助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   p 53 genes 在 畜牧与动物医学 分类中 的翻译结果: 查询用时:0.134秒
图标索引 在分类学科中查询
所有学科
畜牧与动物医学
肿瘤学
心血管系统疾病
外科学
内分泌腺及全身性疾病
基础医学
临床医学
妇产科学
生物学
更多类别查询

图标索引 历史查询
 

很抱歉,暂未找到该词条在当前类别下的译词。
您可以查看在所有学科的译词。
相关语句
  “p 53 genes”译为未确定词的双语例句
    According to the specific sequence of DHBV P genes, the primers and fluorescent probe were designed and synthesized .
    根据鸭乙型肝炎病毒(duck hepatitis B virus,DHBV)P基因上的保守序列设计并合成引物和荧光标记探针,建立了荧光定量PCR检测方法。
    Cloning and Identifying of P Gene of Newcastle Disease Virus F_(48) E_9 Strain
    新城疫病毒F_(48)E_9株P基因的克隆及鉴定
短句来源
    ClONING OF NP, P AND L GENE OF NEWCASTLE DISEASE VIRUS OF GOOSE ORIGIN AND IDENTIFICATION OF P GENE EXPRESSION
    鹅源新城疫病毒NP、P和L基因的克隆与P基因的表达鉴定
短句来源
    Cloning and sequencing of NP gene,P gene and intergenic region in NP and P gene coding region junction of Newcastle disease virus V4 strain
    新城疫病毒V4株NP和P基因及其间隔区序列的克隆与分析
短句来源
    Pathological Examination of the Chick Embryo Inoculated by NDV F_48E_9 Strain and Cloning,Expression of P Gene of NDV F_48E_9 Strain
    新城疫病毒F_48E_9株接种鸡胚的病理学观察及其P蛋白基因的克隆与表达
短句来源
更多       
查询“p 53 genes”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  p genes
The objective was to relate the response of the HSP70 and P53 genes to the cessation and the recovery of cardiac muscle cell functions when submitted to ischemia-reperfusion.
      
Comparative study of the expression Rb and p53 genes in human colorectal cancers, color carcinoma cell lines and synchronized hu
      
Comparative study of the expression of Rb and p53 genes in human colorectal cancers, colon carcinoma cell lines and synchronized
      
Concurrent abnormal expression of ERBB-2, EGFR, and p53 genes and clinical disease progression of breast carcinoma
      
These p53 genes were individually mutated at amino acid residues 143, 175, 248 and 273.
      
更多          


According to published P protein gene sequence of NDV,we designed and synthesized a pair of primers PU/PL.A 1272bp fragment was amplified from F 48 E 9 strain by means of RT_PCR and cloned into the pMD18_T vector.The recombinant plasmid was proved to be true by enzyme analysis and PCR.In order to further identify the credibility of the gene,the 5' terminal sequence of the cDNA was obtained by sanger's sequencing technique.Compared the obtained gene sequence of F 48 E 9 strain...

According to published P protein gene sequence of NDV,we designed and synthesized a pair of primers PU/PL.A 1272bp fragment was amplified from F 48 E 9 strain by means of RT_PCR and cloned into the pMD18_T vector.The recombinant plasmid was proved to be true by enzyme analysis and PCR.In order to further identify the credibility of the gene,the 5' terminal sequence of the cDNA was obtained by sanger's sequencing technique.Compared the obtained gene sequence of F 48 E 9 strain with the corresponding sequence of La Sota strain by DNASIS software,the homology of the nucleotide sequence was 83.7%.As a result,we obtained complete P gene clone of NDV F 48 E 9 strain.

根据NDVP蛋白己知基因序列设计合成了一对引物PU/PL ,利用RT_PCR扩增出了F4 8E9株P基因 12 72bp的片段 ,将该片段克隆到pMD18_TVector上 ,并对所得到的重组质粒进行酶切分析、PCR鉴定 ,结果证明我们得到了这个基因片段的阳性重组子。为了更充分证实所得阳性质粒的可靠性 ,采用Sanger’s双脱氧末端终止法对阳性重组子从 5’端进行核苷酸序列测定 ,获得了F4 8E9株P基因片段 5’端的基因序列 ,利用DNASIS分析软件 ,将F4 8E9株与LaSota株的相应序列进行比较 ,其核苷酸序列同源性为 83 7%。至此 ,我们获得了F4 8E9株P基因的全长克隆

NP, P and L gene of Newcastle disease virus of goose origin were amplified and cloned into pGEM-T easy vector and then subcloned into pCI-neo expression vector respectively, the positive clones were identified by enzyme cutting, PCR and sequencing. GFP reporter gene was inserted into the downstream of recombinant expression plasmid of P gene, which of stop codon was deleted. The experiment of transfection of P and GFP recombinant plasmid on COS-1 cells and CEF showed that GFP gene...

NP, P and L gene of Newcastle disease virus of goose origin were amplified and cloned into pGEM-T easy vector and then subcloned into pCI-neo expression vector respectively, the positive clones were identified by enzyme cutting, PCR and sequencing. GFP reporter gene was inserted into the downstream of recombinant expression plasmid of P gene, which of stop codon was deleted. The experiment of transfection of P and GFP recombinant plasmid on COS-1 cells and CEF showed that GFP gene expressed, and this demonstrated that P gene was also expressed. This research may be helpful for further study of reverse genetics and functional genome of NDV of goose origin.

将鹅源新城疫病毒的NP、P和L基因通过RT PCR方法从尿囊液中扩增后分别克隆进pGEM Teasy载体 ,再分别亚克隆到真核表达载体pCI neo上 ,通过酶切、PCR和测序验证克隆正确。利用P基因开放性阅读框 (ORF)上靠近终止密码上游的AgeI位点 ,将报告基因绿色荧光蛋白 (GFP)基因克隆进P基因真核表达重组质粒 ,分别转染COS 1细胞和CEF细胞 ,在倒置荧光显微镜下可见到绿色荧光 ,表明GFP基因已得到表达 ,由此证明P基因也已得到表达。鹅源新城疫病毒NP、P和L基因的克隆成功 ,为即将进行的鹅源新城疫病毒的反向遗传操作以及功能基因组研究打下基础

The P gene of Newcastle disease virus encodes two accessory proteins including the V and W protein by means of inserting of non-template nucleotides and this is called RNA editing. The mechanism of RNA editing and the functions of the produced accessory proteins especially of the V protein of Newcastle disease virus were reviewed in this article.

新城疫病毒的P基因转录过程中在第484特定位置插入非模板G碱基发生RNA编辑现象 ,产生两种非结构蛋白 ,即V和W蛋白。文章综述了副黏病毒 (主要是新城疫病毒 )P基因RNA编辑的机理、产生的非结构蛋白与机体内干扰素之间的相互作用及相关研究进展

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关p 53 genes的内容
在知识搜索中查有关p 53 genes的内容
在数字搜索中查有关p 53 genes的内容
在概念知识元中查有关p 53 genes的内容
在学术趋势中查有关p 53 genes的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社