Methods: The dendritic cell subsets(DC1/CD11C+ and DC2/CD123+) in peripheral blood in 38 patients with AD and 35 healthy controls were detected by flow cytometry three-color immunofluorescent staining,their results were compared.
Methods Using three-color flow cytometry, we studied the phenotype expression of CD11c, CD123, CDla, CD80, CD86, CD40 and CD83 on the two subsets of DC(CD11c+DC and CD123+DC) in peripheral blood. The monocytes isolated from the blood were cultured with recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-4 (IL-4) and tumor necrosis factor alpha (TNF-α) from 20 patients with recurrent genital herpes (RGH) and 18 healthy controls.
Meanwhile three-color flow cytometry was applie to analyse the percentage of CD4+IFN-γ+IL-10- T, CD4+IFN-γ-IL-10+ T and CD4+IFN-γ+IL-10+ T subsets of lymphocytes from PBMCs in 10 active SLE and 6 control after 24-hours culture.
Methods: Three-color flow cytometric analysis to meature the subpopulation of peripheral blood CD4+CD25+ Regulatory T Cells andCD4+/CD8+ T lympphocytes from 26 Alopecia areata patients and 14 normal persons.
We investigated the number of peripheral blood CD4~+CD25~+ T cells and CD62L,CD28,CTLA-4 molecules expressing on these cells in patients on +21、 31、 41、 51、 61 day after allo-HSCT by three-color flow cytometry.
Methods The peripheral blood were collected from 104 patients with chronic hepatitis B and 30 healthy individuals,then CD4+CD45RA+,CD4+CD45RO+,CD8+CD45RA+ and CD8+CD45RO+ T lymphocyte subsets were detected by flow cytometry with three-color fluorescence technology.
Here we report a detailed protocol for two- and three-color fluorescent RNA in situ hybridization (ISH) and its combination with immunohistochemistry, or detection of bromodeoxyuridine (BrdU)-incorporated DNA after labeling.
Protocols for two- and three-color fluorescent RNA in situ hybridization of the main and accessory olfactory epithelia in mouse
Progress in the development of a three-color cadmium-vapor laser with discharge in a hollow cathode
Cells were stained with a combination of the tetramers and antibodies for CD28 and CD57 or a panel of TCR Vβ and analyzed by three-color flow cytometry.
The phenotypic changes in B cells isolated from such patients were compared with the markers on B cells isolated from HIV-seronegative individuals using three-color flow cytometry.
In this article, a hew method of color holography is suggested. Only a single wavelength laser is needed as light source, and any monochromatic holographic plate for photographic recording. The hologram obtained is then reconstructed by means of white light transmission. Experiments have been carried out using flower and three color circles as objects, the reconstructed pictures are presented here.
A new automatic decorating line involving circular three-color decorating machine, gold-decorating device and photoelectric lehr loader has been developed. Technical data and components of the machine are described.