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bone marrow mononuclear cells     
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  骨髓单个核细胞
     Results The expression of CD11a and CD11b on AML blast cells from 51 patients were(36.476±22.641)% and(26.955±12.328)%,respectively,and the expression of CD11a and CD11b on normal bone marrow mononuclear cells were(19.625±12.328)% and(15.513±6.296)%,respectively.
     结果AML细胞上表达的CD11a[(36.476±22.641)%]和CD11b[(26.955±12.328)%]明显高于正常骨髓单个核细胞CD11a[(19.625±12.328)%]和CD11b[(15.513±6.296)%];
短句来源
     METHODS: Bone marrow mononuclear cells from SD rat were grouped as C1 with IMDM/F12 culture medium only and H1 with IMDM/F12+HGF (HGF 20 μg/L).
     方法: SD大鼠骨髓单个核细胞分为单纯IMDM/F12培养基对照组(C1组)和肝细胞生长因子组(H1组,加HGF20μg/L).
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     On days 3, 7, 10, 14, 21 and 28 after BMT, the peripheral blood cells and bone marrow mononuclear cells (BMMNC) were counted, and histology changes of bone marrow were detected.
     分别于BMT后7、14、21和28d检测外周血细胞、骨髓单个核细胞(bone marrow mono-nuclear cells,BMMNC),3、7、10、14、21和28d检测骨髓组织学变化。
短句来源
     Expression of Chk1 and Chk2 in Leukemia Bone Marrow Mononuclear Cells
     细胞周期检测点激酶Chk1和Chk2在急性白血病骨髓单个核细胞中表达的研究
短句来源
     The following factors were measured on day 3,7,10,14,21 and 28 after BMT:peripheral blood cells,bone marrow mononuclear cells (BMMNC), and histology changes of bone marrow.
     分别于BMT后第7、14、21、28d检测外周血细胞、骨髓单个核细胞(bone marrow mono-nuclear cells,BMMNC)、第3、7、10、14、 21、28d检测骨髓组织学变化。
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  骨髓干细胞
     Comparison of the effects of atologous bone marrow mononuclear cells implantation in treating 94 patients with chronic lower limb ischemia at different stages
     自体骨髓干细胞移植治疗慢性下肢缺血94例不同病变分期患者的效果比较
短句来源
     Autologous transplantation of bone marrow mononuclear cells for treating patients with severe lower limb ischemia:a report of 15 cases
     自体骨髓干细胞移植改善下肢严重缺血致运动功能障碍:15例报告
短句来源
     METHODS: Fifteen patients with severe lower limb ischemia were treated by autologous transplantation of bone marrow mononuclear cells.
     方法:应用自体骨髓干细胞移植治疗15例下肢严重缺血。
短句来源
     CONCLUSION:Autologous transplantation of bone marrow mononuclear cells is a simple, safe, and effective method, especially in treating patients with severe lower limb ischemia in which no arterial reconstruction is feasible, or the patients who cannot undertake bridge operation for old, weak or accompanied with other disease.
     结论:自体骨髓干细胞移植治疗下肢缺血性疾病是一种简单、安全、有效的方法,尤其是对于由于下肢远端动脉流出道差,无法进行下肢搭桥或者由于年老体弱或伴发其他疾病不能接受手术搭桥的患者。
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     Donor and recipient inbred isogenic adult (8-10 weeks old) Lewis rats were used as models to mimic intracoronary transplantation of autologous bone marrow mononuclear cells in clinical setting.
     骨髓单个细胞(Bone Marrow Mononuclear Cells,BMMNCs)中含有大量多能干细胞,国外学者发现经体外诱导后骨髓干细胞可分化为肌源性细胞(myogenic cells)或心肌细胞。
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  骨髓单个核细胞的
     Methods Differences in gene expression profile of bone marrow mononuclear cells were compared between primary diagnosis and relapsed/refractory disease in 3 patients with M_ 2a subtype of AML using Agilent human 1B 60mer oligonucleotide microarray.
     方法应用Agilent Human1B寡核苷酸基因芯片,动态检测了3例AML-M2a患者初诊、复发时骨髓单个核细胞的基因表达谱差异。
短句来源
     Methods The gene expression difference of bone marrow mononuclear cells between CML in chronic and in blastic phases was examined with DNA array.
     方法 应用DNA芯片技术 ,对CML慢性期与急变期骨髓单个核细胞的基因表达差异进行了检测。
短句来源
     3.The binding rate of bone marrow mononuclear cells to stromal cell adherence layer descended in different degrees after pre treatment with monoclonal antibodies directed to VCAM 1,Fn,Ln,or Col Ⅳ respectively or VCAM 1 combined with anti Fn,anti Ln or anti Col Ⅳ,respectively,in stromal cell adherence layer.
     ③分别用VCAM1、Fn、Ln和ColⅣ单抗处理骨髓基质细胞贴壁层后,骨髓单个核细胞的粘附率较未加单抗组均有不同程度的下降。
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     ⑦ The apoptosis of bone marrow mononuclear cells of PV patients was less than that of normal controls. PV patients'EEC was negatively correlated with the apoptosis of their bone marrow mononuclear cells ( r=-0.192,P <0.045).
     ⑦PV患者骨髓单个核细胞的凋亡率较正常对照组明显减低 ,PV患者中EEC与骨髓细胞凋亡率呈负相关 (r=- 0 .192 ,P <0 .0 4 5 )。
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     In in vitro culture test, VP-16 upregulated the expression of TRAIL on acute leukemia bone marrow mononuclear cells(P<0.05). Compared with VP-16 alone, the combination of VP-16 with IFNα-2a showed no synergic effects on the expression of TRAIL.
     在体外培养实验中,VP-16上调TRAIL在急性白血病骨髓单个核细胞的表达(P<0.05),VP-16联合干扰素与单独应用VP-16相比,TRAIL的表达水平无差异(P>0.05)。
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  骨髓单个核细胞内
     Study on Negative Regulatory Factors in Bone Marrow Mononuclear Cells of Myelodysplastic Syndromes
     骨髓增生异常综合征骨髓单个核细胞内负调控因子的研究
短句来源
     Methods The GST activity of bone marrow mononuclear cells and LRP expression in 57 acute leukemia patients were detected by the spectrophotometry assay and immuno-cytochemistry(SABC), respectively.
     方法:采用化学比色法检测57例AL患者骨髓单个核细胞内GST活力并同时采用免疫细胞化学(SABC)方法检测LRP表达。
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      bone marrow mononuclear cells
    This investigation uses a system for inducing and detecting anti-tetanus toxoid antibody (anti-TT) synthesis to study specific antibody (Ab) synthesis by bone marrow mononuclear cells (MC).
          
    Previous studies have demonstrated that human bone marrow mononuclear cells secrete a significant amount of IgAin vitro.
          
    In two of six patients no bone marrow mononuclear cells expressing any of these markers were detected.
          
    Alterations in the polyamine levels of the bone marrow mononuclear cells paralleled those occuring in the peripheral blood mononuclear cells in the patients with leukemia.
          
    When ten slides (5 million cells) were examined, the probability of detecting tumor at a concentration of 4 tumor cells per million bone marrow mononuclear cells was 98%.
          
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    We established a semisolid colony culture assay for acute lymphoblastic leukemic cells in vitro. The basic principle of this technique is identical to that used successfully for normal progenitors and acute myeloblastic leukemic cells except that this technique requires feeder cells and low O_2 tension (5%). Bone marrow mononuclear cells depleted of T cells were cultured with media conditioned by T cells in the presence of phytohemagglutinin and irradiated nonadherent mononuclear...

    We established a semisolid colony culture assay for acute lymphoblastic leukemic cells in vitro. The basic principle of this technique is identical to that used successfully for normal progenitors and acute myeloblastic leukemic cells except that this technique requires feeder cells and low O_2 tension (5%). Bone marrow mononuclear cells depleted of T cells were cultured with media conditioned by T cells in the presence of phytohemagglutinin and irradiated nonadherent mononuclear cells, Cells in the colonies had a characteristic pattern, with same cytochemical staining and abnormal karyotype as the original leukemic marrow. The results suggest that the secondary plating efficiency as tested in recloning studies of the primary blast colonies is predictive of the clinical outcome. The assay appears to help detection of small number residual leukemic cells according to the colony pattern. We consider this technique will be valuable in predicting the sensitivity of chemotherapy and in evaluating the quality of purged marrow for autologous marrow transplantation.

    本文采用PHA-TCM及饲养细胞作为刺激条件,并在低氧环境下,建立了急淋L-CFU体外半固体培养方法。22例急性淋巴细胞白血病和恶性淋巴瘤患儿骨髓细胞培养,成功率为91%。集落数与接种细胞数呈线性关系。通过事先去除成熟T淋巴细胞。并根据集落细胞形态、细胞化学染色、染色体及集落细胞传代培养等检查,证明集落细胞属急淋细胞性质。本组试验提示第二代集落得率与临床预后可能有一定关系,同时通过集落培养可协助临床鉴别诊断,并可检测骨髓细胞中白血病细胞残存,为开展自身骨髓移植和体外药敏试验提供一个临床试验的手段。

    T cell clones from human PBL of healthy adults treated by recombinant interleukin-2 (rIL-2) showed inhibitory effect on myeloid progenitor cells growth when the cloned T cells were added into a semisolid medium with a shorter pre-incubation (0.5-4 hours) with bone marrow mononuclear cells (BMMNC).The degree of colony inhibition for GM was well related to the time of T-BMMNC interaction as well as to the number of cloned T cells added into cultures.No substantial difference of the inhibitory effect...

    T cell clones from human PBL of healthy adults treated by recombinant interleukin-2 (rIL-2) showed inhibitory effect on myeloid progenitor cells growth when the cloned T cells were added into a semisolid medium with a shorter pre-incubation (0.5-4 hours) with bone marrow mononuclear cells (BMMNC).The degree of colony inhibition for GM was well related to the time of T-BMMNC interaction as well as to the number of cloned T cells added into cultures.No substantial difference of the inhibitory effect was found between autologous and allogenic T cell clones under same condition.These cloned T cells,with con- tinued rIL-2-stimulation,also showed either good ability of expansion in vitro or lymphokine- activated killer (LAK) activity against NK-sensitive erythroleukemic cell line K562 and NK- resistant human liver cancer cell line H7402 in a 4 hours ~(51)Cr release assay.Phenotypic analysis by flow cytometry indicated that these T clone cells carried T_3,T_8.Tac and DR antigens.These data suggest that cell-to-cell interaction can be the main way at least in the inhibitory effect of rIL-2-activated T clone cells on growth of myeloid progenitor cells,that may be involving cytotoxicity as well as LAK activity and contact inhibition.

    人外周血 T 克隆细胞在 YIL-2持续刺激下具有良好的体外扩增能力和LAK 活性。在半固体琼脂培养体系中,T 克隆细胞抑制骨髓 CFU-GM 形成,抑制效应与 T 细胞数量、T 细胞和骨髓细胞的预孵育时间呈正相关。自体及异体 T 克隆细胞对 CFU-GM 的抑制作用无显著差异。经 FACS 表型分析,这种 IL-2激活的 T 克隆细胞为T_3(+)、T_4(-)、T_8(+)、DR(+)、Tac(+)。

    A CFU-GM-inhibiting activity was found in supernatants of aplastic anemia patients' PHA-induced peripheral blood mononuclear cells and bone marrow mononuclear cells.It was strongly correlated with the γ-IFN concentration and could be eliminated by anti-γ- IFN McAb.With presence of cyclosporin A,the γ-IFN produced by peripheral blood mo- nonuclear cells of the patients studied reduced significantly,followed by a marked decrease in CFU-GM-inhibiting activity in the supernatants just as caused by anti-γ-IFN...

    A CFU-GM-inhibiting activity was found in supernatants of aplastic anemia patients' PHA-induced peripheral blood mononuclear cells and bone marrow mononuclear cells.It was strongly correlated with the γ-IFN concentration and could be eliminated by anti-γ- IFN McAb.With presence of cyclosporin A,the γ-IFN produced by peripheral blood mo- nonuclear cells of the patients studied reduced significantly,followed by a marked decrease in CFU-GM-inhibiting activity in the supernatants just as caused by anti-γ-IFN McAb. All above indicate that cyclosporin A can induce the release of hematopoietic progenitor from γ-IFN-mediated suppression,which may be one of the mechanisms of cyclosporin A in tre- atment of aplastic anemia.

    植物血凝素(PHA)可诱导再生障碍性贫血(AA)病人外周血及骨髓单个核细胞产生 CFU-GM 抑制活性,此活性与上清液中γ-干扰素(IFN-γ)的含量高度相关,并可被γ-干扰素单克隆抗体所中和。加入环孢菌素 A(CsA)(1μg/ml)能有效地抑制 IFN-γ的产生,从而消除其对 CFU-GM 的抑制,提示上述作用可能为 CsA 治疗 AA 的机理之一。

     
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