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proteinaceous products
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  蛋白质类生化产品
     High performance capillary electrophoresis of proteinaceous products
     蛋白质类生化产品的高效毛细管电泳分析
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     Objective: To establish a high performance capillary electrophoresis (HPCE) method for optimal separation of proteinaceous products.
     目的:建立适合混合蛋白质分离分析的高效毛细管电泳方法。
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     High performance capillary electrophoresis of proteinaceous products
     蛋白质类生化产品的高效毛细管电泳分析
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  proteinaceous products
The inclusions may represent virus particles or proteinaceous products of the granular endoplasmic reticulum in which protein-synthetizing activities are probably increased to enormous extents.
      


Objective: To establish a high performance capillary electrophoresis (HPCE) method for optimal separation of proteinaceous products. Methods: Cytochrome C and lysozyme were selected as model samples ,and the some factors affecting electrophoretic separation were investigated, based on capillary zone electrophoresis(CZE) mode. Results and Conclusion:Three combined strategies for the optimal separation of cytochrome C and lysozyme were described: the reduction of protein wall adsorption, the optimization...

Objective: To establish a high performance capillary electrophoresis (HPCE) method for optimal separation of proteinaceous products. Methods: Cytochrome C and lysozyme were selected as model samples ,and the some factors affecting electrophoretic separation were investigated, based on capillary zone electrophoresis(CZE) mode. Results and Conclusion:Three combined strategies for the optimal separation of cytochrome C and lysozyme were described: the reduction of protein wall adsorption, the optimization for electrophoretic buffer system, and the normalization of HPCE qualitative and quantitative information.Successful application to phospholipase A2 purity analysis was demonstrated.The conclusion was obvious that HPCE should provide a simple, quick and effective analytical tool for proteinaceous drugs quality control.

目的:建立适合混合蛋白质分离分析的高效毛细管电泳方法。方法:采用高效毛细管电泳中自由区带电泳分离模式,以标准蛋白质细胞色素C与溶菌酶为分析对象,对影响电泳分离的因素进行了考察。结果与结论:通过减少蛋白质管壁吸附、优选缓冲液体系及定性定量信息校正,使细胞色素C与溶菌酶获得了理想的分离,同时成功地应用于磷脂酶A2的优化分离与纯度分析,为蛋白质类生化药物的质量控制提供了简便、快速、有效的检测手段

 
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