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   hepatitis delta 在 基础医学 分类中 的翻译结果: 查询用时:0.17秒
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hepatitis delta
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  丁型
    Study on the Inhibition of Hepatitis B Virus (HBV) by Hepatitis Delta Virus (HDV) Ribozyme in Cells
    丁型肝炎病毒(HDV)核酶对乙型肝炎病毒(HBV)抑制作用的研究
短句来源
    In Vitro Transcription of cDNA of Hepatitis Delta Virus Genomic RNA Ribozyme
    丁型肝炎病毒基因组RNA中核酶区的cDNA的体外转录
短句来源
    Methods Hepatitis delta virus' sequence was obtained from Inner Mongolian patient by using RT-PCR and PCR methods, PET43a was used and His-tag was added at the HDV L-Ag 5′ and 3′ to construct the recombinant expression plasmid, transform the plasmid into host bacterium BL21 and induce it with IPTG.
    方法从内蒙古某丁肝患者血清中提取丁肝病毒,经RT-PCR与PCR,使用PET43a表达质粒及HDVL-Ag5′和3′端引入His标签获得丁型肝炎病毒L-Ag重组表达质粒,转化BL21(Rosetta)宿主菌,IPTG诱导表达。
短句来源
    The Molecular Biology of Hepatitis delta virus
    丁型肝炎病毒的分子生物学研究进展
短句来源
    The Structural Features and Catalytic Acting Mechanism of Hepatitis Delta Virus Ribozymes
    丁型肝炎病毒核酶的结构特点与催化作用机制
短句来源
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  “hepatitis delta”译为未确定词的双语例句
    The plasmid was constructed containing full-length antigenome cDNA of CC-47 that was under control of T7 promoter and joined with hepatitis delta virus ribozyme sequence.
    克隆麻疹病毒CC-47株蛋白N、P、L编码区质粒并置于T7启动子控制下,用4个质粒共转染哺乳动物细胞,在表达T7RNA聚合酶的重组痘苗病毒VTF7-3的作用下进行病毒拯救。
短句来源
    Experimental studies of pockmark ducks infected by hepatitis delta virus
    HDV感染麻鸭的实验研究
短句来源
    INHIBITION OF HEPATITIS DELTA VIRUS GENOMIC RNA RIBOZYME BY ANTI-SENSE OLIGODEOXYNUCLEOTIDES
    反义寡核苷酸体外抗丁型肝炎病毒的初步研究
短句来源
    Molecular Cloning and Sequencing and Comparison of Hepatitis D Antigen(HDAg)-Coding Fragment of Four Chinese Hepatitis Delta Virus Isolates
    中国4株丁型肝炎病毒抗原编码区基因的cDNA克隆与序列分析
短句来源
    Study of the trans-cleaving activity of hepatitis delta virus genomic ribozyme
    丁型肝炎病毒基因组核酶的反式剪切活性
短句来源
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  hepatitis delta
The objective of this study was to estimate the presence of hepatitis delta virus RNA in chronically HBV-infected patients from northern Poland.
      
Hepatitis delta virus infection in chronically HBV-infected patients from northern Poland
      
Serum samples obtained from 289 first-time and 114 repeat donors at the Blood Center of Mongolia (MBC) were tested for serological and molecular markers of hepatitis B virus (HBV), hepatitis C virus (HCV), and hepatitis delta virus (HDV) infections.
      
Using statistical methods we have detected significant similarity between the RNA-binding domain of the hepatitis delta antigen and the HMG box of SRY.
      
The delta antigen of hepatitis delta virus exhibits sequence specific binding to its own RNA and is essential for viral replication.
      
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In order to establish animal model of hepatitis delta virus infection,HDV was inoculated into four DHBV carriers(experimental group) and four normal pockmark ducks(control group).The results showed that two weeks after inoculation,the hepatitis delta antigen(HDAg) in the serum of one duck and the antibody to HDV(anti-HD) in another were detected in DHBV positive ducks.Both HDAg and anti-HD were negative in mormal ducks.It suggested that DHBV may be helpful in the replication and expression of HDV...

In order to establish animal model of hepatitis delta virus infection,HDV was inoculated into four DHBV carriers(experimental group) and four normal pockmark ducks(control group).The results showed that two weeks after inoculation,the hepatitis delta antigen(HDAg) in the serum of one duck and the antibody to HDV(anti-HD) in another were detected in DHBV positive ducks.Both HDAg and anti-HD were negative in mormal ducks.It suggested that DHBV may be helpful in the replication and expression of HDV in pockmark ducks.

为建立丁型肝炎的实验动物模型,分别筛选4只携带DHBV的麻鸭(实验组)和4只正常麻鸭(对照组)进行了HDV实验感染。结果:实验组1只麻鸭在接种后第二周血清中出现HDV抗原,另1只麻鸭血清中检出抗-HD。对照组麻鸭血清中未检出HDV标记物。揭示在麻鸭中DHBV可辅助HDV进行复制和表达。

The cDNA(433-870)fragment of a Hunan isolate of hepatitis delta virus (HDV) was cloned and sequenced from a Chinese patient from Hunan Province, using reverse transcription and polymerase chain reaction. This fragment sequence of the Hunan isolate of HDV shares an 91.3%,94.5%,91.3%,84.5% and 89.3% similarity with the same fragment sequences of the American, the Italian, the French, the Nauru and the Taiwan isolates of HDV, respectively.The first conserved region within this fragment sequence,which is essential...

The cDNA(433-870)fragment of a Hunan isolate of hepatitis delta virus (HDV) was cloned and sequenced from a Chinese patient from Hunan Province, using reverse transcription and polymerase chain reaction. This fragment sequence of the Hunan isolate of HDV shares an 91.3%,94.5%,91.3%,84.5% and 89.3% similarity with the same fragment sequences of the American, the Italian, the French, the Nauru and the Taiwan isolates of HDV, respectively.The first conserved region within this fragment sequence,which is essential for the autocatalytic cleavage of the genomic RNA of HDV,is comletely identical to the American, the Italian,and the French isolates of HDV.

取1例湖南丁型肝炎病毒(HDV)RNA阳性血清,经强变性剂法抽提HDVRNA,逆转录-聚合酶链反应(RT-PCR)扩增,扩增的HDVCDNA片段重组到pUC18质粒中,获得了中国湖南株HDVCDNA(433-870)片段克隆,DNA测序结果显示,该片段(438hp长,包括一端PCR引物25hp)与已知的美国、意大利、法国、诺鲁和中国台湾5株HDVcDNA相同片段比较,同源性分别为91.3%,94.5%,91.3%,84.5%和89.3%,其中与HDVRNA复制密切相关的第一个高度保守区与美国株、意大利株和法国株完全同源。

With pSVLD3 as template, a 139hp cDNA fragment containting the region related to hepatitis delta virus(HDV) genomic RNA ribozyme was amplified by polymerase chain reaction(PCR). The PCR products were cloned into pGEM-3Z.A clone(pHDV108) was obtained. The inserted DNA sequencing was carried out by the dideoxy chain termination method. a two-base-mutation was found. With pHDV108 as template, the precursor RNA of HDV genomic RNA ribozyme was obtained by in vitro transcription using T7 RNA polymerase and its...

With pSVLD3 as template, a 139hp cDNA fragment containting the region related to hepatitis delta virus(HDV) genomic RNA ribozyme was amplified by polymerase chain reaction(PCR). The PCR products were cloned into pGEM-3Z.A clone(pHDV108) was obtained. The inserted DNA sequencing was carried out by the dideoxy chain termination method. a two-base-mutation was found. With pHDV108 as template, the precursor RNA of HDV genomic RNA ribozyme was obtained by in vitro transcription using T7 RNA polymerase and its self-cleavage rate was 71 %.

以质粒pSVLD3为模板,通过聚合酶链反应(PCR)扩增得到1条139bP的cDNA片段,它含有丁型肝炎病毒(HDV)基因组RNA中核酶(Ribozyme)区的cDNA,将此片段克隆到PGEM-32中,经筛选、鉴定,得到克隆株pHDV108,提取质粒后经双脱氧末端终止法测序,发现有2个碱基变异。以该质粒为模板,通过T7RNA聚合酶转录出HDV基因组RNA中核酶区的前体,并观察到其自身裂解产物,自裂率达71%。

 
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