A high performance liquid chromatography method for the simultaneous determination of inosine 5'-monophosphate (IMP) and guanosine 5'-monophosphate (GMP) was developed on a WAX - 1 (4. 0 × 50mm) with the mobile phase of 0.05mol/L monobasic potassium phosphate and UV detection at 260nm.
5'-Nucleotides include Uridine 5'-monophosphate(5'-UMP), Guanosine 5'-mono-phosphate(5'-GMP), Cytidine 5'-monophosphate(5'-CMP), Adenosine 5'-mono-phosphate(5'-AMP), They have wide use in the industry of food.
The mean membrane potential of 10μmol/L ACh-induced hyperpolarization was-13.67±0.98mV(n=116),the mean electrical conductance increased 2.31%(n=10),and the hyperpolarization induced by ACh could be blocked by atropine sulphate(1μmol/L) and 2'-deoxyadenosine 5'-monophosphate (100nmol/L),and were accompanied by a decrease of membrane potential.
Kinetic Mechanism of Allosteric Regulation of Muscle Glycogen Phosphorylase b by Adenosine 5"-Monophosphate
The above compounds were found to be main radiolysis products upon irradiation of thymidine and thymidine 5"-monophosphate.
The main effect of binding of H2TMPyP4 adenosine 5'-monophosphate and to poly(dA-dT)2 is shown to be an increase in the fluorescence lifetime from 4.6 ns in the solution to 8.3 and 12.3 ns, respectively.
Pulse radiolysis of chlorogenic acid (CGA) as well as CGA and 2'-deoxyguanosine-5'-monophosphate (dGMP) has been carried out in aqueous solution using pulse radiolytic technique.
It is generally accepted that adenosine-3'5'-monophosphate mediates the effects of catecholamines in the myocardial cell.George et al.