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   p 53 genes 在 临床医学 分类中 的翻译结果: 查询用时:2.043秒
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  “p 53 genes”译为未确定词的双语例句
    Objective To evaluate the application value of gene chip technology in the detection of Hepatitis B Virus genotypes and p gene YMDD mutations,and analyse the clinical status of different genotype cases.
    目的评价基因芯片技术在HBV基因型及HBVP基因YMDD变异检测中的应用价值,并对不同基因型病例的临床情况进行分析。
短句来源
    Methods: HBV DNA P genes were amplified by PCR and HBV YMDD mutations were identified by direct sequence compared with Genebank standard sequence. HBV P gene sequence analysis was conducted in 107 chronic hepatitis B (CHB) patients.
    方法:应用聚合酶链反应(PCR)扩增HBV DNAP基因区,通过直接测序方法与Genebank标准序列对比,对107例慢性乙肝(CHB)患者血清进行P区测序。
短句来源
    The strategy to increase the sensitivity of polymerase chain reaction in the detection of hepatitis B virus P gene
    提高乙型肝炎病毒P基因区聚合酶链反应检测阳性率的策略
短句来源
    Detection of Polymorphism of Hepatitis B Virus P Gene by DNA Microarray
    芯片检测HBV DNA聚合酶区基因突变及其临床应用
短句来源
    Development of a New Method for Detecting YMDD Mutations of P Gene of Hepatitis B Virus and Study of Clinical Practical
    HBV YMDD变异检测方法的建立及临床应用的初步研究
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  p genes
The objective was to relate the response of the HSP70 and P53 genes to the cessation and the recovery of cardiac muscle cell functions when submitted to ischemia-reperfusion.
      
Comparative study of the expression Rb and p53 genes in human colorectal cancers, color carcinoma cell lines and synchronized hu
      
Comparative study of the expression of Rb and p53 genes in human colorectal cancers, colon carcinoma cell lines and synchronized
      
Concurrent abnormal expression of ERBB-2, EGFR, and p53 genes and clinical disease progression of breast carcinoma
      
These p53 genes were individually mutated at amino acid residues 143, 175, 248 and 273.
      
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Objective To establish a technique of short segment PCR ELISA for detecting the variation locus at C section of P gene of HBV to analyze the relationship between lamivudine and HBV resistance to lamivudine in the course of therapy. Methods Variation of P gene of HBV polymerase was determined by short segment PCR ELISA according to the recombined plasmid which was acquired by the site directed mutagensis. Results Sixty blood samples of patients of hepatitis B were determined and their wild type...

Objective To establish a technique of short segment PCR ELISA for detecting the variation locus at C section of P gene of HBV to analyze the relationship between lamivudine and HBV resistance to lamivudine in the course of therapy. Methods Variation of P gene of HBV polymerase was determined by short segment PCR ELISA according to the recombined plasmid which was acquired by the site directed mutagensis. Results Sixty blood samples of patients of hepatitis B were determined and their wild type is "ATG". Eight of them had mutated, and it is proved by sequencing that there was variation of YMDD. Among the eight mutants, four turned to "GTG", one turned to "ATT", the other three turned to "ATC". It was also proved that there was bounce of quantity of HBV DNA and variation of liver function. Conclusion The technique of short segment PCR ELISA for detecting the variation of HBV YMDD is more sensitive and spend only one fifth time in comparision with common PCR.

目的 建立短片段PCR ELISA技术 ,检测HBVP基因C区变异位点 ,以了解拉米呋啶治疗过程中与HBV耐药有关突变株的产生。方法 通过定点诱变获得的重组质粒作为标准对照 ,采用短片段PCR ELISA方法对拉米呋啶治疗的 6 0例乙肝患者血清标本进行HBV聚合酶P基因的变异检测 ,同时观察其HBVDNA定量、肝功能指标的变化。结果 所测 6 0份血样中有 8份检出突变株 ,经测序证实YMDD变异存在 ,其中 4例由ATG变为GTG ,1例变为ATT ,3例变为ATC ,并出现HBVDNA定量反跳及肝功能的变化。结论 短片段PCR ELISA法监测乙肝病毒YMDD变异 ,其方法与一般PCR相比 ,时间仅为其五分之一 ,且具有更好的特异性

Objective To establish a convenient method for detecting YMDD mutations in polymerase gene (P gene) of hepatitis B virus (HBV) and to evaluate the effect of lamivudine on these mutations. Methods Sera of 108 patients with chronic hepatitis B treated with lamivudine and showed positive HBV DNA (PCR) before treatment were detected for YMDD mutations in HBV by method of combining nested/mismatched PCR with analysis of restriction fragment length polymorphism (RFLP). Results The above method can...

Objective To establish a convenient method for detecting YMDD mutations in polymerase gene (P gene) of hepatitis B virus (HBV) and to evaluate the effect of lamivudine on these mutations. Methods Sera of 108 patients with chronic hepatitis B treated with lamivudine and showed positive HBV DNA (PCR) before treatment were detected for YMDD mutations in HBV by method of combining nested/mismatched PCR with analysis of restriction fragment length polymorphism (RFLP). Results The above method can be applied to distinguish well YMDD wild and mutant HBV; In patients treated with lamivudine, HBV DNA turned negative in 63 cases(58 3%). 23(21 3%) and 22(20 4%) showed YMDD wild and mutant HBV, respectively. Conclusion Nested/mismatched PCR-RFLP can be applied to identifying YMDD mutant HBV clinically; lamivudine may lead to YMDD mutations in P gene of HBV in some chronic hepatitis B patients treated with lamivudine.

目的 建立简便易行的检测乙型肝炎病毒 (HBV)多聚酶基因 (P基因 )上YMDD变异株的方法 ,评价运用拉米夫定治疗慢性乙型肝炎患者对此变异的影响。方法 采用套式 /错配聚合酶链反应 (PCR)结合限制性片段长度多态性 (RFLP)分析技术对 10 8例治疗前HBVDNA(PCR)阳性正在拉米夫定治疗中的慢性乙型肝炎患者的HBVYMDD变异情况进行检测。结果 运用上述技术能有效区分HBVYMDD野生株和变异株 ;上述 10 8例患者经拉米夫定治疗后 ,HBVDNA阴转者 63例 (5 8 3 % ) ,HBVYMDD野生株和变异株分别为 2 3例 (2 1 3 % )和 2 2例 (2 0 4% )。结论 建立的套式 /错配PCR -RFLP分析技术可用于HBVYMDD变异株的临床监测 ;拉米夫定治疗慢性乙型肝炎患者可导致部分患者的HBV多聚酶基因上YMDD发生变异

Objective To increase the sensitivity of polymerase chain reaction(PCR) in the detection of hepatitis B virus(HBV) P gene. Methods To reduce the blight of the mismatch between primers and their targets, some methods were applied such as choosing primers, modifying primers, optimizing PCR conditions, decreasing the anneal temperature etc. Results The sensitivity of PCR in the detection of HBV P gene increased from 81。7%(67/82) to 92。7%(76/82, P<0。05) by using methods of amending primers...

Objective To increase the sensitivity of polymerase chain reaction(PCR) in the detection of hepatitis B virus(HBV) P gene. Methods To reduce the blight of the mismatch between primers and their targets, some methods were applied such as choosing primers, modifying primers, optimizing PCR conditions, decreasing the anneal temperature etc. Results The sensitivity of PCR in the detection of HBV P gene increased from 81。7%(67/82) to 92。7%(76/82, P<0。05) by using methods of amending primers and decreasing the anneal temperature. Conclusion Comprehensive methods including choosing primers, modifying primers, optimizing PCR conditions and decreasing the anneal temperature should be used to increase the sensitivity of PCR in the detection of variable region of HBV DNA。

目的 提高乙型肝炎病毒 (HBV)DNAP基因区聚合酶链反应 (PCR)检测的阳性率。方法 采用优化PCR反应条件 ,降低退火温度以及采用 3′末端碱基游移兼并引物 ,减少引物与模板引物结合区的错配对PCR的影响等多种方法 ,对常规PCR检测HBVDNAP基因区阴性的病人血清 ,再进行PCR检测。结果 通过降低退火温度及减少 3′末端错配 ,PCR检测阳性率由 81 7% ( 6 7/ 82 )增加至 92 7% ( 76 / 82 ,P <0 0 5 )。结论 综合采用优化PCR反应条件 ,降低退火温度和采用 3′末端碱基游移兼并引物等方法 ,可提高基因高突变区PCR检测阳性率。

 
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