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sterile
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  不育
    STUDIES ON DISPERSAL OF STERILE PEACH FRUIT BORER(Carposina nipponensis Wals.) BY USING MARK-RELEASE-RECAPTURE TECHNIQUE
    采用染色标记法研究不育桃小食心虫(Carposina nipponensis Wals.)扩散规律
短句来源
    STUDY ON STERILE EFFECT OF COTTON BOLLWORM HELIOTHIS ARMIGERA BY IRRADIATION
    棉铃虫辐射不育效应研究
短句来源
    The results showed that the movement of sterile abults in the orchard was random,80% of marked adulrs existed in the annulus of 100 meters radius,the greatest dispersal distance observed was 225 m,dispersal density (N ) would be linearly related to the reciprocal of distance (1/X),the model N =-0.67 + 402.45/X was the best.
    采用释放染色标记虫及性诱剂诱捕技术研究桃小食心虫不育成虫的田间扩散规律,结果表明:不育虫在果园内的运动方向是随机的,80%集中在距释放点100米为半径的环域内,最远距释放中心225米,扩散密度与扩散距离有明显的回归关系,符合模型N=-0.67+402.45/X。
短句来源
    According to the study on the effect of sterile inheritance of F\-1 treated with radiation,the suitable sub\|sterile dosage for cotton bollworm was 300Gy.
    根据F1 代辐射遗传不育效应的研究认为:棉铃虫当代亚不育剂量以300 Gy 为宜。
短句来源
    There were no significant difference between normal and sterile adults, females and males adults, male and female juveniles.
    不育成体鼠与正常成体鼠、正常雄性成体鼠与正常雌性成体鼠、新生雄性与雌性幼鼠之间的存活率差异都不显著。
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    STUDY ON THE STERILE DOSAGES OF ~(60)Co-γ RAY RADIATION FOR GRANARY WEEVFL CONTROL
    ~(60)Co-γ射线辐照谷象不育剂量的研究
短句来源
    In sterile soil ifested with Colletoirichum gossypii B_2 and L_29 couldsignificantly reduce seedling death rate of 31.3% and 27.4%,respec-tively,comparing with control.
    B_2处理的死苗率比对照降低31.3%,L_(29)降低27.4%。
短句来源
    the population of F060 in sterile soil appeared as 10~7-10~9-10~5.R9 survival gradually declined through all stages(60 days). PCNB was effective on populating F060 and R9 survival declination.
    而 F060在灭菌士中的群体数量呈现出10~7-10~9-10~5数量级的变化,Rg 的残存一般随时间逐渐下降,RCNB 表现出对 F060增殖和 Rg 残存降低有协同作用;
短句来源
    Seed treatment with the bacteria and the sterile metabolites reduced the disease severity by 4.8% and 27.8%, respectively.
    菌体的抑菌能力强于代谢物,浸种处理防效分别为44.8%和27.8%;
短句来源
    Tobacco seedlings potted in sterile soil were inoculated with 5 VAM fungi Glomus claroideum,Glomus mosseae,Glomus diaphanum,Glomus delhiense and Glomus versiforme in the green house.
    选用的VA菌根真菌为球囊霉属(Glomus)的G.claroideum、G.moseaeG.diaphanum、G.delhiense、G.versiforme.在温室内,灭菌盆栽条件下接种烟草。
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  sterile
Fine mapping of an Arabidopsis thaliana male sterile mutant EC2-157
      
An Arabidopsis thaliana male sterile mutant EC2-157 has been isolated using an EMS mutagenesis strategy.
      
As no male sterile genes have been reported in this region, ms157 could be a novel gene related to fertility.
      
Physicochemical Properties of Melanins Produced by the Sterile Form of Inonotus obliquus("Chagi") in Natural and Cultivated Fung
      
Physicochemical properties of pigments isolated from the naturally occurring sterile form of Inonotus obliquus(Fr.) Pil.
      
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The present report deals with the results of some studies on the longevity of the soft-rot organism, Erwinia aroideae (Towns.) Holland in soil. It is found that when the bacteria are added into a mass of non-sterile field soil in a test-tube and incubated at 28℃, they can not live longer than 14 days. This result is somewhat contradictory to those of Patel, but agrees with those of Gorlenko and Voronkeviz. A study of the number of microorganisms which, are able to be plated out in beef bouillon peptone...

The present report deals with the results of some studies on the longevity of the soft-rot organism, Erwinia aroideae (Towns.) Holland in soil. It is found that when the bacteria are added into a mass of non-sterile field soil in a test-tube and incubated at 28℃, they can not live longer than 14 days. This result is somewhat contradictory to those of Patel, but agrees with those of Gorlenko and Voronkeviz. A study of the number of microorganisms which, are able to be plated out in beef bouillon peptone agar reveals that when a known number of soft-rot bacteria is added into a soil sample, the total number of the indigenous microorganisms is increased, while the number of the introduced bacteria is rapidly decreased. After 72 hours, the total number of the indigenous microorganisms in the soil sample begins to decrease and finally reaches its original level. It is inferred that following the addition of the soft-rot organism into a soil sample, a biological equilibrium is broken and the indigenous microorganisms are stimulated to multiply rapidly. After a brief period of such an unusual multiplication of the indigenous microorganisms, the invaders are suppressed and a new equilibrium is finally established. Henceforth the number level of the indigenous microorganisms in the soil sample returns to the orginal. When the soft-rot bacteria are introduced into the soil in soil tanks regulated at different soil temperatures (and in each tank two plants of the Chinese cabbage are planted), the introduced bacteria live 11 days at 35℃, 21 days at 10℃ and 7 days at 20℃. Possibly a soil temperature at 20℃ might be close to the optimal for the activities of some antagonists of the soft-rot bacteria. When a soil sample is fertilized with the "de-oiled soybean-seed-cake" or with the "dried night soil", the elimination of the intruding bacteria is accelerated. In the plot where the de-oiled soybeanseed-cake" is applied, the soft-rot organism disappears within 24 hours, whereas in the plot of the "dried night soil", the intruding bacteria disappear within 5 days. The soft-rot bacteria in an autoclaved field soil sample live 96 days. When a quantity of manure or compost is added to the soil sample and chert autoclaved', the soft-rot bacteria introduced at such a condition live longer than 180 days. Evidently the added manure or compost in sterile condition serves as food material for the soft-rot organism.

白菜軟腐細菌(Erwinia aroideae)在不灭菌的土壤中可以存活11天至14天。土壤中的微生物总数(指可以在牛肉汁(月柬)培养基上发育的微生物)因軟腐細菌的加入而起剧烈的波动。当軟腐細菌进入土壤中后24小时,軟腐細菌的数量銳减而土壤中的其他微生物数量上昇,以后亦逐漸递減。当軟腐細菌在土壤中消失时,土壤微生物的数量又趋于平稳。軟腐細菌在栽有白菜的土壤中,土壤温度影响其存活期:土温在35℃时可以存活11天,在10℃可以存活21天,在20℃时可以活7天。豆餅及大粪干施入土壤中时可以促进軟腐細菌的消灭。施豆餅区(豆餅微粒与土壤拌匀)在24小时中软腐細菌即已消灭,而在施大粪干区軟腐細菌可以存活5天。軟腐細菌在灭菌的土壤中可以活96天,如果施以灭菌的堆肥或厩肥时,可以延长至180天以上。

Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different...

Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different culture media such as PDA, acidified PDA, cotton meal agar and rice agar. All these plates failed to show verticillium colonies. Among the fungi obtained in these plates, Fusarium, Alternaria and Colletotrichum were the most frequent. Since both Verticillium albo-atrum and other fungi were harbored within the same seed, the difference of growth rates might be considered as an important factor involved in the difficulty of the isolations: Cultures of V. albo-atrum and Fusarium sp. were transferred simutaneously to PDA plates to test their growth rates. The diameter of their colonies were measured after incubation for 12 days under 22±1℃. Those of V. albo-atrum measured 2.8 cm., while the average of the latter was 9,3 cm. Interference of the fast-growers might render a slow-grower such as V. albo-atrum to be masked in the observations and to be depressed in the isolations. A method was developed to avoid the interference. The fundamental procedures of the method are outlined as follows. 1. Plating and observation. (1) Cotton seeds being delinted with commercial surphuric acid in order to avoid the interference from lint-born contaminants. (2) Delinted seeds being washed in a flask covered with wire gauze for 24 hours under running tap water in order to get rid of bacteria. Absorption of sufficient water during washing activates the hibernating mycelium. (3) Seeds being plated on 1.7% water agar instead of nutrient agar in order to diminish the growth of the fast-growers such as Fusaria and Alternaria. The plates being incubated under 22±1℃. for 15 days. (4) The plates being uncovered and observed directly under low-power microscope in order to observe the fungi undisturbed. 2. Isolation and preservation of the Verticillium cultures. (1) Verticilliate conidiophores being located under low-power microscope. (2) The tip of a flamed needle moistened with sterile agar being introduced within the microscopic field in order to fish the spores under focus. (3) Spore suspension being made by washing the needle in a 5 cc sterile water blank. (4) Dilution plates being made by placing 1 cc of the spore suspension in a petri dish and diluting with 10 cc of cotton stem agar. (5) The plates being observed under low-power microscope after incubation for 5—7 days at 22±1℃. (6) Verticillium colonies being locatad and a bit of the margin of a colony being transferred to PDA slant. (7) The slant cultures being flooded with sterile liquid paraffin after incubation for 7—15 days at 22±1℃. The cultures preserved with liquid paraffin were able to keep alived for 2 years. Seeds collected from the infected plants in 1955, 1956 and 1957 were studied. The percentages of seeds carrying Verticillium observed in these years were 39.8, 5.92 and 9.5% respectively. One of the representative single spore colonies obtained was identified as V. albo—atrum. Inoculation and reisolation tests showed positive results. Inspection of cotton seed samples collected from eight co-op farms in Shensi Province in 1958 showing that the percentages of seeds infected by verticillium ranged 3 to 23% with an average of 12.3%.

(1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号...

(1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号經初步鉴定系Verticillium albo-atrum Reinkeet Berth。(7) 特8号菌种經过接种試驗,确定其具有一定的致病力,并从再分离中获得該菌。(8) 棉籽是否能够传播黄萎病,似系一个检查方法問題。还希各方面进行测定,通过多次的检查和接种試驗始能充分确定。(9) 在分离的菌种中,除特8号菌种具有較深的菌落色泽外,还有一些菌种的顏色較淡,未須鑑定。棉籽所带的轮枝菌类型不一,还有进一步研究的必要。此外,棉籽內的輪枝菌和其他一些菌类究竟如何进入籽內,也有研究的价值。

When seedling of corn inbreds were inoculated with isolates 76-527,76-528, 76-529 of Helminthosporium maydis in the greenhouse, the seed-lings of inbred lines with T male sterile cytoplasm were more susceptiblethan those with normal (non-sterile) cytoplasm. There was no significantdifference between the other male sterile cytoplasm and the normal, however,difference in pathogenicity were expressed in both the form and the size ofthe lesion. The virulence of the toxins produced by the isolate...

When seedling of corn inbreds were inoculated with isolates 76-527,76-528, 76-529 of Helminthosporium maydis in the greenhouse, the seed-lings of inbred lines with T male sterile cytoplasm were more susceptiblethan those with normal (non-sterile) cytoplasm. There was no significantdifference between the other male sterile cytoplasm and the normal, however,difference in pathogenicity were expressed in both the form and the size ofthe lesion. The virulence of the toxins produced by the isolate 76-529 wasfar greater than that of the toxins produced by the isolate 76-361 in in-hibiting the growth of the primary roots of inbred(T male sterile).Thedifference in virulence showed that the two physiologic races (i. e. "T" and"O"races) of H.maydis are both existing in China.

从我国不同地区采集的玉米小斑病菌(Hclminthosporium maydis),通过温室苗期接种鉴定,及其产生的毒素对玉米种子根的抑制作用,发现76-527、76-528和76-529三个分离菌系在T型细胞质雄性不育系幼苗上产生萎蔫型大病斑,而在相对应的正常系以及M、C、CA、EK、D、PS型细胞质雄性不育系幼苗上则产生坏死型小病斑。其中76-529所产生的毒素,对T型细胞质雄性不育系种子根的抑制作用远远大于相对应的正常系,因此,初步认为这些分离菌系类似国外的T小种。而76-361和76-414两个分离菌系对T型细胞质雄性不育系则无上述的特异性。因此,初步认为这些分离菌系类似国外的o小种。

 
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