PCR PRODUCT AND ITS SIGNIFICANCE
     IBV广东地方株D41S1基因PCR产物的酶切分析及其意义
短句来源
     WT5BZ]According to the data of the human,chicken and rat ESR sequence,a pair of primer is designed. One DNA fragment of the pig ESR gene was amplified successfully by PCR,and was cloned into pGEM T Easy vector. The fragment was further identified by PCR,restriction enzymes analysis and sequence analysis.
     参考人、鸡、鼠的ESR基因序列 ,设计一对引物 ,采用PCR技术扩增出猪ESR基因一段DNA片段 ,将该片段克隆到pGEM TEasy质粒中 ,重组质粒用PCR扩增和酶切分析进行阳性克隆鉴定 ,然后测定核苷酸序列 ,并推导其氨基酸序列。
短句来源
     The recombinant plasmid pcDNA3-5401 was stable within the host strain in vitro and in vivo as shown by restriction enzymes analysis and PCR identification of the 5401 gene.
     结果表明,利用该减毒沙门氏菌作为载体具有相对安全性,用限制性酶切分析和PCR鉴定证实,体内试验和体外培养的重组质粒在受体菌Z J111菌株内比较稳定。
短句来源
  “restriction enzymes analysis”译为未确定词的双语例句
     The protein expression of CC10 was detected by the techniques of immunofluorescence and Western blot. Results After the identification of restriction enzymes analysis and sequencing,the recombinant plasmidpcDNA3.1-hCC10 was confirmed which contained the correct and entire nucleotide sequence of the CC10 DNA in pcDNA3.1. The expression of CC10 in protein level ascend in A549 cells transfected with reconstructive plasmid.
     结果用酶切重组质粒并行序列鉴定,人CC10基因已经正确克隆到真核细胞表达载体pcDNA3.1中,体外染pcDNA3.1-hCC10的肺腺癌A549细胞中CC10蛋白表达明显增高。
短句来源
     The recombinant plasmid pSNAV-hVEGF165 was identified by restriction enzymes analysis and sequencing analysis, and then transfered to the HEK293 cell and VEC by lipofectamine mediated gene transfer method.
     经酶切及测序鉴定后,用脂质体介导pSNAV-hVEGF165转染HEK293细胞和血管内皮细胞。
短句来源
     The IL-2 gene was inserted into the bacterial plasmid PET-28a, resulting in the construction of PET-28a-IL2 prokaryotic expression plasmids. The recombiant plasmid PET-28a-IL2 containing IL-2 gene were identified by restriction enzymes analysis and PCR method and sequenced to confirm its rightness.
     将该目的基因克隆到原核表达载体PET-28a,获得的重组质粒PET-28a-IL2经酶切、PCR及序列测定,表明IL-2基因插入的位点、大小与读码框均正确。
短句来源
     Results:The analysis of sequence were corresponded to the data from Genebank. The pEGFP-C1/B7 was confirmed to be constructed successfully by RT-PCR and restriction enzymes analysis.
     结果经PCR及酶切鉴定,证实成功构建了含B 7-1基因的真核表达重组体pEGFP-C 1/B 7,重组子测序结果与国际基因文库中m B 7-1序列相符。
短句来源
     To express the Cap protein in insect cells,PCV2-ORF2 gene was amplified by PCR and inserted into a baculovirus transfer plasmid vector. The recombinant plasmid was confirmed using restriction enzymes analysis and DNA sequencing.
     为了在昆虫细胞表达Cap蛋白,本研究采用PCR扩增PCV2-ORF2编码基因,将PCR产物插入到昆虫杆状病毒转移载体上,经酶切反应及DNA序列分析得到验证。
短句来源
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  相似匹配句对
     60 Recombinants were digested with 3 restriction enzymes.
     用三种识别六碱基的限制性内切酶两两组合消化重组质粒。
短句来源
     The PCR products were digested with restriction enzymes Mse I .
     PCR产物用限制性内切酶Mse I消化。
短句来源
     Enzymes in winemaking
     酶在葡萄酒中的应用
短句来源
     The recombinant was screened by PCR detection or restriction enzymes analysis.
     经PCR检测和内切酶分析鉴定含MOMP基因的重组子质粒。
短句来源
     Immobilized enzymes
     固相酶
短句来源
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  restriction enzymes analysis
The restriction enzymes analysis of mitDNA shows that no specific fragmentation occurred after acriflavine treatment.
      
___TAGSTART___BR___TAGEND___Canonical discriminant analysis of four morphometric characters of juveniles and restriction enzymes analysis of ribosomal DNA sequences were used to distinguish Heterodera arenaria, H.
      
Restriction enzymes analysis of preliminary clones pLBG-1 (O+) and pLBG-2 (Oc) were carried out.
      


A rapid extraction procedure for plasmid DNA and the effect of experimental conditions on the results were studied. The results showed that the procedure might be simplified. Plasmid DNA extracted in this way is pure enough to be used in transformation experiment and restriction enzyme analysis. The method is simple enough to be used in screening recombinant plasmid DNA and doing molecular genetics experiment.

本文研究在各种实验条件下快速抽提质粒DNA.结果获得更为简化的方法.用这种方法抽提到的质粒DNA其纯度足以用来转化细菌以及内切酶分析,由于非常简单所以特别适用于重组质粒DNA的筛选以及分子遗传学的实验.

The genetic homology of seven NPV genomes have been studied by restriction enzyme pattern analysis and DNA hybridization utilizing 32P-label-led NPV-DNA as probes. The resits showed that the restriction enzyme patterns of NPV-DNA among five NPV infecting different host species were different, but those between isolates from the same host species were almost the same except for some fragments with different migration rate and some submolar fragments. Detection of genetic homology...

The genetic homology of seven NPV genomes have been studied by restriction enzyme pattern analysis and DNA hybridization utilizing 32P-label-led NPV-DNA as probes. The resits showed that the restriction enzyme patterns of NPV-DNA among five NPV infecting different host species were different, but those between isolates from the same host species were almost the same except for some fragments with different migration rate and some submolar fragments. Detection of genetic homology by DNA hybridi-zation revealed that the major DNA fragments of NPV from the same host species hybridized with homologous probes. No hybridization can be observed between NPV-DNA from different host species. This report suggests that restriction enzyme analysis and molecular hybridization are of value for characterization of this group of viruses.

应用限制性内切酶图谱分析法,结合Southern印迹法和核酸杂交技术,对茶毛虫、棉蛉虫,油桐尺蠖、斜纹夜蛾以及蓖麻蚕等5种昆虫的7株核型多角体病毒DNA,进行了基因组同源性测定。结果表明,不同种昆虫多角体病毒DNA的酶切图谱不相同,DNA片段与不同源的DNA标记探针之间无杂交带出现。而同种昆虫病毒的不同分离株间,除少数DNA片段的电泳迁移率稍有不同,以及出现一些互不相同的亚克分子带之外,它们的DNA酶切图谱基本一致,並且几乎所有片段都可与同种的标记探钟杂交。对一些DNA片段迁移率的改变及亚克分子带出现的原因进行了讨论。

Further studies on HincⅡ polymorphic restriction site 5' to e globin gene of human DNAs from normai Zhuan individuals members of Han families and β-thalassemia patients have been carried out by restriction enzyme analysis. The frequency of the HincⅡ site polymorphism among Zhuan people and β-thalassemia patients were found to be 75%,Which was rather close to that previously reported among Han people. Results from family study showed that the inheritance of the polymorphic site among family members...

Further studies on HincⅡ polymorphic restriction site 5' to e globin gene of human DNAs from normai Zhuan individuals members of Han families and β-thalassemia patients have been carried out by restriction enzyme analysis. The frequency of the HincⅡ site polymorphism among Zhuan people and β-thalassemia patients were found to be 75%,Which was rather close to that previously reported among Han people. Results from family study showed that the inheritance of the polymorphic site among family members seemed to follow Mendel's rule.

本文进一步研究了我国不同民族的正常个体以及β地中海贫血患者θ珠蛋白基因5′侧序列中的多态性HincⅡ位点及其遗传性质。在广西壮族正常个体和β地中海贫血纯合子中,该多态性位点的发生频率均为75%,与正常汉族人测得值相近。家系分析资料表明,该多态性位点完全按照孟德尔规律进行遗传。

 
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