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virus induced
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  病毒诱导
     EPSTEIN-BARR VIRUS INDUCED MALIGNANT TRANSFORMATION OF IMMORTALIZED HUMAN EPITHELIAL CELLS
     Epstein-Barr病毒诱导永生化人上皮细胞恶性转化
短句来源
     Epstein-Barr Virus Induced Thymus Malignant T Cell Lymphoma
     EB病毒诱导胸腺恶性T细胞淋巴瘤的研究
短句来源
     VIRUS INDUCED GENE SILENCING--The Presumable Mechanism of Plant Resistance to Virus
     病毒诱导基因沉默——植物抗病的可能机制
短句来源
     How DNA and RNA viruses have silence plant gene expression were examined and the advantages and disadvantages of VIGS in determining gene function and the future trend of virus induced gene silencing were discussed.
     阐述了DNA和RNA病毒诱导植物基因沉默的机理,同时讨论了利用病毒诱 导的基因沉默(VIGS)鉴定植物基因功能的优缺点和将来的发展趋势。
短句来源
     Method: More than two hundreds Chinese medicinal herb extracts were screened for antiviral activities against influenza A/PR/8/34 (H1N1) virus using 3-(4,5-dimerthylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay for virus induced cytopathic effect (CPE) in a primary screening.
     方法:用四唑氮化合物(MTS)测定流感病毒诱导细胞病变(CPE)方法,初筛200余种中草药提取物抗A型流感病毒活性。
短句来源
  肝炎病毒性
     Intrahepatic gene expression profiles and alpha-smooth muscle actin patterns in hepatitis C virus induced fibrosis
     丙型肝炎病毒性肝纤维化的肝内基因表达谱与α-平滑肌肌动蛋白着色模式
短句来源
  “virus induced”译为未确定词的双语例句
     METHODS The pathological model was established It based on FM 1M 12 E 11 flu virus induced pneumonia fatal to mice.
     方法 采用流感病毒FM1M12 E11鼠肺适应株攻击用可致小鼠死亡的肺炎建立的强毒模型 ,研究抗小鼠流感病毒的作用。
短句来源
     Conclusions H5N1 avian flu virus induced brain damage in Macaca rhesus, appeared non-suppurative encephalitis. The result further confirmed that H5N1 avian influenza virus is progressively adapting to mammals and become more neurologically virulent.
     结论H5N1禽流感病毒导致恒河猴大脑损害,出现非化脓性脑炎,进一步证实了H5N1禽流感病毒对哺乳类日渐适应并且表现出越来越强的神经毒性。
短句来源
     Applications of Plant Virus Induced Gene Silencing for Gene Function Studies
     植物病毒诱导的基因沉默在基因功能研究中的应用
短句来源
     However, BGC823 cells were resistant to H-1 virus induced cytotoxicity.
     而BGC82 3则对H 1病毒的细胞毒作用抵抗。
短句来源
     Production of Antibodies against preS Antigen of Hepatitis B Virus Induced by Gene Vaccine Encoding the Fusion Protein Composed of Human Interleukin-2 and preS Antigen
     人白细胞介素-2与乙型肝炎病毒前S抗原融合蛋白基因疫苗诱导抗前S抗体产生
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  virus induced
Agroinoculation experiments indicated that both viruses induced symptomatic infections in tomato and tobacco, whereas neither virus induced disease symptoms in pepper, common bean, small sugar pumpkin, African eggplant, or Arabidopsis.
      
UV-inactivated virus induced more interferon than infectious virus.
      
Louis encephalitis virus induced pathology in cultured cells
      
Transmissible gastroenteritis virus induced apoptosis in swine testes cell cultures
      
The confocal laser scanning microscope is an effective tool for the study of the three-dimensional structures of plant virus induced inclusions.
      
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Mouse interferon mRNA,extracted from NDV (Newcastle,disease virus)-induced Lpacecells,has been translated in whole HeLa cells,and the production of mouse interferon in HeLa cells during translation was reproducible with an average yield of 72 units/ml.However,mouse interferon mRNA was no longer translated in HeLa cells in antiviral state after treatment of human interferon.This phenomenon may be involved in the mechanism of antiviral action of interferons.

从NDV病毒诱导的Lpa细胞中分离的小鼠干扰素mRNA能在完整的HeLa细胞中翻译,而且翻译时小鼠干扰素在HeLa细胞中的生产有较好的重复性,平均产量为72单位/毫升。然而在用人的干扰素处理HeLa细胞后,处在抗病毒状态的HeLa细胞就不能再翻译小鼠的干扰素mRNA。这一现象可能与干扰素的抗病毒机制有关。

Previous works from Herberman'sLab,and later from ours and otherLabs have shown that activated ma-crophages from tumor bearing micehave the ability to kill tumor cells andto suppress immune response in vitro.It has been considered that these cellsmay play an important role in thehost response against tumor cellsand may also impair the host immunefunction,leading eventually to hostdeath.Thus it would be interest toinvestigate the modulation of suppres-sor macrophages by reducing theirsuppressor activity but at...

Previous works from Herberman'sLab,and later from ours and otherLabs have shown that activated ma-crophages from tumor bearing micehave the ability to kill tumor cells andto suppress immune response in vitro.It has been considered that these cellsmay play an important role in thehost response against tumor cellsand may also impair the host immunefunction,leading eventually to hostdeath.Thus it would be interest toinvestigate the modulation of suppres-sor macrophages by reducing theirsuppressor activity but at the same timeretaining their antitumor function.The ??present experiments were carried out towhether "activated" mouse peritonealmacrophages can be modulated byimmunomodulating agent,like lipo-polysaccharide(LPS),and if so,whetherthis modulation leads to any changes inthe anti-tumor function and suppressoractivity. Balb/c mouse peritoneal adherentmacrophages which were elicited byip injection 3—4 days earlier with 1 mlof 10% thioglycollatewere plated inPetri dishes(10mm)in 10 ml of 5%FCS-RPMI 1640 basic medium withor without 10 μg LPS/ml.After in-cubation at 37℃ 5% GO_2 for 18 hr,the medium was removed and washedtwice.These adherent cells weredetached in cold condition and themacrophage purity was greater than90% as assessed by morphology. MBL-2,a Moloney virus-inducedlymphoma from C57BL/6 mice wasused as the target of macrophages inthe cytostasis assay.YAC-1,a Mo-loney virus-induced lymphoma of A/Snorigin was used as the target of naturalkiller(NK)and/or natural cytotoxic(NC)cells.Both cell lines were ob-tained from Dr.R.B.Herberman'sLab(NCI,NIH,USA). Assay for suppression of NK and/orNG cells:The inhibition of NK andNG cells mediated cytotoxicity was mea-sured in a 42 hr ~(125)IUdR releaseassay in the presence and absence ofmacrophages.Assay for suppression oflymphoproliferation:Balb/c mousesplenic lymphocytes were stimulatedwith T、B lymphocyte mitogens in thepresence and absence of macrophages.After incubation for 48 hr at 37℃ 5%CO_2,each well received 0.1 μci of~(125)IUdR and microculture plates wereincubated for another 18 hr before thecells were harvested and assessed for thelevel of isotope incorporation.Assayfor cytostasis:Macrophages to be as-sayed and MBL-2 tumor cells weremixed at ratios between 20:1 and 5:1,and incubated for 48 hr in microculturewells.0.1 μci of ~(125)IUdR was addedto the cells for the last 2—4 hr of theincubation and the amount of isotopeincorporated into the tumor cells wasdetermined. The experimental results weresummarized as follows. Mouse peritoneal activative adher-ent macrophages which were elicited bythe injection of thioglycollate exhibitedstrong immune suppressor activity,asindicated by their ability to inhibitsplenic NK and/or NC cell activity andthe proliferation of splenic lymphocytesin response to the stimulation inducedby ConA,PHA,PWM and LPS.Thesesuppressor functions can be modulated(depressed)in vitro by the treatmentof these activated macrophages withhigh dose LPS(10μg/ml)(Fig.1,Table1).In contrast,the same treatment re-tained the cytostatic action of thesemacrophages against tumor cells(Table ??2.),indicating a major dissociationbetween the regulation of suppressorand cytostatic activities of macrophages.Indomethacin,an inhibitor of prostag-landin syntyetase,could not relieve orreduce the observed suppression(Table ??3 and 4).The overall result of ourpresent experiments is shown in table 5.It is suggested that in our system,macrophage population which regulatesthe immune suppressor responsivenessmay be a kind of indoimethacin-insensitive activated macrophage.Fur-ther studies to elucidate the possiblemechanism of action of such modulatedmacrophages seem warranted.

巯基乙酸盐(Thioglycollate)诱发的小鼠腹腔激活巨噬细胞显示了天然的抑制活性,即对Con A,PHA,PWM和LPS致分裂原诱导的T,B淋巴细胞的增殖能产生显著的抑制效应,同时也能抑制脾脏NK细胞对YAC-1肿瘤靶细胞的杀伤功能。但是这些巨噬细胞却同时具有明显的抗肿瘤的细胞静止效应。在体外用脂多糖(LPS,10微克/毫升)处理粘附性激活巨噬细胞明显地降低了对NK活性和T,B淋巴细胞增殖功能的抑制,然而这些免疫调变巨噬细胞仍然保持了显著的抗肿瘤的细胞静止效应。实验还表明,这类激活巨噬细胞的抑制功能不为前列腺素合成酶的抑制剂In-dom所阻断,从而提示了它们的免疫抑制效应似乎不是前列腺素介导的。本实验结果提示,体外用脂多糖处理后的免疫调变巨噬细胞是一类具有抗肿瘤功能和低免疫抑制性的巨噬细胞亚群,这为进一步研究它们的产生以及作用机理准备了条件。

A most sensitive method has been developed for the detection of Epstein-Barr ( EB ) virus in throat washings.Cryopreserved preparations of foetal cord blood lymphocytes, from samples already found to be optimally sensitive to virus-induced transformation in vitro, were exposed to concentrates prepared from throat washings by ultracentrifugation, the cells were then cultured for 8 weeks to observe the incidence of EB virus-induced transformation to lymp-hoblastoid cell lines.Using...

A most sensitive method has been developed for the detection of Epstein-Barr ( EB ) virus in throat washings.Cryopreserved preparations of foetal cord blood lymphocytes, from samples already found to be optimally sensitive to virus-induced transformation in vitro, were exposed to concentrates prepared from throat washings by ultracentrifugation, the cells were then cultured for 8 weeks to observe the incidence of EB virus-induced transformation to lymp-hoblastoid cell lines.Using this method, oropharyngeal shedding of EB virus has been monitored in a prospective study involving 30 healthy adults (all but one of Caucasian origin ) with serological evidence of previous EB virus infection.Of these individuals, 6 shed virus on every occasion of testing over a period of 15 months, 13 shed virus on most occasions, 9 shed virus on less than half the occasions of testing, whilst only 2 never gave evidence of virus. Shedding.This work indicates that the extent of EB virus shedding in healthy seropositive individuals is very much greater than what previous reports in the literature have suggested, and raises the possibility that EB virus may actually persist in vivo via chronic,usually low-grade, replication in a permissive cell type in the cropharynx.

本研究对30名血清EB病毒抗体阳性健康人从口腔排出EB病毒的情况,作了15个月的反复观察。发现28人有病毒排出,其中每次检查均为阳性者6人,间断阳性者22人,有2人多次检查均为阴性。这一结果提示,EB病毒可能是通过它在口咽部某些特定细胞内的慢性复制而在活体内持续存在的。

 
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