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clone and express     
相关语句
  克隆表达
     Objective To clone and express SARS-CoV S1 protein and to construct SARS gene vaccine.
     目的 克隆表达SARS CoVS1蛋白 ,构建SARS基因疫苗。
短句来源
     Objective To clone and express the gene encoding the binding domain of human soluble complement receptor type 1 (sCR1).
     目的克隆表达人可溶性补体受体1型(sCR1)结合C3b结构域基因。
短句来源
     Objective: To clone and express the MAGE-3 gene fragment (210~623nt) for researching its biological effects on MAGE-3 positive malignant tumors.
     目的克隆表达人MAGE-3基因片段(210~623位碱基),以便研究其对MAGE-3阳性恶性肿瘤的生物学作用。
短句来源
     Objective: To clone and express Treponema pallidum (TP) specific antigens P15 and P47 and use them in clinical examination.
     目的 :克隆表达梅毒螺旋体特异抗原 P15、P47,用于临床检测梅毒感染。
短句来源
     Objective To clone and express the gene encoding 38 kD protein of Mycobacterium tuberculosis and perform serological diagnosis using the protein as an antigen.
     目的克隆表达结核分枝杆菌38kD抗原基因,并以此蛋白为抗原,进行分枝杆菌的血清学诊断。
短句来源
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  克隆和表达
     AIM: To clone and express the gene of SLC24A6, which provide the basis to illuminate the relationship between the SLC24A6 and insulin release.
     目的:克隆和表达钾依赖钠钙交换蛋白-6(SLC24A6)基因,为进一步阐明其与胰岛素分泌的关系奠定基础。
短句来源
     Aim To clone and express the 27kDa outer membrane protein gene of Coxiella burnetii Methods com1 fragment was amplified from pGEM T/com1 by PCR,the fragment was cloned into prokaryotic expression vector pQE30;
     目的 克隆和表达贝氏柯克斯体 (Coxiellaburnetii) 2 7kDa外膜蛋白基因 (com1 )。 方法 采用PCR方法 ,从质粒pGEM -T/com1扩增出Com1基因片段 ,经鉴定后 ,将其克隆于原核表达载体 pQE30 ,构建成重组质粒 pQE30 /com1 ,IPTG诱导表达。
短句来源
     Objective:To clone and express the ns5a gene of HCV 1b DY strain and to make a base to study and utilize the gene and the expressed product.
     目的:克隆和表达丙型肝炎病毒(HCV)1b型地方株DY株ns5a基因,为进一步研究和应用该基因及其表达产物奠定基础。
短句来源
     The nucleocapsid protein (NP) is one of its main structural proteins. This study is to clone and express the HV S segment which encode the NP in Escherichia coli (Ecoli) BL21(DE3), and identify its product.
     HV核蛋白(Nucleocapsid Protein,NP)是其主要的结构蛋白之一,本文旨在对编码NP蛋白的S基因在大肠埃希菌(Escherichia coli,E.coli)BL21(DE3)中进行克隆和表达,并对表达产物进行鉴定。
短句来源
     Objective To clone and express subtype E and subtype B gag gene of the prevalent HIV 1 strain in China.
     目的 克隆和表达人免疫缺陷病毒 (HIV)Ⅰ型中国株E、B亚型代表株的结构基因gag。
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  克隆与表达
     Objective To clone and express the E2 gene in envelope region of hepatitis C virus and detect the anti E2 in sera of patients with HCV infection,using the purified E2 Protein.
     目的 对丙型肝炎病毒 (HCV)包膜区E2基因进行克隆与表达 ,并将纯化的E2蛋白用于对HCV感染者血清中E2抗体的检测。
短句来源
     Objective:To clone and express the horseradish peroxidase isozyme C3(HRPC3) gene.
     目的:克隆与表达辣根过氧化物酶同功酶C3(HRPC3)基因。
短句来源
     Objective To clone and express the pyruvate decarboxylase(PDCzm) from Zymomonas mobilis.
     目的克隆与表达移动单孢菌(Zymomonas mobilis)丙酮酸脱羧酶(Pyruvate decarboxylase,PDC)基因。
短句来源
     Objective To clone and express the fused gene fragment coding rhoptry protein ROP2 and major surface protein P30 from Toxoplasma gondii as a preparation for the construction of the complex ROP2,P30 antigen by gene engineering. Methods The gene fragment encoding P30 was amplified by PCR from T.
     目的进行弓形虫棒状体蛋白2(ROP2)和膜表面蛋白1(P30)融合基因的克隆与表达,为弓形虫ROP2-P30基因工程复合抗原的制备做准备。
短句来源
     Objective: To clone and express human and rat glial cell linederived neurotrophic factor (GDNF) cDNA.
     目的:克隆与表达人及大鼠胶质细胞源性神经营养因子(GDNF)cDNA。
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  克隆及表达
     The clone and express of NS1 of human parvovirus B19
     人细小病毒B19 NS1片段全长的克隆及表达
短句来源
     Gene clone and express of human interferon-λ 3
     人lamda3干扰素(hIFN-λ3)基因克隆及表达
     AIM: To clone and express the truncated Habb mrp gene of human Streptococcus suis type 2 (S.suis 2) and detect its activity.
     目的:克隆及表达猪链球菌2型(S.suis2)人源分离株Habb截短溶菌酶释放蛋白(MRP)基因。
短句来源
     AIM: To clone and express the testicular carcinoma antigen MAGE E1 gene in E.
     目的 :克隆及表达人胶质瘤特异性抗原MAGE E1基因片段。
短句来源
     Objective To clone and express avian influenza A virus [A/Hong Kong/482/97(H5N1)] H5 subtype hemagglutinin in baculovirus-insect cell expression system and investigate the antigenicity and bioactivity of the recombinant protein.
     目的应用杆状病毒-昆虫细胞表达体系克隆及表达禽流感病毒H5N1血凝素H5抗原,鉴定其抗原性和生物活性。
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      clone and express
    The use of phospholipases in industrial processes has grown hand-in-hand with our ability to clone and express the genes in microbial hosts with commercially attractive amounts.
          
    This host-vector system was then used successfully to clone and express a lipase gene fromArthrobacter sp.
          
    Use the following outline to clone and express your gene of interest in pcDNA3.1.
          
    This situation has been radically changed by the ability to clone and express proteins.
          


    Objective:To clone and express 6Bll ovarian ctqrcinoma anti-idioiypic antihody(Ab2β)singlechain Fv(ScFv)genes.To analyze the nucleotide sequence of ScFv.Methods:Using RT-pCR,the vari-able region genes of heavy llnd light chains(VH and VL)of 6B11 were amplified and assembled with aflexible linker sequence to form ScFv genes,which were then cloneed into,bacterial expresxion vectors toproduce proteinx,Nucleotide sequences of VH and VL were analyzed.Results:Expressed proteins fromsome clones could...

    Objective:To clone and express 6Bll ovarian ctqrcinoma anti-idioiypic antihody(Ab2β)singlechain Fv(ScFv)genes.To analyze the nucleotide sequence of ScFv.Methods:Using RT-pCR,the vari-able region genes of heavy llnd light chains(VH and VL)of 6B11 were amplified and assembled with aflexible linker sequence to form ScFv genes,which were then cloneed into,bacterial expresxion vectors toproduce proteinx,Nucleotide sequences of VH and VL were analyzed.Results:Expressed proteins fromsome clones could interact specifically with the primary anti-Ovarian carcinoma monoclonal antibody(COC166-9).The analysis of nucleotide sequence indicated that 6B11 VH and VL genes belonged to themouse Ig heavy chain subgroup I and light chain subgrnup Ⅱ,respectively.Conclusion:6B11 Ab2βSeFvgenes were successfully cloned and expressed,whch may play an imporlant role in genetically manipu-lating The murine Ah2 and make it close to clinical application for immunothcrapy of ovarian cancer.

    目的:克隆和表达6B11卵巢癌抗独特型抗体单链可变区基因,并进行序列分析。方法:采用逆转录PCR和基因重组技术,扩增并构建6B11鼠抗独特型抗体单链可变区基因,重组至噬菌体表面表达载体,在大肠杆菌表达,并利用双脱氧末端终止法进行核苷酸序列分析。结果:阳性克隆表达产物与卵巢癌单克隆抗体COC166-9特异结合;DNA序列分析表明重链和轻链可变区分属小鼠免疫球蛋白重链第I亚群和轻链第Ⅱ亚群。结论:自6B11小鼠杂交瘤细胞成功地克隆表达了该抗独特型单链抗体基因,为进一步人源化改造,推进卵巢癌的免疫治疗奠定了良好基础。

    Whole cell proteins , enriched for outer membrane antigens , of the Leptospira interrogans serogroups prevailing in China were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting ( Immunoblotting ) . Protein profiles of these serovars , representing fifteen serogroups were compared one anocher. Obvious differences were observed among these whole-cell protein profiles when the proteins of fifteen serogroups of Leptospira were probed with different antisera , includitng...

    Whole cell proteins , enriched for outer membrane antigens , of the Leptospira interrogans serogroups prevailing in China were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting ( Immunoblotting ) . Protein profiles of these serovars , representing fifteen serogroups were compared one anocher. Obvious differences were observed among these whole-cell protein profiles when the proteins of fifteen serogroups of Leptospira were probed with different antisera , includitng the differences of the number of bands and the molecular weight , Accordingly we can group the Leptospira by this method. The whole-cell protein profiles appeared extensive crossreactivity when the proteins were probed with one of these antisera ,they were composed of 3 major protein reactive bands(25 , 32 and 34 ×103) and 3-4 minor protein reactive bands. However , the obvious differences of the molecular weight of one of the major proteins ( 25 ×10~3 ) makes it possible to type the serovars. We can separate and purify the major proteins to develop subunit vaccines or clone and express the genes of these proteins to develop the genetic engineering vaccines. The results are significant to clas. and identify the Leptospira , develop the diagnostic kits and subunit vaccines and genetic engineering vaccitnes.

    分别用我国常见的15群15型钧端螺旋体多克隆抗体对我国常见的15群15型钧端螺旋体全菌蛋白进行了Western blotting分析。结果显示:用不同群的多克隆抗体与15群15型钩端螺旋体参考株的全菌蛋白反应,其图谱并不相同,表现在阳性反应带数量的多少和分子量的变化;而用同一群多克隆抗体与15群15型钩端螺旋体参考株的全菌蛋白反应,其Western blotting图谱非常相似,都有3条分子量分别为25×10~3、32×10~3和34×10~3上下的主要蛋白反应带和3~4条次要蛋白反应带,但其图谱并不完全相同,主要表现在不同群间1条主要蛋白的分子量在25×10~3上下变动,证实主要蛋白质可将不同群型钩端螺旋体进行区分;25×10~3,32×10~3和34×10~3蛋白质是所有15群15型钧端螺旋体参考株的主要外膜蛋白和抗原成分。这一研究结果对我国常见钩端螺旋体的分类和鉴定,诊断试剂盒、亚单位菌苗和基因工程菌苗的研制有重要意义。

    Objective: To clone and express green fluorescent protein S65T in E.coli. Methods: GFP S65T cDNA fragment was cloned with Nde Results: Induced by IPTG, GFP constituted more than 15% of total bacterial proteins. Induced E.coli and its ultrasonication supernatant produced strong green fluorescence when excited by long wave ultraviolet(UV) light. Conclusion: GFP S65T can be used as a report gene in prokaryotes, and its expression can be easily detected by long wave UV.

    目的:在大肠杆菌中克隆及高效表达绿色荧光蛋白基因(GFP-S65T)。方法:PCR法克隆GFP-S65TcDNA并改造其两端的限制性内切酶位点,构建重组原核表达载体pRSET-GFPS65T。结果:在IPTG诱导条件下,GFP-S65T的表达量占细菌总蛋白量的15%以上。细菌培养物及其超声上清在长波紫外线的照射下,发出明亮的绿色荧光。结论:GFP-S65T可以作为原核细胞中的报告基因,用标准的长波紫外线即可检测其表达。

     
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