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on analysis
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  on analysis
The structural elucidation is based on analysis of spectroscopic data.
      
Based on analysis of the well drilling core from Subei basin, the authors conclude that during the Late Cretaceous and Paleocene, Subei basin was linked with the sea and the deposit was affected by transgression.
      
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Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter...

Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter with hydrogen sulphide,followed by paper electrophoresis and paper chromatography.The purified product has been separated into four flavin-adenine peptides with different amino acid contents.One fraction with comparatively high mobility on paper electrophoresis and containing 12 amino acids(hydrolyzed in 6 N HCl) has an absorption spectrum with maxima at 265,350 and 450 mμ(compared with 260,375 and 450 mμ of FAD),the ratio of E_(260) and E_(450) mμ is equal to 3.87.The other three fractions has similar absorption spectra as that of the first,except for a slight shift of the 265 mμ maximum to 270 mμ.All the four flavin-adenine peptides contain cysteine and show a greenish yellow fluorescence in the u.v.light.The fluorescent intensity of the prosthetic group varies with pH and exhibits a maximum at pH 2.9.All fractions are inactive in the D-amino acid oxidase test and give on analysis 1 mole of adenine and 2.5 moles of phosphorus per mole of flavin.The pentose flavin ratio was much higher than that of FAD. Photolysis of the flavin-adenine peptides in alkaline solution yields a product which is insoluble in chloroform after acidification.Removal of the adenine results in the formation of flavin peptides.These facts indicate that the peptide chain is linked to the isoalloxazine nucleus of the prosthetic group.It is known that the absorption peak at 375 mμ of either FAD or FMN shifts to about 355 mμ at pH 12 due probably to the enolization of the keto group at the 2 or 4 position resulting in a redistribution of double bonds in the isoalloxazine ring system. In contrast our flavin-adenine peptide has the corresponding absorption maximum at 350 mμ which shows little positional shift at pH 12.This seems to suggest that the linking of the peptide chain to the isoalloxazine nucleus affects the enolization of the-NH-CO-,which may probably be the site of the linkage. The iron content increases with the specific activity of the enzyme during purification.Iron in the purified enzyme is present in the reduced state.It is firmly bound to the enzyme and can not be removed by prolonged dialysis against phosphate buffer or tris-hydroxymethyl-amino-methane buffer.Enzymatic activity is lost during prolonged incubation with o-phenanthroline or α,α'- dipyridyl and can not be recovered by incubation with Fe~(2+) or Fe~(3+).These experiments de- monstrate a close relationship between enzyme activity and the iron present in the enzyme molecule. The enzyme activity is lower in borate than in phosphate buffer.When 40 mM ethylenedi- aminetetraacetic acid is added to the borate buffer,the enzyme activity is raised almost to the level of that observed in the same concentration of phosphate buffer.The effect of EDTA and phosphate,when present together,is somewhat higher than of either alone.Alanine has a similar effect'as EDTA.

(一)用结晶胰蛋白酶及结晶胰凝乳蛋白酶处理净化的水溶性琥珀酸脱氢酶,经过对甲酚抽提,硫酸汞沉淀,硫化氢分解及纸电泳纸层析等方法净化得到四种带有不同肽链的腺嘌呤异咯嗪核苷酸。从它们的组成成份的分析以及它们的性质的观察,我们认为它们与已知的腺嘌呤异咯嗪二核苷酸略有不同。肽链是连接在异咯嗪上,其连接方式异于一般异咯嗪蛋白。肽链部份的氨基酸组成的分析结果,证明它们都含有半胱氨酸。(二)琥珀酸脱氢酶中的铁处于还原状态。铁与酶朊紧密结合,它与酶活力有密切关系。(三)无机磷可增加琥珀酸脱氢酶的活力,但琥珀酸脱氰酶的活力并不是必需依靠无机磷的存在,乙二胺四乙酸与丙氨酸也有类似无机磷的作用。

Based on field test results, the author investigates the loading characteristic of the elasto-plastic frozen wall according to Mohr-Coulomb plastic condition. Emphasis are given on analysis of the development of the plastic region in the frozen wall with various thickness under increasing load. The relationship between the load and the dimensions of frozen wall, the radius of the plastic region and the reaction of the shaft lining are discussed. The rational extent of the plastic region is found.

本文在试验基础上,根据库仑-莫尔塑性条件,研究了弹塑性冻结壁的承载性能,着重分析了在外载增长情况丁不同厚度冻结壁的塑性区扩展状况。对冻结壁几何尺寸、塑性区扩展半径、井壁抗力与外载之间的相关关系进行了探索,并找出了塑性区的合理扩展范围. 在此基础上,本文应用逐步回归分析,通过电算进行曲线拟合,获得了冻结壁厚度的简易计算公式:E=a(-1.21+0.54 lnP'). 最后,讨论了公式的安全系数问题。

This paper gives an overall description of the technical development of non- ferrous metal mining since the establishment of the People's Republie of China. Nu- merous figures presented show that China is rich in nonferrous metal resources and complete in the range of metals available, with a high rate of expansion in produc- tion as well as in the number of operating mines. Based on analysis of the present state of production and technology in more than 100 major nonferrous metal mines, an overview on...

This paper gives an overall description of the technical development of non- ferrous metal mining since the establishment of the People's Republie of China. Nu- merous figures presented show that China is rich in nonferrous metal resources and complete in the range of metals available, with a high rate of expansion in produc- tion as well as in the number of operating mines. Based on analysis of the present state of production and technology in more than 100 major nonferrous metal mines, an overview on underground roadway development, change in relative importance of different mining methods, development of open-pit mining technology, equip- ment levels of different categories of mines, as well as research and design work, is presented.

文章较全面地介绍了新中国成立以来,有色金属矿山技术发展的概况。列举了大量数字,有力地说明了我国有色金属资源丰富、品种齐全的特点;无论从生产规模还是矿山数目来说,发展速度都是很快的;并从全国一百多个重点有色金属矿山的生产技术现状进行分析,对地下开采矿山的井巷掘进、采矿方法的比重变化、露天开采工艺的发展,及各类型矿山的装备水平,科研和设计工作,作了扼要的综合性评述。

 
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