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cold ischemic injury
相关语句
  冷缺血损伤
     Effect of pentoxifylline pretreatment on donor liver cold ischemic injury in the rats
     己酮可可碱预处理对大鼠供肝冷缺血损伤影响的实验研究
短句来源
  冷损伤
     Conclusions The tempera- ture of 4℃ is appropriate for hypothermic liver preservation in rats for relieve the cold ischemic injury.
     结论 4℃对于大鼠肝脏是最适保存温度,它能够明显减轻大鼠肝脏的冷损伤程度。
短句来源
     Conclusion The temperature of 4 ℃ is most appropriate for hypothermic pancreatic preservation and can considerably alleviate cold ischemic injury of rat pancreas.
     结论4℃对大鼠胰腺是最适保存温度,它能够明显减轻大鼠胰腺的冷损伤程度。
短句来源
  冷缺血损伤的
     The Protective Effects of Drugs H and X on Liver Cold Ischemic Injury
     药物H、X对大鼠肝脏冷缺血损伤的保护作用
短句来源
     Objective To investigate the efficacy of donor and liver pretreatment with pentoxifylline (PTX) simultaneously on donor liver graft cold ischemic injury.
     目的 探讨已酮可可碱预处理供者及肝脏对供肝冷缺血损伤的影响。
短句来源
  “cold ischemic injury”译为未确定词的双语例句
     Method: All of the samples for this experiment were collected from pancreas of rat with post cold ischemic injury .
     方法:以大鼠冷缺血胰腺组织作为标本,分别采用剪碎和研磨法制备胰腺组织悬液。
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    Objective To investigate the storage temperature on liver function to determine the proximal storage temperature for liver preservation.Methods Cold liver preservation and orthotopic liver transplantation (OLT)were performed in rats.The liver was preserved for 6,18 and 30 hours in UW solution at specific tempera- tures(0℃,4℃,8℃ and 12℃).OLT was performed after the preservation for 18 hours.Liver function was de- termined by measurement of AST,LDH,ATP and EC.Meanwhile,the liver was observed with microscopy.Re-...

    Objective To investigate the storage temperature on liver function to determine the proximal storage temperature for liver preservation.Methods Cold liver preservation and orthotopic liver transplantation (OLT)were performed in rats.The liver was preserved for 6,18 and 30 hours in UW solution at specific tempera- tures(0℃,4℃,8℃ and 12℃).OLT was performed after the preservation for 18 hours.Liver function was de- termined by measurement of AST,LDH,ATP and EC.Meanwhile,the liver was observed with microscopy.Re- sults 1)The levels of AST and LDH were 4℃<0℃<8℃<12℃ at the 18th and 30th hour after the preserva- tion and at the 2nd hour after the transplantation(P<0.01 or 0.05).2) The levels of ATP and EC were 4℃> 0℃>8℃>12℃ at the 18th and 30th hour after preservation and at the 20th min after transplantation(P<0.01 or 0.05).3)The histological damage was slighter in 4℃ group than in other groups.Conclusions The tempera- ture of 4℃ is appropriate for hypothermic liver preservation in rats for relieve the cold ischemic injury.

    目的研究温度对大鼠肝脏功能的影响,以确定大鼠肝脏的最适保存温度。方法采用单纯低温保存方法及建立大鼠原位肝移植模型研究保存温度对大鼠肝脏功能的影响。实验对0℃、4℃、8℃、12℃进行研究。保存时限为6,18及30h,保存18h 后行肝移植术。检测指标包括肝脏酶学、能量指标及组织形态学改变。结果①保存18,30h 的灌出液及移植后2h 血清中 AST、LDH 水平为4℃<0℃<8℃<12℃(P<0.01或P<0.05)。②保存18、30h 及移植后20min 的肝脏能量指标ATP、EC 为4℃>0℃>8℃>12℃(P<0.01或P<0.05)。③肝脏形态学示4℃组损害较其它组轻。结论 4℃对于大鼠肝脏是最适保存温度,它能够明显减轻大鼠肝脏的冷损伤程度。

    Objective To investigate the efficacy of donor and liver pretreatment with pentoxifylline (PTX) simultaneously on donor liver graft cold ischemic injury. Methods Sixty four Wistar rats were divided into two groups (donors and recipients). Then the donors were divided four groups at random: experimental group 1 (E1),experimental group 2 (E2),experimental group 3 (E3) and control group (C). The donors in the group E1 were injected intraperitonealy with PTX 50?mg/kg body weight before ...

    Objective To investigate the efficacy of donor and liver pretreatment with pentoxifylline (PTX) simultaneously on donor liver graft cold ischemic injury. Methods Sixty four Wistar rats were divided into two groups (donors and recipients). Then the donors were divided four groups at random: experimental group 1 (E1),experimental group 2 (E2),experimental group 3 (E3) and control group (C). The donors in the group E1 were injected intraperitonealy with PTX 50?mg/kg body weight before 1?h of laparotomy. In the group E2,livers were flushed with 10?ml Euro Collins solution containing 25?mg/kg PTX body weight.The donors in the group E3 were pretreated with methods of group E1 plus group E2. After livers were transplanted after preserved in 4?℃ Euro Collins solution for 6?h . Results At 30?min and 3?h ,the levels of TNF α in the experimental groups were significantly lower than in the control group (RIA),and the levels in the group E3 were significantly lower than those in the group E1,E2 ( P < 0.05 ).At 30?min ,GST levels in the experimental groups were significantly lower than those in the control group,and the levels in the group E3 were significantly lower than in the group E1 and E2 ( P < 0.05 ).At 3?h ,the levels in the experimental groups were significantly lower than those in the control group,and the levels in the group E3 were significantly lower than those in the group E2 ( P < 0.05 ).At 30?min and 3?h ,the levels of ALT in the experimental groups were significantly lower than those in the control group,levels of ALT in the group E3 were significantly lower than those in the groups E1 and E2 ( P < 0.05 ).At 24?h ,levels of ALT in the group E3 were significantly lower than those in the control group and group E2 ( P < 0.05 );Levels of AST in the experimental groups were significantly lower than those in the control group,levels in the group E3 were significantly lower than those in the groups E1 and E2 at 30?min ( P < 0.05 ).Conclusion PTX pretreatment can reduce liver injury through suppressing Kupffer cells activation and TNF α production.

    目的 探讨已酮可可碱预处理供者及肝脏对供肝冷缺血损伤的影响。方法 Wistar大鼠按处理方法的不同分为 4组 :对照组、供者预处理组 (实验 1组 )、供肝预处理组 (实验 2组 )、供者及供肝联合预处理组 (实验 3组 ) ,预处理使用己酮可可碱。各组动物均在供肝冷保存 6h后行原位肝移植 ,门静脉血流恢复后第 30min、3h及 2 4h取门静脉血测定肿瘤坏死因子 (TNF α)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶 (AST)及谷胱甘肽S转移酶 (GST)的水平。结果 门静脉复流后 30min、3h时血清TNF α水平 ,各实验组明显低于对照组 ,实验 3组显著低于实验 1组及实验 2组 (P <0 .0 5) ;30min及 3h时血清GST水平 ,各实验组明显低于对照组 (P <0 .0 5) ,实验 3组显著低于实验 1组及实验 2组 (P <0 .0 5) ;30min及 3h时血清ALT水平 ,各实验组明显低于对照组 ,实验 3组显著低于实验1组及实验 2组 (P <0 .0 5) ;2 4h时前述各指标各实验组明显低于对照组 ,实验 3组显著低于实验 2组 (P <0 .0 5) ;30m...

    目的 探讨已酮可可碱预处理供者及肝脏对供肝冷缺血损伤的影响。方法 Wistar大鼠按处理方法的不同分为 4组 :对照组、供者预处理组 (实验 1组 )、供肝预处理组 (实验 2组 )、供者及供肝联合预处理组 (实验 3组 ) ,预处理使用己酮可可碱。各组动物均在供肝冷保存 6h后行原位肝移植 ,门静脉血流恢复后第 30min、3h及 2 4h取门静脉血测定肿瘤坏死因子 (TNF α)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶 (AST)及谷胱甘肽S转移酶 (GST)的水平。结果 门静脉复流后 30min、3h时血清TNF α水平 ,各实验组明显低于对照组 ,实验 3组显著低于实验 1组及实验 2组 (P <0 .0 5) ;30min及 3h时血清GST水平 ,各实验组明显低于对照组 (P <0 .0 5) ,实验 3组显著低于实验 1组及实验 2组 (P <0 .0 5) ;30min及 3h时血清ALT水平 ,各实验组明显低于对照组 ,实验 3组显著低于实验1组及实验 2组 (P <0 .0 5) ;2 4h时前述各指标各实验组明显低于对照组 ,实验 3组显著低于实验 2组 (P <0 .0 5) ;30min时血清AST水平 ,各实验组明显低于对照组 ,实验 3组显著低于实验 1组及实验 2组 (P <0 .0 5)。结论 己酮可可碱预处理对供肝的冷缺血再灌注损伤有一定的保护作用

    Objective: To explore the mechanical methods for making pancreatic tissue into single cell suspension.Method: All of the samples for this experiment were collected from pancreas of rat with post cold ischemic injury . Single cell suspension got by “cut” and “homogenator” way were detected with FCM(Flow cytometry). The cell viability was assessed by typan blue exclusion;The morphologic changes of cell were observed. Result: Cells got by the method of “cut” were intact,the viability was good,the percentage...

    Objective: To explore the mechanical methods for making pancreatic tissue into single cell suspension.Method: All of the samples for this experiment were collected from pancreas of rat with post cold ischemic injury . Single cell suspension got by “cut” and “homogenator” way were detected with FCM(Flow cytometry). The cell viability was assessed by typan blue exclusion;The morphologic changes of cell were observed. Result: Cells got by the method of “cut” were intact,the viability was good,the percentage of APO and CV vales were low. Conclusion: The method of“cut”with sissors for making renal tissue into single cell suspension was better than that of“homogenator”.

    目的:寻找更好的胰腺组织单细胞悬液制备方法。方法:以大鼠冷缺血胰腺组织作为标本,分别采用剪碎和研磨法制备胰腺组织悬液。经台盼蓝染色测细胞存活率,并进行流式细胞术分析。结果:剪碎法细胞形态较完整、存活率高;两种方法细胞凋亡水平(APO)、CV值有显著性差异(P<0.01)。结论:胰腺组织单细胞悬液制备剪碎法优于研磨法。

     
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