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recovery of activity
相关语句
  活性恢复
     This catalyst has been used in a urea plant with feedgas containing sulfur [total sulfur<3 mg/m 3 (standard state)] for 18 months, and H 2 can be reduced to <50×10 -6 . This catalyst has the characteristics of good sulfur tolerance and quick recovery of activity after sulfur poisoning.
     该催化剂在原料气含硫 [总硫 <3mg/m3 (标态 ) ]的尿素厂已应用了 18个月 ,H2 可降至 <5 0× 10 -6。 该催化剂具有耐硫性能好、硫中毒后活性恢复快的特点。
短句来源
     When dark-treated culture (in air for 12 hrs) was reilluminated, the recovery of activity was depressed by chloramphenicol, NH_4 or by 38—40℃/air. This implies that the preformed nitrogenase was inactivated by atmo-spheric oxygen in darkness.
     在黑暗12小时后,复光时的活性恢复被氧霉素、氯化铵或38—40℃空气氧所阻遏,黑暗中生成的酶易被氧失活。
短句来源
  活力收率
     And then the enzyme was further purified through gel filtration on Superdex75 and cation exchange on Source 30S. The final recovery of activity was about 37%.
     又经Superdex 75凝胶过滤和Source 30S阳离子交换两步纯化后得到纯酶 ,最终酶活力收率约 37%。
短句来源
  “recovery of activity”译为未确定词的双语例句
     The recovery of activity was 45.2% with specific activity of 15.6 units/nig protein.
     活力回收45.2%,比活力15.6单位/毫克蛋白;
短句来源
     The properties of this new concentrates were compared with those of the concentrates obtained by foreign DEAE Sapharose CL 6B. Results The recovery of activity was 93.67±2.56%( n =6) and 88.71±2( n =6),respectively.
     结果 两步的活性回收率分别为93.6 7± 2 .5 6 % (n=6 )和 88.71± 2 .39% (n=6 )。
短句来源
     The purified enzyme is 4563. 3 unit per mg protein,so that it is purified by 51 fold, the rate of recovery of activity is 22.3%.
     纯化后的酶为4563.2u/mg·蛋白,纯化了51倍,活力回收为22.3%。
短句来源
     The recovery of activity was 22.4-42.3% from 20 liters of plasma. By fur- ther purification, the recovery of activity and antigen was 26.3% and 20.3% respec- tively, the specific activity was 9.9U/mg.
     从20升血浆中获得的AT-Ⅲ活性回收率为22.4%—42.3%,进一步纯化后的AT-Ⅲ活性及抗原回收率分别为26.3%和20.3%,其比活性为9.9U/mg蛋白,在聚丙烯酰胺凝胶电泳图谱上显示单一的区带;
短句来源
     Before add 1.5 times ethanol as the volume of TGase, modify pH of TGase to 7.5 to 8.0. The total recovery of activity of the process is about 85 %.
     将超滤后的酶液pH值调节到7.5至8,加入体积为酶液体积1.5倍的冷乙醇沉淀,酶活收率达到85%左右。
短句来源
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  相似匹配句对
     ON MINERAL RECOVERY
     谈选矿回收率问题
短句来源
     Recovery factor
     采收率
短句来源
     On the Right of Recovery
     归入权问题之研究
短句来源
     Recovery of "the Other"
     “他者”的复苏
短句来源
     The activity of E.
     淀粉酶活性在50℃,pH 6.0条件下最高.
短句来源
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  recovery of activity
Addition of 0.01% polysorbate 80 to the formulation resulted in complete recovery of activity, irrespective of the sucroseglycine ratio or annealing.
      
Recovery from depression evoked by the first stimulus took place more suddenly in neurons in the central part of the visual field, and initial recovery of activity was more complete than in peripheral neurons.
      
Recovery of activity took place gradually, without postinhibitory facilitation.
      
Recovery of activity took place gradually, without postinhibitory facilitation.
      
Substantial recovery of activity occurs (over a 3-4 week period) when inhibited enzyme is sedimented and resuspended in fresh buffer.
      
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1. Benzyated insulins were prepared by three different methods. Reduction by metallic so-dium in liquid ammonia followed by benzylation with benzyl chloride gave a fully benzylatedproduct with no intact disulphide bond left. 2. Reduction of insulin, mixtures of S-sulphonates of A and B chains as well as benzylatedinsulin by sodium in liquid NH_3, followed by re-oxidation of the reduced products in a slightlyalkaline solution all resulted in the regeneration of insulin activity. In the casc of benzylated in-sulin,...

1. Benzyated insulins were prepared by three different methods. Reduction by metallic so-dium in liquid ammonia followed by benzylation with benzyl chloride gave a fully benzylatedproduct with no intact disulphide bond left. 2. Reduction of insulin, mixtures of S-sulphonates of A and B chains as well as benzylatedinsulin by sodium in liquid NH_3, followed by re-oxidation of the reduced products in a slightlyalkaline solution all resulted in the regeneration of insulin activity. In the casc of benzylated in-sulin, the recovery of activity was usually 1-2% whereas with insulin or S-sulphonated A and Bchains 5-10% activity could be recovered. 3. The regeneration of insulin activity from the fully benzylated derivative suggests that theeventual synthesis of insulin can be effected from the chemically synthesized chains. 4. The course of the oxidation of sulfhydryl groups and the re-appearance of biological activityhas been followed and the results obtained have been discussed.

(1)应用三种不同的方法制得了胰岛素苄基衍生物。胰岛素如果用钠-氨还原再与氯化苄作用,可以得到不再有完整二硫键存在的充分苄基化的产物。(2)将胰岛素、S-磺酸型A及B链混合物或者胰岛素苄基衍生物,在钠-氨内还原,然后在稀碱溶液中重氧化,都可以使胰岛素活力再生。用胰岛素或S-B磺酸型A及B链混合物时,经过此处理则活力可以恢复5—10%。用胰岛素苄基衍生物时则一般活力恢复1—2%。(3)从充分苄基化了的胰岛素衍生物能恢复活力这一事实说明可以通过人工合成的A和B链最后合成胰岛素。(4)观察了A和B键混合物巯基氧化和活力再生的过程,并进行了讨论。

The ultraviolet difference spectrum of urea-denatured trypsin against trypsin showed three negative peaks at 292, 286, and 236 mμ and a flat maximum in the 250—270 mμ region. In the course of inactivation in urea and that of reactivation of the denatured enzyme, changes in enzymatic activity were accompanied by changes in ultraviolet absorption spectra. During denaturation the rate of decrease of absorption at 236 mμ was faster than that of the decrease in activity but upon dilution of a solution of trypsin...

The ultraviolet difference spectrum of urea-denatured trypsin against trypsin showed three negative peaks at 292, 286, and 236 mμ and a flat maximum in the 250—270 mμ region. In the course of inactivation in urea and that of reactivation of the denatured enzyme, changes in enzymatic activity were accompanied by changes in ultraviolet absorption spectra. During denaturation the rate of decrease of absorption at 236 mμ was faster than that of the decrease in activity but upon dilution of a solution of trypsin in concentrated urea, the recovery of activity was faster than the increase in absorption at 236 mμ.The kinetic behaviour of the change in absorption at 236 mμ during denaturation was consistent with the simultaneous occurrence of two first order reactions. The rate constant of the slower reaction was found to be identical with the rate constant of the decrease in activity which was also first order. The specific substrate, p-tosyl-L-arginyl methyl ester, partially protected both the change in light absorption and the decrease in activity. These results seem to indicate that only part of the trypsin molecule is required for its activity and the conformation of the essential part is more stable than that of the test of the molecule.The thermodynamic parameters of the activation processes of the above changes have also been determined and their significance discussed.

胰蛋白酶的尿素差示光谱在292、286和236mμ有三个负的差吸收峯,和在260mμ范围有一较为平坦的正峯。在变性过程和恢复过程中伴随着活力的丧失和恢复都有差吸收的变化。变性过程差吸收的变化略快于活力的变化,而恢复过程活力的变化又快于差吸收的变化。变性过程可以分解为两个一级反应,仅其中较慢的反应与活力的变化一致,另一反应则与活力无关。底物对甲苯磺酰精氨酸甲酯,对变性过程差吸收和活力变化都有保护作用。由以上结果看来,胰蛋白酶表现其活力并不要求整个酶分子具有完整的空间构型并且它们的活性中心部分的空间构型是较为稳定的。此外本文还研究和计算了变性与恢复过程的热力学常数,并且对它们进行了讨论。

The S-sulphonates of A and B chains obtained by splitting insulin with Na_2SO_3 and Na_2S_4O_6 in the absence of urea were separated by repeated precipitation of the B chain at pH 6.4. From the supernatant solution, the A chain was further purified by zone electrophoresis on cellulose powder at pH 7. When the S-sulphonates of A and B chains were mixed, reduced and reoxidized the final level of activity regenerated varied with the ratio of the chains; the highest recovery of activity was at a molar ratio...

The S-sulphonates of A and B chains obtained by splitting insulin with Na_2SO_3 and Na_2S_4O_6 in the absence of urea were separated by repeated precipitation of the B chain at pH 6.4. From the supernatant solution, the A chain was further purified by zone electrophoresis on cellulose powder at pH 7. When the S-sulphonates of A and B chains were mixed, reduced and reoxidized the final level of activity regenerated varied with the ratio of the chains; the highest recovery of activity was at a molar ratio of A and B chains of 1.5:1. The final reoxidation product thus obtained usually had an activity of 12 international units/mg of total protein, about 50% of that of crystalline insulin.The optimal reduction and reoxidation conditions for activity recovery established so far were: 1) reduction with 28-fold excess of mercaptoethanol at 100℃, pH 5 for 6min. 2) precipitation and washing of the reduced chains at pH 3.8.3) reoxidation at 3-5℃ in pH 10.6 glycine buffer at a protein concentration of 5mg/ml.The velocities of the reduction and reoxidation of respectively the S-sulphonated and reduced B chain alone were consider(?)bly slower than those of the corresponding A chain derivatives alone but were raised to the level of those of the A chain derivatives in mixtures of the chains. These and the regeneration of about 50% of insulin activity from the reduced chains under favourable conditions suggest strongly that some interactions between the chains, probably through secondary bonds, take place in solution. These interactions may play an important role in the successful resynthesis of insulin from its chains.

利用二者溶解度的不同可以将无脲条件下拆开的胰岛素初分而得到较纯的S-磺酸型A及B链,再在中性pH用纤维素板电泳可以制得纯的S-磺酸型A链。将经过分离提纯的A及B链按不同比例重新混合还原重氧化结果表明活力恢复程度与A与B链的比例有关,A比B过量对活力恢复有利,A:B=1.5:1时活力恢复最高,可达天然胰岛素的50%左右。本文还对从S-磺酸型A及B链重合成胰岛素最有利的还原及重氧化条件进行了探讨,除其他条件外重氧化的最适pH为10.6。B链单独存在时其还原及重氧化速度均明显地较A链的相应速度为低,但二者共同存在时的还原及重氧化速度却与A链单独存在时相同,表明在溶液中自由A及B链间存在着对恢复活力有重要影响的相互作用,可能是通过次级链作用的相互作用。

 
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