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sterile     
相关语句
  不育
     Identifying on Photo-thermo Sensitive Genic Male Sterile Lines in Rice and Study on Xa21 Transgenic Progeny of Peiai64S
     水稻光温敏核不育系鉴定及培矮64S转Xa21基因后代分析
短句来源
     Fertility Expression and Stability of the Photo- and Thermo-sensitive Genic Male Sterile Rice Pei'ai 64S under Controlled Low Temperature Conditions
     光温敏核不育水稻培矮64S低温下育性表达及其稳定性研究
短句来源
     Basic Studies on the Inverse Temperature-sensitive Dual-purpose Genic Male Sterile Line Go543s
     水稻反温敏两用核不育系go543s的应用基础研究
短句来源
     Study on Molecular Cytogenetics of Nian Type CMS and Development of Its the New Sterile System by Substituting Fertility Gene Carrier in Wheat
     粘类小麦CMS分子细胞遗传学研究及其育性基因载体替换后新不育体系的建拓
短句来源
     Studies on Genetic Mechanism of Cytoplasmic Male Sterile Pepper and the Molecular Markers Related to the CMS Gene
     辣(甜)椒细胞质雄性不育系遗传机制及不育基因分子标记研究
短句来源
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  无菌
     However, the sterile laminas and leafstalks of regenerative plantlets can be used for inducing callus, and the optimum medium are WPM+2.0mg/LBA+0.5mg/L2,4-D and WPM+2.0mg/LBA+2.0mg/LNAA, respectively.
     以珙桐无菌芽叶片和叶柄诱导愈伤组织则获得成功,适宜的培养基分别为WPM+2.0mg/LBA+0.5mg/L2,4-D和WPM+2.0mg/LBA+2.0mg/LNAA。
短句来源
     A new sterile ~(113m)In generator
     新型无菌~(113m)In发生器
短句来源
     The eligible rates of indoor air,surfaces,disinfection solution in use,sterile instruments and autoclave were 52.94%,86.3%,(94.57%),81.88% and 76.92% respectively.
     室内空气合格率为52.94%,物体表面合格率为86.3%,使用中消毒液合格率为94.57%。 无菌器械和压力蒸汽灭菌器检测合格率分别为81.88%和76.92%。
短句来源
     The quality control of sterile ~(99m)Tc and ~(115m)In generators
     无菌~(99m)Tc、~(113m)In发生器的质量检验
短句来源
     I/R group received the equivalent volume((0.5) ml) of sterile PBS.
     I/R组供体给予等体积0.5 ml无菌PBS液。
短句来源
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  不育的
     Their hybrid F_1was sterile.
     其杂种F_1是不育的
短句来源
     W6154s was sterile at above 27.12 degree centigrade, and fertile below this temperature.
     W6154s育性在较高温度(>27.12℃)下表现不育,在较低温度下表现可育,诱导完全不育的临界温度在27.12℃以上。
短句来源
     Among the 58 diploid somaclones, 20 were sterile, making up 34-5%.
     58个二倍体无性系申不育的为20个,占34.5%。
短句来源
     Preliminary study on inheritance of Brassica juncea thermosensitive genicmale sterile line 'K121S'
     油菜K121S温敏核不育的遗传初探
短句来源
     1. Pollen abortion is an indicator for identifying the male sterile plants. Pollen fertilityof F1 plants from five different crosses, including 167×YB, YA×167, YA×Y12R, ZD8319×ZB, and ZA×ZD8319 were examined. All of the F1 pollen is semi-sterile, which providedevidence that the traits of male sterility in these lines are controlled by a single-genicgametophytic-type system.
     1.以花粉败育率作为衡量大豆细胞质雄性不育植株育性的指标,组配了(167×YB)、(YA×167)、(YA×Y12R)、(ZD8319×ZB)和(ZA×ZD8319)五个杂交组合,通过 F1 育性检查,所有 F1 花粉均表现为半不育,这一现象是单基因配子体不育的典型特征。
短句来源
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  无菌的
     Among the 48 VAP patients,there were 64.6%(31/48) of them with positive pathogen obtained before tracheotomy,whereas only 35.4%(17/48) with sterile tracheal aspirate culture(P<0.01).
     48例VAP患者,气管插管前气管吸出物中即有病原菌存在的占64.6%(31/48),显著高于插管前气管吸出物无菌的患者(35.4%,17/48)(P<0.01)。
短句来源
     Methods After emerging adult mosquitoes were fed for 3 consecutive days on antibiotic solution, midguts dissected under sterile condition were kept in Grace's medium containing 7% fetal bovine serum, 0.5% gentamycin, 1 mg/ml yeastolate and 6 μl/ml vitamin premix to incubate at 25℃.
     方法 用含抗生素的 10 %蔗糖水喂养新羽化出的埃及伊蚊成蚊 ,3d后 ,在无菌的条件下解剖中肠 ,用含抗生素的平衡盐溶液洗 3次 ,放入Grace’s培养基中 (含有 7%小牛血清 ,0 .5 %庆大霉素 ,1mg/ml抗酵母菌素和 6μl/ml复合维生素 ) ,2 5℃培养箱中培养。
短句来源
     After cut to 1 mm thin layer and immersed in 0.5% sterile citric acid for few minutes, the stems of the tube seedling of Huangdi banana( Musa paradisica AA) in vitro were cultured in different media for induction of callus and organogenesis.
     将皇帝蕉试管苗茎段徒手切成厚约1mm的薄片,经无菌的0.5%柠檬酸溶液处理片刻后,接入各种培养基中。
短句来源
     INTERVENTIONS:Five days after chronic constrictive injury(CCI) model was estab lished,different doses of U0126 were intrathecally injected according to Mestre' s method. The control group received sterile intrathecal injection of 50 g/L dime thylsulfoxide.
     干预:慢性压迫性损伤(chronicconstrictioninjury,CCI)模型建立后第5天,采用Mestre直接鞘内注射方法行不同浓度的U0126注射,同时在对照组给予鞘内注射无菌的50g/L二甲基亚砜。
短句来源
     Mice bone marrow is obtained under sterile condition,then mononuclear cells were isolated by density gradient centrifugation. The cells are divided into three groups,which is cultured in different media contained diverse ECGS concentration respectively:A groupxontain ECGS 300ug/ml; B groupxontain ECGS 200ug/ml;
     在无菌的条件下抽取大鼠骨髓,用密度梯度离心法分离骨髓单核细胞,将细胞分为三组,接种在添加不同浓度ECGS的基础内皮培养基中:A组:含ECGS 300ug/ml:B组:含ECGS 200 ug/ml;
短句来源
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      sterile
    Fine mapping of an Arabidopsis thaliana male sterile mutant EC2-157
          
    An Arabidopsis thaliana male sterile mutant EC2-157 has been isolated using an EMS mutagenesis strategy.
          
    As no male sterile genes have been reported in this region, ms157 could be a novel gene related to fertility.
          
    Physicochemical Properties of Melanins Produced by the Sterile Form of Inonotus obliquus("Chagi") in Natural and Cultivated Fung
          
    Physicochemical properties of pigments isolated from the naturally occurring sterile form of Inonotus obliquus(Fr.) Pil.
          
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    The present report deals with the results of some studies on the longevity of the soft-rot organism, Erwinia aroideae (Towns.) Holland in soil. It is found that when the bacteria are added into a mass of non-sterile field soil in a test-tube and incubated at 28℃, they can not live longer than 14 days. This result is somewhat contradictory to those of Patel, but agrees with those of Gorlenko and Voronkeviz. A study of the number of microorganisms which, are able to be plated out in beef bouillon peptone...

    The present report deals with the results of some studies on the longevity of the soft-rot organism, Erwinia aroideae (Towns.) Holland in soil. It is found that when the bacteria are added into a mass of non-sterile field soil in a test-tube and incubated at 28℃, they can not live longer than 14 days. This result is somewhat contradictory to those of Patel, but agrees with those of Gorlenko and Voronkeviz. A study of the number of microorganisms which, are able to be plated out in beef bouillon peptone agar reveals that when a known number of soft-rot bacteria is added into a soil sample, the total number of the indigenous microorganisms is increased, while the number of the introduced bacteria is rapidly decreased. After 72 hours, the total number of the indigenous microorganisms in the soil sample begins to decrease and finally reaches its original level. It is inferred that following the addition of the soft-rot organism into a soil sample, a biological equilibrium is broken and the indigenous microorganisms are stimulated to multiply rapidly. After a brief period of such an unusual multiplication of the indigenous microorganisms, the invaders are suppressed and a new equilibrium is finally established. Henceforth the number level of the indigenous microorganisms in the soil sample returns to the orginal. When the soft-rot bacteria are introduced into the soil in soil tanks regulated at different soil temperatures (and in each tank two plants of the Chinese cabbage are planted), the introduced bacteria live 11 days at 35℃, 21 days at 10℃ and 7 days at 20℃. Possibly a soil temperature at 20℃ might be close to the optimal for the activities of some antagonists of the soft-rot bacteria. When a soil sample is fertilized with the "de-oiled soybean-seed-cake" or with the "dried night soil", the elimination of the intruding bacteria is accelerated. In the plot where the de-oiled soybeanseed-cake" is applied, the soft-rot organism disappears within 24 hours, whereas in the plot of the "dried night soil", the intruding bacteria disappear within 5 days. The soft-rot bacteria in an autoclaved field soil sample live 96 days. When a quantity of manure or compost is added to the soil sample and chert autoclaved', the soft-rot bacteria introduced at such a condition live longer than 180 days. Evidently the added manure or compost in sterile condition serves as food material for the soft-rot organism.

    白菜軟腐細菌(Erwinia aroideae)在不灭菌的土壤中可以存活11天至14天。土壤中的微生物总数(指可以在牛肉汁(月柬)培养基上发育的微生物)因軟腐細菌的加入而起剧烈的波动。当軟腐細菌进入土壤中后24小时,軟腐細菌的数量銳减而土壤中的其他微生物数量上昇,以后亦逐漸递減。当軟腐細菌在土壤中消失时,土壤微生物的数量又趋于平稳。軟腐細菌在栽有白菜的土壤中,土壤温度影响其存活期:土温在35℃时可以存活11天,在10℃可以存活21天,在20℃时可以活7天。豆餅及大粪干施入土壤中时可以促进軟腐細菌的消灭。施豆餅区(豆餅微粒与土壤拌匀)在24小时中软腐細菌即已消灭,而在施大粪干区軟腐細菌可以存活5天。軟腐細菌在灭菌的土壤中可以活96天,如果施以灭菌的堆肥或厩肥时,可以延长至180天以上。

    Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different...

    Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different culture media such as PDA, acidified PDA, cotton meal agar and rice agar. All these plates failed to show verticillium colonies. Among the fungi obtained in these plates, Fusarium, Alternaria and Colletotrichum were the most frequent. Since both Verticillium albo-atrum and other fungi were harbored within the same seed, the difference of growth rates might be considered as an important factor involved in the difficulty of the isolations: Cultures of V. albo-atrum and Fusarium sp. were transferred simutaneously to PDA plates to test their growth rates. The diameter of their colonies were measured after incubation for 12 days under 22±1℃. Those of V. albo-atrum measured 2.8 cm., while the average of the latter was 9,3 cm. Interference of the fast-growers might render a slow-grower such as V. albo-atrum to be masked in the observations and to be depressed in the isolations. A method was developed to avoid the interference. The fundamental procedures of the method are outlined as follows. 1. Plating and observation. (1) Cotton seeds being delinted with commercial surphuric acid in order to avoid the interference from lint-born contaminants. (2) Delinted seeds being washed in a flask covered with wire gauze for 24 hours under running tap water in order to get rid of bacteria. Absorption of sufficient water during washing activates the hibernating mycelium. (3) Seeds being plated on 1.7% water agar instead of nutrient agar in order to diminish the growth of the fast-growers such as Fusaria and Alternaria. The plates being incubated under 22±1℃. for 15 days. (4) The plates being uncovered and observed directly under low-power microscope in order to observe the fungi undisturbed. 2. Isolation and preservation of the Verticillium cultures. (1) Verticilliate conidiophores being located under low-power microscope. (2) The tip of a flamed needle moistened with sterile agar being introduced within the microscopic field in order to fish the spores under focus. (3) Spore suspension being made by washing the needle in a 5 cc sterile water blank. (4) Dilution plates being made by placing 1 cc of the spore suspension in a petri dish and diluting with 10 cc of cotton stem agar. (5) The plates being observed under low-power microscope after incubation for 5—7 days at 22±1℃. (6) Verticillium colonies being locatad and a bit of the margin of a colony being transferred to PDA slant. (7) The slant cultures being flooded with sterile liquid paraffin after incubation for 7—15 days at 22±1℃. The cultures preserved with liquid paraffin were able to keep alived for 2 years. Seeds collected from the infected plants in 1955, 1956 and 1957 were studied. The percentages of seeds carrying Verticillium observed in these years were 39.8, 5.92 and 9.5% respectively. One of the representative single spore colonies obtained was identified as V. albo—atrum. Inoculation and reisolation tests showed positive results. Inspection of cotton seed samples collected from eight co-op farms in Shensi Province in 1958 showing that the percentages of seeds infected by verticillium ranged 3 to 23% with an average of 12.3%.

    (1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号...

    (1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号經初步鉴定系Verticillium albo-atrum Reinkeet Berth。(7) 特8号菌种經过接种試驗,确定其具有一定的致病力,并从再分离中获得該菌。(8) 棉籽是否能够传播黄萎病,似系一个检查方法問題。还希各方面进行测定,通过多次的检查和接种試驗始能充分确定。(9) 在分离的菌种中,除特8号菌种具有較深的菌落色泽外,还有一些菌种的顏色較淡,未須鑑定。棉籽所带的轮枝菌类型不一,还有进一步研究的必要。此外,棉籽內的輪枝菌和其他一些菌类究竟如何进入籽內,也有研究的价值。

    The investigation deals,with the nitrogen fixation of some blue-green algae isolated from the soils of rice fields.Samples of algae-bearing soils were collected from rice fields in the provinces of Hupeh,Hunan and Kiangsi.Uni-algal cultures were made first on media enriched with nitrogenous compounds to encourage vigorous growth.The uni-algal cultures were then subcul- tured onto nitrogen-free media,which effected a preliminary separation of those algae which could thrive in the absence of pre-formed nitrogenous...

    The investigation deals,with the nitrogen fixation of some blue-green algae isolated from the soils of rice fields.Samples of algae-bearing soils were collected from rice fields in the provinces of Hupeh,Hunan and Kiangsi.Uni-algal cultures were made first on media enriched with nitrogenous compounds to encourage vigorous growth.The uni-algal cultures were then subcul- tured onto nitrogen-free media,which effected a preliminary separation of those algae which could thrive in the absence of pre-formed nitrogenous compounds,from those which could not.The algae which showed a prolific growth on the nitrogen-free medium may be considered either to be capable of“fixing”(assimilating)atmospheric nitrogen,or that their nitrogen supply is coming from Azotobacter present in the culture.Our next step was to obtain bacteria-flee unialgal cultures. Two bacteriostatic methods were used:ultra-violet radiation from 300-watt quartz lamps,and treatment with streptomycin. Bacteriostatically treated uni-algal cultures were tested by inoculating sterile nitrogen-free media suitable for Azotobacter and allied organisms.Examination of these subcultures from the irradiated material showed that an intermittent radiation from our lamps of 5 minutes,repeated two or three times,was an effective bacteriostatic.Also,that Azotobacter and other micro-organisms were killed after treatment with 20 ppm.of streptomycin. The capacity for assimilating free nitrogen from the air was assessed by determining the quantity of nitrogenous compounds produced in the algal cells and in the medium,by means of the micro- Kjeldhal method.The nitrogen-fixing capacity has now been assessed on 4 isolated types of Blue- green Algae:HB 686(dnabaena azotica);HB 678(dnabaena azotica f.alpha);HB 670 (Anabaena variabilis forma);and HB 508 (Nostoc Linckia forma).The highest rate of fixation, amounting to 1.0146 rag.N/100 cc.of nitrogen-flee medium in 4 days,was attained by HB 686; the next,of 0.9382 mg.N,by HB 678,0.8614 nag.N,by HB 670;and 0.7592 mg.N,was produced under the same conditions,by HB 508.

    湖北、湖南和江西等地采集的稻田蓝藻经过分离、培养、缺氮培养初步找到可能固氮的蓝藻后,进一步得出了无菌的纯培养的蓝藻藻种,经过试验和用微量凯氏法测定其产生的氮量,确定了四种蓝藻系固氮蓝藻。在100毫升无菌无氮培养基中生长四天的结果测定,水生686固氮蓝藻(Anabaena azotica)、水生678固氮蓝藻(A.azotica forma a)、水生670固氮蓝藻(Anabaena variabilis forma)和水生508固氮蓝藻(Nastoc Linckia forma)的固氮量分别为1.0146、0.938、0.8614和0.759毫克。

     
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