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  “p 53 genes”译为未确定词的双语例句
    Effect of hammerhead ribozyme(RCP)on HBV P gene in vitro
    锤头状核酶RCP对HBV的P基因体外转录物的作用
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    The four RV P gene nucleotide homology was 83.6%-99.8%,the amino acid homology was 87.2%-99%; the sequence interacting! with LC8 was located on 143-148 amino acids remnant bases and was DKSTQT.
    四株病毒P基因核苷酸和氨基酸序列同源性分别为83.6%~99.8%和87.2%~99%,P蛋白与胞浆动力蛋白轻链LC8相互作用的序列位于143~148位氨基酸残基,均为DKSTQT,四株病毒P基因与L蛋白、N蛋白作用位点序列显示未发生影响其生物学功能的变异。
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    Overexpression of the Single mutation Glucose Isomerase(GIG138P)Gene in Streptomyces lividans TK54 and its Genetic Stability
    单点突变葡萄糖异构酶(GIG138P)基因在变铅青链霉菌中的高效表达及其稳定性研究
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    Expression of Green Fluorescent Protein Vector by Promoter sequence of CYP21 Gene and CYP21P Gene
    CYP21和CYP21P基因启动子序列对绿色荧光蛋白基因表达的影响
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    Significance of the YMDD motif mutation of P gene of hepatitis B virus
    乙型肝炎病毒P基因YMDD变异的意义
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  p genes
The objective was to relate the response of the HSP70 and P53 genes to the cessation and the recovery of cardiac muscle cell functions when submitted to ischemia-reperfusion.
      
Comparative study of the expression Rb and p53 genes in human colorectal cancers, color carcinoma cell lines and synchronized hu
      
Comparative study of the expression of Rb and p53 genes in human colorectal cancers, colon carcinoma cell lines and synchronized
      
Concurrent abnormal expression of ERBB-2, EGFR, and p53 genes and clinical disease progression of breast carcinoma
      
These p53 genes were individually mutated at amino acid residues 143, 175, 248 and 273.
      
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A survey of the distribution of ABO,P and Rh blood groups was made among960 primary and middle school students of Tujia Minority in Luota,Xiche andMiaoshi Communes in Longshan county.Slide method was used for the identificationof ABO and P blood groups and direct bromelin method,for Rh blood group.Thedistribution of ABO blood group system in The Minority of Tujia is shown inTable 1.The sequence of the frequencies of phenotypes is A>O>B,AB.As for thegene frequencies,the sequence is O>A>B.In view of...

A survey of the distribution of ABO,P and Rh blood groups was made among960 primary and middle school students of Tujia Minority in Luota,Xiche andMiaoshi Communes in Longshan county.Slide method was used for the identificationof ABO and P blood groups and direct bromelin method,for Rh blood group.Thedistribution of ABO blood group system in The Minority of Tujia is shown inTable 1.The sequence of the frequencies of phenotypes is A>O>B,AB.As for thegene frequencies,the sequence is O>A>B.In view of the frequencies of the pheno-types of P blood group system,P_1 is much more frequent than P_2(Table 2),on thecontrary,the gene frequency P(gene of P_2)is higher than P(gene of P_1)(Table 4).As for the Rh blood group,among 930 investigated individuals,we found only8 phenotypes.The sequence of the frequencies of these phenotypes is CCDee>CcDE>ccDE>CcDee>CCDE>ccDee>CCdE and ccdee(Table 3).The rate of Rh posi-tive is 99.78% and Rh negative,0.22%.The sequence of frequencies of Rh genecomplexes is R' R~e>r>R~o>R~z,while r,r'and r~y are zero(Table 4).Detailedcomparisons are made between our results and those reported by the Shanghai Inst-itute of Biological Products(Table 5 to 8).The probable reason why the frequencyof the phenotype P_1 is much higher than P_2 while the gene frequency is quite thecontrary is discussed in thits paper.

共调查了土家族青年学生960人的 ABO、P 及 Rh 等血型系统,结果表明在 ABO 血型系统中,表现型频率的次序为 A>O>B>AB 型,而基因频率的次序为 O 基因>A 基因>B 基因。P 血型的表现型频率,P_1远高于 P_2,但其基因频率却相反,P_2高于 P_1。在我们调查的930例 Rh 血型中各表现型频率的次序为 CCDee>CcDE>ccDE>CeDee>CCDE>ccDee>CCdE 及 ccdee·Rh阳性占99.78%,Rh 阴性仅占0.22%。基因频率的次序为 R~1>R~2>r>R~0>R~z·r′、r″及置 r~y 为O。我们将调查结果与上海生物制品研究所血型组的调查结果作了较详尽的比较,并指出土家族与各民族间某一血型的表现型分布上的差别以及基因频率的差别的显著性基本上一致,但也有个别不尽相同的情况。此外,本文还讨论了土家族 P 血型 P_1远多于 P_2而 P_1基因频率反较 P_2为小的可能原因。

In order to test the promoter function of two HBV DNA fragments, a soluble cell-free system extracted from Hela celis was used. In the in viiro transcriptional system using the 1.4kb DNA fragment as the templa-te, there were two RNA products whose transcriptional initiation sites were supposed to be at nucleotide 276±5% and 821±5% respectively on the HBV map. The first transcriptional initiation site is identical to the one that is directed by the HBV C gene promoter known before.The rela-tionship between...

In order to test the promoter function of two HBV DNA fragments, a soluble cell-free system extracted from Hela celis was used. In the in viiro transcriptional system using the 1.4kb DNA fragment as the templa-te, there were two RNA products whose transcriptional initiation sites were supposed to be at nucleotide 276±5% and 821±5% respectively on the HBV map. The first transcriptional initiation site is identical to the one that is directed by the HBV C gene promoter known before.The rela-tionship between the location of the second initiation site and the gene open reading frame suggests that the promoter may direct the synthesis of P gene mRNA.The 0.8kb DNA fragment starts from the core structure gene, not in-cluding the regulating sequence. Deducing from the 708±5% nt-long RNA product, the transcriptional initiation site is 588 + 5% on the HBV DNA map. Associated with this RNA start site, there is an ATG codon at po-sition 677 downstream, suggsting that the ATG codon may be a start site of a new open reading frame.

自adr亚型乙型肝炎病毒DNA重组质粒中获得两个DNA片段,用体外转录方法研究启动子的位点。其中1.4kb片段有两个转录产物,其转录起始点分别位于乙型肝炎病毒DNA序列的276±5%位和821±5%位,第一个转录起始点与已报道的乙型肝炎病毒核心抗原基因上游启动子位置一致,第二个转录起始点在888位P基因的起始密码子上游。0.8kb片段自校心抗原结构基因起始密码子ATG以下的序列开始,不含有已知的调控序列,其708±5%核苷酸长的RNA产物,根据其长度计算共转录起始点位于乙型肝炎病毒DNA序列588±5%位,与此位置相关的下游ATG密码子位于677位。

Based on the previous studies with the antisense RNA technique that the best inhibition effect on HBV gene expression carne from the 5'-end and non-coding region of P gene,we designed and synthesized a hammerhead ribozyme(RCP)which targeted at the 5'-end of the P gene at the 2360 site on the HBV ayw genome.Results showed that this ribozyme sucsessfully cleaved its substrate in vitro.The results provide a basis for further study for its blockign effect on HBV gene expression and replication...

Based on the previous studies with the antisense RNA technique that the best inhibition effect on HBV gene expression carne from the 5'-end and non-coding region of P gene,we designed and synthesized a hammerhead ribozyme(RCP)which targeted at the 5'-end of the P gene at the 2360 site on the HBV ayw genome.Results showed that this ribozyme sucsessfully cleaved its substrate in vitro.The results provide a basis for further study for its blockign effect on HBV gene expression and replication in vivo.

本文根据Haseloff和Gerlach发表的锤头状核酶结构,设计合成了针对HBVayw株P基因5'-端2360位点(GUC)的核酶RCP,其作用底物为HPVaywP基因的翻译起始区(2140-2422区段),运用基因克隆结合体外转录的方法,评价了RCP的切割活性,结果表明,RCP在体外成功地切割了长为340个核苷酸的靶RNA分子,这一工作为我们进一步在细胞内评价RCP抑制HBV全基因组复制及其基因表达的研究创造了条件。

 
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