助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   conjugated horseradish peroxidase 的翻译结果: 查询用时:0.008秒
图标索引 在分类学科中查询
所有学科
更多类别查询

图标索引 历史查询
 

conjugated horseradish peroxidase
相关语句
  辣根
     After microinjection of cholera toxin B subunit conjugated horseradish peroxidase (CB HRP) into the retrotrapezoid nucleus (RTN), retrogradely labeled neurons were mainly observed in the Klliker Fuse nucleus (KF), the medial parabrachial nucleus (PBM) and the lateral parabrachial nucleus (PBL).
     斜方体后核 (RTN)内微量注入霍乱毒素 β亚单位耦合辣根过氧化酶 (CB HRP)后 ,在脑桥K lliker Fuse核、臂旁内侧核及臂旁外侧核观察到大量HRP标记神经元。
短句来源
  “conjugated horseradish peroxidase”译为未确定词的双语例句
     After electrophysiological studies, a tracer, either wheat germ agglutinin conjugated horseradish peroxidase ( WGA-HRP) or fluoro-gold (FG), was applied iontophorctically to the studied areas on the brain slices. Following incubation for 8-12 hours with artificial cerebrospinal fluid containing 95% O2 and 5% CO2 at 37 ±1℃.
     用电泳法向记录部位导入麦芽凝集素结合的辣根过氧化物酶(WGA-HRP)或荧光金(FG),在和电生理记录相同的条件下孵育脑片8-12h,浸泡固定后做冰冻切片,常规TMB染色,光镜明、暗视野观察,或直接用荧光显微镜观察。
短句来源
     In present study ll adult rabbits were injected with horseradish peroxidase(HRP)or wheat germ agglutinin conjugated horseradish peroxidase(WGA-HRP)in the lumbar cord and the retrogradely labeled cells of the long descending propriospinal neurons(LDPNs)were investigated under LM.
     用成年家兔11只,在脊髓腰膨大部注射HRP或麦芽凝集素结合的辣根过氧化物酶(WGA-HRP),逆行追踪长下行脊髓固有束神经元在颈髓和部分胸髓的分布。
短句来源
     The present investigation is to determine whether this sprouting is reversal after compression of peripheral nerve was relieved. In a rat model of neuropathic pain made by rat sciatic nerve compression,chorela toxin B subunit conjugated horseradish peroxidase (CB-HRP) was used to trace the termination of A-fiber primary afferents and sections were reacted for using tetramethylbenzidine (TMB) as the chromagen.
     为了证实外周神经损伤后形成的这种生芽是否具有可逆性 ,本研究以大鼠坐骨神经压迫造成神经源性痛模型 ,采用跨神经节的霍乱毒素 B亚单位结合辣根过氧化物酶 ( CB-HRP)作为示踪剂 ,四甲基联苯胺 ( TMB)反应呈色追踪 Aβ初级传入末梢。
短句来源
     Methods Cholera toxin B subunit conjugated horseradish peroxidase (CB-HRP) was used to label the neurons innervating Latissimus Dorsi Muscle and Erector Spinae Muscle at the dorsal trunk segment of Bladder Meridian and at non-Meridian.
     方法 用霍乱毒素 B亚单位结合辣根过氧化物酶 ( CB-HRP)对膀胱经经脉线及非经脉线上背阔肌和背最长肌进行逆行示踪研究。
短句来源
  相似匹配句对
     Horseradish peroxidase (HRP) conjugated antibody was produced by treatment of sodium iodate;
     用改良的过碘酸钠法制备辣根过氧化物酶标单抗;
短句来源
     spectrum was338nm,Avidin was conjugated with horseradish peroxidase, and used in BA-.
     最大荧光发射波长为338nm。
短句来源
     Horseradish
     辣根
短句来源
     Determination of Horseradish Peroxidase by Spectrophotometry
     3,3′,5,5′-四甲基联苯胺-H_2O_2-HRP分光光度法测定HRP的研究
短句来源
     The labeled DNA fragment hybridized to the membrane and further conjugated to the horseradish peroxidase linked with streptavidin.
     并与标记的DNA片段杂交;
短句来源
查询“conjugated horseradish peroxidase”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  conjugated horseradish peroxidase
Then, IgG in serum samples was bound to protein A immobilized on a nitrocellulose membrane, and its reactivity to biotinylated concanavalin A was measured with streptavidin-conjugated horseradish peroxidase.
      
In a first experimental group (A) either horseradish peroxidase (HRP) or lectin conjugated horseradish peroxidase (WGA-HRP) was injected into the grafts in order to investigate the origin and the course of regenerated fibres entering the grafts.
      
The ultrastructure and synaptology of the rat imNTS, and its glossopharyngeal afferent terminals, have been examined with cholera toxin-conjugated horseradish peroxidase (CT-HRP) as an anterograde tracer.
      
We also labeled, retrogradely, the neurons projecting to the cardia and the pylorus in the DMV with cholera toxin-conjugated horseradish peroxidase (CT-HRP) to examine their ultrastructural characteristics.
      
Here we examine the synaptic organization of the ceNTS, and its esophageal afferents by using transganglionic anterograde transport of cholera toxin-conjugated horseradish peroxidase (CT-HRP).
      
更多          


Ju and Swanson first named the dorsal part of the anterolateral area of the bed nuclei of the stria terminalis (BST) as oval nucleus (Ov), and found that it was a homogeneous structure rich in neuropeptide-containing neurons and terminals. However, no systemic study has been made about the connections of the Ov.The present experiments were designed to examine the efferent projections of the Ov in the rat. Wheat germ agglutinin conjugated horseradish peroxidase(WGA-HRP) was used as a tracer.Injection of...

Ju and Swanson first named the dorsal part of the anterolateral area of the bed nuclei of the stria terminalis (BST) as oval nucleus (Ov), and found that it was a homogeneous structure rich in neuropeptide-containing neurons and terminals. However, no systemic study has been made about the connections of the Ov.The present experiments were designed to examine the efferent projections of the Ov in the rat. Wheat germ agglutinin conjugated horseradish peroxidase(WGA-HRP) was used as a tracer.Injection of WGA-HRP into the Ov and its adjacent areas resulted in dense anterograde labelling in the posterior part of the lateral hypothalamic area, central nucleus of the amygdala, ventral part of the midbrain central gray, ventral tegmental nucleus, parabrachial nuclei, mesencephalic trigeminal nucleus and locus coeruleus; moderate labelling in the preoptic area, periventricular area of the hypothalamus, arcuate nucleus of the hypothalamus, midline nuclei of the thalamus, medial habenular nucleus, pedunculopontine tegmental nucleus, midbrain reticular formation, dorsal raphe nucleus and the dorsal vagal complex; sparse labelling in the caudal linier nucleus, median raphe nucleus, ventral tegmental area, substantia nigra and intercalated nucleus of medulla oblongata. The results suggest that the Ov may be involved in multiple physiological functions.

本实验选用150~260g的雄性Sprague-Dawley大鼠13只,把WGA-HRP/HRP混合水溶液加压注入一侧终纹床核群前外侧区的卵圆核区域,冰冻切片,TMB法呈色后,在中枢看到顺行标记终末最密集的部位是:下丘脑后部外侧区、中央杏仁核、中脑中央灰质、臂旁核、三叉神经中脑核、蓝斑;比较多的部位是视前区、下丘脑室周区、弓状核、丘脑中线核群、内侧纽核、腹侧背盖核、脚桥背盖核、中脑网状结构、中缝背核以及迷走神经复合体;在线形中缝核、中央上核、腹侧背盖区、黑质,以及延髓中介核,也看到少量标记终末。本工作对卵圆核的传出纤维联系,进行了较全面的观察。

A method for tracing load ncuronal connectivity in combination with physiological studies on brain slices is described. After electrophysiological studies, a tracer, either wheat germ agglutinin conjugated horseradish peroxidase ( WGA-HRP) or fluoro-gold (FG), was applied iontophorctically to the studied areas on the brain slices. Following incubation for 8-12 hours with artificial cerebrospinal fluid containing 95% O2 and 5% CO2 at 37 ±1℃. A number of labelled neurons and fibers or terminals were found...

A method for tracing load ncuronal connectivity in combination with physiological studies on brain slices is described. After electrophysiological studies, a tracer, either wheat germ agglutinin conjugated horseradish peroxidase ( WGA-HRP) or fluoro-gold (FG), was applied iontophorctically to the studied areas on the brain slices. Following incubation for 8-12 hours with artificial cerebrospinal fluid containing 95% O2 and 5% CO2 at 37 ±1℃. A number of labelled neurons and fibers or terminals were found in nearby arcas. The maximum distance of labelling from the application center of the tracer was about 2 an for WGA - HRP and 0.5 mm for FG. The present results show that local neuronal circuits involved in clcctrophysiological studies can be followed on brain slices by tract - tracing techniques.

实验在完成电生理记录的离体脑片上完成。用电泳法向记录部位导入麦芽凝集素结合的辣根过氧化物酶(WGA-HRP)或荧光金(FG),在和电生理记录相同的条件下孵育脑片8-12h,浸泡固定后做冰冻切片,常规TMB染色,光镜明、暗视野观察,或直接用荧光显微镜观察。在导入示踪剂周围可看到明确的逆行标记细胞和顺行标记纤维与终末。WGA-HRP和FG标记物全电泳中心最远距离,分别达2cm和500μm以上。结果表明,用常规神经解剖学方法可以在离体脑片上显示电生理记录部位神经元局部联系回路。

Experiments were done on 23 pentobarbital anesthetized adult cats. 67 unitsrecorded from the parabrachial and Kolliker--Fuse nuclear complex (NPBM-KF) couldbe antidromically activated by electrical stimulation of nucleus raphe magnus (NRM).Of the 67 units, 7 were respiratory related units (6 inspiratory, 1 expiratory). Thesecomprise 8% of the 87 respiratory units examined. The antidromic latencies rangedfrom 0.4 to 2.5 ms. After injection of WGA-HRP (wheat germ agglutinin-conjugated horseradish peroxidase)...

Experiments were done on 23 pentobarbital anesthetized adult cats. 67 unitsrecorded from the parabrachial and Kolliker--Fuse nuclear complex (NPBM-KF) couldbe antidromically activated by electrical stimulation of nucleus raphe magnus (NRM).Of the 67 units, 7 were respiratory related units (6 inspiratory, 1 expiratory). Thesecomprise 8% of the 87 respiratory units examined. The antidromic latencies rangedfrom 0.4 to 2.5 ms. After injection of WGA-HRP (wheat germ agglutinin-conjugated horseradish peroxidase) into the NRM, many retrogradedly labelled neurons were ob-served in the NPBM--KF. These results showed that neurons in the NPBM--KF sent axon-al projections to the NRM, which may be involved in the control of respiration as wellas nociception.

实验在23只苯巴比妥钠麻醉(i.p.30mg/kg)的成年猫上进行。在脑桥呼吸调整中枢(NPBM-KF)共记录到67个单位放电可被电刺激中缝大核(NRM)所逆行兴奋。其中有7个单位为呼吸相关性单位(吸气性6、呼气性1),占脑桥87个呼吸相关性单位总数的8%。逆行兴奋潜伏期在0.4—2.5ms之间,平均1.2ms。中缝大核内微量注入麦角辣根过氧化酶(WGA-HRP)后在NPBM-KF区观察到大量HRP标记神经元。本实验结果表明,发自脑桥呼吸调整中枢神经元的轴突可投射到中缝大核。这一投射通路可能与呼吸及痛觉调节有关。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关conjugated horseradish peroxidase的内容
在知识搜索中查有关conjugated horseradish peroxidase的内容
在数字搜索中查有关conjugated horseradish peroxidase的内容
在概念知识元中查有关conjugated horseradish peroxidase的内容
在学术趋势中查有关conjugated horseradish peroxidase的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社