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感染性疾病及传染病
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hepatitis delta
相关语句
  丁型
     Methods Hepatitis delta virus' sequence was obtained from Inner Mongolian patient by using RT-PCR and PCR methods, PET43a was used and His-tag was added at the HDV L-Ag 5′ and 3′ to construct the recombinant expression plasmid, transform the plasmid into host bacterium BL21 and induce it with IPTG.
     方法从内蒙古某丁肝患者血清中提取丁肝病毒,经RT-PCR与PCR,使用PET43a表达质粒及HDVL-Ag5′和3′端引入His标签获得丁型肝炎病毒L-Ag重组表达质粒,转化BL21(Rosetta)宿主菌,IPTG诱导表达。
短句来源
     The serum of 151 cases of gravidas with positive Hepatitis B Virus Marker (HBV -M )and unchangable liver function was detected for Hepatitis Delta Virus Marker (HDV-M ) byEnzyme Immune Assay(ElA). Theresults demonstrated that 24 cases showed HDV-M (+)(24/151, 15. 89% ).
     本文采用酶免疫测定法(EIA),检测长春地区151例乙肝病毒标志物(HBV-M)阳性,肝功能无改变的孕妇血清中丁型肝炎病毒标志物(HDV-M),结果HDV-M(+)共24例,阳性率15.89%(24/151)。
短句来源
     ESTABLISHMENT AND APPLICATION OF DETECTIVE METHOD OF IgM ANTIBODY TO HEPATITIS DELTA VIRUS
     丁型肝炎IgM抗体检测方法的建立和应用
短句来源
     Study on the Inhibition of Hepatitis B Virus (HBV) by Hepatitis Delta Virus (HDV) Ribozyme in Cells
     丁型肝炎病毒(HDV)核酶对乙型肝炎病毒(HBV)抑制作用的研究
短句来源
     wo expression plasmids pETDAg and pETDAg -1 ,which contain the gene of Chinese Henan hepatitis delta antigen were constructed under the T7 Promoter,with and without terminator resepectively .
     将我国河南株丁型肝炎(丁肝)病毒抗原的编码基因,克隆到分泌性原核表达载体pET-12a的T7启动子下游,构建了保留和去除终止子(Terminator)的两个表达载体pETDAg和pETDAg-1。
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  “hepatitis delta”译为未确定词的双语例句
     The plasmid was constructed containing full-length antigenome cDNA of CC-47 that was under control of T7 promoter and joined with hepatitis delta virus ribozyme sequence.
     克隆麻疹病毒CC-47株蛋白N、P、L编码区质粒并置于T7启动子控制下,用4个质粒共转染哺乳动物细胞,在表达T7RNA聚合酶的重组痘苗病毒VTF7-3的作用下进行病毒拯救。
短句来源
     A 27-peptide,a fragment of hepatitis delta antigen(HDAg),was synthesized and used to develop an ELISA method for ihe detection of anti-HD.
     本文选择、设计并合成了含天然HDAg上的部分片段的27肽,用于建立检测抗HD的ELISA方法。
短句来源
     The antibody to hepatitis delta virus (Anti-HD) from the serum positive for HBsAg and Anti-HD was fractionated with ion-exchange chromatography of DE-52 and labelled with horseradish peroxidase (HRP).
     取乙型肝炎患者Delta抗体(抗—HD)阳性血清,经硫酸铵盐析和DE—52柱层析,得到纯化的抗—HD抗体,用辣根过氧化物酶标记。
短句来源
     MOLECULAR CLONING AND SEQUENCING OF A HEPATITIS DELTA ANTIGEN-CODING cDNA FROM CHINA
     中国人丁型肝炎病毒抗原编码区基因的cDNA克隆和序列分析
短句来源
     [Objective] In order to study quality of recombinant antigen of hepatitis delta virus (HDV) and its difference from other HDV antigens.
     [目的]为了研究重组HDV抗原的性质及与其它HDV抗原的差别。
短句来源
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  相似匹配句对
     The Molecular Biology of Hepatitis delta virus
     丁型肝炎病毒的分子生物学研究进展
短句来源
     STUDIES ON CELLULAR IMMUNITY OF DELTA HEPATITIS
     HDV感染者细胞免疫状态的研究
短句来源
     Ischaemia hepatitis
     缺血性肝炎
短句来源
     Hepatitis B Virus
     乙型肝炎病毒
短句来源
     (4)delta ;
     (4)三角洲;
短句来源
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  hepatitis delta
The objective of this study was to estimate the presence of hepatitis delta virus RNA in chronically HBV-infected patients from northern Poland.
      
Hepatitis delta virus infection in chronically HBV-infected patients from northern Poland
      
Serum samples obtained from 289 first-time and 114 repeat donors at the Blood Center of Mongolia (MBC) were tested for serological and molecular markers of hepatitis B virus (HBV), hepatitis C virus (HCV), and hepatitis delta virus (HDV) infections.
      
Using statistical methods we have detected significant similarity between the RNA-binding domain of the hepatitis delta antigen and the HMG box of SRY.
      
The delta antigen of hepatitis delta virus exhibits sequence specific binding to its own RNA and is essential for viral replication.
      
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Anti-hepatitis delta virus(HDV)antibody in various HBsAg positive population has been investigated with Abbott EIA assay.Eighteen of 1004 sera were anti-HDV positive,with a percentage of 1.8.The anti-HDV positive samples of 622 asympt-omatic HBsAg carriers,58 acute hepatitis B patients, 241 chronis hepatitisB patients,and 83 primary liver cancer patients were 4(0.6%),0,4(1.7%),and 10(12.1%)respectively.Detection rate of anti- HDV was not related to the age and sex of the HBsAg positive population....

Anti-hepatitis delta virus(HDV)antibody in various HBsAg positive population has been investigated with Abbott EIA assay.Eighteen of 1004 sera were anti-HDV positive,with a percentage of 1.8.The anti-HDV positive samples of 622 asympt-omatic HBsAg carriers,58 acute hepatitis B patients, 241 chronis hepatitisB patients,and 83 primary liver cancer patients were 4(0.6%),0,4(1.7%),and 10(12.1%)respectively.Detection rate of anti- HDV was not related to the age and sex of the HBsAg positive population. The titers of these anti-HDV antibody in the sera were less than 1:10.The sera reexamination 2 after to 6 months were turned into negative anti-HDV results.The studysuggests that the incidence of hepatitis delta infection is very low in China.

采用Abbott EIA法对各种HBsAg阳性人群血清抗HDV抗体进行了调查,结果在1004份血清标本中检出有18份标本抗HDV抗体阳性,阳性率为1.8%。抗HDV抗体检出率与年龄、性别无关。但在无症状HBsAg携带者、急性乙型肝炎、慢性乙型肝炎(包括慢性活动性肝炎、慢性迁延性肝炎、肝硬化)、肝癌患者血清中,抗HDV抗体检出率有显著性差别,分别为0.6%、0%、1.7%、和12.1%。这些阳性血清中抗HDV抗体滴度<1:10,间隔2~6个月后对部份患者重新采血复查时,抗HDV抗体转为阴性。研究结果提示了低感染率的D型肝炎在我国的存在。

By using EIA and Nucleic Acid Spot Hybridization, we examined HDAg, Anti-HD, and HDV-RNA among 227 patients with hepatitis B in some areas of Fujian province. It was found that four cases were anti-HD positive and one case was HDAg and HDV-RNA positive, with HDVM positive rate of 2. 2% (5/227) . The results confirm that hepatitis delta exixts in Fujian province but with a low infectious rate and dispersive distribution. In clinics, HDVM positive rates of acute hepatitis, chronic hepatitis and...

By using EIA and Nucleic Acid Spot Hybridization, we examined HDAg, Anti-HD, and HDV-RNA among 227 patients with hepatitis B in some areas of Fujian province. It was found that four cases were anti-HD positive and one case was HDAg and HDV-RNA positive, with HDVM positive rate of 2. 2% (5/227) . The results confirm that hepatitis delta exixts in Fujian province but with a low infectious rate and dispersive distribution. In clinics, HDVM positive rates of acute hepatitis, chronic hepatitis and asymptomatic HBsAg carriers respectively were 1. 5% (1/67), 3% (4/135) and 0% (0/25) . The HDVM positive rate of patients with chronic hepatitis is higher than that of HBsAg carriers. It shows that HDV infection is closely related to chronic hepatopathy with HBsAg positive. In addition, it was found that the clinical feature of five patients infected by HDV was superinfection and the HBV replication of most patients were inhibited.

本研究应用酶联免疫(EIA)及核酸斑点杂交法对我省部分地区227例乙型肝炎患者检测了HDAg、抗-HD及HDVRNA。结果4例抗-HD阳性,1例HDAg与HDVRNA均为阳性,HDVM检出率为2.2%(5/227)。证实了丁型肝炎在福建省的存在,但感染率低,呈散在分布。各临床型乙肝患者HDVM检出率分别为:急性肝炎1.5%(1/67);慢性肝炎3%(4/135);HBsAg无症状携带者0(0/25)。慢性肝炎患者HDVM检出率明显高于HBsAg携带者,提示HDV感染与HBsAg阳性的慢性肝病密切相关。并发现本研究5例HDV感染者临床表现均为重叠感染,绝大多数患者HBV复制指标受到抑制。

A 27-peptide,a fragment of hepatitis delta antigen(HDAg),was synthesized and used to develop an ELISA method for ihe detection of anti-HD.It was found that positive anti-HD reaction occurred between the coated 27-peptide and a stored sample of serum which was known anti-HD positive.Absorption test revealed that the synthetic peptide competed with natural HDAg for anti-HD,suggesting that the peptide possessed the antigenicity similar to that of natural HDAg.The antigenicity of the synthetic peptide was...

A 27-peptide,a fragment of hepatitis delta antigen(HDAg),was synthesized and used to develop an ELISA method for ihe detection of anti-HD.It was found that positive anti-HD reaction occurred between the coated 27-peptide and a stored sample of serum which was known anti-HD positive.Absorption test revealed that the synthetic peptide competed with natural HDAg for anti-HD,suggesting that the peptide possessed the antigenicity similar to that of natural HDAg.The antigenicity of the synthetic peptide was quite specific wihtout cross reaction with normal human and mouse sera and with anti-HA.anti-HB and anti-HC sera.Among 300 blood donors,there was only 1 case(0.33%)anti-HD positive with an ALT level 2 times higher than normal.In 41 cases of non-B hepatitis and 52 cases of HAV hepatitis,none was anti-HD positive.In 211 cases of various types of HBV hepatitis,21 were(9.95%)anti-HD positive,among whom 2/82(2.5%)werehealthy HBV carriers,6/43(13.95%)were patients with a-cute icteric hepatitis,6/60(10.00%)were patients of chronic active hepatitis,4/18(22.20%)were patients of severe hepatitis,and 3/8(37.50%)were those with liver cirrhosis.These results were consistent with those in our previous reports.

本文选择、设计并合成了含天然HDAg上的部分片段的27肽,用于建立检测抗HD的ELISA方法。试验结果表明,包被的合成肽抗原与已知抗HD阳性冻存血清呈抗HD阳性反应;它能与天然HDAg竞争抗HD,具天然HDAg上相应片段的抗原活性;该合成肽抗原专一性强,与正常人血清,正常小鼠血清,单纯甲型、乙型、丙型肝炎抗体阳性血清无免疫交叉反应。用建立的ELISA法,检测1992年1~5月的部分献血员及住本科各型肝炎患者血清标本的抗HD。300例献血员中1例抗HD阳性(0.33%),其ALT增高2倍以上。非乙型肝炎41例,甲型肝炎52例,抗HD均阴性。各型乙型肝炎211例,抗HD阳性21例(9.95%)。其中,携带者2/82例(2.5%);急黄肝6/43例(13.95%);慢活肝6/60例(10.00%);重肝4/18例(22.20%);肝炎肝硬化3/8例(37.50%),结果与本科已往报告基本一致。

 
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