However, the addition of 0.05 mmol/L acetate, 1 mmol/L phenylalanine,0.1 mmol/L benzoate and 6 g/L fructose into the cultures on the 15th day of culture dramatically increased taxol yield by 181.89%.
the addition of 0.05 mmol/L acetate, 0.05 mmol/L phenylalanine,0.1 mmol/L benzoate with fructose ,increased taxol yields by 49.36%,13.18% and 64.26%,respectively.
The induction of phenylalanine ammonia lyase(PAL) in Rhodotorula rubra As 2.279 and transformation of L phenylalanine from trans cinnamic acid(t Ca) were studied.
With the combination treatment of 30μmol/L phenylalanine and 218μmol/L methyl jasmonate in the dark in suspension cultures of E,the anthocyanin content and production in suspension culture of E was 5.89-fold and 4.30-fold of the controls,respectively,and all the variation coefficients of biomass and anthocyanin accumulation were lower than those of the controls in 5 successive subcultures.
According to the result, when l 000 μmol/L phenylalanine was fed, both levels of L-epicatechin and D, L-catechin in cells raised about 40% and the total level of EtOAc extrat increased 66%.
whereas, the flavonoid content could get back to the 11% when the cells grew in the reactor with the modified MS medium containing 28mg/L NaAc and 60mg/L L-phenylalanine during the later culture period, and the cell density could reach 21g (dry weight)/L.
Using NH+ 4-form positive ion exchange resin to extract L-phenylalanine from its mother liquor, we found that the best pH value of the mother liquor was 1.0 and 95% of L-phenylalanine was obtained by desorption solution of 0.15mol·L -1NH 3·H 2O.
The amount of aromatic amino acids such as L-tryptophan, L-tyrosine and L-phenylalanine was important for the accumulation of D-ribose, and its amount need to be controlled precisely.
In this study, RP-HPLC with fluorescence detection and Fluorescence spectrum (excitation 277 nm and emission 306 nm) was used to detect hydroxyl radical generated by Cu(Ⅱ)-H_2O_2 (Fenton like reaction) system, using a fluorescence probe, L-phenylalanine, which could be converted to highly fluorescent L-tyrosine in the presence of ·OH.
The proposed method has been applied to the simultaneous determinations of the binary mixture of 0.974-2.026 X 10-2g/L nitrate and nitrite, the ternary mixture of 1.009-5.325 X 10"2g/L phenol, catechol and quinol, 1.840-3.888 X 10"3g/L histidine, phe and trytophan.
Results: ① 0.2 mmol·L -1 and 2.0 mmol·L -1 L-tyrosine significantly inhibited 3β-HSD activity. ② 0.2 mmol·L -1 L-tyrosine exerted different effects on 3β-HSD activity at different concentrations of pregnenolone (Ph).
Using Corynebacterium glutamicum TQ2223(Phe~-+Tyr~-+5MT~r+SG~r+5FT~r+CIN~r) as L-tryptophan producing strain, the influence by different feeding modes of(NH_4)_2SO_4, corn hydrolysate and L-Tyr and L-Phe was studies in this paper.
Experiments indicated that phenylalanine (Phe) inhibited phenylalanine ammonia-lyase (PAL) activities when its concentrations was more than 2.5 mmol/L, whereas when it was equal or lower than 2.5 mmol/L,the activities were enhanced. The treatment with 1.25 mmol/L of Phe showed the activities of PAL were greatly increased.
The application of 10 mmol/l phenylalanine replacing 10 mmol/l raffinose leads to a rapid (within 30 s) depolarisation of PDpt to 50±5% of its control value and to a delayed (within 12 min) recovery to 95±5% of control.
Biogenic elicitors (chitosan and its complex with salicylic acid) and an immunosuppressor (laminarin) were shown to increase the activity of L-phenylalanine ammonia lyase (EC 4.3.1.5) and protein synthesis in potato tubers.
1.The free amino acids and amino acid compositions of proteins in the rice stem and in the hemolymphs of Tryporyza incertellus Walker and Tessaratoma papillosa Drury were determined by two dimensional paper chromatography.2.Aspartate-glutamate transaminase,alanine-glutamate transaminase,ornithine transaminase,and r-aminobutyrate-glutamate transaminase were found active in the fat body of Tryporyza incertulas Walker.Fat body of Tessaratoma papillosa Drury was shown to be able to catalyse transamination react...
[Pt(C_(11)H_(15)NO_2)_2 Cl_2] is monoclinic, with space group P2_1. Z=2, and lattice parametes α=13.555(6)A, b=6.487(3)A, c=15.588(5)A, β=109.42(3)A. The structure was determined by heavy-atom method and refined by "blocked cascade" full-matrix leastsquare refinement technique to R=0.0617, R_w=0.0575. The atom Pt(Ⅱ) has approximate square coordination, with bond lengths Pt-N 2.065(14), 2.084(14)A; PtCl 2.285(7), 2.312(8)A. The structure contains layers of molecules hydrogen bonded together through N-H……O.
In Hep A ascites hepatoma (Hep A), addition of fructose-1, 6-diphosphate (FDP) or phosphoenol pyruvate (PEP) brought about a marked Crabtree effect, but in normal mouse liver, this effect was seen only when PEP was used as substrate, and the values of the Crabtree effect were much less than that in Hep A. The pyruvate kinase (PyK) activity in Hep A was 4.7 times as high as that in normal mouse liver. During studies on the relationship between PyK and the Crabtree effect, the following facts were revealed: (...
l苯丙氨酸
l phenylalanine
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