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clone and express    
相关语句
  克隆和表达
    Objective:To clone and express the ns5a gene of HCV 1b DY strain and to make a base to study and utilize the gene and the expressed product.
    目的:克隆和表达丙型肝炎病毒(HCV)1b型地方株DY株ns5a基因,为进一步研究和应用该基因及其表达产物奠定基础。
短句来源
    Gene clone and express of human interferon-ε
    人Epsilon干扰素(hIFN-ε)的基因克隆和表达
    The nucleocapsid protein (NP) is one of its main structural proteins. This study is to clone and express the HV S segment which encode the NP in Escherichia coli (Ecoli) BL21(DE3), and identify its product.
    HV核蛋白(Nucleocapsid Protein,NP)是其主要的结构蛋白之一,本文旨在对编码NP蛋白的S基因在大肠埃希菌(Escherichia coli,E.coli)BL21(DE3)中进行克隆和表达,并对表达产物进行鉴定。
短句来源
    Objective To clone and express subtype E and subtype B gag gene of the prevalent HIV 1 strain in China.
    目的 克隆和表达人免疫缺陷病毒 (HIV)Ⅰ型中国株E、B亚型代表株的结构基因gag。
短句来源
    Objective To clone and express the structural gene encoding a 31 kDa antigen of Trichinella spiralis (Henan isolate) pre encysted larvae (TspE1).
    目的 克隆和表达旋毛虫河南地理株成囊前期幼虫编码 3 1kDa抗原的结构基因 (TspE1)。
短句来源
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  克隆表达
    Objective: To clone and express human monocyte chemoattractant protein-1 (huMCP-1 ) with important biologic functions.
    目的:克隆表达具有重要生物功能的人单核细胞趋化蛋白1(huMCP-1)。
短句来源
    Objective To clone and express SARS-CoV S1 protein and to construct SARS gene vaccine.
    目的 克隆表达SARS CoVS1蛋白 ,构建SARS基因疫苗。
短句来源
    AIM:To clone and express SARS CoV S protein and to construct SARS gene vaccine.
    目的:克隆表达SARS-CoVS蛋白,构建SARS全长基因疫苗。
短句来源
    Objective To clone and express the gene encoding the binding domain of human soluble complement receptor type 1 (sCR1).
    目的克隆表达人可溶性补体受体1型(sCR1)结合C3b结构域基因。
短句来源
    AIM: To clone and express the Mr 16×103 and Mr 38×103 proteins of Mycobacterium tuberculosis in E. coli, and to characterize its antigenicity and specificity.
    目的:克隆表达结核分枝杆菌Mr16×103和Mr38×103重组蛋白,测定其抗原性和特异性.
短句来源
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  克隆与表达
    Objective: To clone and express human and rat glial cell linederived neurotrophic factor (GDNF) cDNA.
    目的:克隆与表达人及大鼠胶质细胞源性神经营养因子(GDNF)cDNA。
短句来源
    Objective To clone and express the E2 gene in envelope region of hepatitis C virus and detect the anti E2 in sera of patients with HCV infection,using the purified E2 Protein.
    目的 对丙型肝炎病毒 (HCV)包膜区E2基因进行克隆与表达 ,并将纯化的E2蛋白用于对HCV感染者血清中E2抗体的检测。
短句来源
    Objective:To clone and express the gene of α-toxin from Clostridium perfringens(CPa).
    目的 :实现产气荚膜梭菌α毒素 (Clostridiumperfringensalphatoxin ,CPa)基因的克隆与表达
短句来源
    Objective To clone and express the fused gene fragment coding rhoptry protein ROP2 and major surface protein P30 from Toxoplasma gondii as a preparation for the construction of the complex ROP2,P30 antigen by gene engineering. Methods The gene fragment encoding P30 was amplified by PCR from T.
    目的进行弓形虫棒状体蛋白2(ROP2)和膜表面蛋白1(P30)融合基因的克隆与表达,为弓形虫ROP2-P30基因工程复合抗原的制备做准备。
短句来源
    Study on clone and express endothelial nitric oxide synthase traffic inducer protein
    内皮型一氧化氮合酶运输诱导物NOSTRIN基因的克隆与表达
短句来源
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  克隆表达
    Objective: To clone and express human monocyte chemoattractant protein-1 (huMCP-1 ) with important biologic functions.
    目的:克隆表达具有重要生物功能的人单核细胞趋化蛋白1(huMCP-1)。
短句来源
    Objective To clone and express SARS-CoV S1 protein and to construct SARS gene vaccine.
    目的 克隆表达SARS CoVS1蛋白 ,构建SARS基因疫苗。
短句来源
    AIM:To clone and express SARS CoV S protein and to construct SARS gene vaccine.
    目的:克隆表达SARS-CoVS蛋白,构建SARS全长基因疫苗。
短句来源
    Objective To clone and express the gene encoding the binding domain of human soluble complement receptor type 1 (sCR1).
    目的克隆表达人可溶性补体受体1型(sCR1)结合C3b结构域基因。
短句来源
    AIM: To clone and express the Mr 16×103 and Mr 38×103 proteins of Mycobacterium tuberculosis in E. coli, and to characterize its antigenicity and specificity.
    目的:克隆表达结核分枝杆菌Mr16×103和Mr38×103重组蛋白,测定其抗原性和特异性.
短句来源
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  clone and express
The use of phospholipases in industrial processes has grown hand-in-hand with our ability to clone and express the genes in microbial hosts with commercially attractive amounts.
      
This host-vector system was then used successfully to clone and express a lipase gene fromArthrobacter sp.
      
Use the following outline to clone and express your gene of interest in pcDNA3.1.
      
This situation has been radically changed by the ability to clone and express proteins.
      


Whole cell proteins , enriched for outer membrane antigens , of the Leptospira interrogans serogroups prevailing in China were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting ( Immunoblotting ) . Protein profiles of these serovars , representing fifteen serogroups were compared one anocher. Obvious differences were observed among these whole-cell protein profiles when the proteins of fifteen serogroups of Leptospira were probed with different antisera , includitng...

Whole cell proteins , enriched for outer membrane antigens , of the Leptospira interrogans serogroups prevailing in China were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting ( Immunoblotting ) . Protein profiles of these serovars , representing fifteen serogroups were compared one anocher. Obvious differences were observed among these whole-cell protein profiles when the proteins of fifteen serogroups of Leptospira were probed with different antisera , includitng the differences of the number of bands and the molecular weight , Accordingly we can group the Leptospira by this method. The whole-cell protein profiles appeared extensive crossreactivity when the proteins were probed with one of these antisera ,they were composed of 3 major protein reactive bands(25 , 32 and 34 ×103) and 3-4 minor protein reactive bands. However , the obvious differences of the molecular weight of one of the major proteins ( 25 ×10~3 ) makes it possible to type the serovars. We can separate and purify the major proteins to develop subunit vaccines or clone and express the genes of these proteins to develop the genetic engineering vaccines. The results are significant to clas. and identify the Leptospira , develop the diagnostic kits and subunit vaccines and genetic engineering vaccitnes.

分别用我国常见的15群15型钧端螺旋体多克隆抗体对我国常见的15群15型钧端螺旋体全菌蛋白进行了Western blotting分析。结果显示:用不同群的多克隆抗体与15群15型钩端螺旋体参考株的全菌蛋白反应,其图谱并不相同,表现在阳性反应带数量的多少和分子量的变化;而用同一群多克隆抗体与15群15型钩端螺旋体参考株的全菌蛋白反应,其Western blotting图谱非常相似,都有3条分子量分别为25×10~3、32×10~3和34×10~3上下的主要蛋白反应带和3~4条次要蛋白反应带,但其图谱并不完全相同,主要表现在不同群间1条主要蛋白的分子量在25×10~3上下变动,证实主要蛋白质可将不同群型钩端螺旋体进行区分;25×10~3,32×10~3和34×10~3蛋白质是所有15群15型钧端螺旋体参考株的主要外膜蛋白和抗原成分。这一研究结果对我国常见钩端螺旋体的分类和鉴定,诊断试剂盒、亚单位菌苗和基因工程菌苗的研制有重要意义。

Objective: To clone and express green fluorescent protein S65T in E.coli. Methods: GFP S65T cDNA fragment was cloned with Nde Results: Induced by IPTG, GFP constituted more than 15% of total bacterial proteins. Induced E.coli and its ultrasonication supernatant produced strong green fluorescence when excited by long wave ultraviolet(UV) light. Conclusion: GFP S65T can be used as a report gene in prokaryotes, and its expression can be easily detected by long wave UV.

目的:在大肠杆菌中克隆及高效表达绿色荧光蛋白基因(GFP-S65T)。方法:PCR法克隆GFP-S65TcDNA并改造其两端的限制性内切酶位点,构建重组原核表达载体pRSET-GFPS65T。结果:在IPTG诱导条件下,GFP-S65T的表达量占细菌总蛋白量的15%以上。细菌培养物及其超声上清在长波紫外线的照射下,发出明亮的绿色荧光。结论:GFP-S65T可以作为原核细胞中的报告基因,用标准的长波紫外线即可检测其表达。

Objective: To clone and express human and rat glial cell linederived neurotrophic factor (GDNF) cDNA. Methods and Results: The cDNA encoding human and rat GDNF was amplified by RTPCR. Then the human and rat GDNF cDNA was cloned into the expression vector pBPL. When transfected into E.coli, the recombinant plasmid pBPL G expressed a 16 000 protein, matching with its deduced molecular mass. Conclusion: Successful cloning and expression of human and rat GDNF lay foundations for studying the role...

Objective: To clone and express human and rat glial cell linederived neurotrophic factor (GDNF) cDNA. Methods and Results: The cDNA encoding human and rat GDNF was amplified by RTPCR. Then the human and rat GDNF cDNA was cloned into the expression vector pBPL. When transfected into E.coli, the recombinant plasmid pBPL G expressed a 16 000 protein, matching with its deduced molecular mass. Conclusion: Successful cloning and expression of human and rat GDNF lay foundations for studying the role of GDNF in the lesion and repairing of nervous system.

目的:克隆与表达人及大鼠胶质细胞源性神经营养因子(GDNF)cDNA。方法和结果:用逆转录-聚合酶链反应(RT-PCR)扩增了人及大鼠GDNF成熟序列的cDNA片段,并将人及大鼠GDNFcDNA重组到表达质粒pBPL中,分别在大肠杆菌中获得了较高表达。结论:人及大鼠GDNFcDNA的克隆与表达获得成功,为研究GDNF在神经损伤修复中的作用奠定了基础。

 
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