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necrosis     
相关语句
  坏死
     The Experimental Study of Deproteinized Bone with pcDNA3.1/VEGF165 Facilitating the Repair of Early Avascular Necrosis of Femoral Head of Rabbit
     脱蛋白骨复合VEGF真核表达质粒促进兔股骨头早期缺血坏死修复的实验研究
短句来源
     The Clinical and Experimental Study on Early Treatment in Avascular Necrosis of Femoral Head
     股骨头缺血性坏死早期治疗的临床及实验研究
短句来源
     Experimental Study of Pravastatin on Early Stage of Steroid-induced Avascular Necrosis of the Femoral Head
     普伐他汀对激素性股骨头缺血坏死早期干预的实验研究
短句来源
     Treatment of Rabbit Femoral Head Necrosis Model by Tissue Engineering Bone: An Experimental Study
     组织工程骨修复兔股骨头坏死模型的实验研究
短句来源
     Fundamental Studies on Tissue Necrosis Mechanism of Avulsed Skin Flap after Skin Avulsion
     皮肤撕脱伤撕脱皮瓣坏死机理的研究
短句来源
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  坏死的
     Intravenous Large Dosages of Vitamin C for Treatment of Experimental Myocardial Necrosis in Rabbits
     静注大剂量维生素C对实验性心肌坏死的治疗
短句来源
     Morphologic Observation on Spinal Cord Necrosis in Rabbits Caused by Graded Impact Force
     不同打击量所致家兔脊髓坏死的形态学观察
短句来源
     Experimental Study on Drug Prophylaxis and Treatment of Incipient Acute Tubular Necrosis
     药物防治初发期急性肾小管坏死的实验研究
短句来源
     An experimental study of the laser fluorescence spectrum in determinations of ischemia intestine necrosis
     激光荧光光谱法判断缺血性肠坏死的实验研究
短句来源
     CT Diagnosis of Avascular Necrosis of the Femoral Head
     股骨头缺血性坏死的CT诊断
短句来源
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  细胞坏死
     By hypoxia/hypoglycemia for 5 hours and reoxygenation for 24 hours,the percentage of apoptosis was 49.85%(P< 0.01),the percentage of necrosis was 11.49%(P< 0.01), the intracellular Ca2+concentration 298.11 nmol/L(P< 0.01) and the rate of LDH release 60.35%(P< 0.01).
     神经细胞缺氧/缺糖5h后再给氧24h时,细胞凋亡百分率为49.85%(P<0.01),细胞坏死百分率为11.49%(P<0.01),细胞内Ca2+浓度为298.11nmol/L(P<0.01),LDH的释放率为60.35%(P<0.01)。
短句来源
     Rate of necrosis in groupⅡ12 is (1.12±0.56)%,Rate of necrosis in groupⅡ72 is (1.46±0.39)%,Rate of necrosis in group Ⅳ12 is (5.33±1.05)%.
     Ⅱ12细胞坏死率为(1.12±0.56)%,Ⅱ72细胞坏死率为(1.46±0.39)%,Ⅳ12细胞坏死率为(5.33±1.05)%。
短句来源
     Topiramate could reduce the cellular necrosis in hippocampi:the percentage of cellular necrosis was significantly reduced from(72.1±13.4)%to (20.6±7.2%) in the CA3 region(P< 0.05);
     托吡酯可减少海马区细胞坏死:CA3区坏死细胞百分比由(72.1±13.4)%降至(20.6±7.2)%(P<0.05);
短句来源
     TNF α(1 000 ng/ml)induced K 562 cells'necrosis.
     TNF- α(1 0 0 0 ng/ml)诱导 K5 6 2 细胞坏死
短句来源
     Results: 2.5 μmol/L of As2S3 could induce most of cell HL 60 necrosis and a small part of cell Apoptosis within 18 h,7.5 μmol/L As2S3 could induce apoptosis of human leukemia cell line HL 60 within 8 hours.
     结果:2.5μmol/LAs2S3作用18h后诱导HL60小部分细胞凋亡和大部分细胞坏死7.5μmol/LAs2S3作用8h后可引起HL60细胞凋亡。
短句来源
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  枯斑
     Influence on the numbers of TMV necrosis spots and activities of resistant related enzymes of treating Nicotiana tabacum cv. Xanthi-nc with Jinggangmycin A
     井冈霉素A处理珊西烟后对TMV枯斑数目和抗性相关酶的影响
短句来源
     T-02 was suggested to be amosaic strain(TuMV-jm)showing yellow mosaic symptoms and T-05 a necrotic strainTuMV-jn)showing system necrosis in most cultivars of rape.
     T-05为枯斑系统坏死症状,称为坏死型株系(TuMV-jn)。
短句来源
     The response types could be classified into mosaic leaves, necrosis lesions.
     发现对TMV侵染的响应可分为花叶和枯斑反应 2种类型。
短句来源
     At 24 hours after infiltration of the elicitor into tobacco (cv. W38) leaves at dosage of 10 nmol/L, a bright blue autofluorescence became visible under UV light in a ring of cells surrounding the necrosis tissue, which indicated compounds derived from the phenylpropanoid pathway were accumulated during HR. By Evans blue and trypan blue staining, it was observed that cell death occurred only in infiltrated tissue and was completed within 20 hours after treatment.
     结果是 ,以 10nmol/L激发子溶液注射处理W38烟草叶片 ,HR枯斑周围 5mm宽组织在UV光下呈现蓝色荧光 ,对处理部位进行Evansblue染色测定结果是至 2 0h处理部位细胞全部死亡 ;
短句来源

 

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      necrosis
    popularis, but a lower salt-induced increase of MDA and permeability was observed and lighter leaf necrosis occurred.
          
    Tumor necrosis factor (TNF)-α-converting enzyme (TACE) is the major protease responsible for processing pro-TNF-α from membrane-anchored precursors to secreted TNF-α.
          
    Their hepatopancreas were swollen and pale, accompanied by fatty degeneration, fatty necrosis of hepatocytes, and atrophy of the pancreas.
          
    Histopathological slices showed that it could promote tumor tissue necrosis and decrease the density of blood vessels.
          
    Spotty necrosis occurred in some local liver tissues.
          
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    Chinese rape, varieties of Brassica campestris and B. chinensis and many cruciferous crops including radishes, but except varieties of B. oleracea, are generally affected by a mosaic disease, causing serious losses amounting occasionally to a death rate of over 90%, while an infection rate of over 30% is not uncommon. The symptoms involved were vein-clearing, yellow or green veinbanding, typical mosaic, rugosity of leaf lamina, dwarfing and rosetting. They may appear in different combinations. Flower stalks...

    Chinese rape, varieties of Brassica campestris and B. chinensis and many cruciferous crops including radishes, but except varieties of B. oleracea, are generally affected by a mosaic disease, causing serious losses amounting occasionally to a death rate of over 90%, while an infection rate of over 30% is not uncommon. The symptoms involved were vein-clearing, yellow or green veinbanding, typical mosaic, rugosity of leaf lamina, dwarfing and rosetting. They may appear in different combinations. Flower stalks may not come out or are shortened, deformed and twisted. Flowers appeared pale yellow and become abortive, or forming only small and twisted seed pod, which are sometimes spotted with black necrosis, and bearing small and deformed seeds. When the winter temperature is low, diseased plants may succumb first, forming missing hills. Late infections may result only in vein-banding of the upper leaves and partial abortion of the seeds. On several varieties of radish, mostly of the green type, which apparently are more resistant, only fine mottling appears on the leaves while infected. On varieties of B. napella, another oil-bearing group, the symptoms are entirely different (plate 1, fig. 5), appearing as systemic bright yellow spots on the leaves. Necrotic flecking can eventually be found at the center of these spots. Necrotic streaks and spots develop on the flower stalks and seed pods. Dwarfing and distortion occur on leaves, stalks and pods, and death may even be resulted from severe infections. However, this species showed certain degree of resistance to the disease in the form of late infection and the lower incidence of the disease. The viruses from different host plants are cross-inoculable, although some strains do not infect B. napella. None of them can infect the cabbage group by sap inoculation. 3 viruses were identified: differentiated by their physical properties and the host reactions they induced (Tables 1 and 2 for the viruses 1 and 2): 1. Thermal inactivation below 70℃. 2. Producing necrotic local lesions only on N. tabacum var, "Nungling 400" …… virus 1 2. Producing necrotic local lesions and systemic mottling on the same host……virus 2 1. Thermal inactivation above 90℃., necrotic local lesions on N. glutinosa…… virus 3 The first two viruses can be subdivided into 2 strains each by their ability to infect B. napella. Virus 1 is considered to be a strain of Brassica virus 2 or the Turnip mosaic virus; from the type, it differs in not infecting the cabbage group. Virus 2 is identified as a strain of Cucumis virus 1 or the Cucumber mosaic; virus; from the type, it differs in having lower thermal inactivation point (55℃.), lower dilution end point (1: 1,000—3,000); and shorter longevity invitro (2 days), in producing local lesions on tobacco, and in that the typical strain of Cacumis virus 1 gives only a partial protection to this strain on tobacco. The 3rd virus was less studied. It shows affinity to Nicotiana virus 1 or the tobacco mosaic virus. A limited test showed that the first virus was the only virus found present in the field in 1957. An analysis of 17 isolates of virus from the Chinese rape, exhibiting various syndromes of symptoms in the field, gave an Unanimous result in host reactions, indicating that these isolates belong to a single virus. Virus 1 was found to be easily transmitted by sap, by aphids, including peach aphid (Myzus pcrsicae), false cabbage aphid (Rhopalosiphum pseudobrassicae), and cotton aphid (Aphis gossypii). In insect relationship, the virus belongs to the nonpersistent group, a single feeding will enable an active transmission of about 20 minutes. Peach aphid seems to be an effective vector that 2 or more viruliferous aphids per plant are capable of 100% transmission. The virus is not seed-transmitted. Fresh residue of diseased plant in the soil may cause occasional infection of the replanted plants. The virus can infect several cruciferous weeds, but, among them, only Rorippa montana (wall.) Small grows all the year round and possesses a perennial crown. It is also foundto be commonly infected in the nature. Rubbing with infected sap or using aphid as vector, the virus from this weed can easily be transmitted to the Chinese rape, producing the typical mosaic symptoms. This weed, therefore, seems to be capable of beoming a source of inoculum for the primary infection of the Chinese rape.

    南京及华东地区的油菜与十字花科蔬菜普遍发生花叶病。病原病毒經抗性(失毒温度、稀释終点、体外保毒期)測定和寄主反应測定,认为可以分作三大类型:分别为芸苔病毒2号,黄瓜病毒1号与烟病毒1号的株系。前两种病毒都能为害油菜、大白菜、白菜、芥菜、蘿卜等,但不为害甘蓝型的蔬菜,并都能引起菠菜和茼蒿的花叶,和在烟上引起局部枯斑。第一型在烟农特400号和心叶烟上不引起花叶,而第二型在烟和心叶烟上都能引起系統性花叶。第一型不为害心叶烟,而第三型可在心叶烟上引起小枯斑。第一型与凌立、楊演的油菜花叶病毒相近似,而和范怀忠、柯冲从十字花科蔬菜中所分离出的分离物1号最为相象。与裘維蕃、王祁楷在白菜上所发現的孤丁病毒的差别,在于不能侵害甘蓝类的蔬菜。第二型病毒与范怀忠、柯冲的分离物3号相近似,但与典型的黃瓜花叶病毒仍具有一定的差异。这些病毒在油菜上所引起的症状却沒有明显的差別。1、2两个类型的病毒都有一部分能为害早生朝鮮型的油菜,引起系統性黄斑及枮斑,病重的也能早期死亡。大田中的油菜病株的病毒,大多属于第一型病毒。病毒主要由桃蚜和蘿卜蚜传染,病毒系非持續性的,蚜虫一次吸毒后,传病期不超过20分钟。两个以上的桃蚜传病率就可达100%。种子...

    南京及华东地区的油菜与十字花科蔬菜普遍发生花叶病。病原病毒經抗性(失毒温度、稀释終点、体外保毒期)測定和寄主反应測定,认为可以分作三大类型:分别为芸苔病毒2号,黄瓜病毒1号与烟病毒1号的株系。前两种病毒都能为害油菜、大白菜、白菜、芥菜、蘿卜等,但不为害甘蓝型的蔬菜,并都能引起菠菜和茼蒿的花叶,和在烟上引起局部枯斑。第一型在烟农特400号和心叶烟上不引起花叶,而第二型在烟和心叶烟上都能引起系統性花叶。第一型不为害心叶烟,而第三型可在心叶烟上引起小枯斑。第一型与凌立、楊演的油菜花叶病毒相近似,而和范怀忠、柯冲从十字花科蔬菜中所分离出的分离物1号最为相象。与裘維蕃、王祁楷在白菜上所发現的孤丁病毒的差别,在于不能侵害甘蓝类的蔬菜。第二型病毒与范怀忠、柯冲的分离物3号相近似,但与典型的黃瓜花叶病毒仍具有一定的差异。这些病毒在油菜上所引起的症状却沒有明显的差別。1、2两个类型的病毒都有一部分能为害早生朝鮮型的油菜,引起系統性黄斑及枮斑,病重的也能早期死亡。大田中的油菜病株的病毒,大多属于第一型病毒。病毒主要由桃蚜和蘿卜蚜传染,病毒系非持續性的,蚜虫一次吸毒后,传病期不超过20分钟。两个以上的桃蚜传病率就可达100%。种子不传病。殘留在土壤內的新鮮病根,可以传病。蔊菜在自然情况下可以感染病害。病株上病毒可由蚜虫传至油菜而誘发典型的花叶病,且具有終年生长及終年发病的特性,可能成为初期发病的病毒来源。

    Two series of experiments were carried out on 109 young female albinorats.In the first series the spinal cord was hemisected at the mid-thoraciclevel and examined from 1 hour to 240 days after operation for degenerationand regeneration.In the second series the spinal cord was transected at themid-thoracic level.In order to influence the condition of scar tissue,segmentsof degenerated sciatic nerve were used as grafts for auto- and homo-trans-plantations.In the latter case some of the grafts were stored in ice-box...

    Two series of experiments were carried out on 109 young female albinorats.In the first series the spinal cord was hemisected at the mid-thoraciclevel and examined from 1 hour to 240 days after operation for degenerationand regeneration.In the second series the spinal cord was transected at themid-thoracic level.In order to influence the condition of scar tissue,segmentsof degenerated sciatic nerve were used as grafts for auto- and homo-trans-plantations.In the latter case some of the grafts were stored in ice-box (2℃)for 2 days before grafting.After 7 to 120 days the effects of different trans-plantations on regeneration were studied.Sections of the specimens were pre-pared by a modified Glees' silver impregnation method,and some were coun-terstained with Mallory's triple stain.One hour after injury,some of the cut ends of the nerve fibers swelledand showed increased argyrophilia.Six hours later typical Cajal's retractionballs were found at the cut ends of some fibers.Later on they detached them-selves from the axons and broke into fragments and disappeared gradually.Twenty-four hours after injury some ascending fibers showed Walleriandegeneration.On the third day a great number of them had degenerated andthe maximum degeneration was observed on the fifth day.The fragments ofWallerian degeneration persisted for a long time.Twenty-four hours after the operation a few regenerating fibers withgrowth cones at their tips were observed.It was found that as the regenera-tion proceeded some of the regenerating fibers degenerated and disappeared.Transplantation of segments of degenerated nerves resulted in alteration of the structure of scar tissue and enabled regenerating fibers grow along thelong axis of the cord.Most regenerating fibers that grew into the transplantscame from the dorsal root,only a small portion came from the cord proper.The number of regenerating fibers in the transplants varied with their posi-tion,the occurrence of necrosis within them and density of the surroundingscar.It was found that auto-transplantation had the best effect,homo-trans-plantation after cold storage came next and homo-transplantation without coldstorage produced the least effect.It was found that the regenerative capacity of the spinal cord was com-paratively slight and that the nerve fibers did not regenerate uniformly.Mostregenerating fibers in the scar tissue came from dorsal funiculus,some origi-nated from the ventral horn cells,only few of them came from the graymatter and the fasciculus proprius.Other fibers regenerated only occasionally.Transection of spinal cord resulted in paralysis of the hindlimbs but reflexactivity remained intact.In animals whose spinal cord had been hemisected,the coordination of locomotion was recovered in a few days.It is evident,that the central nervous system of albino rat possesses remarkable compensatorypower.The factors causing the failure of regeneration in the central nervoussystem are discussed.

    1.本报告系采用体重70—100克较幼的雌性大白鼠,在第6—8胸椎平面半切断脊髓,手术后1小时至240天分期取出脊髓,用改良的Marsland,Glees及Erikson的石蜡镀银法染色,也有部分用Mallory三色法复染,系统观察脊髓内轴突的溃变和再生。另外用已溃变的坐骨神经进行自体、同种和冷藏后再行同种移植的方法来填补脊髓的缺损,观察不同的移植方法和移植块对脊髓再生的影响。2.脊髓内少数纤维的断端,在损伤后1小时初显改变,6小时已形成Cajal式典型的缩回球,24小时已有少数缩回球和轴突分离,经破裂成碎片而逐渐消失。从15天以后,各期都看到少数再生纤维的尖端形成嗜银性很强的类似缩回球的肿大,此现象在240天仍继续发出。3.切断纤维的两端都能形成缩回球,其形状、部位和纤维的直径有密切的关系。棒状缩回球主要由粗纤维形成,离切口较远;球状缩回球大部分紧靠切口的边缘,由细纤维的断端所形成。4.损伤后24小时,少数上行纤维开始华氏溃变,第3天有显著的增加,第5天已达最高峰。华氏溃变产生的碎片的消失极为缓慢,在240天仍有部分碎片散布在结节状肿大的纤维之间。5.带有生长锥的分枝再生纤维,在手术后24小时开始形成,但数...

    1.本报告系采用体重70—100克较幼的雌性大白鼠,在第6—8胸椎平面半切断脊髓,手术后1小时至240天分期取出脊髓,用改良的Marsland,Glees及Erikson的石蜡镀银法染色,也有部分用Mallory三色法复染,系统观察脊髓内轴突的溃变和再生。另外用已溃变的坐骨神经进行自体、同种和冷藏后再行同种移植的方法来填补脊髓的缺损,观察不同的移植方法和移植块对脊髓再生的影响。2.脊髓内少数纤维的断端,在损伤后1小时初显改变,6小时已形成Cajal式典型的缩回球,24小时已有少数缩回球和轴突分离,经破裂成碎片而逐渐消失。从15天以后,各期都看到少数再生纤维的尖端形成嗜银性很强的类似缩回球的肿大,此现象在240天仍继续发出。3.切断纤维的两端都能形成缩回球,其形状、部位和纤维的直径有密切的关系。棒状缩回球主要由粗纤维形成,离切口较远;球状缩回球大部分紧靠切口的边缘,由细纤维的断端所形成。4.损伤后24小时,少数上行纤维开始华氏溃变,第3天有显著的增加,第5天已达最高峰。华氏溃变产生的碎片的消失极为缓慢,在240天仍有部分碎片散布在结节状肿大的纤维之间。5.带有生长锥的分枝再生纤维,在手术后24小时开始形成,但数量极少。有一些分枝的再生纤维继续生存150天或更长。在再生过程中经常有少数再生纤维发生溃变而消失。6.移植已溃变的神经能够部分改变脊髓内瘢痕的结构,使再生纤维沿着脊髓纵轴生长,有利于纤维的再生;但是移植块常引起较严重的炎性反应,被致密的结缔组织所包绕,阻碍再生纤维长入移植块。7.长入移植块的再生纤维,极大部分来自后根,少数东自后索,也有一些可能来自灰质。移植块内再生纤维的数量是随着移植块所在的部位、放置的方向、周围瘢痕的致密程度及是否发生局部坏死而转移。8.自体移植的效果最好,淋巴细胞的浸润比较轻,再生纤维最丰富。冷藏后同种移植的次之。同种移植由于在移植块中心常发生局部坏死而引起较严重的炎性反应,影响了再生纤维的长入。9.脊髓内神经纤维的再生能力较微弱而且是不一致的,瘢痕内的再生纤维以后索的上行纤维来的较多,前角细胞次之,极少的来自灰质和固有束,其他纤维的再生更为少见。脊神经的后根常有大量的再生纤维长入瘢痕。10.脊髓侧索在损伤后15天初显收缩,随后更为显著。其收缩的程度在近侧段和远侧段相似,在90天受伤侧的侧索的厚度只有正常侧的一半左右。11.所有全切断脊髓和移植动物,除呈现反射活动外,都没有发现后肢的协调运动;但是全部脊髓半切断动物在手术后3—7天都已经恢复正常的协调步态。可见大白鼠的中枢神经系是具有高度的代偿机能。12.中枢神经系再生失败的原因曾加以讨论。

    1. The different methods of transmission of caprine pleutopneumonia have been investigated with the seed materials derived from the sporadic cases from Sinkiang, Shansi and Shantung Provinces. The results were briefly summaried as follows: (1) By subcutaneous inoculation, only local swelling and elevation of body temperature were found in a small number of goats, no pneumonic changes were seen from autopsy. (2) By intramuscular inoculation, the tested goats showed massive edema and necrosis at the site...

    1. The different methods of transmission of caprine pleutopneumonia have been investigated with the seed materials derived from the sporadic cases from Sinkiang, Shansi and Shantung Provinces. The results were briefly summaried as follows: (1) By subcutaneous inoculation, only local swelling and elevation of body temperature were found in a small number of goats, no pneumonic changes were seen from autopsy. (2) By intramuscular inoculation, the tested goats showed massive edema and necrosis at the site of inoculation and the surrounding area of the muscles, and nopulmonary lessions have been seen from autopsy. (3) By intravenous injection, one of the two goats was infected and hepatizafion of the lungs was observed. (4) By direct contact, the latent period is usually over 20 days. Both adult and kids might be infected. (5) By intrathoracic inoculation, the morbidity is approximately 72%. The latent period is uncertain. (6) By inhalation of atomized material through nostrils, the goat usually showed symptom 5—18 days later. (7) By intratracheal injection, the morbidity is about 95%, and the incubation period is 3—7 days. When the goats were injected in a standing position, the hepatized areas were usually seen on the right lung; when injected first in a recumbent position and then in standing position, the hepatized areas were usually seen on both sides of the lungs. 2. The virulent lung tissue of goat infected with caprine pleuropneumonia is non-patho-genic to sheep, rabbit and guinea pigs. 3. The infected lung tissue retained its virulence for 20 days in room temperature (16℃) and for 24 days in cold stroage (2℃) in 50% glycerin saline. 4. The frozen-dried lung tissue kept at 2—8℃ retained its virulence for 49 months. 5. The M. I. D. of fresh lung tissue for goat through intratracheal injection is about 0.000004 gin., while the frozen-dried lung tissue is about 0.004 gm. in equivalent for fresh lung tissue.

    1.曾以山傳肺組織乳劑液或胸水,作對山羊之各種途徑感染試驗,其結果如下: (1)皮下注射法:僅使少數羊呈現體温及局部腫脹反應,但不引起山傳特具的肺臟病理變化。 (2)肌肉注射法:可引起局部及隣近的筋肉腫脹發炎,呈濕性壞死,重者陷於死亡,但肺臟無變化。 (3)靜脈注射法:可發生疾病並引起肺臟肝變。 (4)人工接觸法:在接觸20多天後,大多數羊均可發病,雖小羔亦難倖免。 (5)胸內注射法:發病率為了27%,潛伏期長短很不一致,肺的病變多限於局部。 (6)噴霧戚染法:有一部分羊經過噴霧感染後5—18天發病,並引起肺臟肝變。 (7)氣管注射法:發病率高達95%以上,潛伏期大多數為3—7天,用站立姿勢注射法,病變多在右肺葉,如採用站立仰臥姿勢兼用注射法,則病變遍於左右兩葉。 2.山傅的強毒肺組織液,不能使緬羊、家免及海猪等試驗動物致病。 3.新鮮肺組織在50%甘油鹽水中,置室温(16℃)20天,冰箱(2℃)124天,仍有致病力,如再延長時間,則失去致病力。但病原組織凍乾後,置普通冰箱保存49個月又13天,仍有致病力;2年以後,其發病毒價約減低10倍。新鮮病原組織小至0.000004克仍可致病。凍乾後毒力約減低1000倍...

    1.曾以山傳肺組織乳劑液或胸水,作對山羊之各種途徑感染試驗,其結果如下: (1)皮下注射法:僅使少數羊呈現體温及局部腫脹反應,但不引起山傳特具的肺臟病理變化。 (2)肌肉注射法:可引起局部及隣近的筋肉腫脹發炎,呈濕性壞死,重者陷於死亡,但肺臟無變化。 (3)靜脈注射法:可發生疾病並引起肺臟肝變。 (4)人工接觸法:在接觸20多天後,大多數羊均可發病,雖小羔亦難倖免。 (5)胸內注射法:發病率為了27%,潛伏期長短很不一致,肺的病變多限於局部。 (6)噴霧戚染法:有一部分羊經過噴霧感染後5—18天發病,並引起肺臟肝變。 (7)氣管注射法:發病率高達95%以上,潛伏期大多數為3—7天,用站立姿勢注射法,病變多在右肺葉,如採用站立仰臥姿勢兼用注射法,則病變遍於左右兩葉。 2.山傅的強毒肺組織液,不能使緬羊、家免及海猪等試驗動物致病。 3.新鮮肺組織在50%甘油鹽水中,置室温(16℃)20天,冰箱(2℃)124天,仍有致病力,如再延長時間,則失去致病力。但病原組織凍乾後,置普通冰箱保存49個月又13天,仍有致病力;2年以後,其發病毒價約減低10倍。新鮮病原組織小至0.000004克仍可致病。凍乾後毒力約減低1000倍,其最小發病量一般為0.004克。

     
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