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fluorescence quantitative pcr assay
相关语句
  荧光定量pcr
     Rapid Detection of Pseudomonas aeruginosa by the Fluorescence Quantitative PCR Assay Targetting 16S rDNA
     16S rDNA用作荧光定量PCR靶基因快速检测铜绿假单胞菌
短句来源
     Methods: The serum samples from 159 cases with 8 different positive models and 67 cases with all negative models were tested by Fluorescence quantitative PCR assay(FQ-PCR) and also by ELISA to compare the results.
     方法:采用荧光定量PCR法和酶联免疫吸附试验(ELISA)对159份HBVm 8种不同阳性模式及67份HBVm全阴模式血清进行HBV DNA检测。
短句来源
     Transfection and fluorescence quantitative PCR assay confirm that a great quantity of newly replicated genomic DNA was detected after the infection of HBV-Brandt linear genomic molecules,indicate that HBV-Brandt linear genome can infect HepG2 cells and replicate strongly in the cells.
     细胞转染和荧光定量PCR分析证明乙肝病毒HBV-Brandt的基因组线性DNA分子可以侵染HepG2细胞,并进行大量复制。
短句来源
     Methods Blood samples were collected from 80 patients with lung cancer,40 benign lung disease patients and 97 healthy controls. Plasma DNA was extracted and purified by"genomic DNA extraction kit". The quantitation of the plasma DNA was performed by real-time fluorescence quantitative PCR assay.
     方法:收集86例肺癌、40例肺部良性疾病及97例健康志愿者的血浆样本,以“微量基因组DNA抽提试剂盒”抽提血浆DNA,以实时荧光定量PCR检测各组血浆循环DNA含量,并作受试者工作特征曲线。
短句来源
     Methods The serum samples from 310 cases of viral hepatitis were tested by Fluorescence quantitative PCR assay(FQ PCR),and also by ELISA as contrast.
     方法 运用荧光定量 PCR(FQ- PCR)和 EL ISA两种方法同时检测了 310份肝炎患者血清 ,并对结果进行了对比分析。
短句来源
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  运用荧光定量pcr
     Methods The serum samples from 310 cases of viral hepatitis were tested by Fluorescence quantitative PCR assay(FQ PCR),and also by ELISA as contrast.
     方法 运用荧光定量 PCR(FQ- PCR)和 EL ISA两种方法同时检测了 310份肝炎患者血清 ,并对结果进行了对比分析。
短句来源
  “fluorescence quantitative pcr assay”译为未确定词的双语例句
     Methods:15 healthy individuals without hepatitis antecedents and 76 patients with chronic HBV infection were included in the study. PBMCs were separated routinely and HBV DNA was measured by fluorescence quantitative PCR assay. Concentrations of cytokines(IFN-γ、TNF-α、IL-4、IL-6、TGF-β1、sIL-2R)in sera were determined by the enzyme-linked immunosorbent assay technique.
     方法 :采用荧光实时PCR技术定量检测 76例慢性乙型肝炎患者和 15例健康者外周血单个核细胞中HBVDNA含量 ,用ELISA法定量检测血浆细胞因子 (IFN γ、TNF α、IL 4、IL 6、TGF β1、sIL 2R)水平。
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Objective To gain in knowledge about the HBV replication and infectivity in patients with hepatitis B and hence to facilitate the selection of approaches to diagnosis,treatment,and assessment of curative effect.Methods The serum samples from 310 cases of viral hepatitis were tested by Fluorescence quantitative PCR assay(FQ PCR),and also by ELISA as contrast.Results In 74 of 80 cases with HBsAg(+),HBeAg(+),HBcAb(+)sample,FQ...

Objective To gain in knowledge about the HBV replication and infectivity in patients with hepatitis B and hence to facilitate the selection of approaches to diagnosis,treatment,and assessment of curative effect.Methods The serum samples from 310 cases of viral hepatitis were tested by Fluorescence quantitative PCR assay(FQ PCR),and also by ELISA as contrast.Results In 74 of 80 cases with HBsAg(+),HBeAg(+),HBcAb(+)sample,FQ PCR results were positive and the load of HBV DNA was 4.16×10 10 copies/ml,with a positive rate of 92.50.In 22 of 68 cases with HBsAg(+),HBeAb(+) and HBcAb(+) samples,the average load was 1.31×10 9 copies/ml,with a positive rate of 32.35%. In 32 of 70 cases of HBsAg(+) and HBcAb(+) samples,the average HBV DNA load was 4.04×10 8copies/ml,with a positive rate of 45.71%.In 2 of 32 cases of HBV M negative,the positive rate of HBV DNA was 6.67%.Conclusion The results showed that HBV DNA could possibly be positive even though it might be negative for HBV M.FQ PCR could be used as another good monitor for the state of HBV infection and its complication.

目的 为了对乙肝患者体内 HBV复制及传染性有更直接地了解 ,亦更有利于对临床 HBV感染的诊断、治疗方案的选择及疗效判定。方法 运用荧光定量 PCR(FQ- PCR)和 EL ISA两种方法同时检测了 310份肝炎患者血清 ,并对结果进行了对比分析。结果 乙肝大三阳患者血清 HBV DNA检出率为 92 .5 % (74/ 80 ) ,平均拷贝数为 4.10× 10 1 0 /ml;小三阳患者血清 HBV DNA检出率为 32 .35 % (2 2 / 6 8) ,平均拷贝数为 1.31× 10 9/ ml;HBs Ag(+ )、HBc Ab(+ )组血清 HBV DNA检出率为 45 .71% (32 / 70 ) ,平均考贝数为 4.0 8× 10 8ml;HBV- M全阴性组血清 HBV DNA检出率仍达6 .6 7% (2 / 32 ) ,平均拷贝数仍达 1.5 4× 10 8/ ml。结论  HBV- M阴性患者仍有 HBV DNA阳性者 ,因此对临床 HBV感染、复制及传染性的判断以及指导治疗 ,除乙肝二对半标志物 EL ISA检测外 ,加做 HBV DNA FQ- PCR检测同样具有重要意义。

clinical serum samples were tested by Fluorescence quantitative PCR assay (FQ PCR),using ELISA as contrast.In 74 HBsAg+/HBeAg+/HbcAb+samples,FQ PCR results were positive,the average level of HBV DNA was 4 16×10 10 ml with a positive rate of 92 50%.In 22 HBsAg+/HbeAb+/HbcAb+samples the average level was 1 31×10 9/ml with a positive rate of 32 35%.In 32 HBsAg+/HBcAb+samples the amount was 4 04×10 8/ml with a positive rate of 45 71%.In 2HBV M(-)sample,the amount of HBV DNA was 1...

clinical serum samples were tested by Fluorescence quantitative PCR assay (FQ PCR),using ELISA as contrast.In 74 HBsAg+/HBeAg+/HbcAb+samples,FQ PCR results were positive,the average level of HBV DNA was 4 16×10 10 ml with a positive rate of 92 50%.In 22 HBsAg+/HbeAb+/HbcAb+samples the average level was 1 31×10 9/ml with a positive rate of 32 35%.In 32 HBsAg+/HBcAb+samples the amount was 4 04×10 8/ml with a positive rate of 45 71%.In 2HBV M(-)sample,the amount of HBV DNA was 1 54×10 8/ml with a positive rate of 6 67%.The results showed that HBV DNA could be positive by FQ PCR,even it may be negative for HBV M,So FQ PCR can be used as another good monitor the state of HBV infection and complication.

本文采用荧光定量聚合酶链反应 (FQ PCR)和ELISA两种方法同时检测了 310份血清 ,并对结果进行了对比分析 ,结果显示 :80例HBsAg (+)、HBeAg (+)、HBcAg (+)组的血清HBVDNA检出率为 92 5 % (74例 ) ,平均拷贝数为 4 10× 10 10 /ml,6 8例HBsAg (+)、HBeAb (+)、HBcAb (+)组血清HBVDNA检出率为32 35 % (2 2例 ) ,平均拷贝数为 1 31× 10 9/ml,70例HBsAg (+)、HBeAb (+)组血清HBVDNA检出率为45 71% (32例 ) ,平均拷贝数为 4 0 8× 10 8/ml;32例HBV M全阴性组血清HBVDNA检出率为 6 .6 7% (2例 ) ,平均拷贝数为 1 5 4× 10 8/ml。结果表明 :HBV -M阴性的病人也可能有HBVDNA阳性 ,因此为临床提供HBV感染、复制及传染性的判断以及指导治疗 ,应选择乙肝两对半标志物检测的同时做PCRHBVDNA定量检测

Objective To explore the effect of hepatitis G virus (HGV) infection on hepatitis B virus (HBV) replication of patients with chronic hepatitis B (CH-B). Methods HGV-RNA in serum, HGV antigen (HGV-Ag) in liver tissues and the amount of HBV-DNA in serum, in liver tissues were detected for 56 patients diagnosed as CH-B by liver biopsy by reverse transcription-polymerase chain reaction (RT-PCR) assay, peroxidase antiperoxidase (PAP) immunohistochemical staining assay and fluorescence quantitative...

Objective To explore the effect of hepatitis G virus (HGV) infection on hepatitis B virus (HBV) replication of patients with chronic hepatitis B (CH-B). Methods HGV-RNA in serum, HGV antigen (HGV-Ag) in liver tissues and the amount of HBV-DNA in serum, in liver tissues were detected for 56 patients diagnosed as CH-B by liver biopsy by reverse transcription-polymerase chain reaction (RT-PCR) assay, peroxidase antiperoxidase (PAP) immunohistochemical staining assay and fluorescence quantitative PCR assay, respectively. Then the relationship between HGV-Ag expression in liver tissues and HGV-RNA expression in serum was analysed and the amount of HBV-DNA in serum and liver tissues from the serum HGV-RNA or liver tissues HGV-Ag positive patients were compared those of the serum HGV-RNA or liver tissues HGV-Ag negative patients, respectively. Results 10(17.9%), 8(14.3%)patients were positive for serum and liver tissues, respectively. HGV-RNA expression in serum was significantly releated to HGV-Ag expression in liver tissues (P<0.01), but some of the liver tissues HGV-Ag negative patients also expressed serum HGV-RNA. There were no significant difference in the amount of HBV-DNA in serum and liver tissues between HGV-RNA or HGV-Ag positive and negative patients (P>0.05). Conclusion HGV infection does not affect HBV replication. Liver is the site of HGV replication, but HGV also replicates in extrahepatic tissues probably. HGV hepatic pathogenicity is at most mild, but further studies still need to be performed.

目的 探讨庚型肝炎病毒 (HGV)感染对慢性乙型肝炎 (CH B)患者乙型肝炎病毒 (HBV)复制的影响。方法 应用逆转录 聚合酶链反应 (RT PCR)、过氧化物酶与抗过氧化物酶复合物 (PAP)法免疫组织化学、荧光定量PCR(FQ PCR)技术对 5 6例CH B患者血清HGV RNA、肝组织HGV Ag、血清及肝组织中HBV DNA含量分别进行了检测 ,并将血清HGV RNA与肝组织HGV Ag的表达、HGV RNA ,HGV Ag阳性与阴性患者HBV DNA含量进行了对比研究。 结果 血清HGV RNA、肝组织HGV Ag阳性分别为 8例 (1 4 3 % )、1 0例 (1 7 9% )。血清HGV RNA阳性与肝组织HGV Ag表达显著相关 (P <0 .0 1 ) ,但部分肝组织HGV Ag阴性患者亦有血清HGV RNA表达。血清HGV RNA、肝组织HGV Ag阳性与阴性患者血清及肝组织中HBV DNA含量均无显著性差异 (P >0 .0 5 )。结论 HGV感染对CH B患者HBV复制无影响。肝脏是HGV的复制场所 ,但可能亦有肝外组织器官中复制。HGV至多具有微弱的致病性 ,但仍然需要进一...

目的 探讨庚型肝炎病毒 (HGV)感染对慢性乙型肝炎 (CH B)患者乙型肝炎病毒 (HBV)复制的影响。方法 应用逆转录 聚合酶链反应 (RT PCR)、过氧化物酶与抗过氧化物酶复合物 (PAP)法免疫组织化学、荧光定量PCR(FQ PCR)技术对 5 6例CH B患者血清HGV RNA、肝组织HGV Ag、血清及肝组织中HBV DNA含量分别进行了检测 ,并将血清HGV RNA与肝组织HGV Ag的表达、HGV RNA ,HGV Ag阳性与阴性患者HBV DNA含量进行了对比研究。 结果 血清HGV RNA、肝组织HGV Ag阳性分别为 8例 (1 4 3 % )、1 0例 (1 7 9% )。血清HGV RNA阳性与肝组织HGV Ag表达显著相关 (P <0 .0 1 ) ,但部分肝组织HGV Ag阴性患者亦有血清HGV RNA表达。血清HGV RNA、肝组织HGV Ag阳性与阴性患者血清及肝组织中HBV DNA含量均无显著性差异 (P >0 .0 5 )。结论 HGV感染对CH B患者HBV复制无影响。肝脏是HGV的复制场所 ,但可能亦有肝外组织器官中复制。HGV至多具有微弱的致病性 ,但仍然需要进一步研究。

 
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