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   cell period 在 肿瘤学 分类中 的翻译结果: 查询用时:0.126秒
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cell period
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  细胞周期
    The impact of cell period and cell surface antigen in cervical cancer cell lines by interferon-γ gene transfer
    γ-干扰素基因转染对宫颈癌细胞株细胞周期及表面抗原的影响
短句来源
    Protein phos-phorylated participates in regulation of cell death pathway, location, cell protection , and transition of cell period.
    研究发现蛋白磷酸化参与细胞死亡途径的调节,影响亚细胞定位、细胞保护、细胞周期进程。
短句来源
    Methods Twenty two patients with hepatic carcinomas and 6 cases with hepatic benign hyperplastic nodes took the ultrasound guided fine needle aspiration biopsy. The former took it before and after the therapy. The fresh biopsy tissue was produced into the single cell suspension which was sent for DNA detection and ratio analysis of cell period,cell proliferous ratio observation,the DNA aneuploid peak and apoptosis cell peak detection as well as their percentages using FCM.
    方法 肝癌患者 2 2例 ,肝内良性增生结节 6例 ,采用超声引导穿刺活检技术 ,对肝癌患者在超声介入治疗前后和肝内良性增生结节行病理活检的同时 ,将活检新鲜组织制成单细胞悬液应用流式细胞术 (FCM )行DNA检测及细胞周期比值分析 ,观察细胞增殖比值 ,以及是否出现DNA的异倍体峰、凋亡细胞峰及其百分比。
短句来源
    Results The radiosensitivity and radiosensitivity-related therapy was connected with post-radiosensitivity DNA damage, rehabilitation, cell period, and multigene regulation in the tumor.
    结果 肿瘤细胞对辐射的敏感性及辐射增敏与辐射后细胞内DNA损伤修复水平及细胞周期有关 ,并受多基因调控。
短句来源
    Gastric adencarcinoma cancer SGC 7901 cells were treated with allicin, and cell period, apoptosis, and telomerase activity were studied by flow cytometry and TRAP PCR ELISA assay.
    采用不同浓度的大蒜素处理SGC 790 1细胞后 ,用流式细胞仪检测细胞周期和凋亡的改变 ; 用TRAP PCR ELISA法检测端粒酶活性的变化。
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  “cell period”译为未确定词的双语例句
    To investigate the effect of allicin on cultured human gastric adenocancer cell line BGC-823 from the morphologic changes,proliferation,production of tumor markers,the second signal system,cell period ,expression of apoptotic gene by morphological observation,colorimetry,radioimmunoassay and flow cytometry Methods:Cultured human gastric adenocancer cells BGC-823(5×10 5/ml)were incubated in different concentrations of allicin(10 -5 g/L,10 -3 g/L),to be compared with a control group.
    方法 :传代培养人胃腺癌BGC - 82 3细胞 ,使细胞浓度为 5× 10 5个 /ml。 分别加入 10 -5g/L(大蒜素 1)和 10 -3 g/L(大蒜素 2 )的大蒜素 ,另设对照组 ,培养时间分别为 6h、2 4h、48h、72h。
短句来源
    The apoptosis of tumor cells and the variety of cell period were also measured to investigate the anti-tumor mechanism by IFN-γ gene therapy.
    结果 :IFN γ基因转染后 ,HeLa细胞表面的HLA DR的表达量明显增强 (P <0 .0 1)。
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  cell period
During this two-prothallial cell period before the third mitosis, plastids had large and complex fibrillar assemblies shown to be modified starch grains.
      
Comparisons of the entrainment properties of the model with experimental results show good agreement with similar modes and different entrainment ratios occurring for similar basic cycle lengths (as functions of the unperturbed cell period).
      
The duration of a one-cell period lasts about 6 hours.
      
Temporal variation of the peripheral cell period and maximum diastolic potential are also seen.
      
If s is large enough, the single-cell period distribution will be bimodal with mostly fast and slow cells, as was the case using curve D.
      
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To investigate the effect of allicin on cultured human gastric adenocancer cell line BGC-823 from the morphologic changes,proliferation,production of tumor markers,the second signal system,cell period ,expression of apoptotic gene by morphological observation,colorimetry,radioimmunoassay and flow cytometry Methods:Cultured human gastric adenocancer cells BGC-823(5×10 5/ml)were incubated in different concentrations of allicin(10 -5 g/L,10 -3 g/L),to be compared with a control group.In...

To investigate the effect of allicin on cultured human gastric adenocancer cell line BGC-823 from the morphologic changes,proliferation,production of tumor markers,the second signal system,cell period ,expression of apoptotic gene by morphological observation,colorimetry,radioimmunoassay and flow cytometry Methods:Cultured human gastric adenocancer cells BGC-823(5×10 5/ml)were incubated in different concentrations of allicin(10 -5 g/L,10 -3 g/L),to be compared with a control group.In each group the experiment was repeated for 6 times.Results:Compared with the experimental control group,(1)In the allicin groups the apoptotic rate was significantly increased apoptotic morphosis clearly shown.(2)in the allicin groups the contents of CEA,GST π were significantly decreased.(3)cAMP was significantly increased and PKC was significantly reduced in cells in the allicin groups.(4)The expression of fas and bax genes increased and that of bcl-2 gene reduced.Conclusion:Allicin induced BGC-823 cell apoptosis by affecting cAMP,PKC and increasing the expression of fas and bax genes and inhibiting that of bcl-2 gene.Allicin was proved to reduce malignancy of gastric cancer by decreasing the production of tumor markers.This study will support the clinical use of allicin to gastric cancer,and combined chemotherapy with experimental basis.

目的 :探讨大蒜素诱导胃癌BGC - 82 3细胞凋亡的作用及机制。 方法 :传代培养人胃腺癌BGC - 82 3细胞 ,使细胞浓度为 5× 10 5个 /ml。分别加入 10 -5g/L(大蒜素 1)和 10 -3 g/L(大蒜素 2 )的大蒜素 ,另设对照组 ,培养时间分别为 6h、2 4h、48h、72h。 结果 :与对照组相比 :①大蒜素可明显提高胃癌细胞凋亡率、降低PI值 (P <0 0 1或P <0 0 5 ) ,且表现出明显的细胞凋亡的形态学变化。②大蒜素可明显降低胃癌细胞CEA、GST -π的水平 (P <0 0 1或P <0 0 5 )。③大蒜素可明显提高胃癌细胞的cAMP水平 (P <0 0 1) ,降低PKC水平 (P <0 0 1或P <0 0 5 )。④大蒜素能够显著增加胃癌细胞fas基因及bax基因蛋白的表达 (P <0 0 5或P <0 0 1) ;降低bcl- 2基因蛋白的表达 (P <0 0 1)。 结论 :大蒜素可通过对第二信号系统cAMP、PKC的影响 ,增强细胞凋亡始动基因fas、凋亡促进基因bax的表达 ,抑制凋亡抑制基因bcl- 2的表达 ,增加...

目的 :探讨大蒜素诱导胃癌BGC - 82 3细胞凋亡的作用及机制。 方法 :传代培养人胃腺癌BGC - 82 3细胞 ,使细胞浓度为 5× 10 5个 /ml。分别加入 10 -5g/L(大蒜素 1)和 10 -3 g/L(大蒜素 2 )的大蒜素 ,另设对照组 ,培养时间分别为 6h、2 4h、48h、72h。 结果 :与对照组相比 :①大蒜素可明显提高胃癌细胞凋亡率、降低PI值 (P <0 0 1或P <0 0 5 ) ,且表现出明显的细胞凋亡的形态学变化。②大蒜素可明显降低胃癌细胞CEA、GST -π的水平 (P <0 0 1或P <0 0 5 )。③大蒜素可明显提高胃癌细胞的cAMP水平 (P <0 0 1) ,降低PKC水平 (P <0 0 1或P <0 0 5 )。④大蒜素能够显著增加胃癌细胞fas基因及bax基因蛋白的表达 (P <0 0 5或P <0 0 1) ;降低bcl- 2基因蛋白的表达 (P <0 0 1)。 结论 :大蒜素可通过对第二信号系统cAMP、PKC的影响 ,增强细胞凋亡始动基因fas、凋亡促进基因bax的表达 ,抑制凋亡抑制基因bcl- 2的表达 ,增加细胞凋亡率 ,并使胃癌细胞调亡 ,并抑制其增殖活性 ;并可导致胃癌细胞CEA、GST -π的合成减少 ,从而降低其水平。本研究为临床应用的中药大蒜素治疗胃癌提供了实验依据

Objective To assess the value of DNA analysis of ultrasound guided fine needle aspiration biopsy in assessment of therapeutic efficacy for hepatic carcinoma.Methods Twenty two patients with hepatic carcinomas and 6 cases with hepatic benign hyperplastic nodes took the ultrasound guided fine needle aspiration biopsy.The former took it before and after the therapy.The fresh biopsy tissue was produced into the single cell suspension which was sent for DNA detection and ratio analysis of cell period,cell...

Objective To assess the value of DNA analysis of ultrasound guided fine needle aspiration biopsy in assessment of therapeutic efficacy for hepatic carcinoma.Methods Twenty two patients with hepatic carcinomas and 6 cases with hepatic benign hyperplastic nodes took the ultrasound guided fine needle aspiration biopsy.The former took it before and after the therapy.The fresh biopsy tissue was produced into the single cell suspension which was sent for DNA detection and ratio analysis of cell period,cell proliferous ratio observation,the DNA aneuploid peak and apoptosis cell peak detection as well as their percentages using FCM.Results The DNA analysis of 22 specimens of hepatic carcinoma tissue using FCM showed that 22.7% appeared aneuploid peaks.Six cases with hepatic hyperplastic nodes did not appear the aneuploid peaks.The S period and G 2/M period ratios of the hepatic carcinoma tissue were markedly higher than that of the hepatic hyperplastic nodes (P< 0.01 ).The DNA analysis of 22 patients with the hepatic carcinoma appeared rising for the G 1 period ratio after the therapy,while the S period and G 2/M ratios falling (P< 0.01 ).The aneuploid peak disappeared in 5 cases after the therapy. 54.5% (12/22) appeared the apoptosis peak.Conclusions Besides supplying the informations of the pathological morphology,the ultrasound guided fine needle aspiration tissue could be sent for DNA analysis using FCM.It can supply efficient biological informations in assessment of the therapeutic efficacy and prognosis.

目的 探讨超声引导穿刺活检DNA分析对肝癌疗效判断的价值。方法 肝癌患者 2 2例 ,肝内良性增生结节 6例 ,采用超声引导穿刺活检技术 ,对肝癌患者在超声介入治疗前后和肝内良性增生结节行病理活检的同时 ,将活检新鲜组织制成单细胞悬液应用流式细胞术 (FCM )行DNA检测及细胞周期比值分析 ,观察细胞增殖比值 ,以及是否出现DNA的异倍体峰、凋亡细胞峰及其百分比。结果  2 2例肝癌组织经FCM检测 ,5例( 2 2 .7% )出现异倍体峰 ;6例肝内良性增生组织均未出现异倍体。肝癌组织的S期与G2 /M期比率均显著高于良性增生结节 (P <0 .0 1)。 2 2例肝癌经超声介入治疗后DNA分析显示G1期比率上升 ,S期与G2 /M期比率下降 (P <0 .0 1) ,治疗后 5例异倍体峰消失 ,并有 12例 ( 5 4.5 % )出现凋亡细胞峰。结论 采用超声引导穿刺活检 ,不仅可提供病理形态学信息 ,还可将抽吸的组织应用FCM进行DNA分析 ,为肿瘤疗效判断与预后提供有效的生物学判断依据

Objective: To study the ability of cisplatin, 5-fluorouracil and methotrexatum on inhibiting the proliferation of HeLa cell line. And to evaluate the killing mutual effects of PDD and 5-Fu on Hela cell line. Methods: To observe the effect of individual and associated drugs on HeLa cells the methyl thiazolyl tetrazolium (MTT) assay was used. The changes of cell period and the apoptosis were observed with flow cytometer (FCM) and the median-effect principle was also used. Results: Killing effects of...

Objective: To study the ability of cisplatin, 5-fluorouracil and methotrexatum on inhibiting the proliferation of HeLa cell line. And to evaluate the killing mutual effects of PDD and 5-Fu on Hela cell line. Methods: To observe the effect of individual and associated drugs on HeLa cells the methyl thiazolyl tetrazolium (MTT) assay was used. The changes of cell period and the apoptosis were observed with flow cytometer (FCM) and the median-effect principle was also used. Results: Killing effects of the individual drugs were enhanced as drug concentration increased. We also observed that the interaction of drugs was at higher concentration(CI>1) , synergism at lower concentration(CI<1). but methotrexatum had no obvious effect on HeLa cells in this experimental condition. Conclusion: The higher concentration with combination was observed in antagonism , the lower concentration in synergism.

目的:观察顺铂、5-氟尿嘧啶及甲氨喋呤在体外对人宫颈肿瘤细胞(Hela细胞)的作用。并观察顺铂、5-氟尿嘧啶配伍对Hela细胞的相互作用。方法:采用MTT测定法观察顺铂、5-氟尿嘧啶及甲氨喋呤单用及合用对Hela细胞的影响,流式细胞仪观察凋亡及细胞周期的变化。并利用中效原理判定顺铂和5-氟尿嘧啶合用的效果。结果:顺铂和5-氟尿嘧啶单独应用时随着药物剂量增加,其抑制作用也增加,两药大剂量(大于中效剂量)合用时可产生拮抗作用(CI>1),小剂量(小于中效剂量)合用时可产生协同作用(CI<1)。甲氨喋呤在本实验条件下未见明显抑瘤作用。结论:顺铂和5-氟尿嘧啶合用小剂量产生协同作用,大剂量产生拮抗作用。

 
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