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viral progenies
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  子代病毒
     Barstar was a specific inhibitor of barnase. Recombinant virus rHa Bar were infected into the AM1 NB and normal cells HZ AM1 respectively. After 48 hours and 72 hours viral progenies in AM1 NB were 23 times and 160 times as many as that in HZ AM1 cells respectively.
     用 barnase的拮抗基因 barstar构建了具有 Neo抗性、并能稳定表达 barstar的棉铃虫转化细胞 AM1 - NB.以携带 barnase基因的重组病毒 r Ha- Bar分别感染转化细胞和正常细胞 ,48h子代病毒在转化细胞中的产量比在正常细胞中高 2 3倍 ,72 h高 1 60倍 .
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  相似匹配句对
     and G. thurherii D. progenies.
     x瑟伯氏棉(G.thurberii D.)
短句来源
     Viral Hepatitis and Typhoid
     肝炎和伤寒流行过程的混沌分维研究
短句来源
     the viral genome was stable;
     病毒基因组稳定;
短句来源
     Two reassortants of FH4232 and SA11 were obtained from the purified progenies of the 13th passage of mixed viral strain.
     重组子代病毒株的第5、11条电泳带来自亲代FH4232株,其余多条则来自亲代SA11株。
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     Selection For Brix in Sugarcane Hybrid Progenies
     甘蔗杂交后代锤度的选择
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A recombination shuttle vector (Hanpvid) pHa Bar expressing barnase gene ( bar ) under control by polyhedrin gene ( ph ) promoter was constructed after transposition by transfoming recombination plasmid pFb Bar carrying ph and barnase gene ( bar ) into E.coli DH15Ha,which contained transposon shuttle vector Hanpvid comprising of Heliothis armigera nuclear polyhedrosis virus (HaNPV) genomic DNA and a transposon cassette.Recombinant HaNPV(rHa Bar) was obtained by transfected recombinant Hanpvid...

A recombination shuttle vector (Hanpvid) pHa Bar expressing barnase gene ( bar ) under control by polyhedrin gene ( ph ) promoter was constructed after transposition by transfoming recombination plasmid pFb Bar carrying ph and barnase gene ( bar ) into E.coli DH15Ha,which contained transposon shuttle vector Hanpvid comprising of Heliothis armigera nuclear polyhedrosis virus (HaNPV) genomic DNA and a transposon cassette.Recombinant HaNPV(rHa Bar) was obtained by transfected recombinant Hanpvid into Heliothis armigera cells HZ AM1.Dot blotting analysis showed that bar gene transcript RNA could be detected,therefore,both bar and ph gene were expressed in HZ AM1 cells.Black cycle could be seen on the plate containing yeast tRNA( 0.5% ) as a substrate for RNA digested by barnase.The three instar Heliothis armigera larvare were used for biological assay.Results showed that lethal doses ( LD 50 ) were reduced by 20% and lethal time ( LT 50 ) were reduced by 30% compared with wild type HaNPV.A clonal transformed cell line AM1 NB,in which barstar gene ( bst ) and neomycin gene were integrated into cell chromosome and stably expressed,was isolated.Barstar was a specific inhibitor of barnase.Recombinant virus rHa Bar were infected into the AM1 NB and normal cells HZ AM1 respectively.After 48 hours and 72 hours viral progenies in AM1 NB were 23 times and 160 times as many as that in HZ AM1 cells respectively.

将含有 barnase基因与杆状病毒多角体基因 ( ph)的重组转座载体 p Fb- Bar在大肠杆菌中与含有棉铃虫核型多角体病毒 ( Ha NPV)的穿梭载体 Hanpvid转座并提取重组穿梭载体 DNA转染棉铃虫细胞 ,得到重组棉铃虫病毒 r Ha- Bar.其分子杂交证明 ,昆虫细胞中有 r Ha- Bar的 bar基因转录本存在 ,并能表达产生 33k D的多角体蛋白和 1 2 k D的 barnase.在平板上 ,barnase能降解RNA,出现清晰的降解圈 .r Ha- Bar对三龄棉铃虫幼虫的毒力比野生型 Ha NPV的 LD50 减少 2 0 % ,LT50 减少 30 % .用 barnase的拮抗基因 barstar构建了具有 Neo抗性、并能稳定表达 barstar的棉铃虫转化细胞 AM1 - NB.以携带 barnase基因的重组病毒 r Ha- Bar分别感染转化细胞和正常细胞 ,48h子代病毒在转化细胞中的产量比在正常细胞中高 2 3倍 ,72 h高 1 60倍 .

 
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