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toxin-protein
相关语句
  毒素蛋白
     Crude toxin extraction was obtained by a serial procedure of filter,concentration,centrifugal and dialysis. Composition of the extraction was analyzed. The results showed that 14 amino acids existed in the toxin-protein of the Verticillium dahliae of eggplant wilt,among which acidic amino acid accounted for 17.51% and the alkaline amino acid was about 10.46% percent.
     试验用Czapck's培养液振荡培养茄黄痿病菌(Verticiliumdahliae),通过真空抽滤、浓缩、离心和透析,得到病菌粗毒素,对其氨基酸组份分析,结果表明:茄黄萎病菌粗毒素蛋白中含有14种氨基酸,其中酸性氨基酸占17.5%,碱性氨基酸占10.46%。
短句来源
     The trial was conducted with the culture filtrate of Verticillium wilt pathogen(Verticillium dahliae)to test the influence of toxin-protein on the permeability of root cell membrane in egg-plant. The results showed that the permeability of root cell membrane was increased with the time of treatment and with the concentration of toxin after toxin treatment in seedlings of Egg-plant and became stable finally.
     本试验用茄子黄萎病菌 (Verticillium dahliae)培养滤液测试了病菌毒素蛋白对茄根部细胞膜透性的影响 ,结果表明 ,茄幼苗用病菌培养滤液处理后 ,其根部细胞膜透性随处理时间延长及毒素浓度的增加而增大 ,最后趋于平稳
短句来源
  病菌毒素蛋白
     The trial was conducted with the culture filtrate of Verticillium wilt pathogen(Verticillium dahliae)to test the influence of toxin-protein on the permeability of root cell membrane in egg-plant. The results showed that the permeability of root cell membrane was increased with the time of treatment and with the concentration of toxin after toxin treatment in seedlings of Egg-plant and became stable finally.
     本试验用茄子黄萎病菌 (Verticillium dahliae)培养滤液测试了病菌毒素蛋白对茄根部细胞膜透性的影响 ,结果表明 ,茄幼苗用病菌培养滤液处理后 ,其根部细胞膜透性随处理时间延长及毒素浓度的增加而增大 ,最后趋于平稳
短句来源
  “toxin-protein”译为未确定词的双语例句
     Vector Construction with Bt Gene for Sugar Beet Chloroplast Transformation and Expression Toxin-protein
     Bt基因甜菜叶绿体转化载体构建及毒蛋白表达
短句来源
  相似匹配句对
     Phytopathogenic Toxin
     植物病原毒素
短句来源
     Cholera toxin
     霍乱毒素
短句来源
     HEAT-TOXIN IN APOPLEXY
     中风热毒论
短句来源
     Preparation of ~3H-T-2-toxin
     氚标记T-2毒素的制备
短句来源
     The Insecticide Toxin Protein from Xenorhabdus and Photohabdus
     致病杆菌属和光杆状菌属细菌杀虫毒素蛋白
短句来源
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Enzyme-Linked immunosorbent assay was used to detect and quantitate the insecticidal crystal toxin proteins of B. thuringiensis HD-1 and commercial preparations. The minimal detectable value by indirect method of ELISA was 7.79 ng/0.15ml. The total crystal protein in standard sample HD-1-S-1980 and a commercial Bt emulsion (produced by Hubei Academy of Agricultural Sciences) was measured to be 108.06 mg per gram of dry powder and 4.57-6.17 mg per ml of emulsion, respectively. With these two values, the relative...

Enzyme-Linked immunosorbent assay was used to detect and quantitate the insecticidal crystal toxin proteins of B. thuringiensis HD-1 and commercial preparations. The minimal detectable value by indirect method of ELISA was 7.79 ng/0.15ml. The total crystal protein in standard sample HD-1-S-1980 and a commercial Bt emulsion (produced by Hubei Academy of Agricultural Sciences) was measured to be 108.06 mg per gram of dry powder and 4.57-6.17 mg per ml of emulsion, respectively. With these two values, the relative toxicity potency of a commercial emulsion can then be calculated.

用酶联免疫吸附测定(ELISA)检测Bt伴孢晶体毒素蛋白质,最低可检值7.79ng/0.15ml,灵敏、精确,具高度专化性。测定标准品HI—1—S—1980和Bt乳剂产品中伴孢晶体蛋白质含量,分别为108.06mg/g粉剂和4.57—6.17mg/ml乳剂。用标准品与待测样品(Bt乳剂)的晶体蛋白质含量,就可计算出待测样品的相对毒力效价。ELISA与昆虫生测之间有良好的相关性。

The delta-endotoxin protein of Bacillus thuringiensis subsp. kurstaki HD-1 was isola-ted from parasporal crystals. The purified toxin protein had a molecular weight of 68000showed on SDS-PAGE gel. Monoclonal antibody against delta-endotoxin protein was ra-ised by the hybridoma technique and detected by an indirect enzyme. linked immunosorb-ent assay (ELISA).High title of the monoclonal antibodylin supernatant of cell culture hadbeen detected after neatly half year culture in vitro. The high specificity of the...

The delta-endotoxin protein of Bacillus thuringiensis subsp. kurstaki HD-1 was isola-ted from parasporal crystals. The purified toxin protein had a molecular weight of 68000showed on SDS-PAGE gel. Monoclonal antibody against delta-endotoxin protein was ra-ised by the hybridoma technique and detected by an indirect enzyme. linked immunosorb-ent assay (ELISA).High title of the monoclonal antibodylin supernatant of cell culture hadbeen detected after neatly half year culture in vitro. The high specificity of the antibodycould be used for detection of delta-endotoxin gene expression in genetic engineeredproducts.

从苏云金芽孢杆菌HD-1伴胞晶体中分离得到的毒素蛋白经过Sepharose 6B和DEAE-Sephadex DE-52得到了纯化,纯化后的蛋白在SDS-PAGE上呈现一条带,其分子量约为68 000。用纯化蛋白作为抗原免疫小鼠,通过杂交瘤技术建立了抗HD-1 δ-内毒素蛋白的单克隆抗体杂交瘤细胞株。经过半年的体外培养,大多数细胞株仍能持续分泌高滴度的单克隆抗体。文中还讨论了抗体的特异性及理化特性。此单克隆抗体细胞株的建立将为植物基因工程产物的检测提供有力工具。

Bacillus Sphaericus-10 (BS-10) is a pathogenic bacterium on the larvae of mosquito.It can kill the Culex pipiens pallens Coquillett highly. The serrtype H5a5b was classifledfrom the surburb, Yangzhou in 1982. It is similar to the strains 1593, 2362 recomended by WHO in the characteric of physiology and biochemistry But its toxicity is 2.78—4.2 times than the strain 1593 2.78 times more than the strain 2362. The molecular weight of toxin protein of BS 10 is 45KD), the proteinmainly exists in the sporange andspore...

Bacillus Sphaericus-10 (BS-10) is a pathogenic bacterium on the larvae of mosquito.It can kill the Culex pipiens pallens Coquillett highly. The serrtype H5a5b was classifledfrom the surburb, Yangzhou in 1982. It is similar to the strains 1593, 2362 recomended by WHO in the characteric of physiology and biochemistry But its toxicity is 2.78—4.2 times than the strain 1593 2.78 times more than the strain 2362. The molecular weight of toxin protein of BS 10 is 45KD), the proteinmainly exists in the sporange andspore cell of BS-10 with a square parasporal cristaline. When the larve of mosquito ate some spores, at most, 6 hr. later it would die, and the death is right proportion to the quantity of diet, and the younger the more sensltive. Its control spectrumas follows: Culex pipiens Coquillett C. pipiens quinuefascitatus Say C.trilaencorhynchus Giles Anopheles sin ensis Wiedean Ae. aegypti Linnaeus. Usage of BS-10 is 3-5ml/m~2, 80% of the death after 24 hr.; 95-100% after 48 hr., generaly 15-25Days of persistence. What's more, the product of BS-10 is no harm to the human, beast, poultry, and aquaticlife, and no pollution of environment. Key words: Bacillus sphaericus-10 larvae of mosquito BioIogical characteristic Product of BS-10

球形芽孢杆菌BS-10(Bacillus Sphaericus-10)是蚊幼虫的一种病原体,对库蚊等蚊幼虫具有极高的毒杀作用。1982年从江苏扬州市郊区孳生地分离到,经鉴定属血清5_a5b型,其生理生化特性与世界卫生组织(WHO)推荐的1593、2362菌株相似,但其毒力比1593菌株高1.78—3.2倍,比2362的发酵毒力提高1.79倍。BS-10的杀蚊毒素蛋白的分子量为45KD,主要分布于孢子囊、芽孢壁内,并有菱形的伴孢晶体毒素。当蚊幼吞食一定数量的芽孢后即中毒死亡,吞食数与死亡速度成正相关。最快6h可毒杀,但蚊幼的龄期越小越敏感。它的杀蚊谱为:淡色库蚊>致乏库蚊>凶小库蚊>三带喙库蚊>中华按蚊>埃及伊蚊。BS-10灭幼剂使用量为每m~2投药3~5ml,24h蚊幼死亡率为80%左右,48h为95~100%;随着用药量增加,持效期延长,一般为15~25天。BS-10对人畜、禽及水生生物安全无毒,不污染环境。

 
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