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viral capsid
相关语句
  病毒壳
    In animal papillomaviruses models, vaccination against L1 and/or L2 viral capsid proteins can provide the animals an efficient protection from infection, and the mechanism of protection inculed the production of virus type-specific neutralizing antibodies.
    在模式动物中,接种L1和/或L2病毒壳蛋白可以提供有效的抗感染保护,包括病毒型特异性的中和抗体。
短句来源
    Several monoclonal antibodies(McAbs) to Epstein—Barrvirus(EB) antigen—Viral capsid antigen (VGA), membrane antigen(MA), early antigen(EA), early antigen—diffuse (EA—D), early antigen—restricted (EA—R), EB unclear antigen (EBNA), EBNA—2 and latent membrane protein (LMP) were tested using a 3 steps amplified indirect immunoperoxidase method on 5 EB cell lines.
    5种EB病毒细胞系通过三步放大间接免疫过氧化物酶方法,检测抗EB病毒抗原的单克隆抗体(McAb)—病毒壳抗原(VCA)、膜抗原(MA),早期抗原(EA)、早期弥漫性抗原(EA—D)、早期限制性抗原(EA—R)、核抗原(EBNA)、核抗原第2型(EBNA—2)及潜在性膜蛋白(LMP)。
短句来源
    Study of the Inhibiting Effect on the Expression of Viral Capsid Antigen of Epstein-Barr Virus in B95-8 Cell by Chinese Medicinal Herbs
    中草药抑制EB病毒壳抗原在体外细胞中表达的实验研究
短句来源
    Aim:To obtain sufficient Epstein-Barr viral capsid antigen and apply it to the diagnosis of Nasopharyngeal Carcinoma based on ELISA.
    获得足够的EB病毒壳抗原,建立ELISA方法用于鼻咽癌的诊断。
短句来源
    Methods:Using the recombinant DNA technique,a fragment of gene BALF_4 was cloned into vector plasmid pUR291 and a fusion protein consisting of β-galactosidase and the major polypeptide of Epstein-Barr viral capsid antigen (VCA)was expressed in E. coli. After determining the specificity of the fusion protein,the product was purified by DEAESepharose Fast Flow ion-exhange column chromatography.
    方法:将 BALF4基因克隆进入 载体pUR291,在大肠杆菌中表达EB病毒壳抗原与β-半乳糖苷酶的融合蛋白,并通过DEAE-Sepharose Fast Flow离子 交换柱层析对融合蛋白进行纯化,将纯化的蛋白用于 ELISA方法检测人血清中的IgG/VCA和 IgA/VCA抗体,用带 有pUR291质粒的宿主菌裂解液吸收待检血清中的抗β-半乳糖苷酶抗体。
短句来源
  “viral capsid”译为未确定词的双语例句
    Antibodies to EB Viral Capsid Antigen in Rhesus Monkey Sera
    恒河猴EB病毒有关抗体的检查
短句来源
    The genome of IBDV consists of two segments of dsRNA, designated A and B. The larger A segment contains two open reading frames (ORFs) which encode VP2, VP3, VP4 and VP5. The smaller genome B segment encodes VP1. VP2 and VP3 are major structural proteins of the viral capsid, forming the protective antigen of the virus.
    基因组分为A节段和B节段,A节段编码VP2、VP3、VP4、VP5 4种蛋白,B节段编码VP1。
短句来源
    Expression of the Epstein-Barr Virus P150 Viral Capsid Antigen in Escherichia Coli for the Use as Antigen in Diagnostic Tests
    EB病毒壳抗原在大肠杆菌中的表达及纯化
短句来源
    EXPRESSION OF EPSTEIN-BARR VIRAL CAPSID ANTIGEN IN INSECT CELLS AND DETECTION OF ANTIBODIES ID THE ANTIGEN
    在昆虫细胞中表达EB病毒壳抗原及其在诊断中的应用
短句来源
    The expression of Epstein-Barr virus-associated viral capsid antigen(VCA) in E.coli and application in diagnosis
    EB病毒壳抗原在大肠杆菌中的表达与应用
短句来源
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  viral capsid
The viral capsid contains three coat proteins of 35, 26 and 24?kDa, respectively.
      
IgG and IgA antibodies to viral capsid antigen (VCA), IgG antibodies to early antigen (EA), and antibodies to EBV nuclear antigen were detected at higher titers in sera of patients with uveitis than in the sera of healthy controls.
      
Full length HAV RNA and viral capsid protein VP1 were detected in 18f infected cells at earlier times post-infection than in HM175/clone 1 infected cells.
      
In this study, we describe cloning, sequencing and expression of the viral capsid proteins of three HuCVs that were identified in outbreaks of acute gastroenteritis in Virginia in 1997-1998.
      
The viral capsid protein (CP) cistron is located at the 5' terminal end of RNA2 and the Mr of CP (20?K) is close to that determined by SDS-PAGE analysis.
      
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We have attempted to produce the P150 viral capsid antigen of Epstein-Barr Virus (EBV) in Escherichia coli. This protein was found, by immun-oprecipitation, to be a clinically relevant antigen, especially for the determination of the IgA-titer in patients with Naso Pharyngeal Carcinoma(NPC).Since the expression of the entire P150 coding region was unsuccessful, we synthesized only the antigenic parts of this protein. After purification of P150 expressed by PUR290CXH580 with column chromatography (sepharose...

We have attempted to produce the P150 viral capsid antigen of Epstein-Barr Virus (EBV) in Escherichia coli. This protein was found, by immun-oprecipitation, to be a clinically relevant antigen, especially for the determination of the IgA-titer in patients with Naso Pharyngeal Carcinoma(NPC).Since the expression of the entire P150 coding region was unsuccessful, we synthesized only the antigenic parts of this protein. After purification of P150 expressed by PUR290CXH580 with column chromatography (sepharose 2B-CL), the resulting product reacts particularly well with IgA antibodies of NPC patients indicating its diagnostic value for NPC.

本文报告对EB病毒壳抗原(VCA)带有抗原决定簇的蛋白质在大肠杆菌中的表达及其纯化。以纯化后的蛋白为抗原检测人血清中VCA/IgA抗体,鼻咽癌病人及某些急性EB病毒感染人群呈抗体阳性.而对照组呈阴性。这一结果提示VCA蛋白在鼻咽癌的普查中有应用价值。

Several monoclonal antibodies(McAbs) to Epstein—Barrvirus(EB) antigen—Viral capsid antigen (VGA), membrane antigen(MA), early antigen(EA), early antigen—diffuse (EA—D), early antigen—restricted (EA—R), EB unclear antigen (EBNA), EBNA—2 and latent membrane protein (LMP) were tested using a 3 steps amplified indirect immunoperoxidase method on 5 EB cell lines. The results indicate a high specificity of McAbs, EBNA, EBNA—2 and EA. VCA and EA—R show specificity to a certain extent. MA, LMP and EA—D do not...

Several monoclonal antibodies(McAbs) to Epstein—Barrvirus(EB) antigen—Viral capsid antigen (VGA), membrane antigen(MA), early antigen(EA), early antigen—diffuse (EA—D), early antigen—restricted (EA—R), EB unclear antigen (EBNA), EBNA—2 and latent membrane protein (LMP) were tested using a 3 steps amplified indirect immunoperoxidase method on 5 EB cell lines. The results indicate a high specificity of McAbs, EBNA, EBNA—2 and EA. VCA and EA—R show specificity to a certain extent. MA, LMP and EA—D do not present expected specific reaction.

5种EB病毒细胞系通过三步放大间接免疫过氧化物酶方法,检测抗EB病毒抗原的单克隆抗体(McAb)—病毒壳抗原(VCA)、膜抗原(MA),早期抗原(EA)、早期弥漫性抗原(EA—D)、早期限制性抗原(EA—R)、核抗原(EBNA)、核抗原第2型(EBNA—2)及潜在性膜蛋白(LMP)。结果表明McAb、EBNA、EBNA—2和EA有较高特异性,VCA和EA—R有一定特异性,MA、LMP和EA—D未得到预期的特异性反应。

The mouse-adapted Lansing strain of poliovirus type 2 PV-2(L) induces fatal poliomyelitis in mice after intracerebral inoculation, while mice inoculated with Mahoney strain of poliovirus type 1 PV-1(M) show no signs of disease. Previous work had indicated that both the 5' non-transslated region of the viral genome and the viral capsid protein, neutralization antigenic site Ⅰ(N-Agl), were involved in mouse neurovirulence. In order to further explore the role of N-Agl in mouse neurovirulence, antigenic chimeras...

The mouse-adapted Lansing strain of poliovirus type 2 PV-2(L) induces fatal poliomyelitis in mice after intracerebral inoculation, while mice inoculated with Mahoney strain of poliovirus type 1 PV-1(M) show no signs of disease. Previous work had indicated that both the 5' non-transslated region of the viral genome and the viral capsid protein, neutralization antigenic site Ⅰ(N-Agl), were involved in mouse neurovirulence. In order to further explore the role of N-Agl in mouse neurovirulence, antigenic chimeras of two poliovirus strains, XF414 and XF324, were constructed. In the two strains, ten amino acids (in XF414) or 16 amino acids(in XF324)in antigenic site I in Vpl of PV-I (M) were replaced with a PV-2(L)-specific sequences using a mutagenesis cartridge. Mouse neurovirulence tests indicated that mice cerebrally inoculated with XF414 and XF324 developed poliomyelitis leading to paralysis or death. The viruses possessing antigenicity of the inoculating viruses were isolated from cerebral tissues of the paralyzed mice. The results demonstrated that N-Agl is an important determinant of poliovirus host range, and may be involved in attachment and penetration of poliovirus (in)to cells of the mouse centrol nervous system.

为探讨脊髓灰质炎病毒Ⅱ型Lansing株中和抗原位点1(N-Ag1)在对小鼠适应能力和神经毒力中的意义和作用,本实验采用DNA重组技术构建的2株抗原嵌合性(Ⅰ/Ⅱ型)PVXF414和XF324,对小鼠适应性和神经毒力进行研究。证明Ⅰ型Mahoney株中的N-Ag1被LansingⅡ型毒株的N-Ag1肽段取代后,形成的抗原嵌合性PV Ⅰ/Ⅱ型毒株脑内接种能引起小鼠中枢神经系统脊髓灰质炎病变,导致四肢麻痹或死亡。从麻痹小鼠大脑组织中分离到与接种病毒抗原性相同的活病毒,表明N-Ag1上这一小段氨基酸序列在决定PV宿主适应性中起重要作用;N-Ag1可能与病毒吸附和穿入小鼠中枢神经组织细胞有关。

 
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