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seeded
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  种子
    Experimental Study on EPCs and MSCs as Seeded Cells of Tissue Engineering Heart Valve
    EPCs联合MSCs作为组织工程心脏瓣膜种子细胞实验研究
短句来源
    Establish a Fluid Flow Shear Stress Model for Culturing Bone Engineering Seeded Cells in Vitro
    一种骨种子细胞体外应力培养系统的建立
短句来源
    Experimental Study of Applying Bone Marrow Mesenchymal Stem Cells as Tissue Engineering Valve Seeded Cells
    应用骨髓间充质干细胞为种子细胞构建组织工程瓣膜的实验研究
短句来源
    The principle of the construction of tissue engineering SMG is that the SMG cells are isolated and cultured in vitro, and the seeded cells in the three - dimensional scaffolds.
    因此,种子细胞的培养,可供种子细胞进行生命活动的支架材料筛选与开发,及种子细胞与支架材料相互作用的研究成为组织工程学研究的核心问题。
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    Co-culture accellular umbilical veins with seeded cells .
    研究脱细胞人脐静脉基质与种子细胞的共培养。
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    The second generation cells were seeded on eggshell membrane, and then induced with osteogenic media(50 μg/ml ascorbic acid, 10 mmol/L β-glycerophosphate, and 0.1 μmol/L dexamethasone) for 2 weeks in vitro. The cell-seeded scaffolds were implanted subcutaneously in Wistar rats for 4 weeks.
    将第2代BMSCs同鸡蛋膜支架材料复合,利用含0.1μmol/L地塞米松、10mmol/Lβ-甘油酸钠、50μg/ml维生素C的条件培养液诱导培养2周后,分别将复合材料植入Wistar大鼠右侧背部皮下,左侧植入无细胞复合的鸡蛋膜作为对照。
短句来源
    Methods: The rabbit bone marrow stromal cells (BMSCs) of generation 2 were seeded onto the biomaterials (RGD-PLGA/HA and PLGA/HA) and incubated for 4? 12 and 24 hours at standard condition. At each time point the number of adhesive cells was counted.
    方法:以RGD-PLGA/HA膜和作为对照的未结合RGD肽的PLGA/HA材料为实验对象,取第二代兔骨髓基质细胞(bone marrow stromal cells,BMSCs)接种到材料上,培养4、12、24h后,细胞计数仪计数定量检测粘附的细胞数,得到粘附率;
短句来源
    Results The porous structure of the scaffold facilitated the penetration and attachment of the seeded cells. The porosity was 83.7% and the pore size was 100-120 μm.
    结果胶原-透明质酸支架材料经化学交联后,具有合适的三维多孔结构,孔隙率为83.7%,孔径100~120μm;
短句来源
    RESULTS: ①Isolation, amplification and identification of epidermal stem cells: Before the amplified cells seeded on the acellular epidermis, keratin 19 (K19) was identified with immunohistochemistry, and α6 and CD71 with flow cytometry.
    结果:①表皮干细胞的分离、扩增及鉴定:在扩增的细胞拟接种于无细胞真皮前,进行角蛋白19免疫组织化学鉴定、以流式细胞术进行α6、CD71鉴定。
短句来源
    Methods The keratinocytes of rat were seeded with the BSA-CGG,CGG,CG respectively, the cytotoxicity was detected by MTT.
    [方法]应用MTT比色法,测定BSA-CGG,CGG,CG三种材料对大鼠原代表皮细胞的细胞毒性和细胞增殖率;
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  seeded
A systematic investigation was performed on the influence of several factors such as ageing of the reaction mixture, alkalinity, salt addition and temperature on the formation of a mordenite membrane on the seeded support.
      
Polymer beads with a diameter of 60-70 nm and specific functional groups were synthesized by a particular seeded emulsion polymerization technique.
      
Bone mesenchymal stem cells were seeded in the SIS, and the scaffold-cell constructs were cultured in vitro for 2 weeks.
      
The growth of silicalite-1 membranes on different seeded supports by hydrothermal synthesis was also evaluated.
      
With this method of seeded secondary synthesis of zeolite membranes, the quality of a membrane mainly depends on the quality of the seed layer.
      
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The present study aimed to clarify the promising inhibiting effect of L-Glutamic acid on GA or GA+EC preserved porcine valves, and illustrate the processes of the leaflets surface covered by SD sucking rat's aortic endothelial cells. The porcine aortic valves were treated in following 4 groups: 1 ) 0. 5% GA treated valves, 2) none special biochemical treated valves, 3) valves treated by 0. 5% GA and then in 8% L-Glutamic acid solution, 4) 0. 5% GA for 72 hrs then 4% EC for 48 hrs before stored in the solution...

The present study aimed to clarify the promising inhibiting effect of L-Glutamic acid on GA or GA+EC preserved porcine valves, and illustrate the processes of the leaflets surface covered by SD sucking rat's aortic endothelial cells. The porcine aortic valves were treated in following 4 groups: 1 ) 0. 5% GA treated valves, 2) none special biochemical treated valves, 3) valves treated by 0. 5% GA and then in 8% L-Glutamic acid solution, 4) 0. 5% GA for 72 hrs then 4% EC for 48 hrs before stored in the solution of 8% L- Glutamic acid. Counting seeded-cells from valve leaflets; Identification of cellular type by factor Ⅷ rAG; and electronmicroscopic assessment after endothelial cells were seeded in 1, 3, 7, and 14 days. The result showed: All the leaflets of group 2, 3, and 4 could acquire the endothelial cells and fibroblasts covering during 7-14 days. The covering rate in group 2 exhibited more than 80% after 7 days but in group 1 no viable cells could be seen in 2-3 days after implanting. The study revealed that the toxicity of GA in the heterografts were inhibited markedly by L-Glutamic acid and their biocompatibility were improved that make sucking rat aortic endothelium could grow regularly on the surface of bioprosthetic valves.

我们将经过戊二醛(GA)和GA+环氧氯丙烷(EC)化学改性处理的猪主动脉瓣,再用L—谷胺酸处理以后进行SD乳鼠主动脉内皮细胞种植,并与单纯GA瓣及未经GA处理的瓣叶做对比观察。结果发现:单纯GA瓣种植细胞未能存活,而经L—谷胺酸处理瓣叶却获得种植细胞规律性生长、增殖,两周后完全覆盖瓣叶表面。电镜观察及第Ⅷ因子染色证实,瓣小叶表面生长的内皮细胞中夹杂着大量成纤维细胞生长。与种植前培育细胞纯化及种植以后成纤维细胞控制不够有关。

The isolated mice peritoneal macrophages in degradation of calcium phosphate compound artificial bone - collagen/hydroxylapatite (CHA). hydroxylapatite (HA), beta-tricalcium phosphate (TCP) ceramics, have been studied by use of both Ca++, P concentration assay in cultured supernatant and scanning electron microscopy (SEM). The solubility of Ca++ . composition of materials increased more significantly when macrophages were inoculated than when macrophages were not seeded (P< 0.001), and it was shown that...

The isolated mice peritoneal macrophages in degradation of calcium phosphate compound artificial bone - collagen/hydroxylapatite (CHA). hydroxylapatite (HA), beta-tricalcium phosphate (TCP) ceramics, have been studied by use of both Ca++, P concentration assay in cultured supernatant and scanning electron microscopy (SEM). The solubility of Ca++ . composition of materials increased more significantly when macrophages were inoculated than when macrophages were not seeded (P< 0.001), and it was shown that the ground materials were wrapped and phagocytized or resorbed extracellularly by macrophages under SEM, suggesting that macrophages could mediate the degradation of calcium phosphate compound artificial bone by phagocytizing and/or degrading extracellularly.

(夏志道)(朱通伯)(杜靖远)(郑启新)(汪岚)MacrophagesinDegradationofCalciumPhosphateCompoundArtificialBone:AninvitroStudy¥XIAZhi-dao;ZHUTong-bo;DUJi...

e used Colla Corii Asini(CCA)to precoat vascul ar prostheses, then the grafts were seeded withEndothelial cells(EC).The results showed:Colla Corii Asini could improve the adhesion rate of ECon the grafts significantly. The group with concentration of 10%had the best results,and had satisfac-tory effects on the growth and morphology of EC. As Compared with other precoating substances,theeffects of CCA were analogous to fibronectin,and suprior to geltin and albumin. The major componentsof CCA are nonglucoprotein...

e used Colla Corii Asini(CCA)to precoat vascul ar prostheses, then the grafts were seeded withEndothelial cells(EC).The results showed:Colla Corii Asini could improve the adhesion rate of ECon the grafts significantly. The group with concentration of 10%had the best results,and had satisfac-tory effects on the growth and morphology of EC. As Compared with other precoating substances,theeffects of CCA were analogous to fibronectin,and suprior to geltin and albumin. The major componentsof CCA are nonglucoprotein which can inhibit the adhesion of platelet,thus reduce thrombosis.

用中药阿胶预衬人工血管后种植内皮细胞(EC)。结果显示:阿胶能明显提高EC在人工血管的粘附率,其中以10%浓度阿胶效果最好,而且对EC的生长和形态均有良好影响。阿胶的作用与纤维结合素相近,优于明胶和白蛋白。主要成份蛋白质多为非糖链蛋白,可能抑制血小板在人工血管的粘附而减少血栓形成的机会

 
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