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restriction analysis identified
相关语句
  限制性酶切鉴定
     Results The cDNA encoding human CK2α′ subunit was obtained from human leukemia cells(HL 60) by RT-PCR. Nde Ⅰ/ Hin d Ⅲ digested PCR product was directly cloned into Nde Ⅰ/ Hin d Ⅲ digested pT7 7 expression vector. The result of restriction analysis identified that this recombinant was successful.
     结果 从人白血病细胞 (HL 60 )中获得了人CK 2α′亚基cDNA ,使用NdeⅠ /HindⅢ双酶切PCR产物和表达载体 pT 7-7进行定向克隆 ,限制性酶切鉴定证明重组获得成功。
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  相似匹配句对
     (N, W) Analysis
     关于点系结构的分析
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     C -- M. S analysis.
     —M. S.
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     Analysis of Language Form of Concept Restriction
     概念限制的语言形式分析
短句来源
     Simultaneity, restriction sites analysis was done.
     同时还对该基因的酶切位点进行了分析。
短句来源
     Two recombinants were identified by restriction analysis.
     重组体的鉴定通过限制酶图谱分析进行。
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  restriction analysis identified
In the experiment, restriction analysis identified the construction of the recombinant plasmid and the inserted sequence was identical with that published on GenBank.
      
Plasmid DNA was isolated from individual pilus-negative transformants, and restriction analysis identified the site of transposon insertion.
      


Objective To construct recombinant expression plasmid of cDNA encoding human protein kinase CK2α′ subunit and study its structure and function.Methods Molecular biology technologies of RT-PCR,directed cloning and DNA sequencing were carried out.Results The cDNA encoding human CK2α′ subunit was obtained from human leukemia cells(HL 60) by RT-PCR. Nde Ⅰ/ Hin d Ⅲ digested PCR product was directly cloned into Nde Ⅰ/ Hin d Ⅲ digested pT7 7 expression vector.The result of restriction analysis identified...

Objective To construct recombinant expression plasmid of cDNA encoding human protein kinase CK2α′ subunit and study its structure and function.Methods Molecular biology technologies of RT-PCR,directed cloning and DNA sequencing were carried out.Results The cDNA encoding human CK2α′ subunit was obtained from human leukemia cells(HL 60) by RT-PCR. Nde Ⅰ/ Hin d Ⅲ digested PCR product was directly cloned into Nde Ⅰ/ Hin d Ⅲ digested pT7 7 expression vector.The result of restriction analysis identified that this recombinant was successful.Results of DNA sequencing for four positive clones indicated that one of them possessed correct sequence of coding region of human CK2α′ subunit,termed as pTCKA′,while the other three contained base mutants.Conclusion The recombinant expressions plasmid of human protein kinase CK2α′ cDNA is successfully cloned.

目的 构建人蛋白激酶CK 2α′亚基cDNA重组表达质粒 ,研究CK 2的结构与功能。方法 采用RT -PCR、定向克隆和DNA测序等一系列分子生物学技术进行本实验。结果 从人白血病细胞 (HL 60 )中获得了人CK 2α′亚基cDNA ,使用NdeⅠ /HindⅢ双酶切PCR产物和表达载体 pT 7-7进行定向克隆 ,限制性酶切鉴定证明重组获得成功。 4个阳性克隆DNA测序结果显示有 1个含有正确插入的人CK 2α′cDNA ,命名为 pTCKA′ ;其余 3个克隆均存在碱基突变。 结论 成功克隆到人CK 2α′亚基cDNA的重组表达质粒。

 
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