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anti-p
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  抗p
     Cloning and Expression of Fab Genes of Anti-p185 Monoclonal Antibody 5E12
     抗p185单抗5E12Fab段基因的克隆及表达
短句来源
     Results: One strain(2C4)of IgG1 Kappa anti-P34H McAb was harvested.
     结果:获得1株(2C4)IgG1 Kappa型抗P34H M cAb。
短句来源
     Results ① The positive rate of anti-p68 antibody was 67.8% (124/183) in RA, 14.9% (17/114) in other rheumatic diseases and none in normal controls (0/81).
     结果①183例RA患者中,抗p68抗体的阳性率为67.8%(124/183),114例非RA风湿病患者中阳性率为14.9%(17/114),81名正常人中无一例阳性(0/81)。
短句来源
     Preparation and cell activity determination of bispecificity monoclonal antibody of anti-p~(21) protein/anti-CD_3
     抗P~(21)蛋白/抗CD_3双特异性单克隆抗体制备及细胞毒活性研究
短句来源
     Metabolism of ~(125)I labeled anti-p185 antibodies in vitro and their biodistribution in vivo
     ~(125)I标记的抗p185抗体的体外代谢与体内生物学分布
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  抗人p
     Anti-P185~(erbB2) scFv-Fc-IL-2 modulates tumor cell surface molecules and activates immune effector cells
     抗人P185~(erbB2) scFv-Fc-IL-2调变肿瘤细胞表面分子和激活免疫效应细胞
短句来源
     The MTT assay demonstrated that the chimeric antibody could inhibit the growth of SKBR3 cells overexpressing p185 erbB2 . Conclusion: The anti-p185 erbB2 mouse/human chimeric antibody that was expressed in CHO-dhfr - cells can bind to p185 erbB2 specifically and inhibit proliferation of SKBR3 cells overexpressing p185 erbB2 . It has a potential application in biotherapy of cancer.
     MTT比色法检测表明嵌合抗体对过表达p185 erbB2 的乳腺癌细胞SKBR3的生长有抑制作用。 结论 :CHO dhfr-细胞表达的人 -鼠嵌合抗体可与p185 erbB2 特异性结合 ,并可抑制高表达p185 erbB2 的肿瘤细胞生长 ,表明抗人p185 erbB2 人 -鼠嵌
短句来源
     Eukaryotic expression and biological activities of anti-p185~(erbB2) mouse/human chimeric antibody
     抗人p185~(erbB2)人-鼠嵌合抗体真核表达载体的构建表达及活性测定
短句来源
     Investigation on the anti-cancer activities of anti-p185 monoclonal antibodies in vitro
     抗人p185单克隆抗体的体外直接抗癌效应
短句来源
     Objective: To elucidate the mechanisms by which anti-P185~(erbB2)scFv-Fc-IL-2 (HFI) modulates tumor surface molecules and activates immune effector cells in vitro. Methods: Ovarian Cancer Cell Line SKOV3 and human peripheral blood monocyte peripheral blood mononuclear cells (PBMC) were treated with HFI.
     目的将抗人P185erbB2scFv-Fc-IL-2融合蛋白(HFI)作用于人卵巢癌细胞株SKOV3细胞和人外周血单个核细胞(PBMC),通过体外实验阐明HFI调变肿瘤细胞表面分子和激活免疫效应细胞的抗肿瘤机制,为HFI临床应用提供实验依据。
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  “anti-p”译为未确定词的双语例句
     RESULTS: A total of 20 patients (29.4%) were detected positive for serum anti-P53 antibody.
     结果:68例PHC血清P53抗体阳性率29.4%(20/68).
短句来源
     The expression of phosphorylated p44/42 MAPK protein was evaluated by the immunoblotting technique using anti-p44/42 phospho-MAPK antibody.
     利用p44/42磷酸化抗MAPK抗体的蛋白免疫印迹法测定磷酸化p44/42 MAPK蛋白表达。
短句来源
     AIM: To analyze the level of serum anti-P53 antibody in primary hepatic carcinoma (PHC) as well as its correlation with clinical characteristics.
     目的:分析原发性肝癌(primary hepatic carcinoma,PHC)患者血清P53抗体与临床特征的关系.
短句来源
     Mul- tivariate analysis showed that only remote me- tastasis was independently associated with the positive status of serum anti-P53 antibody (OR = 4.185, P = 0.022).
     多因素分析显示只有肝外转移与P53抗体阳性有关(OR=4.185,P=0.022).
短句来源
     Preparation, identification and application of anti-P450scc antibody
     兔抗大鼠神经甾体合成关键酶P450scc抗体的制备、特性鉴定及应用
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  anti-p
DNA binding affinity (ΔTm) of the tested compounds did not correlate with either their anti-P.
      
p24 antigen (p24 Ag) is usually not found in patients in the asymptomatic phase of HIV infection, mainly because it is bound to anti-p24 antibodies (p24 Ab), with which it forms immune complexes.
      
However anti-glycolipid and anti-p35, -p21,-p18 IgG antibodies were detected in a small subset of patients, mainly corresponding to more severe courses of the disease.
      
falciparum genomic DNA expression library with mouse convalescent anti-P.
      
The activation of NF-κB was detected by immune fluorescence cytochemistry and Western blotting with anti-p65 antibody.
      
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The schizonts ( merozoites ) of plasmodium falciparum were iodinated by 125I-lactoperoxidase method and the labelled parasites extracted in Triton X-100. The extract was then either precipitated by mouse anti-P falciparum monoclonal antibody (McAb)94D1 or purified through an immunoabsorbent column prepared with the same monoclonal antibody.The results showed that this monoclonal antibody precipitates three polypeptides of 55kd,48 kd and 25 kd in molecular weight, which are identical to those purified by...

The schizonts ( merozoites ) of plasmodium falciparum were iodinated by 125I-lactoperoxidase method and the labelled parasites extracted in Triton X-100. The extract was then either precipitated by mouse anti-P falciparum monoclonal antibody (McAb)94D1 or purified through an immunoabsorbent column prepared with the same monoclonal antibody.The results showed that this monoclonal antibody precipitates three polypeptides of 55kd,48 kd and 25 kd in molecular weight, which are identical to those purified by using immunoabsorbent technique.

用~(125)Ⅰ—乳过氧化物酶法标记恶件疟原虫裂殖体(子),TritonX—100提取疟原虫表面标记抗原。抗恶性疟单克隆抗体9_4D_1与上述恶性症原虫膜抗原提取物作免疫沉淀分析,并用单克隆抗体9_4D_1制备免疫吸附剂,通过亲和层析自恶性疟原虫提取物中分离纯化相应的靶抗原。结果表明,免疫沉淀的靶抗原分子量为55kd,48kd和25kd的多肽,与亲和层析纯化出的靶抗原基本一致.

Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus ( PrC-RSV...

Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus ( PrC-RSV ) , subgroup C. Both MCAs im-munoprecipitated p19 from 35S-methionine-labeled RPL-40 or RAV-1,but not RAV-O infected chi-cken embryo fibroblasts ( CEF ) . They can be used to differentiate exogenous from endogenous ( RAV-O ) infection either in an indirect antibody ELISA or by immunoprecipitation. A third MCA, 6AL42 ( IgG2a isotype), reacted with exogenous viruses at an antibody titer up to 1,000-fold higher than with endogenous subgroup E, RAV-O virus in indirect ELISAs. MCA 6AL42 immunoprecipitated p27 and the group-specific antigen precursor protein, pr76, from cells infected with RPL-40, RAV-1 or RAV-O. A double antibody sandwich ELISA, using 6AL42 as the primary binding antibody and conjugated rabbit anti-p27 as the reporter antibody, differentiated between endogenous RAV-O and exogenous p27 antigen in undiluted albumen samples.

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb-6AL42可与P~(2...

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb-6AL42可与P~(27)和pr~(76)(群特异性抗原的前体蛋白)发生免疫沉淀。试验用McAb-6AL42作为第一抗体和兔抗P~(27)血清的过氧化物酶标记物作为第二抗体(指示抗体)建立了双抗体夹心ELISA试验。用这种ELISA试验可区分未经稀释的卵蛋白中RAV-O和RPL-40群特异性抗原。

The free or carrier-linked immunogens were prepared from three synthetic peptides correspondeing to the sequence of hypatitis B surface antigen.These immunogens were injected into rabbits,and most them elicited an antipeptide response.Antisera against P122-148(ayw)and P122-148(adw)were rective to native HBsAg as shown by AUSAB test,in which the anti-P122-148(adw) sera showed higher reactivity than other antipeptide antibodies ever reported.Structural analysis indicated that immunogens containing the immunodominant...

The free or carrier-linked immunogens were prepared from three synthetic peptides correspondeing to the sequence of hypatitis B surface antigen.These immunogens were injected into rabbits,and most them elicited an antipeptide response.Antisera against P122-148(ayw)and P122-148(adw)were rective to native HBsAg as shown by AUSAB test,in which the anti-P122-148(adw) sera showed higher reactivity than other antipeptide antibodies ever reported.Structural analysis indicated that immunogens containing the immunodominant regions of native proteins would be ideal candidates for preparation of efficient vaccines by synthetic methods.

我们将三种乙型肝炎表面抗原的合成肽段在联结载体或不接载体的情况下,分别制备成4种免疫原对兔子进行了免疫。大部分兔子都产生了抗肽抗体。其中抗P_(122-48)(auw)与抗P_(122-148)(adw)都能与天然HBsAg反应,而抗P_(122-148)(adw)的抗HBs活力比文献报道的都高,通过结构分析表明:含有天然蛋白上免疫显性区域的免疫原是合成疫苗的理想选者。

 
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